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Q9BT25 (HAUS8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HAUS augmin-like complex subunit 8
Alternative name(s):
HEC1/NDC80-interacting centrosome-associated protein 1
Sarcoma antigen NY-SAR-48
Gene names
Name:HAUS8
Synonyms:HICE1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Ref.1 Ref.9 Ref.10

Subunit structure

Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Associates with microtubules. The interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. It is unclear whether this interaction is direct or indirect. Ref.9 Ref.10

Subcellular location

Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasmcytoskeletonspindle. Cytoplasmcytoskeletonspindle pole. Note: During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, additionally associates with the spindle midzone and midbody, respectively. Ref.1 Ref.9 Ref.10

Sequence similarities

Belongs to the HAUS8 family.

Sequence caution

The sequence AAH10176.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAO65172.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAO65172.1 differs from that shown. Reason: Frameshift at positions 222, 241, 269, 278 and 279.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BT25-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BT25-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9BT25-3)

The sequence of this isoform differs from the canonical sequence as follows:
     77-77: Missing.
Note: Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 410409HAUS augmin-like complex subunit 8
PRO_0000319937

Regions

Coiled coil156 – 20853 Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.8
Modified residue1051Phosphoserine Ref.7

Natural variations

Alternative sequence1 – 6161Missing in isoform 2.
VSP_031543
Alternative sequence771Missing in isoform 3.
VSP_047167
Natural variant831G → R. Ref.1 Ref.6
Corresponds to variant rs1130222 [ dbSNP | Ensembl ].
VAR_039056

Experimental info

Sequence conflict381R → P in AAH04398. Ref.6
Sequence conflict881Q → QLQ no nucleotide entry Ref.1
Sequence conflict2681E → D in AAO65172. Ref.5
Sequence conflict2721T → I no nucleotide entry Ref.1
Sequence conflict2721T → L in AAO65172. Ref.5
Sequence conflict2751L → F no nucleotide entry Ref.1
Sequence conflict354 – 3552Missing no nucleotide entry Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 3.
Checksum: 05D7B175523E5DA5

FASTA41044,857
        10         20         30         40         50         60 
MADSSGRGAG KPATGPTNSS SAKKKDKRVQ GGRVIESRYL QYEKKTTQKA PAGDGSQTRG 

        70         80         90        100        110        120 
KMSEGGRKSS LLQKSKADSS GVGKGDLQST LLEGHGTAPP DLDLSAINDK SIVKKTPQLA 

       130        140        150        160        170        180 
KTISKKPEST SFSAPRKKSP DLSEAMEMME SQTLLLTLLS VKMENNLAEF ERRAEKNLLI 

       190        200        210        220        230        240 
MCKEKEKLQK KAHELKRRLL LSQRKRELAD VLDAQIEMLS PFEAVATRFK EQYRTFATAL 

       250        260        270        280        290        300 
DTTRHELPVR SIHLEGDGQQ LLDALQHELV TTQRLLGELD VGDSEENVQV LDLLSELKDV 

       310        320        330        340        350        360 
TAKKDLELRR SFAQVLELSA EASKEAALAN QEVWEETQGM APPSRWYFNQ DSACRESGGA 

       370        380        390        400        410 
PKNTPLSEDD NPGASSAPAQ ATFISPSEDF SSSSQAEVPP SLSRSGRDLS 

« Hide

Isoform 2 [UniParc].

Checksum: A16103E23892C1AC
Show »

FASTA34938,464
Isoform 3 [UniParc].

Checksum: 414269CC2C9C254F
Show »

FASTA40944,786

References

« Hide 'large scale' references
[1]"Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells."
Wu G., Lin Y.-T., Wei R., Chen Y., Shan Z., Lee W.-H.
Mol. Cell. Biol. 28:3652-3662(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, VARIANT ARG-83.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Subthalamic nucleus.
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Immunomic analysis of human sarcoma."
Lee S.-Y., Obata Y., Yoshida M., Stockert E., Williamson B., Jungbluth A.A., Chen Y.-T., Old L.J., Scanlan M.J.
Proc. Natl. Acad. Sci. U.S.A. 100:2651-2656(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-283 (ISOFORM 1).
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-410 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 152-410 (ISOFORMS 1/2), VARIANT ARG-83.
Tissue: Eye and Placenta.
[7]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[9]"HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle integrity."
Lawo S., Bashkurov M., Mullin M., Ferreria M.G., Kittler R., Habermann B., Tagliaferro A., Poser I., Hutchins J.R.A., Hegemann B., Pinchev D., Buchholz F., Peters J.-M., Hyman A.A., Gingras A.-C., Pelletier L.
Curr. Biol. 19:816-826(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
[10]"The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells."
Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C., Goshima G.
Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK295994 mRNA. Translation: BAG58769.1.
AC020908 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84570.1.
CH471106 Genomic DNA. Translation: EAW84571.1.
AY211919 mRNA. Translation: AAO65172.1. Sequence problems.
BC004398 mRNA. Translation: AAH04398.1.
BC010176 mRNA. Translation: AAH10176.1. Different initiation.
RefSeqNP_001011699.1. NM_001011699.1.
NP_219485.1. NM_033417.1.
UniGeneHs.404088.

3D structure databases

ProteinModelPortalQ9BT25.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125017. 10 interactions.
DIPDIP-48834N.
IntActQ9BT25. 9 interactions.
STRING9606.ENSP00000253669.

PTM databases

PhosphoSiteQ9BT25.

Polymorphism databases

DMDM229462967.

Proteomic databases

PaxDbQ9BT25.
PRIDEQ9BT25.

Protocols and materials databases

DNASU93323.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000253669; ENSP00000253669; ENSG00000131351. [Q9BT25-1]
ENST00000448593; ENSP00000395298; ENSG00000131351. [Q9BT25-3]
ENST00000593360; ENSP00000470829; ENSG00000131351. [Q9BT25-2]
GeneID93323.
KEGGhsa:93323.
UCSCuc002nfe.3. human. [Q9BT25-1]

Organism-specific databases

CTD93323.
GeneCardsGC19M017160.
H-InvDBHIX0014879.
HGNCHGNC:30532. HAUS8.
HPAHPA039406.
MIM613434. gene.
neXtProtNX_Q9BT25.
PharmGKBPA165393466.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG47763.
HOGENOMHOG000112854.
InParanoidQ9BT25.
KOK16591.
OMAESRYLQY.
OrthoDBEOG7JHM6B.
PhylomeDBQ9BT25.
TreeFamTF332998.

Gene expression databases

ArrayExpressQ9BT25.
BgeeQ9BT25.
GenevestigatorQ9BT25.

Family and domain databases

ProtoNetSearch...

Other

ChiTaRSHAUS8. human.
GenomeRNAi93323.
NextBio78044.
PROQ9BT25.
SOURCESearch...

Entry information

Entry nameHAUS8_HUMAN
AccessionPrimary (citable) accession number: Q9BT25
Secondary accession number(s): B4DJA7 expand/collapse secondary AC list , C9JBZ4, Q49AC4, Q86WF0, Q96FX3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 5, 2009
Last modified: March 19, 2014
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM