Rhotekin - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot Q9BST9 (RTKN_HUMAN)

Last modified February 9, 2010. Version 69. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Rhotekin

Gene names

Name:	RTKN
Synonyms:	RTKN1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. Ref.3 Ref.4 Ref.5
Subunit structure	Interacts via its C-terminal region with the TAX1BP3 PDZ domain. This interaction facilitates Rho-mediated activation of the c-Fos serum response element (SRE). Interacts with SEPT9. Specifically binds to GTP-bound RHOA, RHOB and RHOC and inhibits their GTPase activity. Ref.3 Ref.5 UniProtKB Q8C6B2
Tissue specificity	Highly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines. Ref.4 Ref.1
Sequence similarities	Contains 1 PH domain. Contains 1 REM (Hr1) repeat.
Sequence caution	The sequence AAL16767.1 differs from that shown. Reason: Frameshift at positions 15 and 20.

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Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Rho protein signal transduction Ref.4 Inferred from direct assay. Source: UniProtKB apoptosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of anti-apoptosis Ref.4 Inferred from direct assay. Source: UniProtKB
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP-Rho binding Ref.3 Inferred from direct assay. Source: UniProtKB GTPase inhibitor activity Ref.3 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
RHOA	P61586	1	EBI-446694,EBI-446668
Rhoa	Q9QUI0	1	EBI-446694,EBI-643583	From a different organism.

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q9BST9-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: Q9BST9-2) The sequence of this isoform differs from the canonical sequence as follows: 1-36: MFSRNHRSRVTVARGSALEMEFKRGRFRLSLFSDLP → MQDRLHILEDLNMLYIRQMALSL
Note: No experimental confirmation is available.

Isoform 3 Ref.3 (identifier: Q9BST9-3) The sequence of this isoform differs from the canonical sequence as follows: 1-36: MFSRNHRSRV...FRLSLFSDLP → SESGLVSCLE...LSLSSLLWKS
Note: Incomplete sequence.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 563

