Q9BT25 (HAUS8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HAUS augmin-like complex subunit 8

Alternative name(s):
HEC1/NDC80-interacting centrosome-associated protein 1
Sarcoma antigen NY-SAR-48

Gene names

Name:	HAUS8
Synonyms:	HICE1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Ref.1 Ref.7 Ref.8
Subunit structure	Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Associates with microtubules. The interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. It is unclear whether this interaction is direct or indirect. Ref.7 Ref.8
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, additionally associates with the spindle midzone and midbody, respectively. Ref.1 Ref.7 Ref.8
Sequence similarities	Belongs to the HAUS8 family.
Sequence caution	The sequence AAH10176.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAO65172.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAO65172.1 differs from that shown. Reason: Frameshift at positions 222, 241, 269, 278 and 279.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW centrosome organization Inferred from mutant phenotype Ref.7. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW spindle assembly Inferred from mutant phenotype Ref.8Ref.7. Source: UniProtKB
Cellular_component	HAUS complex Inferred from direct assay Ref.8Ref.7. Source: UniProtKB centrosome Inferred from direct assay PubMed 21399614. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BT25-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BT25-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	HAUS augmin-like complex subunit 8		PRO_0000319937
Regions
	Coiled coil	156 – 208	53	Potential
Amino acid modifications
	Modified residue	105	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 61	61	Missing in isoform 2.		VSP_031543
	Natural variant	83	1	G → R. Ref.1 Ref.5 Corresponds to variant rs1130222 [ dbSNP | Ensembl ].		VAR_039056
Experimental info
	Sequence conflict	38	1	R → P in AAH04398. Ref.5
	Sequence conflict	88	1	Q → QLQ no nucleotide entry Ref.1
	Sequence conflict	268	1	E → D in AAO65172. Ref.4
	Sequence conflict	272	1	T → I no nucleotide entry Ref.1
	Sequence conflict	272	1	T → L in AAO65172. Ref.4
	Sequence conflict	275	1	L → F no nucleotide entry Ref.1
	Sequence conflict	354 – 355	2	Missing no nucleotide entry Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 3. Checksum: 05D7B175523E5DA5	FASTA	410	44,857
	10 20 30 40 50 60 MADSSGRGAG KPATGPTNSS SAKKKDKRVQ GGRVIESRYL QYEKKTTQKA PAGDGSQTRG 70 80 90 100 110 120 KMSEGGRKSS LLQKSKADSS GVGKGDLQST LLEGHGTAPP DLDLSAINDK SIVKKTPQLA 130 140 150 160 170 180 KTISKKPEST SFSAPRKKSP DLSEAMEMME SQTLLLTLLS VKMENNLAEF ERRAEKNLLI 190 200 210 220 230 240 MCKEKEKLQK KAHELKRRLL LSQRKRELAD VLDAQIEMLS PFEAVATRFK EQYRTFATAL 250 260 270 280 290 300 DTTRHELPVR SIHLEGDGQQ LLDALQHELV TTQRLLGELD VGDSEENVQV LDLLSELKDV 310 320 330 340 350 360 TAKKDLELRR SFAQVLELSA EASKEAALAN QEVWEETQGM APPSRWYFNQ DSACRESGGA 370 380 390 400 410 PKNTPLSEDD NPGASSAPAQ ATFISPSEDF SSSSQAEVPP SLSRSGRDLS
	Isoform 2 [UniParc]. Checksum: A16103E23892C1AC	FASTA	349	38,464
	10 20 30 40 50 60 MSEGGRKSSL LQKSKADSSG VGKGDLQSTL LEGHGTAPPD LDLSAINDKS IVKKTPQLAK 70 80 90 100 110 120 TISKKPESTS FSAPRKKSPD LSEAMEMMES QTLLLTLLSV KMENNLAEFE RRAEKNLLIM 130 140 150 160 170 180 CKEKEKLQKK AHELKRRLLL SQRKRELADV LDAQIEMLSP FEAVATRFKE QYRTFATALD 190 200 210 220 230 240 TTRHELPVRS IHLEGDGQQL LDALQHELVT TQRLLGELDV GDSEENVQVL DLLSELKDVT 250 260 270 280 290 300 AKKDLELRRS FAQVLELSAE ASKEAALANQ EVWEETQGMA PPSRWYFNQD SACRESGGAP 310 320 330 340 KNTPLSEDDN PGASSAPAQA TFISPSEDFS SSSQAEVPPS LSRSGRDLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells." Wu G., Lin Y.-T., Wei R., Chen Y., Shan Z., Lee W.-H. Mol. Cell. Biol. 28:3652-3662(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, VARIANT ARG-83.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Subthalamic nucleus.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Immunomic analysis of human sarcoma." Lee S.-Y., Obata Y., Yoshida M., Stockert E., Williamson B., Jungbluth A.A., Chen Y.-T., Old L.J., Scanlan M.J. Proc. Natl. Acad. Sci. U.S.A. 100:2651-2656(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-283 (ISOFORM 1).
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-410 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 152-410 (ISOFORMS 1/2), VARIANT ARG-83. Tissue: Eye and Placenta.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle integrity." Lawo S., Bashkurov M., Mullin M., Ferreria M.G., Kittler R., Habermann B., Tagliaferro A., Poser I., Hutchins J.R.A., Hegemann B., Pinchev D., Buchholz F., Peters J.-M., Hyman A.A., Gingras A.-C., Pelletier L. Curr. Biol. 19:816-826(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
[8]	"The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells." Uehara R., Nozawa R.-S., Tomioka A., Petry S., Vale R.D., Obuse C., Goshima G. Proc. Natl. Acad. Sci. U.S.A. 106:6998-7003(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE HAUS AUGMIN-LIKE COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK295994 mRNA. Translation: BAG58769.1. CH471106 Genomic DNA. Translation: EAW84570.1. CH471106 Genomic DNA. Translation: EAW84571.1. AY211919 mRNA. Translation: AAO65172.1. Sequence problems. BC004398 mRNA. Translation: AAH04398.1. BC010176 mRNA. Translation: AAH10176.1. Different initiation.
IPI	IPI00305490. IPI00885183.
RefSeq	NP_219485.1. NM_033417.1.
UniGene	Hs.404088.
3D structure databases
ProteinModelPortal	Q9BT25.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48834N.
IntAct	Q9BT25. 9 interactions.
STRING	9606.ENSP00000253669.
PTM databases
PhosphoSite	Q9BT25.
Polymorphism databases
DMDM	229462967.
Proteomic databases
PaxDb	Q9BT25.
PRIDE	Q9BT25.
Protocols and materials databases
DNASU	93323.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000253669; ENSP00000253669; ENSG00000131351. ENST00000593360; ENSP00000470829; ENSG00000131351.
GeneID	93323.
KEGG	hsa:93323.
UCSC	uc002nfe.3. human.
Organism-specific databases
CTD	93323.
GeneCards	GC19M017160.
H-InvDB	HIX0014879.
HGNC	HGNC:30532. HAUS8.
HPA	HPA039406.
MIM	613434. gene.
neXtProt	NX_Q9BT25.
PharmGKB	PA165393466.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47763.
HOGENOM	HOG000112854.
InParanoid	Q9BT25.
KO	K16591.
OMA	SRYLQYE.
OrthoDB	EOG4RR6HJ.
PhylomeDB	Q9BT25.
Gene expression databases
ArrayExpress	Q9BT25.
Bgee	Q9BT25.
Genevestigator	Q9BT25.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	HAUS8. human.
GenomeRNAi	93323.
NextBio	78044.
SOURCE	Search...

Entry information

Entry name

HAUS8_HUMAN

Accession

Primary (citable) accession number: Q9BT25
Secondary accession number(s): B4DJA7 , Q49AC4, Q86WF0, Q96FX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.