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Protein

Polycomb group RING finger protein 1

Gene

PCGF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6; this transcription repressor activity may be related to PKC signaling pathway. Represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). Promotes cell cycle progression and enhances cell proliferation as well. May have a positive role in tumor cell growth by down-regulating CDKN1A. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Regulates the expression of DPPA4 and NANOG in the NT2 embryonic carcinoma cells (PubMed:26687479).4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri47 – 86RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • histone H2A monoubiquitination Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115289-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb group RING finger protein 1
Alternative name(s):
Nervous system Polycomb-1
Short name:
NSPc1
RING finger protein 68
Gene namesi
Name:PCGF1
Synonyms:NSPC1, RNF68
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:17615. PCGF1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • PcG protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi109Y → F: Marked decrease of repressor activity. May be a kinase phosphorylation site. 1 Publication1
Mutagenesisi191Y → A: Abolishes interaction with BCOR and BCORL1. 1 Publication1
Mutagenesisi193R → A: Abolishes interaction with BCOR and BCORL1. 1 Publication1
Mutagenesisi195S → F: Abolishes repressor activity. May be a PKC phosphorylation site. 1 Publication1
Mutagenesisi206V → D: Abolishes interaction with BCOR and BCORL1. 1 Publication1

Organism-specific databases

DisGeNETi84759.
OpenTargetsiENSG00000115289.
PharmGKBiPA134976631.

Polymorphism and mutation databases

BioMutaiPCGF1.
DMDMi223590124.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002778552 – 259Polycomb group RING finger protein 1Add BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei3PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BSM1.
MaxQBiQ9BSM1.
PaxDbiQ9BSM1.
PeptideAtlasiQ9BSM1.
PRIDEiQ9BSM1.

PTM databases

iPTMnetiQ9BSM1.
PhosphoSitePlusiQ9BSM1.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000115289.
CleanExiHS_PCGF1.
GenevisibleiQ9BSM1. HS.

Organism-specific databases

HPAiHPA011356.

Interactioni

Subunit structurei

Component of the repressive BCOR complex containing a Polycomb group subcomplex at least composed of RYBP, RING1 and RNF2/RING2 (PubMed:16943429). Specifically interacts with BCOR, RING1 and RNF2/RING2 (PubMed:16943429, PubMed:26687479, PubMed:23523425). Also interacts with BCORL1, the interaction is direct (PubMed:23523425). Component of a PRC1-like complex (PubMed:21282530). Interacts with CBX6, CBX7 and CBX8 (PubMed:21282530). Interacts with DPPA4, NANOG, POU5F1 and RYBP (PubMed:26687479).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCORQ6W2J98EBI-749901,EBI-950027
CBX8Q9HC524EBI-749901,EBI-712912
RING1Q065877EBI-749901,EBI-752313
RNF2Q994967EBI-749901,EBI-722416

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124243. 105 interactors.
DIPiDIP-52708N.
IntActiQ9BSM1. 27 interactors.
MINTiMINT-1460887.
STRINGi9606.ENSP00000233630.

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni158 – 161Combined sources4
Helixi163 – 165Combined sources3
Beta strandi168 – 176Combined sources9
Beta strandi191 – 195Combined sources5
Helixi200 – 211Combined sources12
Helixi215 – 217Combined sources3
Beta strandi219 – 222Combined sources4
Helixi233 – 240Combined sources8
Turni241 – 243Combined sources3
Beta strandi246 – 253Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HPLX-ray2.00B167-255[»]
4HPMX-ray1.85B/D167-255[»]
5JH5X-ray2.55C150-255[»]
ProteinModelPortaliQ9BSM1.
SMRiQ9BSM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 247Required for repressor activityAdd BLAST162
Regioni167 – 255Sufficient for interaction with BCOR and BCORL11 PublicationAdd BLAST89