563

Rhotekin

PRO_0000233940

Regions

Repeat

36 – 99

REM

Domain

309 – 416

108

Compositional bias

479 – 544

Pro-rich

Amino acid modifications

Modified residue

106

Phosphoserine

Modified residue

232

Phosphoserine Ref.6

Modified residue

241

Phosphoserine Ref.6

Modified residue

520

Phosphoserine By similarity

Modified residue

543

Phosphoserine Ref.6

Natural variations

Alternative sequence

1 – 36

MFSRN…FSDLP → MQDRLHILEDLNMLYIRQMA LSL in isoform 2. Ref.2

VSP_052004

Alternative sequence

1 – 36

MFSRN…FSDLP → SESGLVSCLEGKGPAEGSGD SSRQAHPCLPLSLSSLLWKS in isoform 3. Ref.3

VSP_052005

Experimental info

Mutagenesis

561 – 563

SPV → APA: Impairs interaction with TAX1BP3. Ref.3

Sequence conflict

18 – 19

LE → WR Ref.1

Sequence conflict

A → P in AAL16767. Ref.1

Sequence conflict

A → P in AAL16767. Ref.1

Sequence conflict

65 – 66

RE → AR in AAL16767. Ref.1

Sequence conflict

102

S → N in AAL16767. Ref.1

Sequence conflict

112

A → P in AAL16767. Ref.1

Sequence conflict

160

Q → H in AAG01181. Ref.3

Sequence conflict

163

E → D in AAG01181. Ref.3

Sequence conflict

178

S → N in AAG01181. Ref.3

Sequence conflict

202 – 205

EEGA → KKRG in AAG01181. Ref.3

Sequence conflict

227

R → K in AAG01181. Ref.3

Sequence conflict

274

R → L in AAL16767. Ref.1

Sequence conflict

336

G → S in AAG01181. Ref.3

Sequence conflict

356

L → F in AAL16767. Ref.1

Sequence conflict

365 – 366

RV → PI in AAG01181. Ref.3

Sequence conflict

372

D → E in AAG01181. Ref.3

Sequence conflict

401 – 406

REALQS → LETLQN in AAG01181. Ref.3

Sequence conflict

437 – 439

RKP → PKT in AAG01181. Ref.3

Sequence conflict

454

A → V in AAG01181. Ref.3

Sequence conflict

540

P → T in AAG01181. Ref.3

Sequence conflict

549

L → F in AAG01181. Ref.3

Sequence conflict

555

P → L in AAG01181. Ref.3

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: B3C64CB737E88501 Show »	FASTA	563	62,667
10 20 30 40 50 60 MFSRNHRSRV TVARGSALEM EFKRGRFRLS LFSDLPEDTE LQRKLDHEIR MREGACKLLA 70 80 90 100 110 120 ACSQREQALE ATKSLLVCNS RILSYMGELQ RRKEAQVLGK TSRRPSDSGP PAERSPCRGR 130 140 150 160 170 180 VCISDLRIPL MWKDTEYFKN KGDLHRWAVF LLLQLGEHIQ DTEMILVDRT LTDISFQSNV 190 200 210 220 230 240 LFAEAGPDFE LRLELYGACV EEEGALTGGP KRLATKLSSS LGRSSGRRVR ASLDSAGGSG 250 260 270 280 290 300 SSPILLPTPV VGGPRYHLLA HTTLTLAAVQ DGFRTHDLTL ASHEENPAWL PLYGSVCCRL 310 320 330 340 350 360 AAQPLCMTQP TASGTLRVQQ AGEMQNWAQV HGVLKGTNLF CYRQPEDADT GEEPLLTIAV 370 380 390 400 410 420 NKETRVRAGE LDQALGRPFT LSISNQYGDD EVTHTLQTES REALQSWMEA LWQLFFDMSQ 430 440 450 460 470 480 WKQCCDEIMK IETPAPRKPP QALAKQGSLY HEMAIEPLDD IAAVTDILTQ REGARLETPP 490 500 510 520 530 540 PWLAMFTDQP ALPNPCSPAS VAPAPDWTHP LPWGRPRTFS LDAVPPDHSP RARSVAPLPP 550 560 QRSPRTRGLC SKGQPRTWLQ SPV « Hide
Isoform 2. Checksum: 606C424DF55DD690 Show »	FASTA	550	61,229
10 20 30 40 50 60 MQDRLHILED LNMLYIRQMA LSLEDTELQR KLDHEIRMRE GACKLLAACS QREQALEATK 70 80 90 100 110 120 SLLVCNSRIL SYMGELQRRK EAQVLGKTSR RPSDSGPPAE RSPCRGRVCI SDLRIPLMWK 130 140 150 160 170 180 DTEYFKNKGD LHRWAVFLLL QLGEHIQDTE MILVDRTLTD ISFQSNVLFA EAGPDFELRL 190 200 210 220 230 240 ELYGACVEEE GALTGGPKRL ATKLSSSLGR SSGRRVRASL DSAGGSGSSP ILLPTPVVGG 250 260 270 280 290 300 PRYHLLAHTT LTLAAVQDGF RTHDLTLASH EENPAWLPLY GSVCCRLAAQ PLCMTQPTAS 310 320 330 340 350 360 GTLRVQQAGE MQNWAQVHGV LKGTNLFCYR QPEDADTGEE PLLTIAVNKE TRVRAGELDQ 370 380 390 400 410 420 ALGRPFTLSI SNQYGDDEVT HTLQTESREA LQSWMEALWQ LFFDMSQWKQ CCDEIMKIET 430 440 450 460 470 480 PAPRKPPQAL AKQGSLYHEM AIEPLDDIAA VTDILTQREG ARLETPPPWL AMFTDQPALP 490 500 510 520 530 540 NPCSPASVAP APDWTHPLPW GRPRTFSLDA VPPDHSPRAR SVAPLPPQRS PRTRGLCSKG 550 QPRTWLQSPV « Hide
Isoform 3. Checksum: 0EC051729C332E05 Show »	FASTA	567	62,483
10 20 30 40 50 60 SESGLVSCLE GKGPAEGSGD SSRQAHPCLP LSLSSLLWKS EDTELQRKLD HEIRMREGAC 70 80 90 100 110 120 KLLAACSQRE QALEATKSLL VCNSRILSYM GELQRRKEAQ VLGKTSRRPS DSGPPAERSP 130 140 150 160 170 180 CRGRVCISDL RIPLMWKDTE YFKNKGDLHR WAVFLLLQLG EHIQDTEMIL VDRTLTDISF 190 200 210 220 230 240 QSNVLFAEAG PDFELRLELY GACVEEEGAL TGGPKRLATK LSSSLGRSSG RRVRASLDSA 250 260 270 280 290 300 GGSGSSPILL PTPVVGGPRY HLLAHTTLTL AAVQDGFRTH DLTLASHEEN PAWLPLYGSV 310 320 330 340 350 360 CCRLAAQPLC MTQPTASGTL RVQQAGEMQN WAQVHGVLKG TNLFCYRQPE DADTGEEPLL 370 380 390 400 410 420 TIAVNKETRV RAGELDQALG RPFTLSISNQ YGDDEVTHTL QTESREALQS WMEALWQLFF 430 440 450 460 470 480 DMSQWKQCCD EIMKIETPAP RKPPQALAKQ GSLYHEMAIE PLDDIAAVTD ILTQREGARL 490 500 510 520 530 540 ETPPPWLAMF TDQPALPNPC SPASVAPAPD WTHPLPWGRP RTFSLDAVPP DHSPRARSVA 550 560 PLPPQRSPRT RGLCSKGQPR TWLQSPV « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, expression characterization, and mapping of a novel putative inhibitor of rho GTPase activity, RTKN, to D2S145-D2S286." Fu Q., Yu L., Liu Q., Zhang J., Zhang H., Zhao S. Genomics 66:328-332(2000) [PubMed: 10873388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Placenta.
[3]	"The PDZ protein TIP-1 interacts with the Rho effector rhotekin and is involved in Rho signaling to the serum response element." Reynaud C., Fabre S., Jalinot P. J. Biol. Chem. 275:33962-33968(2000) [PubMed: 10940294] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, INTERACTION WITH RHOA AND TAX1BP3, MUTAGENESIS OF 561-SER--VAL-563. Tissue: Leukocyte.
[4]	"Rho/Rhotekin-mediated NF-kappaB activation confers resistance to apoptosis." Liu C.-A., Wang M.-J., Chi C.-W., Wu C.-W., Chen J.-Y. Oncogene 23:8731-8742(2004) [PubMed: 15480428] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"Possible role of Rho/Rhotekin signaling in mammalian septin organization." Ito H., Iwamoto I., Morishita R., Nozawa Y., Narumiya S., Asano T., Nagata K. Oncogene 24:7064-7072(2005) [PubMed: 16007136] [Abstract] Cited for: FUNCTION, INTERACTION WITH SEPT9.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-241 AND SER-543, MASS SPECTROMETRY.
[7]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AF049227 mRNA. Translation: AAL16767.1. Frameshift. BC004558 mRNA. Translation: AAH04558.2. BC017727 mRNA. Translation: AAH17727.1. AF290512 mRNA. Translation: AAG01181.1.
IPI	IPI00029834. IPI00157230. IPI00386977.
RefSeq	NP_001015055.1. NP_001015056.1. NP_149035.1.
UniGene	Hs.192854
3D structure databases
SMR	Q9BST9. Positions 322-412.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BST9. 2 interactions.
STRING	Q9BST9.
PTM databases
PhosphoSite	Q9BST9.
Proteomic databases
PRIDE	Q9BST9.
Genome annotation databases
Ensembl	ENST00000272430; ENSP00000272430; ENSG00000114993; Homo sapiens. [Genome view]
GeneID	6242.
KEGG	hsa:6242.
UCSC	uc002sle.1. human.
Organism-specific databases
CTD	6242.
GeneCards	GC02M074506.
H-InvDB	HIX0002182.
HGNC	HGNC:10466. RTKN.
MIM	602288. gene.
PharmGKB	PA34879.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04291.
HOGENOM	HBG715547.
HOVERGEN	Q9BST9.
InParanoid	Q9BST9.
OMA	HEMAIEP.
OrthoDB	EOG96X1V2.
Gene expression databases
ArrayExpress	Q9BST9.
Bgee	Q9BST9.
CleanEx	HS_RTKN.
Genevestigator	Q9BST9.
GermOnline	ENSG00000114993. Homo sapiens.
Family and domain databases
InterPro	IPR000861. HR1-like_rho-bd. IPR011072. HR1_rho-bd. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF02185. HR1. 1 hit. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00742. Hr1. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	24241.
SOURCE	Search...

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Entry information

Entry name

RTKN_HUMAN

Accession

Primary (citable) accession number: Q9BST9
Secondary accession number(s): Q8WVN1, Q96PT6, Q9HB05

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	March 1, 2004
Last modified:	February 9, 2010
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents