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Q9BT25

- HAUS8_HUMAN

UniProt

Q9BT25 - HAUS8_HUMAN

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Protein

HAUS augmin-like complex subunit 8

Gene

HAUS8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.3 Publications

GO - Biological processi

  1. centrosome organization Source: UniProtKB
  2. mitotic nuclear division Source: UniProtKB-KW
  3. spindle assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
HAUS augmin-like complex subunit 8
Alternative name(s):
HEC1/NDC80-interacting centrosome-associated protein 1
Sarcoma antigen NY-SAR-48
Gene namesi
Name:HAUS8
Synonyms:HICE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:30532. HAUS8.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasmcytoskeletonspindle. Cytoplasmcytoskeletonspindle pole
Note: During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, additionally associates with the spindle midzone and midbody, respectively.

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. HAUS complex Source: UniProtKB
  4. microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165393466.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 410409HAUS augmin-like complex subunit 8PRO_0000319937Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei105 – 1051Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9BT25.
PaxDbiQ9BT25.
PRIDEiQ9BT25.

PTM databases

PhosphoSiteiQ9BT25.

Expressioni

Gene expression databases

BgeeiQ9BT25.
ExpressionAtlasiQ9BT25. baseline and differential.
GenevestigatoriQ9BT25.

Organism-specific databases

HPAiHPA039406.

Interactioni

Subunit structurei

Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Associates with microtubules. The interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. It is unclear whether this interaction is direct or indirect.2 Publications

Protein-protein interaction databases

BioGridi125017. 14 interactions.
DIPiDIP-48834N.
IntActiQ9BT25. 10 interactions.
STRINGi9606.ENSP00000253669.

Structurei

3D structure databases

ProteinModelPortaliQ9BT25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili156 – 20853Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the HAUS8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG47763.
GeneTreeiENSGT00390000010974.
HOGENOMiHOG000112854.
InParanoidiQ9BT25.
KOiK16591.
OMAiESRYLQY.
OrthoDBiEOG7JHM6B.
PhylomeDBiQ9BT25.
TreeFamiTF332998.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9BT25-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSSGRGAG KPATGPTNSS SAKKKDKRVQ GGRVIESRYL QYEKKTTQKA
60 70 80 90 100
PAGDGSQTRG KMSEGGRKSS LLQKSKADSS GVGKGDLQST LLEGHGTAPP
110 120 130 140 150
DLDLSAINDK SIVKKTPQLA KTISKKPEST SFSAPRKKSP DLSEAMEMME
160 170 180 190 200
SQTLLLTLLS VKMENNLAEF ERRAEKNLLI MCKEKEKLQK KAHELKRRLL
210 220 230 240 250
LSQRKRELAD VLDAQIEMLS PFEAVATRFK EQYRTFATAL DTTRHELPVR
260 270 280 290 300
SIHLEGDGQQ LLDALQHELV TTQRLLGELD VGDSEENVQV LDLLSELKDV
310 320 330 340 350
TAKKDLELRR SFAQVLELSA EASKEAALAN QEVWEETQGM APPSRWYFNQ
360 370 380 390 400
DSACRESGGA PKNTPLSEDD NPGASSAPAQ ATFISPSEDF SSSSQAEVPP
410
SLSRSGRDLS
Length:410
Mass (Da):44,857
Last modified:May 5, 2009 - v3
Checksum:i05D7B175523E5DA5
GO
Isoform 2 (identifier: Q9BT25-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Note: No experimental confirmation available.

Show »
Length:349
Mass (Da):38,464
Checksum:iA16103E23892C1AC
GO
Isoform 3 (identifier: Q9BT25-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-77: Missing.

Note: Gene prediction based on EST data.

Show »
Length:409
Mass (Da):44,786
Checksum:i414269CC2C9C254F
GO

Sequence cautioni

The sequence AAH10176.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAO65172.1 differs from that shown. Reason: Frameshift at positions 222, 241, 269, 278 and 279. Curated
The sequence AAO65172.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381R → P in AAH04398. (PubMed:15489334)Curated
Sequence conflicti88 – 881Q → QLQ no nucleotide entry (PubMed:18362163)Curated
Sequence conflicti268 – 2681E → D in AAO65172. (PubMed:12601173)Curated
Sequence conflicti272 – 2721T → I no nucleotide entry (PubMed:18362163)Curated
Sequence conflicti272 – 2721T → L in AAO65172. (PubMed:12601173)Curated
Sequence conflicti275 – 2751L → F no nucleotide entry (PubMed:18362163)Curated
Sequence conflicti354 – 3552Missing no nucleotide entry (PubMed:18362163)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti83 – 831G → R.2 Publications
Corresponds to variant rs1130222 [ dbSNP | Ensembl ].
VAR_039056

