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Protein

Solute carrier family 25 member 33

Gene

SLC25A33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial transporter that imports/exports pyrimidine nucleotides into and from mitochondria (PubMed:25320081). Transports preferentially uracil, thymine, and cytosine (deoxy)nucleoside di- and triphosphates by an antiport mechanism (PubMed:25320081). Also transports guanine but not adenine (deoxy)nucleotides (PubMed:25320081). Is inhibited strongly by pyridoxal 5'-phosphate, 4,7-diphenyl-1,10-phenanthroline, tannic acid, and mercurials (mercury dichloride, mersalyl acid, p-hydroxymercuribenzoate) (PubMed:25320081). Participates in mitochondrial genome maintenance, regulation of mitochondrial membrane potential and mitochondrial respiration (PubMed:20453889). Upon INS or IGF1 stimulation regulates cell growth and proliferation by controlling mitochondrial DNA replication and transcription, the ratio of mitochondria-to nuclear-encoded components of the electron transport chain resulting in control of mitochondrial ROS production (PubMed:20453889, PubMed:17596519). Participates in dendritic cell endocytosis and may associate with mitochondrial oxidative phosphorylation (PubMed:14715278).4 Publications

Kineticsi

The inhibitory constants (Ki) of these compounds are 174 um (UTP), 195 um (CTP), 318 um (GTP), and 333 um (ITP). UTP is the best substrate.1 Publication

  1. KM=0.16 mM for UTP (at 25 degrees Celsius)1 Publication
  2. KM=0.18 mM for CTP (at 25 degrees Celsius)1 Publication
  3. KM=0.08 mM for TTP (at 25 degrees Celsius)1 Publication
  4. KM=0.30 mM for GTP (at 25 degrees Celsius)1 Publication
  1. Vmax=98.5 µmol/min/g enzyme toward UTP (at 25 degrees Celsius)1 Publication
  2. Vmax=22.5 µmol/min/g enzyme toward CTP (at 25 degrees Celsius)1 Publication
  3. Vmax=50.8 µmol/min/g enzyme toward TTP (at 25 degrees Celsius)1 Publication
  4. Vmax=15.7 µmol/min/g enzyme toward GTP (at 25 degrees Celsius)1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to insulin-like growth factor stimulus Source: UniProtKB
  • cellular response to insulin stimulus Source: UniProtKB
  • mitochondrial DNA replication Source: UniProtKB
  • mitochondrial genome maintenance Source: UniProtKB
  • mitochondrial pyrimidine nucleotide import Source: UniProtKB
  • mitochondrial respiratory chain complex III assembly Source: UniProtKB
  • mitochondria-nucleus signaling pathway Source: UniProtKB
  • mitochondrion organization Source: UniProtKB
  • positive regulation of cell growth Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • pyrimidine nucleotide transport Source: UniProtKB
  • regulation of cell cycle arrest Source: UniProtKB
  • regulation of mitochondrial membrane potential Source: UniProtKB
  • regulation of oxidative phosphorylation Source: UniProtKB
  • regulation of reactive oxygen species biosynthetic process Source: UniProtKB
  • transcription from mitochondrial promoter Source: UniProtKB
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.29.10.7. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 25 member 33
Alternative name(s):
Bone marrow stromal cell mitochondrial carrier protein
Short name:
BMSC-MCP
Short name:
HuBMSC-MCP
Protein PNC1
Gene namesi
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:29681. SLC25A33.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei49 – 6517Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei121 – 14121Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei190 – 21021Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei233 – 25321Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei298 – 31821Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162403588.

Polymorphism and mutation databases

BioMutaiSLC25A33.
DMDMi74752304.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Solute carrier family 25 member 33PRO_0000291785Add
BLAST

Proteomic databases

EPDiQ9BSK2.
MaxQBiQ9BSK2.
PaxDbiQ9BSK2.
PeptideAtlasiQ9BSK2.
PRIDEiQ9BSK2.

PTM databases

iPTMnetiQ9BSK2.

Expressioni

Tissue specificityi

Expressed in the central nervous system. Also expressed in testis and skeletal muscle. Weakly expressed in heart, liver, kidney, prostate, colon and peripheral blood leukocytes.1 Publication

Inductioni

By INS or IGF1 through the PI-3 kinase/mTOR pathway.1 Publication

Gene expression databases

BgeeiQ9BSK2.
CleanExiHS_SLC25A33.
GenevisibleiQ9BSK2. HS.

Organism-specific databases

HPAiHPA049982.

Interactioni

Protein-protein interaction databases

BioGridi124002. 3 interactions.
IntActiQ9BSK2. 2 interactions.
MINTiMINT-6943347.
STRINGi9606.ENSP00000306328.

