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Protein

Centromere protein K

Gene

CENPK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei96 – 972Breakpoint for translocation to form KMT2A/MLL1-CENPK oncogene

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_355252. RHO GTPases Activate Formins.
REACT_682. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein K
Short name:
CENP-K
Alternative name(s):
Interphase centromere complex protein 37
Protein AF-5alpha
p33
Gene namesi
Name:CENPK
Synonyms:ICEN37
ORF Names:FKSG14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:29479. CENPK.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Involvement in diseasei

Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.

Organism-specific databases

PharmGKBiPA145149171.

Polymorphism and mutation databases

BioMutaiCENPK.
DMDMi74732954.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Centromere protein KPRO_0000249482Add
BLAST

Proteomic databases

MaxQBiQ9BS16.
PaxDbiQ9BS16.
PRIDEiQ9BS16.

PTM databases

PhosphoSiteiQ9BS16.

Expressioni

Tissue specificityi

Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.1 Publication

Gene expression databases

BgeeiQ9BS16.
CleanExiHS_CENPK.
ExpressionAtlasiQ9BS16. baseline and differential.
GenevisibleiQ9BS16. HS.

Interactioni

Subunit structurei

Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts directly with CENPH.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Q86V283EBI-6871750,EBI-10259496
CENPHQ9H3R53EBI-6871750,EBI-1003700
MRFAP1L1Q96HT83EBI-6871750,EBI-748896

Protein-protein interaction databases

BioGridi122063. 10 interactions.
IntActiQ9BS16. 4 interactions.
STRINGi9606.ENSP00000242872.

Structurei

3D structure databases

ProteinModelPortaliQ9BS16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili22 – 4221Sequence AnalysisAdd
BLAST
Coiled coili98 – 15154Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the CENPK family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG46525.
GeneTreeiENSGT00390000006243.
HOGENOMiHOG000111542.
HOVERGENiHBG081084.
InParanoidiQ9BS16.
KOiK11503.
OMAiSQWQKET.
OrthoDBiEOG780RN0.
PhylomeDBiQ9BS16.
TreeFamiTF333264.

Family and domain databases

InterProiIPR020993. Centromere_CenpK.
[Graphical view]
PfamiPF11802. CENP-K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BS16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQEDLDPDS TTDVGDVTNT EEELIRECEE MWKDMEECQN KLSLIGTETL
60 70 80 90 100
TDSNAQLSLL IMQVKCLTAE LSQWQKKTPE TIPLTEDVLI TLGKEEFQKL
110 120 130 140 150
RQDLEMVLST KESKNEKLKE DLEREQRWLD EQQQIMESLN VLHSELKNKV
160 170 180 190 200
ETFSESRIFN ELKTKMLNIK EYKEKLLSTL GEFLEDHFPL PDRSVKKKKK
210 220 230 240 250
NIQESSVNLI TLHEMLEILI NRLFDVPHDP YVKISDSFWP PYVELLLRNG
260
IALRHPEDPT RIRLEAFHQ
Length:269
Mass (Da):31,655
Last modified:June 1, 2001 - v1
Checksum:iD59737D32D221A84
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451I → M in AAG31004 (Ref. 3) Curated
Sequence conflicti59 – 591L → W in AAG31004 (Ref. 3) Curated
Sequence conflicti66 – 661C → G in AAG31004 (Ref. 3) Curated
Sequence conflicti74 – 741W → G in AAG31004 (Ref. 3) Curated
Sequence conflicti81 – 811T → I in AAG31004 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY033646 mRNA. Translation: AAK59382.1.
AF315941 mRNA. Translation: AAL26880.1.
AY009151 mRNA. Translation: AAG31004.1.
BC005400 mRNA. Translation: AAH05400.1.
BC008504 mRNA. Translation: AAH08504.1.
CCDSiCCDS3984.1.
RefSeqiNP_001253967.1. NM_001267038.1.
NP_071428.2. NM_022145.4.
UniGeneiHs.529778.

Genome annotation databases

EnsembliENST00000242872; ENSP00000242872; ENSG00000123219.
ENST00000396679; ENSP00000379911; ENSG00000123219.
ENST00000514814; ENSP00000422421; ENSG00000123219.
GeneIDi64105.
KEGGihsa:64105.
UCSCiuc003jts.3. human.

Keywords - Coding sequence diversityi

Chromosomal rearrangement

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY033646 mRNA. Translation: AAK59382.1.
AF315941 mRNA. Translation: AAL26880.1.
AY009151 mRNA. Translation: AAG31004.1.
BC005400 mRNA. Translation: AAH05400.1.
BC008504 mRNA. Translation: AAH08504.1.
CCDSiCCDS3984.1.
RefSeqiNP_001253967.1. NM_001267038.1.
NP_071428.2. NM_022145.4.
UniGeneiHs.529778.

3D structure databases

ProteinModelPortaliQ9BS16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122063. 10 interactions.
IntActiQ9BS16. 4 interactions.
STRINGi9606.ENSP00000242872.

PTM databases

PhosphoSiteiQ9BS16.

Polymorphism and mutation databases

BioMutaiCENPK.
DMDMi74732954.

Proteomic databases

MaxQBiQ9BS16.
PaxDbiQ9BS16.
PRIDEiQ9BS16.

Protocols and materials databases

DNASUi64105.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000242872; ENSP00000242872; ENSG00000123219.
ENST00000396679; ENSP00000379911; ENSG00000123219.
ENST00000514814; ENSP00000422421; ENSG00000123219.
GeneIDi64105.
KEGGihsa:64105.
UCSCiuc003jts.3. human.

Organism-specific databases

CTDi64105.
GeneCardsiGC05M064850.
HGNCiHGNC:29479. CENPK.
MIMi611502. gene.
neXtProtiNX_Q9BS16.
PharmGKBiPA145149171.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG46525.
GeneTreeiENSGT00390000006243.
HOGENOMiHOG000111542.
HOVERGENiHBG081084.
InParanoidiQ9BS16.
KOiK11503.
OMAiSQWQKET.
OrthoDBiEOG780RN0.
PhylomeDBiQ9BS16.
TreeFamiTF333264.

Enzyme and pathway databases

ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_355252. RHO GTPases Activate Formins.
REACT_682. Mitotic Prometaphase.

Miscellaneous databases

GeneWikiiCENPK.
GenomeRNAii64105.
NextBioi65948.
PROiQ9BS16.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BS16.
CleanExiHS_CENPK.
ExpressionAtlasiQ9BS16. baseline and differential.
GenevisibleiQ9BS16. HS.

Family and domain databases

InterProiIPR020993. Centromere_CenpK.
[Graphical view]
PfamiPF11802. CENP-K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Fusion of the MLL gene with two different genes, AF-6 and AF-5alpha, by a complex translocation involving chromosomes 5, 6, 8 and 11 in infant leukemia."
    Taki T., Hayashi Y., Taniwaki M., Seto M., Ueda R., Hanada R., Suzukawa K., Yokota J., Morishita K.
    Oncogene 13:2121-2130(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHROMOSOMAL TRANSLOCATION WITH KMT2A/MLL1, TISSUE SPECIFICITY.
  2. "Molecular cloning of human p33."
    Chang M.S., Yang Y.C.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Molecular cloning of FKSG14, a novel gene located on chromosome 5."
    Wang Y.-G.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Carcinoma.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Gall bladder.
  5. "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells."
    Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K.
    Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres."
    Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T.
    Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH CENPH; CENPI; CENPN; CENPO; CENPP; CENPQ; CENPR AND CENPU, FUNCTION, SUBCELLULAR LOCATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE CENPA-CAD COMPLEX WITH CENPI; CENPL; CENPO; CENPP; CENPQ; CENPR AND CENPS.
  8. "KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates."
    Cheeseman I.M., Hori T., Fukagawa T., Desai A.
    Mol. Biol. Cell 19:587-594(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "CENP-K and CENP-H may form coiled-coils in the kinetochores."
    Qiu S., Wang J., Yu C., He D.
    Sci. China, Ser. C, Life Sci. 52:352-359(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CENPH.

Entry informationi

Entry nameiCENPK_HUMAN
AccessioniPrimary (citable) accession number: Q9BS16
Secondary accession number(s): Q9H4L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.