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Protein

DNA replication complex GINS protein SLD5

Gene

GINS4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciZFISH:ENSG00000147536-MONOMER.
ReactomeiR-HSA-176974. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein SLD5
Alternative name(s):
GINS complex subunit 4
Cleaved into the following chain:
Gene namesi
Name:GINS4
Synonyms:SLD5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:28226. GINS4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000147536.
PharmGKBiPA145008337.

Polymorphism and mutation databases

BioMutaiGINS4.
DMDMi74732928.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003276201 – 223DNA replication complex GINS protein SLD5Add BLAST223
Initiator methionineiRemoved; alternateCombined sources
ChainiPRO_00004217942 – 223DNA replication complex GINS protein SLD5, N-terminally processedAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei2N-acetylthreonine; in DNA replication complex GINS protein SLD5, N-terminally processedCombined sources1
Modified residuei12PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BRT9.
MaxQBiQ9BRT9.
PaxDbiQ9BRT9.
PeptideAtlasiQ9BRT9.
PRIDEiQ9BRT9.

PTM databases

iPTMnetiQ9BRT9.
PhosphoSitePlusiQ9BRT9.

Expressioni

Inductioni

Significantly up-regulated in aggressive melanomas.1 Publication

Gene expression databases

BgeeiENSG00000147536.
CleanExiHS_GINS4.
ExpressionAtlasiQ9BRT9. baseline and differential.
GenevisibleiQ9BRT9. HS.

Organism-specific databases

HPAiHPA024663.
HPA026580.
HPA061075.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS1. GINS complex interacts with DNA primase in vitro.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GINS2Q9Y2486EBI-747500,EBI-747491
MCMBPQ9BTE32EBI-747500,EBI-749378

Protein-protein interaction databases

BioGridi124023. 40 interactors.
DIPiDIP-29334N.
IntActiQ9BRT9. 9 interactors.
MINTiMINT-1469672.
STRINGi9606.ENSP00000276533.

Structurei

Secondary structure

1223
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 39Combined sources17
Beta strandi40 – 42Combined sources3
Helixi48 – 60Combined sources13
Helixi72 – 102Combined sources31
Helixi104 – 112Combined sources9
Helixi124 – 144Combined sources21
Helixi146 – 148Combined sources3
Helixi151 – 153Combined sources3
Helixi158 – 161Combined sources4
Beta strandi170 – 177Combined sources8
Beta strandi179 – 184Combined sources6
Helixi190 – 192Combined sources3
Beta strandi194 – 198Combined sources5
Beta strandi203 – 207Combined sources5
Helixi208 – 217Combined sources10
Beta strandi218 – 223Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E9XX-ray2.30D/H1-223[»]
2EHOX-ray3.00A/E/I11-213[»]
2Q9QX-ray2.36B/F1-223[»]
ProteinModelPortaliQ9BRT9.
SMRiQ9BRT9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BRT9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 223Important for GINS complex assemblyAdd BLAST58

Sequence similaritiesi

Belongs to the GINS4/SLD5 family.Curated

Phylogenomic databases

eggNOGiKOG3176. Eukaryota.
COG5086. LUCA.
GeneTreeiENSGT00390000003246.
HOGENOMiHOG000215319.
HOVERGENiHBG059490.
InParanoidiQ9BRT9.
KOiK10735.
OMAiVSIHRME.
OrthoDBiEOG091G0ULR.
PhylomeDBiQ9BRT9.
TreeFamiTF105863.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR008591. GINS_Sld5.
IPR031633. SLD5_C.
[Graphical view]
PANTHERiPTHR21206. PTHR21206. 1 hit.
PfamiPF05916. Sld5. 1 hit.
PF16922. SLD5_C. 1 hit.
[Graphical view]
PIRSFiPIRSF007764. Sld5. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BRT9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEEVDFLGQ DSDGGSEEVV LTPAELIERL EQAWMNEKFA PELLESKPEI
60 70 80 90 100
VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI
110 120 130 140 150
EKFFPHVLEK EKTRPEGEPS SLSPEELAFA REFMANTESY LKNVALKHMP
160 170 180 190 200
PNLQKVDLFR AVPKPDLDSY VFLRVRERQE NILVEPDTDE QRDYVIDLEK
210 220
GSQHLIRYKT IAPLVASGAV QLI
Length:223
Mass (Da):26,047
Last modified:June 1, 2001 - v1
Checksum:i8FAB619A21968BB9
GO
Isoform 2 (identifier: Q9BRT9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-100: I → V
     101-223: Missing.

Show »
Length:100
Mass (Da):11,844
Checksum:i2E8F496F2DC656B9
GO

Mass spectrometryi

Molecular mass is 98373±13 Da from positions 1 - 223. Determined by ESI. This is the measured mass for the GINS complex.1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032738100I → V in isoform 2. 1 Publication1
Alternative sequenceiVSP_032739101 – 223Missing in isoform 2. 1 PublicationAdd BLAST123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095334 mRNA. Translation: BAG53029.1.
AK313373 mRNA. Translation: BAG36172.1.
CH471080 Genomic DNA. Translation: EAW63256.1.
CH471080 Genomic DNA. Translation: EAW63257.1.
BC005995 mRNA. Translation: AAH05995.1.
BC027454 mRNA. Translation: AAH27454.1.
CCDSiCCDS6116.1. [Q9BRT9-1]
RefSeqiNP_115712.1. NM_032336.2. [Q9BRT9-1]
XP_005273716.1. XM_005273659.3. [Q9BRT9-1]
UniGeneiHs.656996.

Genome annotation databases

EnsembliENST00000276533; ENSP00000276533; ENSG00000147536. [Q9BRT9-1]
ENST00000518671; ENSP00000428754; ENSG00000147536. [Q9BRT9-1]
ENST00000520710; ENSP00000429581; ENSG00000147536. [Q9BRT9-2]
GeneIDi84296.
KEGGihsa:84296.
UCSCiuc003xnx.3. human. [Q9BRT9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095334 mRNA. Translation: BAG53029.1.
AK313373 mRNA. Translation: BAG36172.1.
CH471080 Genomic DNA. Translation: EAW63256.1.
CH471080 Genomic DNA. Translation: EAW63257.1.
BC005995 mRNA. Translation: AAH05995.1.
BC027454 mRNA. Translation: AAH27454.1.
CCDSiCCDS6116.1. [Q9BRT9-1]
RefSeqiNP_115712.1. NM_032336.2. [Q9BRT9-1]
XP_005273716.1. XM_005273659.3. [Q9BRT9-1]
UniGeneiHs.656996.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E9XX-ray2.30D/H1-223[»]
2EHOX-ray3.00A/E/I11-213[»]
2Q9QX-ray2.36B/F1-223[»]
ProteinModelPortaliQ9BRT9.
SMRiQ9BRT9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124023. 40 interactors.
DIPiDIP-29334N.
IntActiQ9BRT9. 9 interactors.
MINTiMINT-1469672.
STRINGi9606.ENSP00000276533.

PTM databases

iPTMnetiQ9BRT9.
PhosphoSitePlusiQ9BRT9.

Polymorphism and mutation databases

BioMutaiGINS4.
DMDMi74732928.

Proteomic databases

EPDiQ9BRT9.
MaxQBiQ9BRT9.
PaxDbiQ9BRT9.
PeptideAtlasiQ9BRT9.
PRIDEiQ9BRT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276533; ENSP00000276533; ENSG00000147536. [Q9BRT9-1]
ENST00000518671; ENSP00000428754; ENSG00000147536. [Q9BRT9-1]
ENST00000520710; ENSP00000429581; ENSG00000147536. [Q9BRT9-2]
GeneIDi84296.
KEGGihsa:84296.
UCSCiuc003xnx.3. human. [Q9BRT9-1]

Organism-specific databases

CTDi84296.
GeneCardsiGINS4.
HGNCiHGNC:28226. GINS4.
HPAiHPA024663.
HPA026580.
HPA061075.
MIMi610611. gene.
neXtProtiNX_Q9BRT9.
OpenTargetsiENSG00000147536.
PharmGKBiPA145008337.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3176. Eukaryota.
COG5086. LUCA.
GeneTreeiENSGT00390000003246.
HOGENOMiHOG000215319.
HOVERGENiHBG059490.
InParanoidiQ9BRT9.
KOiK10735.
OMAiVSIHRME.
OrthoDBiEOG091G0ULR.
PhylomeDBiQ9BRT9.
TreeFamiTF105863.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000147536-MONOMER.
ReactomeiR-HSA-176974. Unwinding of DNA.

Miscellaneous databases

ChiTaRSiGINS4. human.
EvolutionaryTraceiQ9BRT9.
GenomeRNAii84296.
PROiQ9BRT9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000147536.
CleanExiHS_GINS4.
ExpressionAtlasiQ9BRT9. baseline and differential.
GenevisibleiQ9BRT9. HS.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR008591. GINS_Sld5.
IPR031633. SLD5_C.
[Graphical view]
PANTHERiPTHR21206. PTHR21206. 1 hit.
PfamiPF05916. Sld5. 1 hit.
PF16922. SLD5_C. 1 hit.
[Graphical view]
PIRSFiPIRSF007764. Sld5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSLD5_HUMAN
AccessioniPrimary (citable) accession number: Q9BRT9
Secondary accession number(s): B2R8H5, D3DSY0, Q8N648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.