Q9BRV8 (SIKE1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Suppressor of IKBKE 1

Alternative name(s):
Suppressor of IKK-epsilon

Gene names

Name:	SIKE1
Synonyms:	SIKE

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways. Ref.6
Subunit structure	Interacts with IKBKE and TBK1 via its coiled coil region. Interaction with TBK1 is disrupted upon viral infection or TLR3 stimulation. Ref.6
Subcellular location	Cytoplasm Ref.6.
Tissue specificity	Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level). Ref.6
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the SIKE family.
Sequence caution	The sequence CAI18824.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	innate immune response Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome focal adhesion Inferred from direct assay. Source: HPA intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRAF3IP3	Q9Y228	2	EBI-1773646,EBI-765817

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BRV8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BRV8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     53-53: Q → QVRQR

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Suppressor of IKBKE 1		PRO_0000299050
Regions
	Coiled coil	70 – 102	33	Potential
	Coiled coil	162 – 193	32	Potential
Natural variations
	Alternative sequence	53	1	Q → QVRQR in isoform 2.		VSP_027543
Experimental info
	Sequence conflict	127	1	V → I in CAH18076. Ref.2
	Sequence conflict	169	1	K → I in BAB15020. Ref.1
	Sequence conflict	191	1	L → H in BAB15020. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 4C5598612F032988	FASTA	207	23,721
	10 20 30 40 50 60 MSCTIEKILT DAKTLLERLR EHDAAAESLV DQSAALHRRV AAMREAGTAL PDQYQEDASD 70 80 90 100 110 120 MKDMSKYKPH ILLSQENTQI RDLQQENREL WISLEEHQDA LELIMSKYRK QMLQLMVAKK 130 140 150 160 170 180 AVDAEPVLKA HQSHSAEIES QIDRICEMGE VMRKAVQVDD DQFCKIQEKL AQLELENKEL 190 200 RELLSISSES LQARKENSMD TASQAIK
	Isoform 2 [UniParc]. Checksum: 555B830504AE0EB7	FASTA	211	24,261
	10 20 30 40 50 60 MSCTIEKILT DAKTLLERLR EHDAAAESLV DQSAALHRRV AAMREAGTAL PDQVRQRYQE 70 80 90 100 110 120 DASDMKDMSK YKPHILLSQE NTQIRDLQQE NRELWISLEE HQDALELIMS KYRKQMLQLM 130 140 150 160 170 180 VAKKAVDAEP VLKAHQSHSA EIESQIDRIC EMGEVMRKAV QVDDDQFCKI QEKLAQLELE 190 200 210 NKELRELLSI SSESLQARKE NSMDTASQAI K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Smooth muscle.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Endometrial adenocarcinoma.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Prostate.
[6]	"SIKE is an IKK epsilon/TBK1-associated suppressor of TLR3- and virus-triggered IRF-3 activation pathways." Huang J., Liu T., Xu L.-G., Chen D., Zhai Z., Shu H.-B. EMBO J. 24:4018-4028(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH IKBKE AND TBK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK024821 mRNA. Translation: BAB15020.1. CR749219 mRNA. Translation: CAH18076.1. AL096773 Genomic DNA. Translation: CAI18822.1. AL096773 Genomic DNA. Translation: CAI18823.1. AL096773 Genomic DNA. Translation: CAI18824.1. Sequence problems. CH471122 Genomic DNA. Translation: EAW56620.1. BC005934 mRNA. Translation: AAH05934.1.
IPI	IPI00305186. IPI00640464.
RefSeq	NP_001095866.1. NM_001102396.1. NP_079349.2. NM_025073.2.
UniGene	Hs.709277.
3D structure databases
ProteinModelPortal	Q9BRV8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BRV8. 21 interactions.
STRING	9606.ENSP00000358541.
PTM databases
PhosphoSite	Q9BRV8.
Polymorphism databases
DMDM	74752290.
Proteomic databases
PaxDb	Q9BRV8.
PRIDE	Q9BRV8.
Protocols and materials databases
DNASU	80143.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000060969; ENSP00000060969; ENSG00000052723. ENST00000369528; ENSP00000358541; ENSG00000052723.
GeneID	80143.
KEGG	hsa:80143.
UCSC	uc001efo.4. human. uc001efp.4. human.
Organism-specific databases
CTD	80143.
GeneCards	GC01M115312.
HGNC	HGNC:26119. SIKE1.
HPA	HPA024177. HPA025726.
MIM	611656. gene.
neXtProt	NX_Q9BRV8.
PharmGKB	PA165752371.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG72930.
HOGENOM	HOG000294110.
HOVERGEN	HBG055808.
KO	K12656.
OMA	QYQEDAS.
OrthoDB	EOG4BG8X5.
PhylomeDB	Q9BRV8.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
Bgee	Q9BRV8.
Genevestigator	Q9BRV8.
Family and domain databases
InterPro	IPR008555. SIKE. [Graphical view]
PANTHER	PTHR12186. PTHR12186. 1 hit.
Pfam	PF05769. DUF837. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SIKE1. human.
GenomeRNAi	80143.
NextBio	70409.
SOURCE	Search...

Entry information

Entry name

SIKE1_HUMAN

Accession

Primary (citable) accession number: Q9BRV8
Secondary accession number(s): Q5TEZ7 , Q5TEZ9, Q68DZ4, Q9H778

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.