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Protein

Protein FAM64A

Gene

FAM64A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

During mitosis, may play a role in the control of metaphase-to-anaphase transition.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

SIGNORiQ9BSJ6.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein FAM64A
Alternative name(s):
CALM-interactor expressed in thymus and spleen
Regulator of chromosome segregation protein 1
Gene namesi
Name:FAM64A
Synonyms:CATS, RCS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:25483. FAM64A.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleolus Source: BHF-UCL
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi14 – 174RRSL → ARSA: Weak reduction in ubiquitination. Complete loss of ubiquitination; when associated with 53-A--A-56. 1 Publication
Mutagenesisi53 – 564RLPL → ALPA: Great reduction in ubiquitination. Complete loss of ubiquitination; when associated with 14-A--A-17. 1 Publication
Mutagenesisi129 – 1291S → A: No effect on phosphorylation. 1 Publication
Mutagenesisi131 – 1311S → A: Reduced phosphorylation. 1 Publication

Organism-specific databases

PharmGKBiPA142671874.

Polymorphism and mutation databases

BioMutaiFAM64A.
DMDMi74733018.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Protein FAM64APRO_0000281159Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineCombined sources
Modified residuei16 – 161PhosphoserineCombined sources
Modified residuei129 – 1291PhosphoserineCombined sources
Modified residuei131 – 1311Phosphoserine; by UHMK1; in vitroCombined sources1 Publication
Modified residuei199 – 1991PhosphoserineCombined sources
Modified residuei201 – 2011PhosphoserineCombined sources

Post-translational modificationi

Ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C) complex in the presence of FZR1, leading to its degradation by the proteasome during mitotic exit. However, degradation is not essential for normal mitotic progression within a single cell cycle.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9BSJ6.
MaxQBiQ9BSJ6.
PaxDbiQ9BSJ6.
PeptideAtlasiQ9BSJ6.
PRIDEiQ9BSJ6.

PTM databases

iPTMnetiQ9BSJ6.
PhosphoSiteiQ9BSJ6.

Expressioni

Tissue specificityi

Expressed in thymus (at protein level). Detected in spleen, colon, ovary and small intestines.2 Publications

Developmental stagei

Regulated in a cell-cycle dependent manner, with lowest levels in quiescent cells or at G1 phase. Progressive up-regulation starting at S phase and peaking at G2 and G2/M phases, followed by a drastic drop as cells exit mitosis (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000129195.
CleanExiHS_FAM64A.
ExpressionAtlasiQ9BSJ6. baseline and differential.
GenevisibleiQ9BSJ6. HS.

Organism-specific databases

HPAiHPA043783.
HPA049934.

Interactioni

Subunit structurei

Isoform 1 and isoform 2 interact with PICALM; this interaction may target PICALM to the nucleus. During mitosis, associates with HDAC2 and MTA2 subunits of the chromatin-remodeling NuRD complex; this association is strongest at prometaphase and decreases as the cell progresses through metaphase and anaphase.

Binary interactionsi

WithEntry#Exp.IntActNotes
AGTRAPQ6RW133EBI-2568609,EBI-741181
CCNDBP1O952733EBI-2568609,EBI-748961
CEP70Q8NHQ13EBI-2568609,EBI-739624
KRT40Q6A1623EBI-2568609,EBI-10171697
PRNPP041565EBI-2568609,EBI-977302
SSX2IPQ9Y2D83EBI-2568609,EBI-2212028
TFCP2Q128003EBI-2568609,EBI-717422
TRIM27P143733EBI-2568609,EBI-719493

Protein-protein interaction databases

BioGridi119983. 14 interactions.
DIPiDIP-56370N.
IntActiQ9BSJ6. 9 interactions.
MINTiMINT-8330036.
STRINGi9606.ENSP00000250056.

Structurei

3D structure databases

ProteinModelPortaliQ9BSJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi14 – 174D-box 1
Motifi53 – 564D-box 2

Domaini

The N-terminal destruction box 2 (D-box 2) is required for APC/C ubiquitination and proteasomal degradation.1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IXNZ. Eukaryota.
ENOG4111M05. LUCA.
GeneTreeiENSGT00390000008128.
HOGENOMiHOG000293378.
HOVERGENiHBG067744.
InParanoidiQ9BSJ6.
PhylomeDBiQ9BSJ6.
TreeFamiTF336322.

