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Q9BS31 (ZN649_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 649
Gene names
Name:ZNF649
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional repressor. Regulator of transcriptional factor complexes and may suppress SRE and AP-1 transcription activities mediated by growth factor signaling pathways. Ref.1

Subcellular location

Nucleus Ref.1.

Tissue specificity

Highly expressed in heart, skeletal muscle, and brain. Lower expression in liver, lung, kidney, pancreas and placenta. Ref.1

Developmental stage

Expressed in fetal heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Ref.1

Domain

The KRAB domain is required for transcriptional repression.

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 10 C2H2-type zinc fingers.

Contains 1 KRAB domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from direct assay PubMed 23580065. Source: UniProt

nucleus

Inferred from direct assay. Source: LIFEdb

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 23455924. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SUV39H1O434632EBI-4395789,EBI-349968

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 505505Zinc finger protein 649
PRO_0000251224

Regions

Domain8 – 7972KRAB
Zinc finger178 – 20023C2H2-type 1
Zinc finger206 – 22823C2H2-type 2
Zinc finger234 – 25623C2H2-type 3
Zinc finger262 – 28423C2H2-type 4
Zinc finger290 – 31223C2H2-type 5
Zinc finger318 – 34023C2H2-type 6
Zinc finger346 – 36823C2H2-type 7
Zinc finger374 – 39623C2H2-type 8
Zinc finger402 – 42423C2H2-type 9
Zinc finger430 – 45223C2H2-type 10

Natural variations

Natural variant3521G → D.
Corresponds to variant rs6509593 [ dbSNP | Ensembl ].
VAR_027668
Natural variant4691A → T.
Corresponds to variant rs1433083 [ dbSNP | Ensembl ].
VAR_027669

Experimental info

Sequence conflict81L → V in BAB14191. Ref.2
Sequence conflict3141G → E in BAB14191. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9BS31 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: EC1A70DCA8E01613

FASTA50557,683
        10         20         30         40         50         60 
MTKAQESLTL EDVAVDFTWE EWQFLSPAQK DLYRDVMLEN YSNLVSVGYQ AGKPDALTKL 

        70         80         90        100        110        120 
EQGEPLWTLE DEIHSPAHPE IEKADDHLQQ PLQNQKILKR TGQRYEHGRT LKSYLGLTNQ 

       130        140        150        160        170        180 
SRRYNRKEPA EFNGDGAFLH DNHEQMPTEI EFPESRKPIS TKSQFLKHQQ THNIEKAHEC 

       190        200        210        220        230        240 
TDCGKAFLKK SQLTEHKRIH TGKKPHVCSL CGKAFYKKYR LTEHERAHRG EKPHGCSLCG 

       250        260        270        280        290        300 
KAFYKRYRLT EHERAHKGEK PYGCSECGKA FPRKSELTEH QRIHTGIKPH QCSECGRAFS 

       310        320        330        340        350        360 
RKSLLVVHQR THTGEKPHTC SECGKGFIQK GNLNIHQRTH TGEKPYGCID CGKAFSQKSC 

       370        380        390        400        410        420 
LVAHQRYHTG KTPFVCPECG QPCSQKSGLI RHQKIHSGEK PYKCSDCGKA FLTKTMLIVH 

       430        440        450        460        470        480 
HRTHTGERPY GCDECEKAYF YMSCLVKHKR IHSREKRGDS VKVENPSTAS HSLSPSEHVQ 

       490        500 
GKSPVNMVTV AMVAGQCEFA HILHS 

« Hide

References

« Hide 'large scale' references
[1]"ZNF649, a novel Kruppel type zinc-finger protein, functions as a transcriptional suppressor."
Yang H., Yuan W., Wang Y., Zhu C., Liu B., Wang Y., Yang D., Li Y., Wang C., Wu X., Liu M.
Biochem. Biophys. Res. Commun. 333:206-215(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Teratocarcinoma.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[4]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK022706 mRNA. Translation: BAB14191.1.
AK315487 mRNA. Translation: BAG37871.1.
AL713677 mRNA. Translation: CAD28482.1.
AC011460 Genomic DNA. No translation available.
CH471135 Genomic DNA. Translation: EAW72050.1.
BC005368 mRNA. Translation: AAH05368.1.
CCDSCCDS12843.1.
RefSeqNP_075562.2. NM_023074.3.
UniGeneHs.148322.

3D structure databases

ProteinModelPortalQ9BS31.
SMRQ9BS31. Positions 2-49, 160-484.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122414. 3 interactions.
IntActQ9BS31. 2 interactions.
MINTMINT-4719207.

PTM databases

PhosphoSiteQ9BS31.

Polymorphism databases

DMDM74761227.

Proteomic databases

MaxQBQ9BS31.
PaxDbQ9BS31.
PRIDEQ9BS31.

Protocols and materials databases

DNASU65251.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000354957; ENSP00000347043; ENSG00000198093.
GeneID65251.
KEGGhsa:65251.
UCSCuc002pxy.3. human.

Organism-specific databases

CTD65251.
GeneCardsGC19M052392.
HGNCHGNC:25741. ZNF649.
HPAHPA026541.
MIM611903. gene.
neXtProtNX_Q9BS31.
PharmGKBPA143485678.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000234617.
HOVERGENHBG018163.
InParanoidQ9BS31.
KOK09228.
OMAKTMLIVH.
OrthoDBEOG7KSX7Q.
PhylomeDBQ9BS31.
TreeFamTF340593.

Enzyme and pathway databases

ReactomeREACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ9BS31.
BgeeQ9BS31.
CleanExHS_ZNF649.
GenevestigatorQ9BS31.

Family and domain databases

Gene3D3.30.160.60. 10 hits.
InterProIPR001909. Krueppel-associated_box.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF01352. KRAB. 1 hit.
[Graphical view]
SMARTSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 10 hits.
[Graphical view]
SUPFAMSSF109640. SSF109640. 1 hit.
PROSITEPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 10 hits.
PS50157. ZINC_FINGER_C2H2_2. 10 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZNF649. human.
GeneWikiZNF649.
GenomeRNAi65251.
NextBio67374.
PROQ9BS31.
SOURCESearch...

Entry information

Entry nameZN649_HUMAN
AccessionPrimary (citable) accession number: Q9BS31
Secondary accession number(s): A8MYJ5, B2RDC4, Q9H9N2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM