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Protein

Endoplasmic reticulum resident protein 44

Gene

ERP44

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1A and ERO1B in the endoplasmic reticulum.2 Publications

GO - Molecular functioni

  • protein disulfide isomerase activity Source: UniProtKB

GO - Biological processi

  • cell redox homeostasis Source: UniProtKB
  • glycoprotein metabolic process Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • protein folding Source: UniProtKB
  • response to endoplasmic reticulum stress Source: UniProtKB
  • response to unfolded protein Source: UniProtKB

Keywordsi

Molecular functionChaperone
Biological processStress response

Enzyme and pathway databases

ReactomeiR-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum resident protein 44
Short name:
ER protein 44
Short name:
ERp44
Alternative name(s):
Thioredoxin domain-containing protein 4
Gene namesi
Name:ERP44
Synonyms:KIAA0573, TXNDC4
ORF Names:UNQ532/PRO1075
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000023318.7.
HGNCiHGNC:18311. ERP44.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

DisGeNETi23071.
OpenTargetsiENSG00000023318.
PharmGKBiPA164719295.

Polymorphism and mutation databases

BioMutaiERP44.
DMDMi31077035.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Add BLAST29
ChainiPRO_000003418030 – 406Endoplasmic reticulum resident protein 44Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi58Interchain (with ERO1A)1 Publication
Disulfide bondi189 ↔ 2411 Publication
Disulfide bondi301 ↔ 3181 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ9BS26.
MaxQBiQ9BS26.
PaxDbiQ9BS26.
PeptideAtlasiQ9BS26.
PRIDEiQ9BS26.

2D gel databases

REPRODUCTION-2DPAGEiIPI00401264.

PTM databases

iPTMnetiQ9BS26.
PhosphoSitePlusiQ9BS26.
SwissPalmiQ9BS26.

Expressioni

Inductioni

Up-regulated by inducers of the unfolded protein response (UPR), including tunicamycin and dithiothreitol.1 Publication

Gene expression databases

BgeeiENSG00000023318.
CleanExiHS_TXNDC4.
GenevisibleiQ9BS26. HS.

Organism-specific databases

HPAiHPA001318.

Interactioni

Subunit structurei

Forms mixed disulfides with both ERO1A and ERO1B and cargo folding intermediates. Directly interacts with ITPR1 in a pH-, redox state- and calcium-dependent manner, but not with ITPR2 or ITPR3. The strength of this interaction inversely correlates with calcium concentration (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
LMAN1P492573EBI-541644,EBI-1057738

Protein-protein interaction databases

BioGridi116704. 81 interactors.
IntActiQ9BS26. 30 interactors.
MINTiMINT-2816102.
STRINGi9606.ENSP00000262455.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 33Combined sources4
Turni36 – 38Combined sources3
Helixi39 – 45Combined sources7
Beta strandi47 – 54Combined sources8
Helixi59 – 78Combined sources20
Turni81 – 83Combined sources3
Beta strandi86 – 91Combined sources6
Turni92 – 94Combined sources3
Helixi96 – 101Combined sources6
Beta strandi106 – 114Combined sources9
Beta strandi117 – 122Combined sources6
Helixi129 – 140Combined sources12
Beta strandi145 – 147Combined sources3
Helixi151 – 154Combined sources4
Beta strandi158 – 160Combined sources3
Beta strandi162 – 166Combined sources5
Beta strandi170 – 172Combined sources3
Helixi173 – 185Combined sources13
Turni186 – 188Combined sources3
Beta strandi189 – 194Combined sources6
Helixi196 – 199Combined sources4
Helixi200 – 202Combined sources3
Beta strandi206 – 212Combined sources7
Beta strandi214 – 218Combined sources5
Helixi230 – 241Combined sources12
Beta strandi244 – 247Combined sources4
Turni250 – 252Combined sources3
Helixi253 – 258Combined sources6
Beta strandi263 – 268Combined sources6
Helixi273 – 286Combined sources14
Helixi288 – 290Combined sources3
Turni291 – 293Combined sources3
Beta strandi294 – 300Combined sources7
Turni301 – 303Combined sources3
Helixi305 – 310Combined sources6
Helixi315 – 317Combined sources3
Beta strandi319 – 324Combined sources6
Beta strandi326 – 331Combined sources6
Helixi335 – 339Combined sources5
Helixi343 – 352Combined sources10
Helixi355 – 362Combined sources8
Helixi387 – 390Combined sources4
Turni394 – 396Combined sources3
Beta strandi397 – 400Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2R2JX-ray2.60A30-406[»]
5GU6X-ray2.00A30-402[»]
5GU7X-ray2.05C30-402[»]
5HQPX-ray2.60C/D30-406[»]
ProteinModelPortaliQ9BS26.
SMRiQ9BS26.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BS26.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 138ThioredoxinPROSITE-ProRule annotationAdd BLAST109

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 285Interaction with ITPR1By similarityAdd BLAST50

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi403 – 406Prevents secretion from ERPROSITE-ProRule annotation4

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0912. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000007707.
InParanoidiQ9BS26.
KOiK17264.
OMAiPEYDIFR.
OrthoDBiEOG091G08L8.
PhylomeDBiQ9BS26.
TreeFamiTF106378.

Family and domain databases

InterProiView protein in InterPro
IPR036249. Thioredoxin-like_sf.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF00085. Thioredoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 3 hits.
PROSITEiView protein in PROSITE
PS00014. ER_TARGET. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BS26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPAVFLSLP DLRCSLLLLV TWVFTPVTTE ITSLDTENID EILNNADVAL
60 70 80 90 100
VNFYADWCRF SQMLHPIFEE ASDVIKEEFP NENQVVFARV DCDQHSDIAQ
110 120 130 140 150
RYRISKYPTL KLFRNGMMMK REYRGQRSVK ALADYIRQQK SDPIQEIRDL
160 170 180 190 200
AEITTLDRSK RNIIGYFEQK DSDNYRVFER VANILHDDCA FLSAFGDVSK
210 220 230 240 250
PERYSGDNII YKPPGHSAPD MVYLGAMTNF DVTYNWIQDK CVPLVREITF
260 270 280 290 300
ENGEELTEEG LPFLILFHMK EDTESLEIFQ NEVARQLISE KGTINFLHAD
310 320 330 340 350
CDKFRHPLLH IQKTPADCPV IAIDSFRHMY VFGDFKDVLI PGKLKQFVFD
360 370 380 390 400
LHSGKLHREF HHGPDPTDTA PGEQAQDVAS SPPESSFQKL APSEYRYTLL

RDRDEL
Length:406
Mass (Da):46,971
Last modified:June 1, 2001 - v1
Checksum:i3865DCF3811BC263
GO

Sequence cautioni

The sequence BAA25499 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35D → A in AAQ89407 (PubMed:12975309).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ344330 mRNA. Translation: CAC87611.1.
AB011145 mRNA. Translation: BAA25499.1. Different initiation.
AY359048 mRNA. Translation: AAQ89407.1.
AK075024 mRNA. Translation: BAG52054.1.
AL360084 Genomic DNA. No translation available.
AL137072 Genomic DNA. No translation available.
AL358937 Genomic DNA. No translation available.
BC005374 mRNA. Translation: AAH05374.1.
CCDSiCCDS35082.1.
RefSeqiNP_055866.1. NM_015051.2.
UniGeneiHs.154023.

Genome annotation databases

EnsembliENST00000262455; ENSP00000262455; ENSG00000023318.
GeneIDi23071.
KEGGihsa:23071.
UCSCiuc004bam.4. human.

Similar proteinsi

Entry informationi

Entry nameiERP44_HUMAN
AccessioniPrimary (citable) accession number: Q9BS26
Secondary accession number(s): O60319
, Q4VXC1, Q5VWZ7, Q6UW14, Q8WX67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: June 1, 2001
Last modified: November 22, 2017
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references