Q9BRX8 (F213A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Redox-regulatory protein FAM213A

Alternative name(s):
Peroxiredoxin-like 2 activated in M-CSF stimulated monocytes
Short name=Protein PAMM

Gene names

Name:	FAM213A
Synonyms:	C10orf58, PAMM
ORF Names:	PRO2290, PSEC0139, UNQ611/PRO1198

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in redox regulation of the cell. Acts as an antioxidant. Inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. May affect bone resorption and help to maintain bone mass. Ref.8
Subcellular location	Cytoplasm Ref.8.
Tissue specificity	Expressed in CSF1 and TNFSF11-stimulated CD14+ peripheral blood mononuclear cells (PBMCs). Ref.8
Induction	By CSF1 in peripheral blood mononuclear cells (PBMCs). This induction is reduced in the presence of TNFSF11.
Miscellaneous	The active site cysteines correspond to the redox-active cysteines of peroxiredoxins.
Sequence similarities	Belongs to the peroxiredoxin-like FAM213 family. FAM213A subfamily.
Sequence caution	The sequence AAK55527.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG37828.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Redox-active center
Molecular function	Antioxidant
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	oxidation-reduction process Inferred from direct assay Ref.8. Source: UniProtKB regulation of osteoclast differentiation Inferred from direct assay Ref.8. Source: UniProtKB
Molecular function	antioxidant activity Inferred from direct assay Ref.8. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BRX8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BRX8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Redox-regulatory protein FAM213A		PRO_0000019550
Regions
	Region	14 – 112	99	Thioredoxin fold By similarity
Sites
	Active site	85	1	Redox-active By similarity
	Active site	88	1	Redox-active By similarity
Natural variations
	Alternative sequence	1 – 11	11	Missing in isoform 2.		VSP_042229
Experimental info
	Mutagenesis	85	1	C → G: Decrease of about 38% in antioxidant activity in TNFSF11-stimulated osteoclasts and reduced inhibition of TNFSF11-induced osteoclast formation. Ref.8
	Mutagenesis	88	1	C → G: Decrease of about 125% in antioxidant activity in TNFSF11-stimulated osteoclasts and reduced inhibition of TNFSF11-induced osteoclast formation. Ref.8
	Sequence conflict	201	1	K → N in BAC11108. Ref.2
	Sequence conflict	206	1	K → R in BAC11627. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 23, 2004. Version 3. Checksum: 9ECE72DEF6C338D7	FASTA	229	25,764
	10 20 30 40 50 60 MSFLQDPSFF TMGMWSIGAG ALGAAALALL LANTDVFLSK PQKAALEYLE DIDLKTLEKE 70 80 90 100 110 120 PRTFKAKELW EKNGAVIMAV RRPGCFLCRE EAADLSSLKS MLDQLGVPLY AVVKEHIRTE 130 140 150 160 170 180 VKDFQPYFKG EIFLDEKKKF YGPQRRKMMF MGFIRLGVWY NFFRAWNGGF SGNLEGEGFI 190 200 210 220 LGGVFVVGSG KQGILLEHRE KEFGDKVNLL SVLEAAKMIK PQTLASEKK
	Isoform 2 [UniParc]. Checksum: C07A4C051DD19488	FASTA	218	24,463
	10 20 30 40 50 60 MGMWSIGAGA LGAAALALLL ANTDVFLSKP QKAALEYLED IDLKTLEKEP RTFKAKELWE 70 80 90 100 110 120 KNGAVIMAVR RPGCFLCREE AADLSSLKSM LDQLGVPLYA VVKEHIRTEV KDFQPYFKGE 130 140 150 160 170 180 IFLDEKKKFY GPQRRKMMFM GFIRLGVWYN FFRAWNGGFS GNLEGEGFIL GGVFVVGSGK 190 200 210 QGILLEHREK EFGDKVNLLS VLEAAKMIKP QTLASEKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo and Heart.
[3]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed: 16303743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Muscle.
[6]	"Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs." Yu Y., Zhang C., Zhou G., Wu S., Qu X., Wei H., Xing G., Dong C., Zhai Y., Wan J., Ouyang S., Li L., Zhang S., Zhou K., Zhang Y., Wu C., He F. Genome Res. 11:1392-1403(2001) [PubMed: 11483580] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-229 (ISOFORMS 1/2). Tissue: Fetal liver.
[7]	Bienvenut W.V. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 90-99; 156-164 AND 192-199, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[8]	"PAMM: a redox regulatory protein that modulates osteoclast differentiation." Xu Y., Morse L.R., da Silva R.A., Odgren P.R., Sasaki H., Stashenko P., Battaglino R.A. Antioxid. Redox Signal. 13:27-37(2010) [PubMed: 19951071] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-85 AND CYS-88.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY359059 mRNA. Translation: AAQ89418.1. AK315440 mRNA. Translation: BAG37828.1. Different initiation. AK074643 mRNA. Translation: BAC11108.1. AK075447 mRNA. Translation: BAC11627.1. AC021028 Genomic DNA. No translation available. BC005871 mRNA. Translation: AAH05871.3. AF305824 mRNA. Translation: AAK55527.1. Different initiation.
IPI	IPI00296190.
RefSeq	NP_001230707.1. NM_001243778.1. NP_001230708.1. NM_001243779.1. NP_001230709.1. NM_001243780.1. NP_001230710.1. NM_001243781.1. NP_001230711.1. NM_001243782.1. NP_115709.3. NM_032333.4.
UniGene	Hs.500333. Hs.718589.
3D structure databases
ProteinModelPortal	Q9BRX8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9BRX8.
Polymorphism databases
DMDM	73620080.
Proteomic databases
PRIDE	Q9BRX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372181; ENSP00000361254; ENSG00000122378. ENST00000372187; ENSP00000361261; ENSG00000122378. ENST00000372188; ENSP00000361262; ENSG00000122378. ENST00000394504; ENSP00000378012; ENSG00000122378.
GeneID	84293.
KEGG	hsa:84293.
UCSC	uc001kcc.2. human.
Organism-specific databases
CTD	84293.
GeneCards	GC10P082158.
HGNC	HGNC:28651. FAM213A.
HPA	HPA009025. HPA024565.
neXtProt	NX_Q9BRX8.
PharmGKB	PA134969403.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13993.
GeneTree	ENSGT00530000063724.
HOGENOM	HBG443993.
HOVERGEN	HBG060239.
InParanoid	Q9BRX8.
OMA	CFLCREE.
OrthoDB	EOG4CNQSH.
PhylomeDB	Q9BRX8.
Gene expression databases
ArrayExpress	Q9BRX8.
Bgee	Q9BRX8.
CleanEx	HS_C10orf58.
Genevestigator	Q9BRX8.
GermOnline	ENSG00000122378. Homo sapiens.
Family and domain databases
InterPro	IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
ProtoNet	Search...
Other
NextBio	73936.

Entry information

Entry name

F213A_HUMAN

Accession

Primary (citable) accession number: Q9BRX8
Secondary accession number(s): B2RD81 , Q6UW08, Q8N2K3, Q8NBK9, Q96JR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.