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Protein

Protein canopy homolog 3

Gene

CNPY3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Toll-like receptor (TLR)-specific co-chaperone for HSP90B1. Required for proper TLR folding, except that of TLR3, and hence controls TLR exit from the endoplasmic reticulum. Consequently, required for both innate and adaptive immune responses (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-HSA-1679131. Trafficking and processing of endosomal TLR.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein canopy homolog 3
Alternative name(s):
CTG repeat protein 4a
Expanded repeat-domain protein CAG/CTG 5
Protein associated with TLR4
Trinucleotide repeat-containing gene 5 protein
Gene namesi
Name:CNPY3
Synonyms:CTG4A, ERDA5, PRAT4A, TNRC5
ORF Names:HSPC084, UNQ1934/PRO4409
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:11968. CNPY3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162382601.

Polymorphism and mutation databases

BioMutaiCNPY3.
DMDMi74752319.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Chaini31 – 278248Protein canopy homolog 3PRO_0000313780Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 206By similarity
Disulfide bondi52 ↔ 194By similarity
Disulfide bondi104 ↔ 166By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ9BT09.
MaxQBiQ9BT09.
PaxDbiQ9BT09.
PRIDEiQ9BT09.

PTM databases

iPTMnetiQ9BT09.
PhosphoSiteiQ9BT09.

Expressioni

Gene expression databases

BgeeiQ9BT09.
CleanExiHS_CNPY3.
ExpressionAtlasiQ9BT09. baseline and differential.
GenevisibleiQ9BT09. HS.

Organism-specific databases

HPAiHPA016560.

Interactioni

Subunit structurei

Interacts with HSP90B1; this interaction is disrupted in the presence of ATP. Interacts with TLR1, TLR2, TLR4 and TLR9 (By similarity). Strongest interaction with TLR4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi115934. 25 interactions.
IntActiQ9BT09. 3 interactions.
STRINGi9606.ENSP00000361926.

Structurei

3D structure databases

ProteinModelPortaliQ9BT09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 271225Saposin B-typeAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili153 – 17927Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the canopy family.Curated
Contains 1 saposin B-type domain.Curated

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG4052. Eukaryota.
ENOG4111GVI. LUCA.
GeneTreeiENSGT00390000014072.
HOGENOMiHOG000030912.
HOVERGENiHBG107736.
InParanoidiQ9BT09.
OMAiDLTQFLC.
OrthoDBiEOG7SBNPF.
PhylomeDBiQ9BT09.
TreeFamiTF318951.

Family and domain databases

InterProiIPR021852. DUF3456.
[Graphical view]
PfamiPF11938. DUF3456. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BT09-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSMPEPASR CLLLLPLLLL LLLLLPAPEL GPSQAGAEEN DWVRLPSKCE
60 70 80 90 100
VCKYVAVELK SAFEETGKTK EVIGTGYGIL DQKASGVKYT KSDLRLIEVT
110 120 130 140 150
ETICKRLLDY SLHKERTGSN RFAKGMSETF ETLHNLVHKG VKVVMDIPYE
160 170 180 190 200
LWNETSAEVA DLKKQCDVLV EEFEEVIEDW YRNHQEEDLT EFLCANHVLK
210 220 230 240 250
GKDTSCLAEQ WSGKKGDTAA LGGKKSKKKS SRAKAAGGRS SSSKQRKELG
260 270
GLEGDPSPEE DEGIQKASPL THSPPDEL
Length:278
Mass (Da):30,748
Last modified:June 1, 2001 - v1
Checksum:i07587320D316E0CE
GO
Isoform 2 (identifier: Q9BT09-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     51-54: VCKY → GTCG
     55-278: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:54
Mass (Da):5,773
Checksum:i6345B76C85D1DD60
GO

Sequence cautioni

The sequence AAB91442.1 differs from that shown. Reason: Frameshift at position 109. Curated
The sequence AAF28907.1 differs from that shown. Reason: Frameshift at position 252. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti272 – 2721H → P in BAC03406 (Ref. 5) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti145 – 1451M → I.
Corresponds to variant rs1063252 [ dbSNP | Ensembl ].
VAR_037731
Natural varianti231 – 2311S → I.
Corresponds to variant rs9471969 [ dbSNP | Ensembl ].
VAR_037732

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei51 – 544VCKY → GTCG in isoform 2. 1 PublicationVSP_030132
Alternative sequencei55 – 278224Missing in isoform 2. 1 PublicationVSP_030133Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358960 mRNA. Translation: AAQ89319.1.
AL035587 Genomic DNA. Translation: CAB75301.1.
BC004423 mRNA. Translation: AAH04423.1.
BC008133 mRNA. No translation available.
BC008898 mRNA. Translation: AAH08898.1.
BC008961 mRNA. Translation: AAH08961.1.
BC022093 mRNA. Translation: AAH22093.3.
U80744 mRNA. Translation: AAB91442.1. Frameshift.
AF161347 mRNA. Translation: AAF28907.1. Frameshift.
AK090425 mRNA. Translation: BAC03406.1.
CCDSiCCDS4875.1. [Q9BT09-1]
RefSeqiNP_006577.2. NM_006586.4. [Q9BT09-1]
UniGeneiHs.414099.

