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Q9BRT3

- MIEN1_HUMAN

UniProt

Q9BRT3 - MIEN1_HUMAN

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Protein
Migration and invasion enhancer 1
Gene
MIEN1, C17orf37, RDX12, XTP4
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process.2 Publications

GO - Molecular functioni

  1. selenium binding Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. cell redox homeostasis Source: InterPro
  3. negative regulation of apoptotic process Source: UniProtKB
  4. positive regulation of cell migration Source: UniProtKB
  5. positive regulation of filopodium assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Migration and invasion enhancer 1
Alternative name(s):
HBV X-transactivated gene 4 protein
HBV XAg-transactivated protein 4
Protein C35
Gene namesi
Name:MIEN1
Synonyms:C17orf37, RDX12, XTP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:28230. MIEN1.

Subcellular locationi

Cytoplasmcytosol. Cell membrane; Lipid-anchor; Cytoplasmic side
Note: Concentrates at the leading edge of migrating cells. Localizes outside membrane raft regions.3 Publications

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. extracellular vesicular exosome Source: UniProt
  3. intrinsic component of the cytoplasmic side of the plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi112 – 1154Missing: No effect on subcellular location. Low protein abundance, suggesting that stability is affected. 1 Publication
Mutagenesisi112 – 1121C → S: Abolishes prenylation. Predominantly cysolic with little plasma membrane-associated expression. Reduces cell migration by affecting filopodia formation. 1 Publication

Organism-specific databases

PharmGKBiPA134947080.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 112111Migration and invasion enhancer 1
PRO_0000265099Add
BLAST
Propeptidei113 – 1153Removed in mature form Inferred
PRO_0000396008

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Disulfide bondi30 ↔ 33Redox-active1 Publication
Lipidationi112 – 1121S-geranylgeranyl cysteine3 Publications

Post-translational modificationi

Isoprenylation facilitates association with the plasma membrane and enhances the migratory phenotype of cells by inducing increased filopodia formation.

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Prenylation

Proteomic databases

MaxQBiQ9BRT3.
PaxDbiQ9BRT3.
PRIDEiQ9BRT3.

PTM databases

PhosphoSiteiQ9BRT3.

Expressioni

Tissue specificityi

Among normal tissues, present only in Leydig cells. Strongly up-regulated in breast cancers and in brain cancer distant metastasis (at protein level). Up-regulated in prostate cancer cells and in the higher grades of prostate adenocarcinoma (at protein level).2 Publications

Gene expression databases

ArrayExpressiQ9BRT3.
BgeeiQ9BRT3.
CleanExiHS_C17orf37.
GenevestigatoriQ9BRT3.

Organism-specific databases

HPAiHPA056650.
HPA061344.

Interactioni

Subunit structurei

Interacts with GPX1 By similarity.

Protein-protein interaction databases

STRINGi9606.ENSP00000377778.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 294
Turni31 – 344
Helixi36 – 4611
Turni47 – 493
Beta strandi51 – 533
Beta strandi56 – 594
Beta strandi61 – 633
Beta strandi65 – 684
Helixi77 – 804
Turni86 – 905
Helixi91 – 966
Turni97 – 993

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LJKNMR-A1-115[»]
ProteinModelPortaliQ9BRT3.
SMRiQ9BRT3. Positions 1-115.

Family & Domainsi

Sequence similaritiesi

Belongs to the SelWTH family.

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiNOG85235.
HOGENOMiHOG000275576.
HOVERGENiHBG059755.
InParanoidiQ9BRT3.
KOiK07401.
OMAiMYTSSAS.
OrthoDBiEOG7SR4Q5.
PhylomeDBiQ9BRT3.
TreeFamiTF326627.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR011893. Selenoprotein_Rdx-typ.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF10262. Rdx. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR02174. CXXU_selWTH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BRT3-1 [UniParc]FASTAAdd to Basket

« Hide

MSGEPGQTSV APPPEEVEPG SGVRIVVEYC EPCGFEATYL ELASAVKEQY    50
PGIEIESRLG GTGAFEIEIN GQLVFSKLEN GGFPYEKDLI EAIRRASNGE 100
TLEKITNSRP PCVIL 115
Length:115
Mass (Da):12,403
Last modified:June 1, 2001 - v1
Checksum:i5D8B911C0F23DDC1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY508814 mRNA. Translation: AAR92035.1.
AF490253 mRNA. Translation: AAO85461.1.
AC079199 Genomic DNA. No translation available.
BC006006 mRNA. Translation: AAH06006.1.
CCDSiCCDS11344.1.
RefSeqiNP_115715.3. NM_032339.3.
UniGeneiHs.333526.

