Q9BRT3 (MIEN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Migration and invasion enhancer 1

Alternative name(s):
HBV X-transactivated gene 4 protein
HBV XAg-transactivated protein 4
Protein C35

Gene names

Name:	MIEN1
Synonyms:	C17orf37, RDX12, XTP4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process. Ref.5 Ref.7
Subunit structure	Interacts with GPX1 By similarity.
Subcellular location	Cytoplasm › cytosol. Cell membrane; Lipid-anchor; Cytoplasmic side. Note: Concentrates at the leading edge of migrating cells. Localizes outside membrane raft regions. Ref.1 Ref.5 Ref.7
Tissue specificity	Among normal tissues, present only in Leydig cells. Strongly up-regulated in breast cancers and in brain cancer distant metastasis (at protein level). Up-regulated in prostate cancer cells and in the higher grades of prostate adenocarcinoma (at protein level). Ref.1 Ref.5
Post-translational modification	Isoprenylation facilitates association with the plasma membrane and enhances the migratory phenotype of cells by inducing increased filopodia formation.
Sequence similarities	Belongs to the SelWTH family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Redox-active center
PTM	Disulfide bond Lipoprotein Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell redox homeostasis Inferred from electronic annotation. Source: InterPro negative regulation of apoptotic process Inferred from direct assay PubMed 21068479. Source: UniProtKB positive regulation of cell migration Inferred from direct assay Ref.7. Source: UniProtKB positive regulation of filopodium assembly Inferred from direct assay Ref.7. Source: UniProtKB
Cellular_component	cytosol Inferred from direct assay Ref.7. Source: UniProtKB intrinsic to internal side of plasma membrane Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	selenium binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 112

112

Migration and invasion enhancer 1

PRO_0000265099

Propeptide

113 – 115

3

Removed in mature form Probable

PRO_0000396008

Amino acid modifications

Lipidation

112

1

S-geranylgeranyl cysteine Ref.1 Ref.5 Ref.7

Disulfide bond

30 ↔ 33

Redox-active Ref.8

Experimental info

Mutagenesis

112 – 115

4

Missing: No effect on subcellular location. Low protein abundance, suggesting that stability is affected. Ref.7

Mutagenesis

112

1

C → S: Abolishes prenylation. Predominantly cysolic with little plasma membrane-associated expression. Reduces cell migration by affecting filopodia formation. Ref.7

Secondary structure

1

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115

Helix Strand Turn

Details...

Beta strand

26 – 29

4

Turn

31 – 34

4

Helix

36 – 46

11

Turn

47 – 49

3

Beta strand

51 – 53

3

Beta strand

56 – 59

4

Beta strand

61 – 63

3

Beta strand

65 – 68

4

Helix

77 – 80

4

Turn

86 – 90

5

Helix

91 – 96

6

Turn

97 – 99

3

Sequences

	Sequence		Length	Mass (Da)
	Q9BRT3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 5D8B911C0F23DDC1	FASTA	115	12,403
	10 20 30 40 50 60 MSGEPGQTSV APPPEEVEPG SGVRIVVEYC EPCGFEATYL ELASAVKEQY PGIEIESRLG 70 80 90 100 110 GTGAFEIEIN GQLVFSKLEN GGFPYEKDLI EAIRRASNGE TLEKITNSRP PCVIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"C35 (C17orf37) is a novel tumor biomarker abundantly expressed in breast cancer." Evans E.E., Henn A.D., Jonason A., Paris M.J., Schiffhauer L.M., Borrello M.A., Smith E.S., Sahasrabudhe D.M., Zauderer M. Mol. Cancer Ther. 5:2919-2930(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, PROBABLE ISOPRENYLATION.
[2]	"Cloning and identification of human gene 4 transactivated by hepatitis B virus X antigen." Liu Y., Cheng J., Lu Y., Wang G., Zhang L., Chen J., Li L. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Novel gene C17orf37 in 17q12 amplicon promotes migration and invasion of prostate cancer cells." Dasgupta S., Wasson L.M., Rauniyar N., Prokai L., Borejdo J., Vishwanatha J.K. Oncogene 28:2860-2872(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, PROBABLE ISOPRENYLATION AT CYS-112.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Prenylated c17orf37 induces filopodia formation to promote cell migration and metastasis." Dasgupta S., Cushman I., Kpetemey M., Casey P.J., Vishwanatha J.K. J. Biol. Chem. 286:25935-25946(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, ISOPRENYLATION AT CYS-112, MUTAGENESIS OF CYS-112 AND 112-CYS--LEU-115.
[8]	"Solution structure of the oncogenic MIEN1 protein reveals a thioredoxin-like fold with a redox-active motif." Hsu C.H., Shen T.L., Chang C.F., Chang Y.Y., Huang L.Y. PLoS ONE 7:E52292-E52292(2012) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY508814 mRNA. Translation: AAR92035.1.
AF490253 mRNA. Translation: AAO85461.1.
AC079199 Genomic DNA. No translation available.
BC006006 mRNA. Translation: AAH06006.1.

IPI

IPI00878073.

RefSeq

NP_115715.3. NM_032339.3.

UniGene

Hs.333526.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LJK	NMR	-	A	1-115	[»]

ProteinModelPortal

Q9BRT3.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000377778.

PTM databases

PhosphoSite

Q9BRT3.

Polymorphism databases

DMDM

74732925.

Proteomic databases

PaxDb

Q9BRT3.

PRIDE

Q9BRT3.

Protocols and materials databases

DNASU

84299.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000394231; ENSP00000377778; ENSG00000141741.

GeneID

84299.

KEGG

hsa:84299.

UCSC

uc002hsq.3. human.

Organism-specific databases

CTD

84299.

GeneCards

GC17M037886.

H-InvDB

HIX0013783.

HGNC

HGNC:28230. MIEN1.

MIM

611802. gene.

neXtProt

NX_Q9BRT3.

PharmGKB

PA134947080.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG85235.

HOGENOM

HOG000275576.

HOVERGEN

HBG059755.

InParanoid

Q9BRT3.

KO

K07401.

OMA

RPPCIIL.

OrthoDB

EOG4X3H2V.

PhylomeDB

Q9BRT3.

Gene expression databases

Bgee

Q9BRT3.

CleanEx

HS_C17orf37.

Genevestigator

Q9BRT3.

GermOnline

ENSG00000141741. Homo sapiens.

Family and domain databases

Gene3D

3.40.30.10. 1 hit.

InterPro

IPR011893. Selenoprotein_Rdx-typ.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Pfam

PF10262. Rdx. 1 hit.
[Graphical view]

SUPFAM

SSF52833. Thiordxn-like_fd. 1 hit.

TIGRFAMs

TIGR02174. CXXU_selWTH. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

MIEN1. human.

GenomeRNAi

84299.

NextBio

73960.

SOURCE

Search...

Entry information

Entry name

MIEN1_HUMAN

Accession

Primary (citable) accession number: Q9BRT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.