Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA replication complex GINS protein PSF3

Gene

GINS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciZFISH:ENSG00000181938-MONOMER.
ReactomeiR-HSA-176974. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein PSF3
Alternative name(s):
GINS complex subunit 3
Gene namesi
Name:GINS3
Synonyms:PSF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:25851. GINS3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi64785.
OpenTargetsiENSG00000181938.
PharmGKBiPA145008327.

Polymorphism and mutation databases

BioMutaiGINS3.
DMDMi74732939.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003276151 – 216DNA replication complex GINS protein PSF3Add BLAST216

Proteomic databases

EPDiQ9BRX5.
MaxQBiQ9BRX5.
PeptideAtlasiQ9BRX5.
PRIDEiQ9BRX5.

PTM databases

iPTMnetiQ9BRX5.
PhosphoSitePlusiQ9BRX5.

Expressioni

Gene expression databases

BgeeiENSG00000181938.
CleanExiHS_GINS3.
ExpressionAtlasiQ9BRX5. baseline and differential.
GenevisibleiQ9BRX5. HS.

Organism-specific databases

HPAiHPA041216.
HPA048209.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS2. GINS complex interacts with DNA primase in vitro.5 Publications

Protein-protein interaction databases

BioGridi122295. 37 interactors.
DIPiDIP-29333N.
IntActiQ9BRX5. 3 interactors.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Helixi22 – 27Combined sources6
Beta strandi30 – 38Combined sources9
Beta strandi40 – 42Combined sources3
Helixi44 – 46Combined sources3
Helixi55 – 57Combined sources3
Beta strandi65 – 69Combined sources5
Helixi70 – 76Combined sources7
Beta strandi79 – 86Combined sources8
Helixi90 – 92Combined sources3
Helixi94 – 102Combined sources9
Helixi104 – 106Combined sources3
Turni109 – 111Combined sources3
Helixi116 – 122Combined sources7
Helixi123 – 126Combined sources4
Helixi131 – 153Combined sources23
Turni154 – 157Combined sources4
Helixi162 – 165Combined sources4
Helixi170 – 190Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E9XX-ray2.30C/G1-216[»]
2EHOX-ray3.00D/H/L1-216[»]
2Q9QX-ray2.36D/H2-216[»]
ProteinModelPortaliQ9BRX5.
SMRiQ9BRX5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BRX5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 16Not essential for folding and stability of GINS complex, but may regulate accessibility to the central complex poreAdd BLAST16

Sequence similaritiesi

Belongs to the GINS3/PSF3 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000001622.
HOGENOMiHOG000007879.
HOVERGENiHBG057574.
InParanoidiQ9BRX5.
KOiK10734.
OMAiYYYGLGS.
OrthoDBiEOG091G0QNW.
PhylomeDBiQ9BRX5.
TreeFamiTF314626.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR010492. GINS_Psf3.
[Graphical view]
PANTHERiPTHR22768. PTHR22768. 1 hit.
PfamiPF05916. Sld5. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BRX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER
60 70 80 90 100
SAGAETDNAV PQGSKLELPL WLAKGLFDNK RRILSVELPK IYQEGWRTVF
110 120 130 140 150
SADPNVVDLH KMGPHFYGFG SQLLHFDSPE NADISQSLLQ TFIGRFRRIM
160 170 180 190 200
DSSQNAYNED TSALVARLDE MERGLFQTGQ KGLNDFQCWE KGQASQITAS
210
NLVQNYKKRK FTDMED
Length:216
Mass (Da):24,535
Last modified:June 1, 2001 - v1
Checksum:iA1B264A72371C551
GO
Isoform 2 (identifier: Q9BRX5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-139: Missing.

Show »
Length:138
Mass (Da):15,655
Checksum:i50D249B01A5F66EE
GO
Isoform 3 (identifier: Q9BRX5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     63-63: G → GFALLPRLECSGVIWLTAALTSQAPEILPPQPPMWLVLQG

Note: Gene prediction based on EST data.
Show »
Length:255
Mass (Da):28,775
Checksum:i99614F114B6B6CF1
GO

Mass spectrometryi

Molecular mass is 98373±13 Da from positions 1 - 216. Determined by ESI. This is the measured mass for the GINS complex.1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03273762 – 139Missing in isoform 2. 2 PublicationsAdd BLAST78
Alternative sequenceiVSP_04669463G → GFALLPRLECSGVIWLTAAL TSQAPEILPPQPPMWLVLQG in isoform 3. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023974 mRNA. Translation: BAB14747.1.
AK315622 mRNA. Translation: BAG37990.1.
CR457283 mRNA. Translation: CAG33564.1.
AC009107 Genomic DNA. No translation available.
AC009118 Genomic DNA. No translation available.
AC084063 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW82973.1.
CH471092 Genomic DNA. Translation: EAW82976.1.
BC005879 mRNA. Translation: AAH05879.1.
BC014437 mRNA. Translation: AAH14437.1.
CCDSiCCDS10796.1. [Q9BRX5-1]
CCDS45498.1. [Q9BRX5-3]
CCDS45499.1. [Q9BRX5-2]
RefSeqiNP_001119601.1. NM_001126129.1. [Q9BRX5-3]
NP_001119602.1. NM_001126130.1. [Q9BRX5-2]
NP_073607.2. NM_022770.3. [Q9BRX5-1]
UniGeneiHs.47125.
Hs.605546.

Genome annotation databases

EnsembliENST00000318129; ENSP00000318196; ENSG00000181938. [Q9BRX5-1]
ENST00000328514; ENSP00000327449; ENSG00000181938. [Q9BRX5-2]
ENST00000426538; ENSP00000401018; ENSG00000181938. [Q9BRX5-3]
GeneIDi64785.
KEGGihsa:64785.
UCSCiuc002enh.4. human. [Q9BRX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK023974 mRNA. Translation: BAB14747.1.
AK315622 mRNA. Translation: BAG37990.1.
CR457283 mRNA. Translation: CAG33564.1.
AC009107 Genomic DNA. No translation available.
AC009118 Genomic DNA. No translation available.
AC084063 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW82973.1.
CH471092 Genomic DNA. Translation: EAW82976.1.
BC005879 mRNA. Translation: AAH05879.1.
BC014437 mRNA. Translation: AAH14437.1.
CCDSiCCDS10796.1. [Q9BRX5-1]
CCDS45498.1. [Q9BRX5-3]
CCDS45499.1. [Q9BRX5-2]
RefSeqiNP_001119601.1. NM_001126129.1. [Q9BRX5-3]
NP_001119602.1. NM_001126130.1. [Q9BRX5-2]
NP_073607.2. NM_022770.3. [Q9BRX5-1]
UniGeneiHs.47125.
Hs.605546.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E9XX-ray2.30C/G1-216[»]
2EHOX-ray3.00D/H/L1-216[»]
2Q9QX-ray2.36D/H2-216[»]
ProteinModelPortaliQ9BRX5.
SMRiQ9BRX5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122295. 37 interactors.
DIPiDIP-29333N.
IntActiQ9BRX5. 3 interactors.

PTM databases

iPTMnetiQ9BRX5.
PhosphoSitePlusiQ9BRX5.

Polymorphism and mutation databases

BioMutaiGINS3.
DMDMi74732939.

Proteomic databases

EPDiQ9BRX5.
MaxQBiQ9BRX5.
PeptideAtlasiQ9BRX5.
PRIDEiQ9BRX5.

Protocols and materials databases

DNASUi64785.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000318129; ENSP00000318196; ENSG00000181938. [Q9BRX5-1]
ENST00000328514; ENSP00000327449; ENSG00000181938. [Q9BRX5-2]
ENST00000426538; ENSP00000401018; ENSG00000181938. [Q9BRX5-3]
GeneIDi64785.
KEGGihsa:64785.
UCSCiuc002enh.4. human. [Q9BRX5-1]

Organism-specific databases

CTDi64785.
DisGeNETi64785.
GeneCardsiGINS3.
HGNCiHGNC:25851. GINS3.
HPAiHPA041216.
HPA048209.
MIMi610610. gene.
neXtProtiNX_Q9BRX5.
OpenTargetsiENSG00000181938.
PharmGKBiPA145008327.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000001622.
HOGENOMiHOG000007879.
HOVERGENiHBG057574.
InParanoidiQ9BRX5.
KOiK10734.
OMAiYYYGLGS.
OrthoDBiEOG091G0QNW.
PhylomeDBiQ9BRX5.
TreeFamiTF314626.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000181938-MONOMER.
ReactomeiR-HSA-176974. Unwinding of DNA.

Miscellaneous databases

EvolutionaryTraceiQ9BRX5.
GenomeRNAii64785.
PROiQ9BRX5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000181938.
CleanExiHS_GINS3.
ExpressionAtlasiQ9BRX5. baseline and differential.
GenevisibleiQ9BRX5. HS.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR010492. GINS_Psf3.
[Graphical view]
PANTHERiPTHR22768. PTHR22768. 1 hit.
PfamiPF05916. Sld5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSF3_HUMAN
AccessioniPrimary (citable) accession number: Q9BRX5
Secondary accession number(s): B2RDP3, E9PB21, Q9H870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.