Q9BST9 (RTKN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhotekin

Gene names

Name:	RTKN
Synonyms:	RTKN1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. Ref.3 Ref.4 Ref.5
Subunit structure	Interacts via its C-terminal region with the TAX1BP3 PDZ domain. This interaction facilitates Rho-mediated activation of the c-Fos serum response element (SRE). Interacts with SEPT9. Specifically binds to GTP-bound RHOA, RHOB and RHOC and inhibits their GTPase activity. Ref.3 Ref.5 UniProtKB Q8C6B2
Tissue specificity	Highly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines. Ref.1 Ref.4
Sequence similarities	Contains 1 PH domain. Contains 1 REM (Hr1) repeat.
Sequence caution	The sequence AAL16767.1 differs from that shown. Reason: Frameshift at positions 15 and 20.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Inferred from direct assay Ref.4. Source: UniProtKB apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP-Rho binding Inferred from direct assay Ref.3. Source: UniProtKB GTPase inhibitor activity Inferred from direct assay Ref.3. Source: UniProtKB phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q9BST9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 (identifier: Q9BST9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MFSRNHRSRVTVARGSALEMEFKRGRFRLSLFSDLP → MQDRLHILEDLNMLYIRQMALSL

Note: No experimental confirmation available.

Isoform 3 Ref.3 (identifier: Q9BST9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MFSRNHRSRV...FRLSLFSDLP → SESGLVSCLE...LSLSSLLWKS

Note: Incomplete sequence.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	Rhotekin		PRO_0000233940
Regions
	Repeat	36 – 99	64	REM
	Domain	309 – 416	108	PH
	Compositional bias	479 – 544	66	Pro-rich
Amino acid modifications
	Modified residue	106	1	Phosphoserine By similarity
	Modified residue	232	1	Phosphoserine Ref.6 Ref.7
	Modified residue	520	1	Phosphoserine By similarity
	Modified residue	543	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 36	36	MFSRN…FSDLP → MQDRLHILEDLNMLYIRQMA LSL in isoform 2. Ref.2		VSP_052004
	Alternative sequence	1 – 36	36	MFSRN…FSDLP → SESGLVSCLEGKGPAEGSGD SSRQAHPCLPLSLSSLLWKS in isoform 3. Ref.3		VSP_052005
Experimental info
	Mutagenesis	561 – 563	3	SPV → APA: Impairs interaction with TAX1BP3. Ref.3
	Sequence conflict	18 – 19	2	LE → WR Ref.1
	Sequence conflict	55	1	A → P in AAL16767. Ref.1
	Sequence conflict	61	1	A → P in AAL16767. Ref.1
	Sequence conflict	65 – 66	2	RE → AR in AAL16767. Ref.1
	Sequence conflict	102	1	S → N in AAL16767. Ref.1
	Sequence conflict	112	1	A → P in AAL16767. Ref.1
	Sequence conflict	160	1	Q → H in AAG01181. Ref.3
	Sequence conflict	163	1	E → D in AAG01181. Ref.3
	Sequence conflict	178	1	S → N in AAG01181. Ref.3
	Sequence conflict	202 – 205	4	EEGA → KKRG in AAG01181. Ref.3
	Sequence conflict	227	1	R → K in AAG01181. Ref.3
	Sequence conflict	274	1	R → L in AAL16767. Ref.1
	Sequence conflict	336	1	G → S in AAG01181. Ref.3
	Sequence conflict	356	1	L → F in AAL16767. Ref.1
	Sequence conflict	365 – 366	2	RV → PI in AAG01181. Ref.3
	Sequence conflict	372	1	D → E in AAG01181. Ref.3
	Sequence conflict	401 – 406	6	REALQS → LETLQN in AAG01181. Ref.3
	Sequence conflict	437 – 439	3	RKP → PKT in AAG01181. Ref.3
	Sequence conflict	454	1	A → V in AAG01181. Ref.3
	Sequence conflict	540	1	P → T in AAG01181. Ref.3
	Sequence conflict	549	1	L → F in AAG01181. Ref.3
	Sequence conflict	555	1	P → L in AAG01181. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: B3C64CB737E88501	FASTA	563	62,667
	10 20 30 40 50 60 MFSRNHRSRV TVARGSALEM EFKRGRFRLS LFSDLPEDTE LQRKLDHEIR MREGACKLLA 70 80 90 100 110 120 ACSQREQALE ATKSLLVCNS RILSYMGELQ RRKEAQVLGK TSRRPSDSGP PAERSPCRGR 130 140 150 160 170 180 VCISDLRIPL MWKDTEYFKN KGDLHRWAVF LLLQLGEHIQ DTEMILVDRT LTDISFQSNV 190 200 210 220 230 240 LFAEAGPDFE LRLELYGACV EEEGALTGGP KRLATKLSSS LGRSSGRRVR ASLDSAGGSG 250 260 270 280 290 300 SSPILLPTPV VGGPRYHLLA HTTLTLAAVQ DGFRTHDLTL ASHEENPAWL PLYGSVCCRL 310 320 330 340 350 360 AAQPLCMTQP TASGTLRVQQ AGEMQNWAQV HGVLKGTNLF CYRQPEDADT GEEPLLTIAV 370 380 390 400 410 420 NKETRVRAGE LDQALGRPFT LSISNQYGDD EVTHTLQTES REALQSWMEA LWQLFFDMSQ 430 440 450 460 470 480 WKQCCDEIMK IETPAPRKPP QALAKQGSLY HEMAIEPLDD IAAVTDILTQ REGARLETPP 490 500 510 520 530 540 PWLAMFTDQP ALPNPCSPAS VAPAPDWTHP LPWGRPRTFS LDAVPPDHSP RARSVAPLPP 550 560 QRSPRTRGLC SKGQPRTWLQ SPV
	Isoform 2 [UniParc]. Checksum: 606C424DF55DD690	FASTA	550	61,229
	10 20 30 40 50 60 MQDRLHILED LNMLYIRQMA LSLEDTELQR KLDHEIRMRE GACKLLAACS QREQALEATK 70 80 90 100 110 120 SLLVCNSRIL SYMGELQRRK EAQVLGKTSR RPSDSGPPAE RSPCRGRVCI SDLRIPLMWK 130 140 150 160 170 180 DTEYFKNKGD LHRWAVFLLL QLGEHIQDTE MILVDRTLTD ISFQSNVLFA EAGPDFELRL 190 200 210 220 230 240 ELYGACVEEE GALTGGPKRL ATKLSSSLGR SSGRRVRASL DSAGGSGSSP ILLPTPVVGG 250 260 270 280 290 300 PRYHLLAHTT LTLAAVQDGF RTHDLTLASH EENPAWLPLY GSVCCRLAAQ PLCMTQPTAS 310 320 330 340 350 360 GTLRVQQAGE MQNWAQVHGV LKGTNLFCYR QPEDADTGEE PLLTIAVNKE TRVRAGELDQ 370 380 390 400 410 420 ALGRPFTLSI SNQYGDDEVT HTLQTESREA LQSWMEALWQ LFFDMSQWKQ CCDEIMKIET 430 440 450 460 470 480 PAPRKPPQAL AKQGSLYHEM AIEPLDDIAA VTDILTQREG ARLETPPPWL AMFTDQPALP 490 500 510 520 530 540 NPCSPASVAP APDWTHPLPW GRPRTFSLDA VPPDHSPRAR SVAPLPPQRS PRTRGLCSKG 550 QPRTWLQSPV
	Isoform 3 [UniParc]. Checksum: 0EC051729C332E05	FASTA	567	62,483
	10 20 30 40 50 60 SESGLVSCLE GKGPAEGSGD SSRQAHPCLP LSLSSLLWKS EDTELQRKLD HEIRMREGAC 70 80 90 100 110 120 KLLAACSQRE QALEATKSLL VCNSRILSYM GELQRRKEAQ VLGKTSRRPS DSGPPAERSP 130 140 150 160 170 180 CRGRVCISDL RIPLMWKDTE YFKNKGDLHR WAVFLLLQLG EHIQDTEMIL VDRTLTDISF 190 200 210 220 230 240 QSNVLFAEAG PDFELRLELY GACVEEEGAL TGGPKRLATK LSSSLGRSSG RRVRASLDSA 250 260 270 280 290 300 GGSGSSPILL PTPVVGGPRY HLLAHTTLTL AAVQDGFRTH DLTLASHEEN PAWLPLYGSV 310 320 330 340 350 360 CCRLAAQPLC MTQPTASGTL RVQQAGEMQN WAQVHGVLKG TNLFCYRQPE DADTGEEPLL 370 380 390 400 410 420 TIAVNKETRV RAGELDQALG RPFTLSISNQ YGDDEVTHTL QTESREALQS WMEALWQLFF 430 440 450 460 470 480 DMSQWKQCCD EIMKIETPAP RKPPQALAKQ GSLYHEMAIE PLDDIAAVTD ILTQREGARL 490 500 510 520 530 540 ETPPPWLAMF TDQPALPNPC SPASVAPAPD WTHPLPWGRP RTFSLDAVPP DHSPRARSVA 550 560 PLPPQRSPRT RGLCSKGQPR TWLQSPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, expression characterization, and mapping of a novel putative inhibitor of rho GTPase activity, RTKN, to D2S145-D2S286." Fu Q., Yu L., Liu Q., Zhang J., Zhang H., Zhao S. Genomics 66:328-332(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Placenta.
[3]	"The PDZ protein TIP-1 interacts with the Rho effector rhotekin and is involved in Rho signaling to the serum response element." Reynaud C., Fabre S., Jalinot P. J. Biol. Chem. 275:33962-33968(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, INTERACTION WITH RHOA AND TAX1BP3, MUTAGENESIS OF 561-SER--VAL-563. Tissue: Leukocyte.
[4]	"Rho/Rhotekin-mediated NF-kappaB activation confers resistance to apoptosis." Liu C.-A., Wang M.-J., Chi C.-W., Wu C.-W., Chen J.-Y. Oncogene 23:8731-8742(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"Possible role of Rho/Rhotekin signaling in mammalian septin organization." Ito H., Iwamoto I., Morishita R., Nozawa Y., Narumiya S., Asano T., Nagata K. Oncogene 24:7064-7072(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SEPT9.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232 AND SER-543, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF049227 mRNA. Translation: AAL16767.1. Frameshift. BC004558 mRNA. Translation: AAH04558.2. BC017727 mRNA. Translation: AAH17727.1. AF290512 mRNA. Translation: AAG01181.1.
IPI	IPI00029834. IPI00157230. IPI00386977.
RefSeq	NP_001015055.1. NM_001015055.1. NP_001015056.1. NM_001015056.1. NP_149035.1. NM_033046.2.
UniGene	Hs.192854.
3D structure databases
ProteinModelPortal	Q9BST9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BST9. 2 interactions.
MINT	MINT-142514.
STRING	9606.ENSP00000272430.
PTM databases
PhosphoSite	Q9BST9.
Polymorphism databases
DMDM	74733052.
Proteomic databases
PaxDb	Q9BST9.
PRIDE	Q9BST9.
Protocols and materials databases
DNASU	6242.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000272430; ENSP00000272430; ENSG00000114993. ENST00000305557; ENSP00000305298; ENSG00000114993.
GeneID	6242.
KEGG	hsa:6242.
UCSC	uc002slc.3. human. uc002sld.3. human.
Organism-specific databases
CTD	6242.
GeneCards	GC02M074653.
HGNC	HGNC:10466. RTKN.
HPA	HPA030259.
MIM	602288. gene.
neXtProt	NX_Q9BST9.
PharmGKB	PA34879.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG15986.
HOGENOM	HOG000015094.
HOVERGEN	HBG059480.
InParanoid	Q9BST9.
OMA	AERSPCR.
Gene expression databases
ArrayExpress	Q9BST9.
Bgee	Q9BST9.
CleanEx	HS_RTKN.
Genevestigator	Q9BST9.
GermOnline	ENSG00000114993. Homo sapiens.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR012966. DUF1709. IPR011072. HR1_rho-bd. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. [Graphical view]
Pfam	PF08174. Anillin. 1 hit. PF02185. HR1. 1 hit. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00742. Hr1. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RTKN. human.
GenomeRNAi	6242.
NextBio	24241.
SOURCE	Search...

Entry information

Entry name

RTKN_HUMAN

Accession

Primary (citable) accession number: Q9BST9
Secondary accession number(s): Q8WVN1, Q96PT6, Q9HB05

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.