Q9BSK4 (FEM1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein fem-1 homolog A
Short name=FEM1a

Alternative name(s):
FEM1-alpha
Prostaglandin E receptor 4-associated protein

Gene names

Name:	FEM1A
Synonyms:	EPRAP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit By similarity. May participate in antiinflammatory signaling via its interaction with PTGER4.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Component of some E3 ubiquitin-protein ligase complex By similarity. Interacts with PTGER4. Ref.1
Subcellular location	Cytoplasm Probable.
Tissue specificity	Present in macrophages derived from peripheral blood monocytes. Also present in atheromata (at protein level). Ref.1
Induction	Frequently down-regulated in rhabdomyosarcoma. Ref.7
Sequence similarities	Belongs to the fem-1 family. Contains 9 ANK repeats. Contains 2 TPR repeats.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	ANK repeat Repeat TPR repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of inflammatory response Inferred from direct assay Ref.1. Source: UniProtKB regulation of ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Protein fem-1 homolog A		PRO_0000324525
Regions
	Repeat	2 – 31	30	ANK 1
	Repeat	40 – 70	31	ANK 2
	Repeat	82 – 111	30	ANK 3
	Repeat	115 – 145	31	ANK 4
	Repeat	149 – 178	30	ANK 5
	Repeat	182 – 211	30	ANK 6
	Repeat	214 – 243	30	ANK 7
	Repeat	298 – 332	35	TPR 1
	Repeat	390 – 423	34	TPR 2
	Repeat	534 – 576	43	ANK 8
	Repeat	580 – 609	30	ANK 9
Natural variations
	Natural variant	500	1	H → Y May to be associated with polycystic ovary syndrome (PCOS); may be a common polymorphism. Ref.8		VAR_039809

Sequences

	Sequence		Length	Mass (Da)
	Q9BSK4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: FF0010DE5207D48F	FASTA	669	73,639
	10 20 30 40 50 60 MDLRTAVYNA ARDGKLQLLQ KLLSGRSREE LDELTGEVAG GGTPLLIAAR YGHLDVVEYL 70 80 90 100 110 120 VDRCGASVEA GGSVHFDGET IEGAPPLWAA SAAGHLDVVR SLLRRGASVN RTTRTNSTPL 130 140 150 160 170 180 RAACFDGHLE VVRYLVGEHQ ADLEVANRHG HTCLMISCYK GHREIARYLL EQGAQVNRRS 190 200 210 220 230 240 AKGNTALHDC AESGSLEILQ LLLGCKARME RDGYGMTPLL AASVTGHTNI VEYLIQEQPG 250 260 270 280 290 300 QEQVAGGEAQ PGLPQEDPST SQGCAQPQGA PCCSSSPEEP LNGESYESCC PTSREAAVEA 310 320 330 340 350 360 LELLGATYVD KKRDLLGALK HWRRAMELRH QGGEYLPKPE PPQLVLAYDY SREVNTTEEL 370 380 390 400 410 420 EALITDPDEM RMQALLIRER ILGPSHPDTS YYIRYRGAVY ADSGNFERCI RLWKYALDMQ 430 440 450 460 470 480 QSNLEPLSPM TASSFLSFAE LFSYVLQDRA AKGSLGTQIG FADLMGVLTK GVREVERALQ 490 500 510 520 530 540 LPREPGDSAQ FTKALAIILH LLYLLEKVEC TPSQEHLKHQ TVYRLLKCAP RGKNGFTPLH 550 560 570 580 590 600 MAVDKDTTNV GRYPVGRFPS LHVVKVLLDC GADPDSRDFD NNTPLHIAAQ NNCPAIMNAL 610 620 630 640 650 660 IEAGAHMDAT NAFKKTAYEL LDEKLLARGT MQPFNYVTLQ CLAARALDKN KIPYKGFIPE DLEAFIELH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel prostaglandin E receptor 4-associated protein participates in antiinflammatory signaling." Takayama K., Sukhova G.K., Chin M.T., Libby P. Circ. Res. 98:499-504(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH PTGER4.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[4]	"Full length sequencing of an human and murine muscular transcripts (Telethon Italy project B41)." Ievolella C., Zara I., Millino C., Faulkner G., Lanfranchi G. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-669. Tissue: Skeletal muscle.
[5]	The European IMAGE consortium Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 470-669.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 576-669. Tissue: Melanoma.
[7]	"The Fem1a gene is downregulated in Rhabdomyosarcoma." Ventura-Holman T., Hahn H., Subauste J.S., Maher J.F. Tumor Biol. 26:294-299(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[8]	"FEM1A is a candidate gene for polycystic ovary syndrome." Maher J.F., Hines R.S., Futterweit W., Crawford S., Lu D., Shen P., Oefner P., Kazi M., Wilson J.G., Subauste J.S., Cowan B.D. Gynecol. Endocrinol. 21:330-335(2005) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT TYR-500.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY356352 mRNA. Translation: AAR10439.1. AK315554 mRNA. Translation: BAG37930.1. BC004988 mRNA. Translation: AAH04988.1. AJ311359 mRNA. Translation: CAC85342.1. AL365408 mRNA. Translation: CAB96952.1. AL359589 mRNA. Translation: CAB94875.1. CR749732 mRNA. Translation: CAH18491.1.
IPI	IPI00013226.
PIR	T50619.
RefSeq	NP_061178.1. NM_018708.2.
UniGene	Hs.515082.
3D structure databases
HSSP	HSSP built from PDB template 1AWC based on UniProtKB Q00420.
ProteinModelPortal	Q9BSK4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BSK4. 1 interaction.
STRING	9606.ENSP00000269856.
PTM databases
PhosphoSite	Q9BSK4.
Polymorphism databases
DMDM	74752305.
Proteomic databases
PaxDb	Q9BSK4.
PRIDE	Q9BSK4.
Protocols and materials databases
DNASU	55527.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000269856; ENSP00000269856; ENSG00000141965.
GeneID	55527.
KEGG	hsa:55527.
UCSC	uc002mbf.3. human.
Organism-specific databases
CTD	55527.
GeneCards	GC19P004791.
H-InvDB	HIX0014672.
HGNC	HGNC:16934. FEM1A.
HPA	HPA016565.
MIM	613538. gene.
neXtProt	NX_Q9BSK4.
PharmGKB	PA134940973.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0666.
HOGENOM	HOG000008180.
HOVERGEN	HBG057774.
InParanoid	Q9BSK4.
OMA	GCKARME.
OrthoDB	EOG4RFKS3.
PhylomeDB	Q9BSK4.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Bgee	Q9BSK4.
CleanEx	HS_FEM1A.
Genevestigator	Q9BSK4.
Family and domain databases
Gene3D	1.25.40.10. 2 hits. 1.25.40.20. 4 hits.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR011990. TPR-like_helical. [Graphical view]
Pfam	PF00023. Ank. 1 hit. PF12796. Ank_2. 2 hits. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 9 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 2 hits.
PROSITE	PS50297. ANK_REP_REGION. 2 hits. PS50088. ANK_REPEAT. 7 hits. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	FEM1A. human.
GenomeRNAi	55527.
NextBio	59954.
SOURCE	Search...

Entry information

Entry name

FEM1A_HUMAN

Accession

Primary (citable) accession number: Q9BSK4
Secondary accession number(s): B2RDI3 , Q711P8, Q9NPN7, Q9NPW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.