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6161Missing in isoform 2. CuratedVSP_031543Add
BLAST
Alternative sequencei77 – 771Missing in isoform 3. CuratedVSP_047167

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK295994 mRNA. Translation: BAG58769.1.
AC020908 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84570.1.
CH471106 Genomic DNA. Translation: EAW84571.1.
AY211919 mRNA. Translation: AAO65172.1. Sequence problems.
BC004398 mRNA. Translation: AAH04398.1.
BC010176 mRNA. Translation: AAH10176.1. Different initiation.
CCDSiCCDS32948.1. [Q9BT25-1]
CCDS46009.1. [Q9BT25-3]
RefSeqiNP_001011699.1. NM_001011699.1. [Q9BT25-3]
NP_219485.1. NM_033417.1. [Q9BT25-1]
UniGeneiHs.404088.

Genome annotation databases

EnsembliENST00000253669; ENSP00000253669; ENSG00000131351. [Q9BT25-1]
ENST00000448593; ENSP00000395298; ENSG00000131351. [Q9BT25-3]
ENST00000593360; ENSP00000470829; ENSG00000131351. [Q9BT25-2]
GeneIDi93323.
KEGGihsa:93323.
UCSCiuc002nfe.3. human. [Q9BT25-1]

Polymorphism databases

DMDMi229462967.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK295994 mRNA. Translation: BAG58769.1 .
AC020908 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84570.1 .
CH471106 Genomic DNA. Translation: EAW84571.1 .
AY211919 mRNA. Translation: AAO65172.1 . Sequence problems.
BC004398 mRNA. Translation: AAH04398.1 .
BC010176 mRNA. Translation: AAH10176.1 . Different initiation.
CCDSi CCDS32948.1. [Q9BT25-1 ]
CCDS46009.1. [Q9BT25-3 ]
RefSeqi NP_001011699.1. NM_001011699.1. [Q9BT25-3 ]
NP_219485.1. NM_033417.1. [Q9BT25-1 ]
UniGenei Hs.404088.

3D structure databases

ProteinModelPortali Q9BT25.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 125017. 14 interactions.
DIPi DIP-48834N.
IntActi Q9BT25. 10 interactions.
STRINGi 9606.ENSP00000253669.

PTM databases

PhosphoSitei Q9BT25.

Polymorphism databases

DMDMi 229462967.

Proteomic databases

MaxQBi Q9BT25.
PaxDbi Q9BT25.
PRIDEi Q9BT25.

Protocols and materials databases

DNASUi 93323.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000253669 ; ENSP00000253669 ; ENSG00000131351 . [Q9BT25-1 ]
ENST00000448593 ; ENSP00000395298 ; ENSG00000131351 . [Q9BT25-3 ]
ENST00000593360 ; ENSP00000470829 ; ENSG00000131351 . [Q9BT25-2 ]
GeneIDi 93323.
KEGGi hsa:93323.
UCSCi uc002nfe.3. human. [Q9BT25-1 ]

Organism-specific databases

CTDi 93323.
GeneCardsi GC19M017160.
H-InvDB HIX0014879.
HGNCi HGNC:30532. HAUS8.
HPAi HPA039406.
MIMi 613434. gene.
neXtProti NX_Q9BT25.
PharmGKBi PA165393466.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG47763.
GeneTreei ENSGT00390000010974.
HOGENOMi HOG000112854.
InParanoidi Q9BT25.
KOi K16591.
OMAi ESRYLQY.
OrthoDBi EOG7JHM6B.
PhylomeDBi Q9BT25.
TreeFami TF332998.

Miscellaneous databases

ChiTaRSi HAUS8. human.
GenomeRNAii 93323.
NextBioi 78044.
PROi Q9BT25.
SOURCEi Search...

Gene expression databases

Bgeei Q9BT25.
ExpressionAtlasi Q9BT25. baseline and differential.
Genevestigatori Q9BT25.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells."
    Wu G., Lin Y.-T., Wei R., Chen Y., Shan Z., Lee W.-H.
    Mol. Cell. Biol. 28:3652-3662(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, VARIANT ARG-83.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Subthalamic nucleus.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-283 (ISOFORM 1).
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-410 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 152-410 (ISOFORMS 1/2), VARIANT ARG-83.
    Tissue: Eye and Placenta.
  7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
  10. "The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells."
    Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C., Goshima G.
    Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHAUS8_HUMAN
AccessioniPrimary (citable) accession number: Q9BT25
Secondary accession number(s): B4DJA7
, C9JBZ4, Q49AC4, Q86WF0, Q96FX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 5, 2009
Last modified: November 26, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3