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Protein

RAD9, HUS1, RAD1-interacting nuclear orphan protein 1

Gene

RHNO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in DNA damage response (DDR) signaling upon genotoxic stresses such as ionizing radiation (IR) during the S phase. Recruited to sites of DNA damage through interaction with the 9-1-1 cell-cycle checkpoint response complex and TOPBP1 in a ATR-dependent manner. Required for the progression of the G1 to S phase transition. Plays a role in the stimulation of CHEK1 phosphorylation.1 Publication

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular response to ionizing radiation Source: UniProtKB
  • cellular response to UV Source: UniProtKB
  • DNA damage checkpoint Source: UniProtKB
  • positive regulation of G0 to G1 transition Source: UniProtKB
  • recombinational repair Source: UniProtKB

Keywordsi

Biological processCell cycle, DNA damage

Enzyme and pathway databases

ReactomeiR-HSA-5685938 HDR through Single Strand Annealing (SSA)
R-HSA-5693607 Processing of DNA double-strand break ends
R-HSA-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
RAD9, HUS1, RAD1-interacting nuclear orphan protein 1
Alternative name(s):
RAD9, RAD1, HUS1-interacting nuclear orphan protein
Gene namesi
Name:RHNO1
Synonyms:C12orf32, RHINO
ORF Names:HKMT1188
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000171792.10
HGNCiHGNC:28206 RHNO1
MIMi614085 gene
neXtProtiNX_Q9BSD3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55 – 61SWVSPDF → AAAAAAA: Inhibits binding to the 9-1-1 complex. Does not inhibit interaction with TOPBP1. Inhibits localizion to sites of DNA damage. 1 Publication7

Organism-specific databases

DisGeNETi83695
OpenTargetsiENSG00000171792

Polymorphism and mutation databases

BioMutaiRHNO1
DMDMi74739410

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002631051 – 238RAD9, HUS1, RAD1-interacting nuclear orphan protein 1Add BLAST238

Proteomic databases

MaxQBiQ9BSD3
PaxDbiQ9BSD3
PeptideAtlasiQ9BSD3
PRIDEiQ9BSD3

PTM databases

iPTMnetiQ9BSD3
PhosphoSitePlusiQ9BSD3

Expressioni

Tissue specificityi

Weakly expressed in testis, prostate, ovary, thymus and small intestine. Expressed strongly in breast cancer cells.1 Publication

Inductioni

Up-regulated in breast cancer cells.1 Publication

Gene expression databases

BgeeiENSG00000171792
CleanExiHS_C12orf32
ExpressionAtlasiQ9BSD3 baseline and differential
GenevisibleiQ9BSD3 HS

Interactioni

Subunit structurei

Interacts with RAD9A, RAD18, TOPBP1 and UBE2N.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi123729, 13 interactors
CORUMiQ9BSD3
IntActiQ9BSD3, 8 interactors
STRINGi9606.ENSP00000438590

Structurei

3D structure databases

ProteinModelPortaliQ9BSD3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG410IZA4 Eukaryota
ENOG410XXDT LUCA
GeneTreeiENSGT00390000003219
HOVERGENiHBG059694
InParanoidiQ9BSD3
OMAiTSWVSPQ
OrthoDBiEOG091G1492
PhylomeDBiQ9BSD3
TreeFamiTF336053

Family and domain databases

InterProiView protein in InterPro
IPR029293 RHNO1
PANTHERiPTHR35541 PTHR35541, 1 hit
PfamiView protein in Pfam
PF15319 RHINO, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BSD3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPRKKRRQP SQKAPLLFHQ QPLEGPKHSC ASTQLPITHT RQVPSKPIDH
60 70 80 90 100
STITSWVSPD FDTAAGSLFP AYQKHQNRAR HSSRKPTTSK FPHLTFESPQ
110 120 130 140 150
SSSSETLGIP LIRECPSESE KDVSRRPLVP VLSPQSCGNM SVQALQSLPY
160 170 180 190 200
VFIPPDIQTP ESSSVKEELI PQDQKENSLL SCTLHTGTPN SPEPGPVLVK
210 220 230
DTPEDKYGIK VTWRRRQHLL AYLRERGKLS RSQFLVKS
Length:238
Mass (Da):26,709
Last modified:June 1, 2001 - v1
Checksum:iC405083C6F6E4CAD
GO
Isoform 2 (identifier: Q9BSD3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-56: Missing.

Show »
Length:224
Mass (Da):25,160
Checksum:iA87E082158162395
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04530743 – 56Missing in isoform 2. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073599 mRNA Translation: BAD38639.1
AK304613 mRNA Translation: BAH14225.1
AC005911 Genomic DNA No translation available.
BC005106 mRNA No translation available.
CCDSiCCDS58199.1 [Q9BSD3-2]
CCDS8518.1 [Q9BSD3-1]
RefSeqiNP_001239428.1, NM_001252499.2 [Q9BSD3-1]
NP_001239429.1, NM_001252500.2 [Q9BSD3-2]
NP_001244026.1, NM_001257097.1 [Q9BSD3-1]
NP_001244027.1, NM_001257098.1 [Q9BSD3-1]
UniGeneiHs.198853

Genome annotation databases

EnsembliENST00000461997; ENSP00000438828; ENSG00000171792 [Q9BSD3-2]
ENST00000489288; ENSP00000438590; ENSG00000171792 [Q9BSD3-1]
ENST00000618250; ENSP00000479598; ENSG00000171792 [Q9BSD3-1]
GeneIDi83695
KEGGihsa:83695
UCSCiuc001qlh.5 human [Q9BSD3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRHNO1_HUMAN
AccessioniPrimary (citable) accession number: Q9BSD3
Secondary accession number(s): B7Z989
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health