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Q9BSM1

- PCGF1_HUMAN

UniProt

Q9BSM1 - PCGF1_HUMAN

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Protein

Polycomb group RING finger protein 1

Gene

PCGF1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6; this transcription repressor activity may be related to PKC signaling pathway. Represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). Promotes cell cycle progression and enhances cell proliferation as well. May have a positive role in tumor cell growth by down-regulating CDKN1A. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri47 – 8640RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. protein C-terminus binding Source: UniProtKB
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. histone H2A monoubiquitination Source: UniProtKB
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb group RING finger protein 1
Alternative name(s):
Nervous system Polycomb-1
Short name:
NSPc1
RING finger protein 68
Gene namesi
Name:PCGF1
Synonyms:NSPC1, RNF68
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:17615. PCGF1.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. PcG protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi109 – 1091Y → F: Marked decrease of repressor activity. May be a kinase phosphorylation site. 1 Publication
Mutagenesisi191 – 1911Y → A: Abolishes interaction with BCOR and BCORL1. 1 Publication
Mutagenesisi193 – 1931R → A: Abolishes interaction with BCOR and BCORL1. 1 Publication
Mutagenesisi195 – 1951S → F: Abolishes repressor activity. May be a PKC phosphorylation site. 1 Publication
Mutagenesisi206 – 2061V → D: Abolishes interaction with BCOR and BCORL1. 1 Publication

Organism-specific databases

PharmGKBiPA134976631.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 259258Polycomb group RING finger protein 1PRO_0000277855Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei3 – 31Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9BSM1.
PaxDbiQ9BSM1.
PRIDEiQ9BSM1.

PTM databases

PhosphoSiteiQ9BSM1.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ9BSM1.
CleanExiHS_PCGF1.
GenevestigatoriQ9BSM1.

Organism-specific databases

HPAiHPA011356.

Interactioni

Subunit structurei

Component of the repressive BCOR complex containing a Polycomb group subcomplex at least composed of RYBP, RING1 and RNF2/RING2. Specifically interacts with BCOR, RING1 and RNF2/RING2. Also interacts with BCORL1, the interaction is direct. Component of a PRC1-like complex. Interacts with CBX6, CBX7 and CBX8.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCORQ6W2J96EBI-749901,EBI-950027
CBX8Q9HC522EBI-749901,EBI-712912
RING1Q065875EBI-749901,EBI-752313
RNF2Q994964EBI-749901,EBI-722416

Protein-protein interaction databases

BioGridi124243. 24 interactions.
DIPiDIP-52708N.
IntActiQ9BSM1. 16 interactions.
MINTiMINT-1460887.
STRINGi9606.ENSP00000233630.

Structurei

Secondary structure

1
259
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi168 – 1769
Beta strandi191 – 1955
Helixi200 – 21112
Helixi215 – 2173
Beta strandi219 – 2224
Helixi233 – 2408
Turni241 – 2433
Beta strandi246 – 2538

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HPLX-ray2.00B167-255[»]
4HPMX-ray1.85B/D167-255[»]
ProteinModelPortaliQ9BSM1.
SMRiQ9BSM1. Positions 35-129, 167-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 247162Required for repressor activityAdd
BLAST
Regioni167 – 25589Sufficient for interaction with BCOR and BCORL1Add
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri47 – 8640RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG277991.
GeneTreeiENSGT00550000074463.
HOGENOMiHOG000231946.
HOVERGENiHBG052826.
InParanoidiQ9BSM1.
KOiK11487.
OMAiFVRCSVR.
OrthoDBiEOG754HQ5.
PhylomeDBiQ9BSM1.
TreeFamiTF324206.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9BSM1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPQGGQIA IAMRLRNQLQ SVYKMDPLRN EEEVRVKIKD LNEHIVCCLC
60 70 80 90 100
AGYFVDATTI TECLHTFCKS CIVKYLQTSK YCPMCNIKIH ETQPLLNLKL
110 120 130 140 150
DRVMQDIVYK LVPGLQDSEE KRIREFYQSR GLDRVTQPTG EEPALSNLGL
160 170 180 190 200
PFSSFDHSKA HYYRYDEQLN LCLERLSSGK DKNKSVLQNK YVRCSVRAEV
210 220 230 240 250
RHLRRVLCHR LMLNPQHVQL LFDNEVLPDH MTMKQIWLSR WFGKPSPLLL

QYSVKEKRR
Length:259
Mass (Da):30,346
Last modified:February 10, 2009 - v2
Checksum:i456C9417E53A01B6
GO
Isoform 2 (identifier: Q9BSM1-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Show »
Length:176
Mass (Da):20,904
Checksum:i18CE151AA03179E4
GO

Sequence cautioni

The sequence AAH04952.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence AAP97183.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8383Missing in isoform 2. 1 PublicationVSP_036393Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF087884 mRNA. Translation: AAP97183.1. Different initiation.
AC005041 Genomic DNA. No translation available.
BC004952 mRNA. Translation: AAH04952.1. Different initiation.
CCDSiCCDS1946.2. [Q9BSM1-1]
RefSeqiNP_116062.2. NM_032673.2. [Q9BSM1-1]
XP_005264675.1. XM_005264618.2. [Q9BSM1-3]
UniGeneiHs.316750.

Genome annotation databases

EnsembliENST00000233630; ENSP00000233630; ENSG00000115289. [Q9BSM1-1]
GeneIDi84759.
KEGGihsa:84759.
UCSCiuc002sly.3. human. [Q9BSM1-1]

Polymorphism databases

DMDMi223590124.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF087884 mRNA. Translation: AAP97183.1 . Different initiation.
AC005041 Genomic DNA. No translation available.
BC004952 mRNA. Translation: AAH04952.1 . Different initiation.
CCDSi CCDS1946.2. [Q9BSM1-1 ]
RefSeqi NP_116062.2. NM_032673.2. [Q9BSM1-1 ]
XP_005264675.1. XM_005264618.2. [Q9BSM1-3 ]
UniGenei Hs.316750.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HPL X-ray 2.00 B 167-255 [» ]
4HPM X-ray 1.85 B/D 167-255 [» ]
ProteinModelPortali Q9BSM1.
SMRi Q9BSM1. Positions 35-129, 167-254.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124243. 24 interactions.
DIPi DIP-52708N.
IntActi Q9BSM1. 16 interactions.
MINTi MINT-1460887.
STRINGi 9606.ENSP00000233630.

PTM databases

PhosphoSitei Q9BSM1.

Polymorphism databases

DMDMi 223590124.

Proteomic databases

MaxQBi Q9BSM1.
PaxDbi Q9BSM1.
PRIDEi Q9BSM1.

Protocols and materials databases

DNASUi 84759.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000233630 ; ENSP00000233630 ; ENSG00000115289 . [Q9BSM1-1 ]
GeneIDi 84759.
KEGGi hsa:84759.
UCSCi uc002sly.3. human. [Q9BSM1-1 ]

Organism-specific databases

CTDi 84759.
GeneCardsi GC02M074732.
HGNCi HGNC:17615. PCGF1.
HPAi HPA011356.
MIMi 610231. gene.
neXtProti NX_Q9BSM1.
PharmGKBi PA134976631.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG277991.
GeneTreei ENSGT00550000074463.
HOGENOMi HOG000231946.
HOVERGENi HBG052826.
InParanoidi Q9BSM1.
KOi K11487.
OMAi FVRCSVR.
OrthoDBi EOG754HQ5.
PhylomeDBi Q9BSM1.
TreeFami TF324206.

Miscellaneous databases

ChiTaRSi PCGF1. human.
GeneWikii PCGF1.
GenomeRNAii 84759.
NextBioi 74901.
PROi Q9BSM1.
SOURCEi Search...

Gene expression databases

Bgeei Q9BSM1.
CleanExi HS_PCGF1.
Genevestigatori Q9BSM1.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view ]
Pfami PF00097. zf-C3HC4. 1 hit.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a new human cDNA homology to human RNF3A mRNA."
    Ding J.B., Yu L., Chu J.H., Ge H.P., Wang X.K., Zhao S.Y.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-259 (ISOFORM 1).
    Tissue: Uterus.
  4. "NSPc1, a novel mammalian Polycomb gene, is expressed in neural crest-derived structures of the peripheral nervous system."
    Nunes M., Blanc I., Maes J., Fellous M., Robert B., McElreavey K.
    Mech. Dev. 102:219-222(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "NSPc1, a mainly nuclear localized protein of novel PcG family members, has a transcription repression activity related to its PKC phosphorylation site at S183."
    Gong Y., Wang X., Liu J., Shi L., Yin B., Peng X., Qiang B., Yuan J.
    FEBS Lett. 579:115-121(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, REGION, MUTAGENESIS OF TYR-109 AND SER-195.
  6. "Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets."
    Gearhart M.D., Corcoran C.M., Wamstad J.A., Bardwell V.J.
    Mol. Cell. Biol. 26:6880-6889(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  7. "NSPc1 is a cell growth regulator that acts as a transcriptional repressor of p21Waf1/Cip1 via the RARE element."
    Gong Y., Yue J., Wu X., Wang X., Wen J., Lu L., Peng X., Qiang B., Yuan J.
    Nucleic Acids Res. 34:6158-6169(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Interaction proteomics analysis of polycomb proteins defines distinct PRC1 Complexes in mammalian cells."
    Vandamme J., Volkel P., Rosnoblet C., Le Faou P., Angrand P.O.
    Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A PRC1-LIKE COMPLEX, INTERACTION WITH CBX6; CBX7 AND CBX8, SUBCELLULAR LOCATION.
  10. "Structure of the polycomb group protein PCGF1 in complex with BCOR reveals basis for binding selectivity of PCGF homologs."
    Junco S.E., Wang R., Gaipa J.C., Taylor A.B., Schirf V., Gearhart M.D., Bardwell V.J., Demeler B., Hart P.J., Kim C.A.
    Structure 21:665-671(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 167-255 IN COMPLEXES WITH BCOR AND BCORL1, INTERACTION WITH BCOR AND BCORL1, MUTAGENESIS OF TYR-191; ARG-193 AND VAL-206.

Entry informationi

Entry nameiPCGF1_HUMAN
AccessioniPrimary (citable) accession number: Q9BSM1
Secondary accession number(s): Q7Z506
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 10, 2009
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3