Genome annotation databases

EnsembliENST00000372836; ENSP00000361926; ENSG00000137161. [Q9BT09-1]
GeneIDi10695.
KEGGihsa:10695.
UCSCiuc003ota.5. human. [Q9BT09-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358960 mRNA. Translation: AAQ89319.1.
AL035587 Genomic DNA. Translation: CAB75301.1.
BC004423 mRNA. Translation: AAH04423.1.
BC008133 mRNA. No translation available.
BC008898 mRNA. Translation: AAH08898.1.
BC008961 mRNA. Translation: AAH08961.1.
BC022093 mRNA. Translation: AAH22093.3.
U80744 mRNA. Translation: AAB91442.1. Frameshift.
AF161347 mRNA. Translation: AAF28907.1. Frameshift.
AK090425 mRNA. Translation: BAC03406.1.
CCDSiCCDS4875.1. [Q9BT09-1]
RefSeqiNP_006577.2. NM_006586.4. [Q9BT09-1]
UniGeneiHs.414099.

3D structure databases

ProteinModelPortaliQ9BT09.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115934. 25 interactions.
IntActiQ9BT09. 3 interactions.
STRINGi9606.ENSP00000361926.

PTM databases

iPTMnetiQ9BT09.
PhosphoSiteiQ9BT09.

Polymorphism and mutation databases

BioMutaiCNPY3.
DMDMi74752319.

Proteomic databases

EPDiQ9BT09.
MaxQBiQ9BT09.
PaxDbiQ9BT09.
PRIDEiQ9BT09.

Protocols and materials databases

DNASUi10695.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372836; ENSP00000361926; ENSG00000137161. [Q9BT09-1]
GeneIDi10695.
KEGGihsa:10695.
UCSCiuc003ota.5. human. [Q9BT09-1]

Organism-specific databases

CTDi10695.
GeneCardsiCNPY3.
H-InvDBHIX0005887.
HGNCiHGNC:11968. CNPY3.
HPAiHPA016560.
MIMi610774. gene.
neXtProtiNX_Q9BT09.
PharmGKBiPA162382601.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4052. Eukaryota.
ENOG4111GVI. LUCA.
GeneTreeiENSGT00390000014072.
HOGENOMiHOG000030912.
HOVERGENiHBG107736.
InParanoidiQ9BT09.
OMAiDLTQFLC.
OrthoDBiEOG7SBNPF.
PhylomeDBiQ9BT09.
TreeFamiTF318951.

Enzyme and pathway databases

ReactomeiR-HSA-1679131. Trafficking and processing of endosomal TLR.

Miscellaneous databases

ChiTaRSiCNPY3. human.
GenomeRNAii10695.
NextBioi40653.
PROiQ9BT09.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BT09.
CleanExiHS_CNPY3.
ExpressionAtlasiQ9BT09. baseline and differential.
GenevisibleiQ9BT09. HS.

Family and domain databases

InterProiIPR021852. DUF3456.
[Graphical view]
PfamiPF11938. DUF3456. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  2. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cervix, Colon and Lung.
  4. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-192 (ISOFORM 1).
    Tissue: Brain cortex.
  5. "Human partial CDS from CD34+ stem cells."
    Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L., Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 153-278 (ISOFORM 1).
    Tissue: Umbilical cord blood.
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 165-278 (ISOFORM 1).
    Tissue: Spleen.
  7. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-153.
    Tissue: Liver.
  8. "Folding of Toll-like receptors by the HSP90 paralogue gp96 requires a substrate-specific cochaperone."
    Liu B., Yang Y., Qiu Z., Staron M., Hong F., Li Y., Wu S., Li Y., Hao B., Bona R., Han D., Li Z.
    Nat. Commun. 1:79-79(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HSP90B1.
  9. Erratum
    Liu B., Yang Y., Qiu Z., Staron M., Hong F., Li Y., Wu S., Li Y., Hao B., Bona R., Han D., Li Z.
    Nat. Commun. 3:653-653(2012)
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCNPY3_HUMAN
AccessioniPrimary (citable) accession number: Q9BT09
Secondary accession number(s): O15412
, Q0P6I2, Q8NF54, Q8WTU8, Q9P0F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2001
Last modified: April 13, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.