Structurei

3D structure databases

ProteinModelPortaliQ9BSK2.
SMRiQ9BSK2. Positions 13-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati9 – 118110Solcar 1Add
BLAST
Repeati126 – 21388Solcar 2Add
BLAST
Repeati231 – 31585Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
GeneTreeiENSGT00550000074670.
HOGENOMiHOG000188210.
HOVERGENiHBG059891.
InParanoidiQ9BSK2.
KOiK15116.
OMAiYLLEDHA.
OrthoDBiEOG7CCBRD.
PhylomeDBiQ9BSK2.
TreeFamiTF314220.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BSK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATGGQQKEN TLLHLFAGGC GGTVGAIFTC PLEVIKTRLQ SSRLALRTVY
60 70 80 90 100
YPQVHLGTIS GAGMVRPTSV TPGLFQVLKS ILEKEGPKSL FRGLGPNLVG
110 120 130 140 150
VAPSRAVYFA CYSKAKEQFN GIFVPNSNIV HIFSAGSAAF ITNSLMNPIW
160 170 180 190 200
MVKTRMQLEQ KVRGSKQMNT LQCARYVYQT EGIRGFYRGL TASYAGISET
210 220 230 240 250
IICFAIYESL KKYLKEAPLA SSANGTEKNS TSFFGLMAAA ALSKGCASCI
260 270 280 290 300
AYPHEVIRTR LREEGTKYKS FVQTARLVFR EEGYLAFYRG LFAQLIRQIP
310 320
NTAIVLSTYE LIVYLLEDRT Q
Length:321
Mass (Da):35,375
Last modified:June 1, 2001 - v1
Checksum:iA8B636704937A020
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti242 – 2421L → I.
Corresponds to variant rs35819756 [ dbSNP | Ensembl ].
VAR_032861

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF495714 mRNA. Translation: AAM18051.1.
AJ880283 mRNA. Translation: CAI54244.1.
AL954705, AL928921 Genomic DNA. Translation: CAI17271.1.
AL928921, AL954705 Genomic DNA. Translation: CAI17318.1.
BC004991 mRNA. Translation: AAH04991.1.
BC073135 mRNA. Translation: AAH73135.1.
CCDSiCCDS103.1.
RefSeqiNP_115691.1. NM_032315.2.
UniGeneiHs.568613.

Genome annotation databases

EnsembliENST00000302692; ENSP00000306328; ENSG00000171612.
GeneIDi84275.
KEGGihsa:84275.
UCSCiuc001apw.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF495714 mRNA. Translation: AAM18051.1.
AJ880283 mRNA. Translation: CAI54244.1.
AL954705, AL928921 Genomic DNA. Translation: CAI17271.1.
AL928921, AL954705 Genomic DNA. Translation: CAI17318.1.
BC004991 mRNA. Translation: AAH04991.1.
BC073135 mRNA. Translation: AAH73135.1.
CCDSiCCDS103.1.
RefSeqiNP_115691.1. NM_032315.2.
UniGeneiHs.568613.

3D structure databases

ProteinModelPortaliQ9BSK2.
SMRiQ9BSK2. Positions 13-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124002. 3 interactions.
IntActiQ9BSK2. 2 interactions.
MINTiMINT-6943347.
STRINGi9606.ENSP00000306328.

Protein family/group databases

TCDBi2.A.29.10.7. the mitochondrial carrier (mc) family.

PTM databases

iPTMnetiQ9BSK2.

Polymorphism and mutation databases

BioMutaiSLC25A33.
DMDMi74752304.

Proteomic databases

EPDiQ9BSK2.
MaxQBiQ9BSK2.
PaxDbiQ9BSK2.
PeptideAtlasiQ9BSK2.
PRIDEiQ9BSK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302692; ENSP00000306328; ENSG00000171612.
GeneIDi84275.
KEGGihsa:84275.
UCSCiuc001apw.4. human.

Organism-specific databases

CTDi84275.
GeneCardsiSLC25A33.
H-InvDBHIX0009627.
HGNCiHGNC:29681. SLC25A33.
HPAiHPA049982.
MIMi610816. gene.
neXtProtiNX_Q9BSK2.
PharmGKBiPA162403588.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
GeneTreeiENSGT00550000074670.
HOGENOMiHOG000188210.
HOVERGENiHBG059891.
InParanoidiQ9BSK2.
KOiK15116.
OMAiYLLEDHA.
OrthoDBiEOG7CCBRD.
PhylomeDBiQ9BSK2.
TreeFamiTF314220.

Miscellaneous databases

ChiTaRSiSLC25A33. human.
GenomeRNAii84275.
PROiQ9BSK2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BSK2.
CleanExiHS_SLC25A33.
GenevisibleiQ9BSK2. HS.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HuBMSC-MCP, a novel member of mitochondrial carrier superfamily, enhances dendritic cell endocytosis."
    Wang B., Li N., Sui L., Wu Y., Wang X., Wang Q., Xia D., Wan T., Cao X.
    Biochem. Biophys. Res. Commun. 314:292-300(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "The insulin-like growth factor-I-mTOR signaling pathway induces the mitochondrial pyrimidine nucleotide carrier to promote cell growth."
    Floyd S., Favre C., Lasorsa F.M., Leahy M., Trigiante G., Stroebel P., Marx A., Loughran G., O'Callaghan K., Marobbio C.M., Slotboom D.J., Kunji E.R., Palmieri F., O'Connor R.
    Mol. Biol. Cell 18:3545-3555(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION.
    Tissue: Testis.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye and Skin.
  5. "Fourteen novel human members of mitochondrial solute carrier family 25 (SLC25) widely expressed in the central nervous system."
    Haitina T., Lindblom J., Renstroem T., Fredriksson R.
    Genomics 88:779-790(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "Mitochondrial pyrimidine nucleotide carrier (PNC1) regulates mitochondrial biogenesis and the invasive phenotype of cancer cells."
    Favre C., Zhdanov A., Leahy M., Papkovsky D., O'Connor R.
    Oncogene 29:3964-3976(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "The human SLC25A33 and SLC25A36 genes of solute carrier family 25 encode two mitochondrial pyrimidine nucleotide transporters."
    Di Noia M.A., Todisco S., Cirigliano A., Rinaldi T., Agrimi G., Iacobazzi V., Palmieri F.
    J. Biol. Chem. 289:33137-33148(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiS2533_HUMAN
AccessioniPrimary (citable) accession number: Q9BSK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.