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Protein

HAUS augmin-like complex subunit 8

Gene

HAUS8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.3 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centrosome organization Source: UniProtKB
  • G2/M transition of mitotic cell cycle Source: Reactome
  • mitotic nuclear division Source: UniProtKB-KW
  • spindle assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131351-MONOMER.
ReactomeiR-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
HAUS augmin-like complex subunit 8
Alternative name(s):
HEC1/NDC80-interacting centrosome-associated protein 1
Sarcoma antigen NY-SAR-48
Gene namesi
Name:HAUS8
Synonyms:HICE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:30532. HAUS8.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytosol Source: Reactome
  • HAUS complex Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi93323.
OpenTargetsiENSG00000131351.
PharmGKBiPA165393466.

Polymorphism and mutation databases

BioMutaiHAUS8.
DMDMi229462967.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003199372 – 410HAUS augmin-like complex subunit 8Add BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei105PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BT25.
MaxQBiQ9BT25.
PaxDbiQ9BT25.
PeptideAtlasiQ9BT25.
PRIDEiQ9BT25.

PTM databases

iPTMnetiQ9BT25.
PhosphoSitePlusiQ9BT25.

Expressioni

Gene expression databases

BgeeiENSG00000131351.
ExpressionAtlasiQ9BT25. baseline and differential.
GenevisibleiQ9BT25. HS.

Organism-specific databases

HPAiHPA039406.

Interactioni

Subunit structurei

Component of the HAUS augmin-like complex. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly. Associates with microtubules. The interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. It is unclear whether this interaction is direct or indirect.2 Publications

Protein-protein interaction databases

BioGridi125017. 54 interactors.
DIPiDIP-48834N.
IntActiQ9BT25. 44 interactors.
STRINGi9606.ENSP00000253669.

Structurei

3D structure databases

ProteinModelPortaliQ9BT25.
SMRiQ9BT25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili156 – 208Sequence analysisAdd BLAST53

Sequence similaritiesi

Belongs to the HAUS8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IEK2. Eukaryota.
ENOG4112BWM. LUCA.
GeneTreeiENSGT00390000010974.
HOGENOMiHOG000112854.
InParanoidiQ9BT25.
KOiK16591.
OMAiEMMESQT.
OrthoDBiEOG091G0B15.
PhylomeDBiQ9BT25.
TreeFamiTF332998.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BT25-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSSGRGAG KPATGPTNSS SAKKKDKRVQ GGRVIESRYL QYEKKTTQKA
60 70 80 90 100
PAGDGSQTRG KMSEGGRKSS LLQKSKADSS GVGKGDLQST LLEGHGTAPP
110 120 130 140 150
DLDLSAINDK SIVKKTPQLA KTISKKPEST SFSAPRKKSP DLSEAMEMME
160 170 180 190 200
SQTLLLTLLS VKMENNLAEF ERRAEKNLLI MCKEKEKLQK KAHELKRRLL
210 220 230 240 250
LSQRKRELAD VLDAQIEMLS PFEAVATRFK EQYRTFATAL DTTRHELPVR
260 270 280 290 300
SIHLEGDGQQ LLDALQHELV TTQRLLGELD VGDSEENVQV LDLLSELKDV
310 320 330 340 350
TAKKDLELRR SFAQVLELSA EASKEAALAN QEVWEETQGM APPSRWYFNQ
360 370 380 390 400
DSACRESGGA PKNTPLSEDD NPGASSAPAQ ATFISPSEDF SSSSQAEVPP
410
SLSRSGRDLS
Length:410
Mass (Da):44,857
Last modified:May 5, 2009 - v3
Checksum:i05D7B175523E5DA5
GO
Isoform 2 (identifier: Q9BT25-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.

Note: No experimental confirmation available.
Show »
Length:349
Mass (Da):38,464
Checksum:iA16103E23892C1AC
GO
Isoform 3 (identifier: Q9BT25-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-77: Missing.

Note: Gene prediction based on EST data.
Show »
Length:409
Mass (Da):44,786
Checksum:i414269CC2C9C254F
GO

Sequence cautioni

The sequence AAH10176 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAO65172 differs from that shown. Reason: Frameshift at positions 222, 241, 269, 278 and 279.Curated
The sequence AAO65172 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38R → P in AAH04398 (PubMed:15489334).Curated1
Sequence conflicti88Q → QLQ no nucleotide entry (PubMed:18362163).Curated1
Sequence conflicti268E → D in AAO65172 (PubMed:12601173).Curated1
Sequence conflicti272T → I no nucleotide entry (PubMed:18362163).Curated1
Sequence conflicti272T → L in AAO65172 (PubMed:12601173).Curated1
Sequence conflicti275L → F no nucleotide entry (PubMed:18362163).Curated1
Sequence conflicti354 – 355Missing no nucleotide entry (PubMed:18362163).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03905683G → R.2 PublicationsCorresponds to variant rs1130222dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0315431 – 61Missing in isoform 2. CuratedAdd BLAST61
Alternative sequenceiVSP_04716777Missing in isoform 3. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK295994 mRNA. Translation: BAG58769.1.
AC020908 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84570.1.
CH471106 Genomic DNA. Translation: EAW84571.1.
AY211919 mRNA. Translation: AAO65172.1. Sequence problems.
BC004398 mRNA. Translation: AAH04398.1.
BC010176 mRNA. Translation: AAH10176.1. Different initiation.
CCDSiCCDS32948.1. [Q9BT25-1]
CCDS46009.1. [Q9BT25-3]
RefSeqiNP_001011699.1. NM_001011699.1. [Q9BT25-3]
NP_219485.1. NM_033417.1. [Q9BT25-1]
UniGeneiHs.404088.

Genome annotation databases

EnsembliENST00000253669; ENSP00000253669; ENSG00000131351. [Q9BT25-1]
ENST00000448593; ENSP00000395298; ENSG00000131351. [Q9BT25-3]
ENST00000593360; ENSP00000470829; ENSG00000131351. [Q9BT25-2]
GeneIDi93323.
KEGGihsa:93323.
UCSCiuc002nfe.4. human. [Q9BT25-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK295994 mRNA. Translation: BAG58769.1.
AC020908 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84570.1.
CH471106 Genomic DNA. Translation: EAW84571.1.
AY211919 mRNA. Translation: AAO65172.1. Sequence problems.
BC004398 mRNA. Translation: AAH04398.1.
BC010176 mRNA. Translation: AAH10176.1. Different initiation.
CCDSiCCDS32948.1. [Q9BT25-1]
CCDS46009.1. [Q9BT25-3]
RefSeqiNP_001011699.1. NM_001011699.1. [Q9BT25-3]
NP_219485.1. NM_033417.1. [Q9BT25-1]
UniGeneiHs.404088.

3D structure databases

ProteinModelPortaliQ9BT25.
SMRiQ9BT25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125017. 54 interactors.
DIPiDIP-48834N.
IntActiQ9BT25. 44 interactors.
STRINGi9606.ENSP00000253669.

PTM databases

iPTMnetiQ9BT25.
PhosphoSitePlusiQ9BT25.

Polymorphism and mutation databases

BioMutaiHAUS8.
DMDMi229462967.

Proteomic databases

EPDiQ9BT25.
MaxQBiQ9BT25.
PaxDbiQ9BT25.
PeptideAtlasiQ9BT25.
PRIDEiQ9BT25.

Protocols and materials databases

DNASUi93323.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253669; ENSP00000253669; ENSG00000131351. [Q9BT25-1]
ENST00000448593; ENSP00000395298; ENSG00000131351. [Q9BT25-3]
ENST00000593360; ENSP00000470829; ENSG00000131351. [Q9BT25-2]
GeneIDi93323.
KEGGihsa:93323.
UCSCiuc002nfe.4. human. [Q9BT25-1]

Organism-specific databases

CTDi93323.
DisGeNETi93323.
GeneCardsiHAUS8.
H-InvDBHIX0014879.
HGNCiHGNC:30532. HAUS8.
HPAiHPA039406.
MIMi613434. gene.
neXtProtiNX_Q9BT25.
OpenTargetsiENSG00000131351.
PharmGKBiPA165393466.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEK2. Eukaryota.
ENOG4112BWM. LUCA.
GeneTreeiENSGT00390000010974.
HOGENOMiHOG000112854.
InParanoidiQ9BT25.
KOiK16591.
OMAiEMMESQT.
OrthoDBiEOG091G0B15.
PhylomeDBiQ9BT25.
TreeFamiTF332998.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131351-MONOMER.
ReactomeiR-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-8854518. AURKA Activation by TPX2.

Miscellaneous databases

ChiTaRSiHAUS8. human.
GenomeRNAii93323.
PROiQ9BT25.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131351.
ExpressionAtlasiQ9BT25. baseline and differential.
GenevisibleiQ9BT25. HS.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiHAUS8_HUMAN
AccessioniPrimary (citable) accession number: Q9BT25
Secondary accession number(s): B4DJA7
, C9JBZ4, Q49AC4, Q86WF0, Q96FX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.