Q9BS31 (ZN649_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 649

Gene names

Name:

ZNF649

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional repressor. Regulator of transcriptional factor complexes and may suppress SRE and AP-1 transcription activities mediated by growth factor signaling pathways. Ref.1
Subcellular location	Nucleus Ref.1.
Tissue specificity	Highly expressed in heart, skeletal muscle, and brain. Lower expression in liver, lung, kidney, pancreas and placenta. Ref.1
Developmental stage	Expressed in fetal heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Ref.1
Domain	The KRAB domain is required for transcriptional repression.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 10 C2H2-type zinc fingers. Contains 1 KRAB domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay. Source: LIFEdb
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Zinc finger protein 649		PRO_0000251224
Regions
	Domain	8 – 79	72	KRAB
	Zinc finger	178 – 200	23	C2H2-type 1
	Zinc finger	206 – 228	23	C2H2-type 2
	Zinc finger	234 – 256	23	C2H2-type 3
	Zinc finger	262 – 284	23	C2H2-type 4
	Zinc finger	290 – 312	23	C2H2-type 5
	Zinc finger	318 – 340	23	C2H2-type 6
	Zinc finger	346 – 368	23	C2H2-type 7
	Zinc finger	374 – 396	23	C2H2-type 8
	Zinc finger	402 – 424	23	C2H2-type 9
	Zinc finger	430 – 452	23	C2H2-type 10
Natural variations
	Natural variant	352	1	G → D. Corresponds to variant rs6509593 [ dbSNP | Ensembl ].		VAR_027668
	Natural variant	469	1	A → T. Corresponds to variant rs1433083 [ dbSNP | Ensembl ].		VAR_027669
Experimental info
	Sequence conflict	8	1	L → V in BAB14191. Ref.2
	Sequence conflict	314	1	G → E in BAB14191. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9BS31 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EC1A70DCA8E01613	FASTA	505	57,683
	10 20 30 40 50 60 MTKAQESLTL EDVAVDFTWE EWQFLSPAQK DLYRDVMLEN YSNLVSVGYQ AGKPDALTKL 70 80 90 100 110 120 EQGEPLWTLE DEIHSPAHPE IEKADDHLQQ PLQNQKILKR TGQRYEHGRT LKSYLGLTNQ 130 140 150 160 170 180 SRRYNRKEPA EFNGDGAFLH DNHEQMPTEI EFPESRKPIS TKSQFLKHQQ THNIEKAHEC 190 200 210 220 230 240 TDCGKAFLKK SQLTEHKRIH TGKKPHVCSL CGKAFYKKYR LTEHERAHRG EKPHGCSLCG 250 260 270 280 290 300 KAFYKRYRLT EHERAHKGEK PYGCSECGKA FPRKSELTEH QRIHTGIKPH QCSECGRAFS 310 320 330 340 350 360 RKSLLVVHQR THTGEKPHTC SECGKGFIQK GNLNIHQRTH TGEKPYGCID CGKAFSQKSC 370 380 390 400 410 420 LVAHQRYHTG KTPFVCPECG QPCSQKSGLI RHQKIHSGEK PYKCSDCGKA FLTKTMLIVH 430 440 450 460 470 480 HRTHTGERPY GCDECEKAYF YMSCLVKHKR IHSREKRGDS VKVENPSTAS HSLSPSEHVQ 490 500 GKSPVNMVTV AMVAGQCEFA HILHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ZNF649, a novel Kruppel type zinc-finger protein, functions as a transcriptional suppressor." Yang H., Yuan W., Wang Y., Zhu C., Liu B., Wang Y., Yang D., Li Y., Wang C., Wu X., Liu M. Biochem. Biophys. Res. Commun. 333:206-215(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Teratocarcinoma.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK022706 mRNA. Translation: BAB14191.1. AK315487 mRNA. Translation: BAG37871.1. AL713677 mRNA. Translation: CAD28482.1. AC011460 Genomic DNA. No translation available. CH471135 Genomic DNA. Translation: EAW72050.1. BC005368 mRNA. Translation: AAH05368.1.
IPI	IPI00011987.
RefSeq	NP_075562.2. NM_023074.3.
UniGene	Hs.148322.
3D structure databases
ProteinModelPortal	Q9BS31.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BS31. 1 interaction.
MINT	MINT-4719207.
PTM databases
PhosphoSite	Q9BS31.
Polymorphism databases
DMDM	74761227.
Proteomic databases
PaxDb	Q9BS31.
PRIDE	Q9BS31.
Protocols and materials databases
DNASU	65251.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000354957; ENSP00000347043; ENSG00000198093.
GeneID	65251.
KEGG	hsa:65251.
UCSC	uc002pxy.3. human.
Organism-specific databases
CTD	65251.
GeneCards	GC19M052392.
HGNC	HGNC:25741. ZNF649.
HPA	HPA026541.
MIM	611903. gene.
neXtProt	NX_Q9BS31.
PharmGKB	PA143485678.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000234617.
HOVERGEN	HBG018163.
InParanoid	Q9BS31.
KO	K09228.
OMA	KTMLIVH.
OrthoDB	EOG4DR9C0.
PhylomeDB	Q9BS31.
Enzyme and pathway databases
Reactome	REACT_71. Gene Expression.
Gene expression databases
Bgee	Q9BS31.
CleanEx	HS_ZNF649.
Genevestigator	Q9BS31.
GermOnline	ENSG00000198093. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 10 hits.
InterPro	IPR001909. Krueppel-associated_box. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF01352. KRAB. 1 hit. [Graphical view]
SMART	SM00349. KRAB. 1 hit. SM00355. ZnF_C2H2. 10 hits. [Graphical view]
SUPFAM	SSF109640. Krueppel-associated_box. 1 hit.
PROSITE	PS50805. KRAB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 10 hits. PS50157. ZINC_FINGER_C2H2_2. 10 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZNF649. human.
GenomeRNAi	65251.
NextBio	67374.
SOURCE	Search...

Entry information

Entry name

ZN649_HUMAN

Accession

Primary (citable) accession number: Q9BS31
Secondary accession number(s): A8MYJ5, B2RDC4, Q9H9N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	June 1, 2001
Last modified:	May 29, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.