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri47 – 86RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2660. Eukaryota.
ENOG410XPCN. LUCA.
GeneTreeiENSGT00550000074463.
HOGENOMiHOG000231946.
HOVERGENiHBG052826.
InParanoidiQ9BSM1.
KOiK11487.
OMAiQKFVRCS.
OrthoDBiEOG091G0EC9.
PhylomeDBiQ9BSM1.
TreeFamiTF324206.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BSM1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPQGGQIA IAMRLRNQLQ SVYKMDPLRN EEEVRVKIKD LNEHIVCCLC
60 70 80 90 100
AGYFVDATTI TECLHTFCKS CIVKYLQTSK YCPMCNIKIH ETQPLLNLKL
110 120 130 140 150
DRVMQDIVYK LVPGLQDSEE KRIREFYQSR GLDRVTQPTG EEPALSNLGL
160 170 180 190 200
PFSSFDHSKA HYYRYDEQLN LCLERLSSGK DKNKSVLQNK YVRCSVRAEV
210 220 230 240 250
RHLRRVLCHR LMLNPQHVQL LFDNEVLPDH MTMKQIWLSR WFGKPSPLLL

QYSVKEKRR
Length:259
Mass (Da):30,346
Last modified:February 10, 2009 - v2
Checksum:i456C9417E53A01B6
GO
Isoform 2 (identifier: Q9BSM1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Show »
Length:176
Mass (Da):20,904
Checksum:i18CE151AA03179E4
GO

Sequence cautioni

The sequence AAH04952 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAP97183 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0363931 – 83Missing in isoform 2. 1 PublicationAdd BLAST83

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087884 mRNA. Translation: AAP97183.1. Different initiation.
AC005041 Genomic DNA. No translation available.
BC004952 mRNA. Translation: AAH04952.1. Different initiation.
CCDSiCCDS1946.2. [Q9BSM1-1]
RefSeqiNP_116062.2. NM_032673.2. [Q9BSM1-1]
UniGeneiHs.316750.

Genome annotation databases

EnsembliENST00000233630; ENSP00000233630; ENSG00000115289. [Q9BSM1-1]
GeneIDi84759.
KEGGihsa:84759.
UCSCiuc002slz.4. human. [Q9BSM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087884 mRNA. Translation: AAP97183.1. Different initiation.
AC005041 Genomic DNA. No translation available.
BC004952 mRNA. Translation: AAH04952.1. Different initiation.
CCDSiCCDS1946.2. [Q9BSM1-1]
RefSeqiNP_116062.2. NM_032673.2. [Q9BSM1-1]
UniGeneiHs.316750.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HPLX-ray2.00B167-255[»]
4HPMX-ray1.85B/D167-255[»]
5JH5X-ray2.55C150-255[»]
ProteinModelPortaliQ9BSM1.
SMRiQ9BSM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124243. 105 interactors.
DIPiDIP-52708N.
IntActiQ9BSM1. 27 interactors.
MINTiMINT-1460887.
STRINGi9606.ENSP00000233630.

PTM databases

iPTMnetiQ9BSM1.
PhosphoSitePlusiQ9BSM1.

Polymorphism and mutation databases

BioMutaiPCGF1.
DMDMi223590124.

Proteomic databases

EPDiQ9BSM1.
MaxQBiQ9BSM1.
PaxDbiQ9BSM1.
PeptideAtlasiQ9BSM1.
PRIDEiQ9BSM1.

Protocols and materials databases

DNASUi84759.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000233630; ENSP00000233630; ENSG00000115289. [Q9BSM1-1]
GeneIDi84759.
KEGGihsa:84759.
UCSCiuc002slz.4. human. [Q9BSM1-1]

Organism-specific databases

CTDi84759.
DisGeNETi84759.
GeneCardsiPCGF1.
HGNCiHGNC:17615. PCGF1.
HPAiHPA011356.
MIMi610231. gene.
neXtProtiNX_Q9BSM1.
OpenTargetsiENSG00000115289.
PharmGKBiPA134976631.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2660. Eukaryota.
ENOG410XPCN. LUCA.
GeneTreeiENSGT00550000074463.
HOGENOMiHOG000231946.
HOVERGENiHBG052826.
InParanoidiQ9BSM1.
KOiK11487.
OMAiQKFVRCS.
OrthoDBiEOG091G0EC9.
PhylomeDBiQ9BSM1.
TreeFamiTF324206.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115289-MONOMER.

Miscellaneous databases

ChiTaRSiPCGF1. human.
GeneWikiiPCGF1.
GenomeRNAii84759.
PROiQ9BSM1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115289.
CleanExiHS_PCGF1.
GenevisibleiQ9BSM1. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCGF1_HUMAN
AccessioniPrimary (citable) accession number: Q9BSM1
Secondary accession number(s): Q7Z506
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 10, 2009
Last modified: November 30, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.