Family and domain databases

InterProiIPR009932. DUF1466.
[Graphical view]
PfamiPF07326. DUF1466. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BSJ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASRWQNMGT SVRRRSLQHQ EQLEDSKELQ PVVSHQETSV GALGSLCRQF
60 70 80 90 100
QRRLPLRAVN LNLRAGPSWK RLETPEPGQQ GLQAAARSAK SALGAVSQRI
110 120 130 140 150
QESCQSGTKW LVETQVKARR RKRGAQKGSG SPTHSLSQKS TRLSGAAPAH
160 170 180 190 200
SAADPWEKEH HRLSVRMGSH AHPLRRSRRE AAFRSPYSST EPLCSPSESD
210 220 230 240
SDLEPVGAGI QHLQKLSQEL DEAIMAEERK QALSDRQGFI LKDVYASP
Length:248
Mass (Da):27,480
Last modified:June 1, 2001 - v1
Checksum:iE981F1AF64375D5A
GO
Isoform 2 (identifier: Q9BSJ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     229-248: RKQALSDRQGFILKDVYASP → SGDIVSLIHD

Show »
Length:238
Mass (Da):26,242
Checksum:i161ECFDBE748D36B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581A → T in BAA91468 (PubMed:14702039).Curated
Sequence conflicti167 – 1671M → L in BAA91644 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti199 – 1991S → C.
Corresponds to variant rs16955870 [ dbSNP | Ensembl ].
VAR_056873

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei229 – 24820RKQAL…VYASP → SGDIVSLIHD in isoform 2. 2 PublicationsVSP_023997Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001018 mRNA. Translation: BAA91468.1.
AK001353 mRNA. Translation: BAA91644.1.
AC055872 Genomic DNA. No translation available.
BC005004 mRNA. Translation: AAH05004.1.
BC013966 mRNA. Translation: AAH13966.1.
CCDSiCCDS32541.1. [Q9BSJ6-2]
CCDS56016.1. [Q9BSJ6-1]
RefSeqiNP_001182157.1. NM_001195228.1. [Q9BSJ6-1]
NP_061886.2. NM_019013.2. [Q9BSJ6-2]
UniGeneiHs.592116.

Genome annotation databases

EnsembliENST00000250056; ENSP00000250056; ENSG00000129195. [Q9BSJ6-1]
ENST00000572447; ENSP00000459235; ENSG00000129195. [Q9BSJ6-2]
ENST00000576056; ENSP00000458534; ENSG00000129195. [Q9BSJ6-2]
GeneIDi54478.
KEGGihsa:54478.
UCSCiuc002gcu.3. human. [Q9BSJ6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001018 mRNA. Translation: BAA91468.1.
AK001353 mRNA. Translation: BAA91644.1.
AC055872 Genomic DNA. No translation available.
BC005004 mRNA. Translation: AAH05004.1.
BC013966 mRNA. Translation: AAH13966.1.
CCDSiCCDS32541.1. [Q9BSJ6-2]
CCDS56016.1. [Q9BSJ6-1]
RefSeqiNP_001182157.1. NM_001195228.1. [Q9BSJ6-1]
NP_061886.2. NM_019013.2. [Q9BSJ6-2]
UniGeneiHs.592116.

3D structure databases

ProteinModelPortaliQ9BSJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119983. 14 interactions.
DIPiDIP-56370N.
IntActiQ9BSJ6. 9 interactions.
MINTiMINT-8330036.
STRINGi9606.ENSP00000250056.

PTM databases

iPTMnetiQ9BSJ6.
PhosphoSiteiQ9BSJ6.

Polymorphism and mutation databases

BioMutaiFAM64A.
DMDMi74733018.

Proteomic databases

EPDiQ9BSJ6.
MaxQBiQ9BSJ6.
PaxDbiQ9BSJ6.
PeptideAtlasiQ9BSJ6.
PRIDEiQ9BSJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000250056; ENSP00000250056; ENSG00000129195. [Q9BSJ6-1]
ENST00000572447; ENSP00000459235; ENSG00000129195. [Q9BSJ6-2]
ENST00000576056; ENSP00000458534; ENSG00000129195. [Q9BSJ6-2]
GeneIDi54478.
KEGGihsa:54478.
UCSCiuc002gcu.3. human. [Q9BSJ6-1]

Organism-specific databases

CTDi54478.
GeneCardsiFAM64A.
HGNCiHGNC:25483. FAM64A.
HPAiHPA043783.
HPA049934.
neXtProtiNX_Q9BSJ6.
PharmGKBiPA142671874.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXNZ. Eukaryota.
ENOG4111M05. LUCA.
GeneTreeiENSGT00390000008128.
HOGENOMiHOG000293378.
HOVERGENiHBG067744.
InParanoidiQ9BSJ6.
PhylomeDBiQ9BSJ6.
TreeFamiTF336322.

Enzyme and pathway databases

SIGNORiQ9BSJ6.

Miscellaneous databases

GenomeRNAii54478.
PROiQ9BSJ6.

Gene expression databases

BgeeiENSG00000129195.
CleanExiHS_FAM64A.
ExpressionAtlasiQ9BSJ6. baseline and differential.
GenevisibleiQ9BSJ6. HS.

Family and domain databases

InterProiIPR009932. DUF1466.
[Graphical view]
PfamiPF07326. DUF1466. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFA64A_HUMAN
AccessioniPrimary (citable) accession number: Q9BSJ6
Secondary accession number(s): Q96CT4, Q9NVV1, Q9NWB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.