Q9BSM1 (PCGF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polycomb group RING finger protein 1

Alternative name(s):
Nervous system Polycomb-1
Short name=NSPc1
RING finger protein 68

Gene names

Name:	PCGF1
Synonyms:	NSPC1, RNF68

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6; this transcription repressor activity may be related to PKC signaling pathway. Represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). Promotes cell cycle progression and enhances cell proliferation as well. May have a positive role in tumor cell growth by down-regulating CDKN1A. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Ref.5 Ref.6 Ref.7
Subunit structure	Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, RING1 and RNF2/RING2. Specifically interacts with BCOR, RING1 and RNF2/RING2. Component of a PRC1-like complex. Interacts with CBX6, CBX7 and CBX8. Ref.6 Ref.9
Subcellular location	Nucleus Ref.5 Ref.9.
Tissue specificity	Ubiquitous. Ref.4
Sequence similarities	Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAH04952.1 differs from that shown. Reason: Erroneous initiation. The sequence AAP97183.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Repressor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H2A monoubiquitination Inferred from direct assay Ref.6. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	PcG protein complex Inferred from direct assay Ref.6Ref.9. Source: UniProtKB
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCOR	Q6W2J9	6	EBI-749901,EBI-950027
CBX8	Q9HC52	2	EBI-749901,EBI-712912
RING1	Q06587	5	EBI-749901,EBI-752313
RNF2	Q99496	4	EBI-749901,EBI-722416

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BSM1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BSM1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.8
	Chain	2 – 259	258	Polycomb group RING finger protein 1		PRO_0000277855
Regions
	Zinc finger	47 – 86	40	RING-type
	Region	13 – 115	103	No repressor activity
	Region	86 – 116	31	Necessary for repressor activity
	Region	192 – 208	17	Strong repressor activity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.8
	Modified residue	3	1	Phosphoserine Ref.8
Natural variations
	Alternative sequence	1 – 83	83	Missing in isoform 2.		VSP_036393
Experimental info
	Mutagenesis	109	1	Y → F: Marked decrease of repressor activity. May be a kinase phosphorylation site. Ref.5
	Mutagenesis	195	1	S → F: Abolishes repressor activity. May be a PKC phosphorylation site. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 456C9417E53A01B6	FASTA	259	30,346
	10 20 30 40 50 60 MASPQGGQIA IAMRLRNQLQ SVYKMDPLRN EEEVRVKIKD LNEHIVCCLC AGYFVDATTI 70 80 90 100 110 120 TECLHTFCKS CIVKYLQTSK YCPMCNIKIH ETQPLLNLKL DRVMQDIVYK LVPGLQDSEE 130 140 150 160 170 180 KRIREFYQSR GLDRVTQPTG EEPALSNLGL PFSSFDHSKA HYYRYDEQLN LCLERLSSGK 190 200 210 220 230 240 DKNKSVLQNK YVRCSVRAEV RHLRRVLCHR LMLNPQHVQL LFDNEVLPDH MTMKQIWLSR 250 WFGKPSPLLL QYSVKEKRR
	Isoform 2 [UniParc]. Checksum: 18CE151AA03179E4	FASTA	176	20,904
	10 20 30 40 50 60 MCNIKIHETQ PLLNLKLDRV MQDIVYKLVP GLQDSEEKRI REFYQSRGLD RVTQPTGEEP 70 80 90 100 110 120 ALSNLGLPFS SFDHSKAHYY RYDEQLNLCL ERLSSGKDKN KSVLQNKYVR CSVRAEVRHL 130 140 150 160 170 RRVLCHRLML NPQHVQLLFD NEVLPDHMTM KQIWLSRWFG KPSPLLLQYS VKEKRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a new human cDNA homology to human RNF3A mRNA." Ding J.B., Yu L., Chu J.H., Ge H.P., Wang X.K., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-259 (ISOFORM 1). Tissue: Uterus.
[4]	"NSPc1, a novel mammalian Polycomb gene, is expressed in neural crest-derived structures of the peripheral nervous system." Nunes M., Blanc I., Maes J., Fellous M., Robert B., McElreavey K. Mech. Dev. 102:219-222(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"NSPc1, a mainly nuclear localized protein of novel PcG family members, has a transcription repression activity related to its PKC phosphorylation site at S183." Gong Y., Wang X., Liu J., Shi L., Yin B., Peng X., Qiang B., Yuan J. FEBS Lett. 579:115-121(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, REGION, MUTAGENESIS OF TYR-109 AND SER-195.
[6]	"Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets." Gearhart M.D., Corcoran C.M., Wamstad J.A., Bardwell V.J. Mol. Cell. Biol. 26:6880-6889(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"NSPc1 is a cell growth regulator that acts as a transcriptional repressor of p21Waf1/Cip1 via the RARE element." Gong Y., Yue J., Wu X., Wang X., Wen J., Lu L., Peng X., Qiang B., Yuan J. Nucleic Acids Res. 34:6158-6169(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE. Tissue: Cervix carcinoma.
[9]	"Interaction proteomics analysis of polycomb proteins defines distinct PRC1 Complexes in mammalian cells." Vandamme J., Volkel P., Rosnoblet C., Le Faou P., Angrand P.O. Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A PRC1-LIKE COMPLEX, INTERACTION WITH CBX6; CBX7 AND CBX8, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF087884 mRNA. Translation: AAP97183.1. Different initiation. AC005041 Genomic DNA. No translation available. BC004952 mRNA. Translation: AAH04952.1. Different initiation.
IPI	IPI00902987. IPI00921560.
RefSeq	NP_116062.2. NM_032673.2.
UniGene	Hs.316750.
3D structure databases
HSSP	HSSP built from PDB template 2CKL based on UniProtKB P25916.
ProteinModelPortal	Q9BSM1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BSM1. 16 interactions.
MINT	MINT-1460887.
STRING	9606.ENSP00000233630.
PTM databases
PhosphoSite	Q9BSM1.
Polymorphism databases
DMDM	223590124.
Proteomic databases
PaxDb	Q9BSM1.
PRIDE	Q9BSM1.
Protocols and materials databases
DNASU	84759.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000233630; ENSP00000233630; ENSG00000115289.
GeneID	84759.
KEGG	hsa:84759.
UCSC	uc002sly.3. human.
Organism-specific databases
CTD	84759.
GeneCards	GC02M074732.
HGNC	HGNC:17615. PCGF1.
HPA	HPA011356.
MIM	610231. gene.
neXtProt	NX_Q9BSM1.
PharmGKB	PA134976631.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG277991.
HOGENOM	HOG000231946.
HOVERGEN	HBG052826.
InParanoid	Q9BSM1.
KO	K11487.
OMA	NEHIVCY.
OrthoDB	EOG4894N6.
Gene expression databases
Bgee	Q9BSM1.
CleanEx	HS_PCGF1.
Genevestigator	Q9BSM1.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018957. Znf_C3HC4_RING-type. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF00097. zf-C3HC4. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PCGF1. human.
GenomeRNAi	84759.
NextBio	74901.
SOURCE	Search...

Entry information

Entry name

PCGF1_HUMAN

Accession

Primary (citable) accession number: Q9BSM1
Secondary accession number(s): Q7Z506

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.