Q9BS16 (CENPK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere protein K
Short name=CENP-K

Alternative name(s):
Interphase centromere complex protein 37
Protein AF-5alpha
p33

Gene names

Name:	CENPK
Synonyms:	ICEN37
ORF Names:	FKSG14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. Ref.5 Ref.6 Ref.8
Subunit structure	Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with CENPH. Ref.6 Ref.7 Ref.9
Subcellular location	Nucleus. Chromosome › centromere. Chromosome › centromere › kinetochore. Note: Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex. Ref.5 Ref.6
Tissue specificity	Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta. Ref.1
Involvement in disease	Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with MLL.
Sequence similarities	Belongs to the CENPK family.

Ontologies

Keywords
Cellular component	Centromere Chromosome Kinetochore Nucleus
Coding sequence diversity	Chromosomal rearrangement
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CENP-A containing nucleosome assembly at centromere Traceable author statement. Source: Reactome mitotic prometaphase Traceable author statement. Source: Reactome positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome nucleoplasm Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Centromere protein K		PRO_0000249482
Regions
	Coiled coil	22 – 42	21	Potential
	Coiled coil	98 – 151	54	Potential
Sites
	Site	96 – 97	2	Breakpoint for translocation to form MLL-CENPK oncogene
Experimental info
	Sequence conflict	45	1	I → M in AAG31004. Ref.3
	Sequence conflict	59	1	L → W in AAG31004. Ref.3
	Sequence conflict	66	1	C → G in AAG31004. Ref.3
	Sequence conflict	74	1	W → G in AAG31004. Ref.3
	Sequence conflict	81	1	T → I in AAG31004. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9BS16 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D59737D32D221A84	FASTA	269	31,655
	10 20 30 40 50 60 MNQEDLDPDS TTDVGDVTNT EEELIRECEE MWKDMEECQN KLSLIGTETL TDSNAQLSLL 70 80 90 100 110 120 IMQVKCLTAE LSQWQKKTPE TIPLTEDVLI TLGKEEFQKL RQDLEMVLST KESKNEKLKE 130 140 150 160 170 180 DLEREQRWLD EQQQIMESLN VLHSELKNKV ETFSESRIFN ELKTKMLNIK EYKEKLLSTL 190 200 210 220 230 240 GEFLEDHFPL PDRSVKKKKK NIQESSVNLI TLHEMLEILI NRLFDVPHDP YVKISDSFWP 250 260 PYVELLLRNG IALRHPEDPT RIRLEAFHQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fusion of the MLL gene with two different genes, AF-6 and AF-5alpha, by a complex translocation involving chromosomes 5, 6, 8 and 11 in infant leukemia." Taki T., Hayashi Y., Taniwaki M., Seto M., Ueda R., Hanada R., Suzukawa K., Yokota J., Morishita K. Oncogene 13:2121-2130(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHROMOSOMAL TRANSLOCATION WITH MLL, TISSUE SPECIFICITY.
[2]	"Molecular cloning of human p33." Chang M.S., Yang Y.C. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Molecular cloning of FKSG14, a novel gene located on chromosome 5." Wang Y.-G. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Carcinoma.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Gall bladder.
[5]	"Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells." Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K. Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres." Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T. Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH CENPH; CENPI; CENPN; CENPO; CENPP; CENPQ; CENPR AND CENPU, FUNCTION, SUBCELLULAR LOCATION.
[7]	"The human CENP-A centromeric nucleosome-associated complex." Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III, Cleveland D.W. Nat. Cell Biol. 8:458-469(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE CENPA-CAD COMPLEX WITH CENPI; CENPL; CENPO; CENPP; CENPQ; CENPR AND CENPS.
[8]	"KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates." Cheeseman I.M., Hori T., Fukagawa T., Desai A. Mol. Biol. Cell 19:587-594(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"CENP-K and CENP-H may form coiled-coils in the kinetochores." Qiu S., Wang J., Yu C., He D. Sci. China, Ser. C, Life Sci. 52:352-359(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CENPH.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY033646 mRNA. Translation: AAK59382.1. AF315941 mRNA. Translation: AAL26880.1. AY009151 mRNA. Translation: AAG31004.1. BC005400 mRNA. Translation: AAH05400.1. BC008504 mRNA. Translation: AAH08504.1.
IPI	IPI00074020.
RefSeq	NP_001253967.1. NM_001267038.1. NP_071428.2. NM_022145.4.
UniGene	Hs.529778.
3D structure databases
ProteinModelPortal	Q9BS16.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000242872.
PTM databases
PhosphoSite	Q9BS16.
Polymorphism databases
DMDM	74732954.
Proteomic databases
PaxDb	Q9BS16.
PRIDE	Q9BS16.
Protocols and materials databases
DNASU	64105.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000242872; ENSP00000242872; ENSG00000123219. ENST00000396679; ENSP00000379911; ENSG00000123219. ENST00000514814; ENSP00000422421; ENSG00000123219.
GeneID	64105.
KEGG	hsa:64105.
UCSC	uc003jts.3. human.
Organism-specific databases
CTD	64105.
GeneCards	GC05M064850.
HGNC	HGNC:29479. CENPK.
MIM	611502. gene.
neXtProt	NX_Q9BS16.
PharmGKB	PA145149171.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46525.
HOGENOM	HOG000111542.
HOVERGEN	HBG081084.
InParanoid	Q9BS16.
KO	K11503.
OMA	SQWQKET.
OrthoDB	EOG4X6C8X.
PhylomeDB	Q9BS16.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases.
Gene expression databases
ArrayExpress	Q9BS16.
Bgee	Q9BS16.
CleanEx	HS_CENPK.
Genevestigator	Q9BS16.
GermOnline	ENSG00000123219. Homo sapiens.
Family and domain databases
InterPro	IPR020993. Centromere_CenpK. [Graphical view]
Pfam	PF11802. CENP-K. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	64105.
NextBio	65948.
SOURCE	Search...

Entry information

Entry name

CENPK_HUMAN

Accession

Primary (citable) accession number: Q9BS16
Secondary accession number(s): Q9H4L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.