Genome annotation databases

EnsembliENST00000394231; ENSP00000377778; ENSG00000141741.
GeneIDi84299.
KEGGihsa:84299.
UCSCiuc002hsq.3. human.

Polymorphism databases

DMDMi74732925.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY508814 mRNA. Translation: AAR92035.1 .
AF490253 mRNA. Translation: AAO85461.1 .
AC079199 Genomic DNA. No translation available.
BC006006 mRNA. Translation: AAH06006.1 .
CCDSi CCDS11344.1.
RefSeqi NP_115715.3. NM_032339.3.
UniGenei Hs.333526.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LJK NMR - A 1-115 [» ]
ProteinModelPortali Q9BRT3.
SMRi Q9BRT3. Positions 1-115.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000377778.

PTM databases

PhosphoSitei Q9BRT3.

Polymorphism databases

DMDMi 74732925.

Proteomic databases

MaxQBi Q9BRT3.
PaxDbi Q9BRT3.
PRIDEi Q9BRT3.

Protocols and materials databases

DNASUi 84299.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000394231 ; ENSP00000377778 ; ENSG00000141741 .
GeneIDi 84299.
KEGGi hsa:84299.
UCSCi uc002hsq.3. human.

Organism-specific databases

CTDi 84299.
GeneCardsi GC17M037886.
H-InvDB HIX0013783.
HGNCi HGNC:28230. MIEN1.
HPAi HPA056650.
HPA061344.
MIMi 611802. gene.
neXtProti NX_Q9BRT3.
PharmGKBi PA134947080.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG85235.
HOGENOMi HOG000275576.
HOVERGENi HBG059755.
InParanoidi Q9BRT3.
KOi K07401.
OMAi MYTSSAS.
OrthoDBi EOG7SR4Q5.
PhylomeDBi Q9BRT3.
TreeFami TF326627.

Miscellaneous databases

ChiTaRSi MIEN1. human.
GenomeRNAii 84299.
NextBioi 73960.
PROi Q9BRT3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9BRT3.
Bgeei Q9BRT3.
CleanExi HS_C17orf37.
Genevestigatori Q9BRT3.

Family and domain databases

Gene3Di 3.40.30.10. 1 hit.
InterProi IPR011893. Selenoprotein_Rdx-typ.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF10262. Rdx. 1 hit.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 1 hit.
TIGRFAMsi TIGR02174. CXXU_selWTH. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ISOPRENYLATION.
  2. "Cloning and identification of human gene 4 transactivated by hepatitis B virus X antigen."
    Liu Y., Cheng J., Lu Y., Wang G., Zhang L., Chen J., Li L.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  6. "Novel gene C17orf37 in 17q12 amplicon promotes migration and invasion of prostate cancer cells."
    Dasgupta S., Wasson L.M., Rauniyar N., Prokai L., Borejdo J., Vishwanatha J.K.
    Oncogene 28:2860-2872(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ISOPRENYLATION AT CYS-112.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Prenylated c17orf37 induces filopodia formation to promote cell migration and metastasis."
    Dasgupta S., Cushman I., Kpetemey M., Casey P.J., Vishwanatha J.K.
    J. Biol. Chem. 286:25935-25946(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ISOPRENYLATION AT CYS-112, MUTAGENESIS OF CYS-112 AND 112-CYS--LEU-115.
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Solution structure of the oncogenic MIEN1 protein reveals a thioredoxin-like fold with a redox-active motif."
    Hsu C.H., Shen T.L., Chang C.F., Chang Y.Y., Huang L.Y.
    PLoS ONE 7:E52292-E52292(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, DISULFIDE BOND.

Entry informationi

Entry nameiMIEN1_HUMAN
AccessioniPrimary (citable) accession number: Q9BRT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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