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Q9BSW2

- EFC4B_HUMAN

UniProt

Q9BSW2 - EFC4B_HUMAN

Protein

EF-hand calcium-binding domain-containing protein 4B

Gene

CRACR2A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Ca2+-binding protein that plays a key role in store-operated Ca2+ entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca2+ resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi97 – 10812Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. activation of store-operated calcium channel activity Source: UniProtKB
    2. immune system process Source: UniProtKB-KW
    3. positive regulation of calcium ion transport Source: UniProtKB
    4. store-operated calcium entry Source: UniProtKB

    Keywords - Biological processi

    Adaptive immunity, Calcium transport, Immunity, Ion transport, Transport

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EF-hand calcium-binding domain-containing protein 4B
    Alternative name(s):
    Calcium release-activated calcium channel regulator 2A
    Short name:
    CRAC channel regulator 2A
    Calcium release-activated channel regulator 2A
    Gene namesi
    Name:CRACR2A
    Synonyms:EFCAB4B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:28657. CRACR2A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. Golgi apparatus Source: HPA
    3. membrane Source: UniProtKB
    4. nucleolus Source: HPA
    5. nucleus Source: HPA

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi97 – 993DAD → AAA in EF2MUT; enhanced STIM1 clustering and elevated cytoplasmic Ca2+, thereby causing cell death in T-cells. 1 Publication

    Organism-specific databases

    PharmGKBiPA144596438.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395EF-hand calcium-binding domain-containing protein 4BPRO_0000283046Add
    BLAST

    Proteomic databases

    MaxQBiQ9BSW2.
    PaxDbiQ9BSW2.
    PRIDEiQ9BSW2.

    PTM databases

    PhosphoSiteiQ9BSW2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9BSW2.
    BgeeiQ9BSW2.
    CleanExiHS_EFCAB4B.
    GenevestigatoriQ9BSW2.

    Organism-specific databases

    HPAiHPA038686.

    Interactioni

    Subunit structurei

    Interacts with ORAI1 and STIM1; the interaction is direct and takes place in absence of Ca2+. Forms a complex with ORAI1 and STIM1 at low concentration of Ca2+, the complex dissociates at elevated Ca2+ concentrations. Interacts with ORAI2 and ORAI3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Hoxa1P090223EBI-739773,EBI-3957603From a different organism.
    ORAI1Q96D316EBI-739773,EBI-2291476
    STIM1Q135863EBI-739773,EBI-448878

    Protein-protein interaction databases

    BioGridi124247. 4 interactions.
    IntActiQ9BSW2. 7 interactions.
    MINTiMINT-1455075.
    STRINGi9606.ENSP00000409382.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BSW2.
    SMRiQ9BSW2. Positions 55-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 8229EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini84 – 11936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili201 – 373173Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the EFCAB4 family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000112335.
    HOVERGENiHBG107143.
    InParanoidiQ9BSW2.
    KOiK17199.
    OMAiEQPLSKC.
    OrthoDBiEOG7NW68N.
    PhylomeDBiQ9BSW2.
    TreeFamiTF329556.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9BSW2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAPDGRVVS RPQRLGQGSG QGPKGSGACL HPLDSLEQKE TQEQTSGQLV    50
    MLRKAQEFFQ TCDAEGKGFI ARKDMQRLHK ELPLSLEELE DVFDALDADG 100
    NGYLTPQEFT TGFSHFFFSQ NNPSQEDAGE QVAQRHEEKV YLSRGDEDLG 150
    DMGEDEEAQF RMLMDRLGAQ KVLEDESDVK QLWLQLKKEE PHLLSNFEDF 200
    LTRIISQLQE AHEEKNELEC ALKRKIAAYD EEIQHLYEEM EQQIKSEKEQ 250
    FLLKDTERFQ ARSQELEQKL LCKEQELEQL TQKQKRLEGQ CTALHHDKHE 300
    TKAENTKLKL TNQELARELE RTSWELQDAQ QQLESLQQEA CKLHQEKEME 350
    VYRVTESLQR EKAGLLKQLD FLRCVGGHWP VLRAPPRSLG SEGPV 395
    Length:395
    Mass (Da):45,592
    Last modified:June 1, 2001 - v1
    Checksum:i9A69FA0D8B769860
    GO
    Isoform 2 (identifier: Q9BSW2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         374-395: CVGGHWPVLRAPPRSLGSEGPV → ERNKHLRDER...HPAKKKSCCG

    Note: No experimental confirmation available.

    Show »
    Length:731
    Mass (Da):83,193
    Checksum:iDE746B5B604ACC44
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti7 – 71R → G.
    Corresponds to variant rs9788233 [ dbSNP | Ensembl ].
    VAR_031483
    Natural varianti98 – 981A → T.
    Corresponds to variant rs17836273 [ dbSNP | Ensembl ].
    VAR_031484
    Natural varianti128 – 1281A → V.1 Publication
    Corresponds to variant rs242017 [ dbSNP | Ensembl ].
    VAR_031485
    Natural varianti136 – 1361H → P.
    Corresponds to variant rs34088152 [ dbSNP | Ensembl ].
    VAR_031486
    Natural varianti154 – 1541E → K.1 Publication
    Corresponds to variant rs242018 [ dbSNP | Ensembl ].
    VAR_031487
    Natural varianti212 – 2121H → Q.
    Corresponds to variant rs36030417 [ dbSNP | Ensembl ].
    VAR_031488

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei374 – 39522CVGGH…SEGPV → ERNKHLRDERDICFQKNKAA KANTAASRASWKKRSGSVIG KYVDSRGILRSQSEEEEEVF GIPRRSSLGLSGYPLTEEEP GTGEPGPGGPYPRPLRRIIS VEEDPLPQLLDGGFEQPLSK CSEEEEVSDQGVQGQIPEAP PLKLTPTSPRGQPVGKEALC KEESSPSAPDRLFKIVFVGN SAVGKTSFLRRFCEDRFSPG MAATVGIDYRVKTLNVDNSQ VALQLWDTAGQERYRCITQQ FFRKADGVIVMYDLTDKQSF LSVRRWLSSVEEAVGDRVPV LLLGNKLDNEKEREVPRGLG EQLATENNLIFYECSAYSGH NTKESLLHLARFLKEQEDTV REDTIQVGHPAKKKSCCG in isoform 2. 1 PublicationVSP_039546Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK304017 mRNA. Translation: BAG64932.1.
    AC005831 Genomic DNA. No translation available.
    AC006207 Genomic DNA. No translation available.
    BC004524 mRNA. Translation: AAH04524.1.
    BC150643 mRNA. Translation: AAI50644.1.
    CCDSiCCDS44803.1. [Q9BSW2-2]
    CCDS8522.1. [Q9BSW2-1]
    RefSeqiNP_001138430.1. NM_001144958.1. [Q9BSW2-2]
    NP_116069.1. NM_032680.3. [Q9BSW2-1]
    UniGeneiHs.504534.

    Genome annotation databases

    EnsembliENST00000252322; ENSP00000252322; ENSG00000130038. [Q9BSW2-1]
    ENST00000440314; ENSP00000409382; ENSG00000130038. [Q9BSW2-2]
    GeneIDi84766.
    KEGGihsa:84766.
    UCSCiuc001qmj.2. human. [Q9BSW2-1]
    uc010sen.1. human. [Q9BSW2-2]

    Polymorphism databases

    DMDMi74761240.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK304017 mRNA. Translation: BAG64932.1 .
    AC005831 Genomic DNA. No translation available.
    AC006207 Genomic DNA. No translation available.
    BC004524 mRNA. Translation: AAH04524.1 .
    BC150643 mRNA. Translation: AAI50644.1 .
    CCDSi CCDS44803.1. [Q9BSW2-2 ]
    CCDS8522.1. [Q9BSW2-1 ]
    RefSeqi NP_001138430.1. NM_001144958.1. [Q9BSW2-2 ]
    NP_116069.1. NM_032680.3. [Q9BSW2-1 ]
    UniGenei Hs.504534.

    3D structure databases

    ProteinModelPortali Q9BSW2.
    SMRi Q9BSW2. Positions 55-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124247. 4 interactions.
    IntActi Q9BSW2. 7 interactions.
    MINTi MINT-1455075.
    STRINGi 9606.ENSP00000409382.

    PTM databases

    PhosphoSitei Q9BSW2.

    Polymorphism databases

    DMDMi 74761240.

    Proteomic databases

    MaxQBi Q9BSW2.
    PaxDbi Q9BSW2.
    PRIDEi Q9BSW2.

    Protocols and materials databases

    DNASUi 84766.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000252322 ; ENSP00000252322 ; ENSG00000130038 . [Q9BSW2-1 ]
    ENST00000440314 ; ENSP00000409382 ; ENSG00000130038 . [Q9BSW2-2 ]
    GeneIDi 84766.
    KEGGi hsa:84766.
    UCSCi uc001qmj.2. human. [Q9BSW2-1 ]
    uc010sen.1. human. [Q9BSW2-2 ]

    Organism-specific databases

    CTDi 84766.
    GeneCardsi GC12M003725.
    HGNCi HGNC:28657. CRACR2A.
    HPAi HPA038686.
    MIMi 614178. gene.
    neXtProti NX_Q9BSW2.
    PharmGKBi PA144596438.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000112335.
    HOVERGENi HBG107143.
    InParanoidi Q9BSW2.
    KOi K17199.
    OMAi EQPLSKC.
    OrthoDBi EOG7NW68N.
    PhylomeDBi Q9BSW2.
    TreeFami TF329556.

    Miscellaneous databases

    GenomeRNAii 84766.
    NextBioi 74911.
    PROi Q9BSW2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BSW2.
    Bgeei Q9BSW2.
    CleanExi HS_EFCAB4B.
    Genevestigatori Q9BSW2.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Trachea.
    2. "The finished DNA sequence of human chromosome 12."
      Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
      , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
      Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-128 AND LYS-154.
      Tissue: Brain and Muscle.
    4. "A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells."
      Srikanth S., Jung H.J., Kim K.D., Souda P., Whitelegge J., Gwack Y.
      Nat. Cell Biol. 12:436-446(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, CALCIUM-BINDING, INTERACTION WITH ORAI1; ORAI2; ORAI3 AND STIM1, MUTAGENESIS OF 97-ASP--ASP-99.

    Entry informationi

    Entry nameiEFC4B_HUMAN
    AccessioniPrimary (citable) accession number: Q9BSW2
    Secondary accession number(s): B4E1X0, B9EK63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3