Q9BSB4 (ATGA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy-related protein 101

Gene names

Name:	ATG101
Synonyms:	C12orf44
ORF Names:	PP894

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Autophagy factor required for autophagosome formation. Stabilizes ATG13, protecting it from proteasomal degradation. Ref.4 Ref.5
Subunit structure	Interacts with ATG13. Associates with a complex composed of ATG13, ULK1 and RB1CC1; the association with this complex requires the presence of ATG13. Ref.4 Ref.5
Subcellular location	Cytoplasm. Preautophagosomal structure. Note: Under starvation conditions, it is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. Ref.5
Sequence similarities	Belongs to the ATG101 family.
Sequence caution	The sequence AAG17273.1 differs from that shown. Reason: Frameshift at position 110.

Ontologies

Keywords
Biological process	Autophagy
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from mutant phenotype Ref.5. Source: UniProtKB
Cellular_component	pre-autophagosomal structure Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-2946739,EBI-2946739
ATG13	O75143	4	EBI-2946739,EBI-2798775
MAP1LC3C	Q9BXW4	2	EBI-2946739,EBI-2603996

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Autophagy-related protein 101		PRO_0000294322
Experimental info
	Sequence conflict	47	1	I → T in BAB13907. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BSB4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 69A881EAF9A07659	FASTA	218	25,003
	10 20 30 40 50 60 MNCRSEVLEV SVEGRQVEEA MLAVLHTVLL HRSTGKFHYK KEGTYSIGTV GTQDVDCDFI 70 80 90 100 110 120 DFTYVRVSSE ELDRALRKVV GEFKDALRNS GGDGLGQMSL EFYQKKKSRW PFSDECIPWE 130 140 150 160 170 180 VWTVKVHVVA LATEQERQIC REKVGEKLCE KIINIVEVMN RHEYLPKMPT QSEVDNVFDT 190 200 210 GLRDVQPYLY KISFQITDAL GTSVTTTMRR LIKDTLAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[2]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Uterus.
[4]	"A novel, human Atg13 binding protein, Atg101, interacts with ULK1 and is essential for macroautophagy." Mercer C.A., Kaliappan A., Dennis P.B. Autophagy 5:649-662(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG13, COMPLEX FORMATION WITH ULK1 AND RB1CC1.
[5]	"Atg101, a novel mammalian autophagy protein interacting with Atg13." Hosokawa N., Sasaki T., Iemura S.I., Natsume T., Hara T., Mizushima N. Autophagy 5:973-979(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS AUTOPHAGY FACTOR, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH ATG13.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK021835 mRNA. Translation: BAB13907.1. AF218031 mRNA. Translation: AAG17273.1. Frameshift. BC005151 mRNA. Translation: AAH05151.1. BC009937 mRNA. Translation: AAH09937.1.
IPI	IPI00305296.
RefSeq	NP_001092143.1. NM_001098673.1. NP_068753.2. NM_021934.4.
UniGene	Hs.9911.
3D structure databases
ProteinModelPortal	Q9BSB4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BSB4. 34 interactions.
STRING	9606.ENSP00000338990.
PTM databases
PhosphoSite	Q9BSB4.
Polymorphism databases
DMDM	74732983.
Proteomic databases
PaxDb	Q9BSB4.
PRIDE	Q9BSB4.
Protocols and materials databases
DNASU	60673.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336854; ENSP00000338990; ENSG00000123395.
GeneID	60673.
KEGG	hsa:60673.
UCSC	uc001rzu.4. human.
Organism-specific databases
CTD	60673.
GeneCards	GC12P052463.
H-InvDB	HIX0010645.
HGNC	HGNC:25679. C12orf44.
HPA	HPA039904.
MIM	615089. gene.
neXtProt	NX_Q9BSB4.
PharmGKB	PA143485374.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG315429.
HOGENOM	HOG000008086.
InParanoid	Q9BSB4.
OMA	QISLEFF.
OrthoDB	EOG4F7NKX.
PhylomeDB	Q9BSB4.
Gene expression databases
ArrayExpress	Q9BSB4.
Bgee	Q9BSB4.
CleanEx	HS_C12orf44.
Genevestigator	Q9BSB4.
Family and domain databases
InterPro	IPR012445. ATG101. [Graphical view]
PANTHER	PTHR13292. PTHR13292. 1 hit.
Pfam	PF07855. DUF1649. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	60673.
NextBio	65473.
SOURCE	Search...

Entry information

Entry name

ATGA1_HUMAN

Accession

Primary (citable) accession number: Q9BSB4
Secondary accession number(s): Q9HAE2, Q9HBN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.