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Protein

Serine/threonine-protein kinase RIO1

Gene

RIOK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208ATPCombined sources1 Publication1
Binding sitei278ATP; via carbonyl oxygenCombined sources1 Publication1
Binding sitei280ATP; via amide nitrogenCombined sources1 Publication1
Active sitei324Proton acceptor1 Publication1 Publication1
Metal bindingi329MagnesiumCombined sources1 Publication1
Active sitei3414-aspartylphosphate intermediateCombined sources1 Publication1
Metal bindingi341MagnesiumCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Kinase, Serine/threonine-protein kinase, Transferase
Biological processRibosome biogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RIO1 (EC:2.7.11.1, EC:3.6.3.-)
Alternative name(s):
RIO kinase 1
Gene namesi
Name:RIOK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:18656. RIOK1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi324D → A: Abolishes autophosphorylation activity; enhances association with pre-40S ribosomal subunits; inhibits processing of 18S-E pre-rRNA to the mature 18S rRNA. 1 Publication1

Organism-specific databases

DisGeNETi83732.
OpenTargetsiENSG00000124784.
PharmGKBiPA134928236.

Chemistry databases

ChEMBLiCHEMBL5975.
GuidetoPHARMACOLOGYi2186.

Polymorphism and mutation databases

BioMutaiRIOK1.
DMDMi56404949.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135261 – 568Serine/threonine-protein kinase RIO1Add BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21PhosphoserineCombined sources1
Modified residuei22PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BRS2.
MaxQBiQ9BRS2.
PaxDbiQ9BRS2.
PeptideAtlasiQ9BRS2.
PRIDEiQ9BRS2.

PTM databases

iPTMnetiQ9BRS2.
PhosphoSitePlusiQ9BRS2.

Expressioni

Gene expression databases

BgeeiENSG00000124784.
CleanExiHS_RIOK1.
ExpressionAtlasiQ9BRS2. baseline and differential.
GenevisibleiQ9BRS2. HS.

Organism-specific databases

HPAiHPA017866.
HPA051446.

Interactioni

Subunit structurei

Associates with the precursor of the 40S ribosome subunit. Interacts with PRMT5 and WDR77.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PRMT5O147444EBI-7307838,EBI-351098

Protein-protein interaction databases

BioGridi123743. 41 interactors.
IntActiQ9BRS2. 11 interactors.
MINTiMINT-1630868.
STRINGi9606.ENSP00000369162.

Chemistry databases

BindingDBiQ9BRS2.

Structurei

Secondary structure

1568
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi166 – 175Combined sources10
Beta strandi181 – 188Combined sources8
Beta strandi190 – 198Combined sources9
Beta strandi204 – 210Combined sources7
Helixi246 – 259Combined sources14
Beta strandi267 – 271Combined sources5
Beta strandi274 – 278Combined sources5
Helixi290 – 292Combined sources3
Helixi297 – 316Combined sources20
Helixi327 – 329Combined sources3
Beta strandi330 – 333Combined sources4
Beta strandi336 – 339Combined sources4
Helixi353 – 369Combined sources17
Helixi378 – 386Combined sources9
Helixi395 – 408Combined sources14
Helixi411 – 428Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OTPX-ray2.70A143-494[»]
ProteinModelPortaliQ9BRS2.
SMRiQ9BRS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 479Protein kinaseAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2270. Eukaryota.
COG1718. LUCA.
GeneTreeiENSGT00390000004370.
HOGENOMiHOG000166501.
HOVERGENiHBG056997.
InParanoidiQ9BRS2.
KOiK07178.
OMAiRKEKMGS.
OrthoDBiEOG090705QY.
PhylomeDBiQ9BRS2.
TreeFamiTF105831.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
IPR017407. Ser/Thr_kinase_Rio1.
PIRSFiPIRSF038147. Ser/Thr_PK_RIO1. 1 hit.
SMARTiView protein in SMART
SM00090. RIO. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01245. RIO1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BRS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYRRLLMSR VVPGQFDDAD SSDSENRDLK TVKEKDDILF EDLQDNVNEN
60 70 80 90 100
GEGEIEDEEE EGYDDDDDDW DWDEGVGKLA KGYVWNGGSN PQANRQTSDS
110 120 130 140 150
SSAKMSTPAD KVLRKFENKI NLDKLNVTDS VINKVTEKSR QKEADMYRIK
160 170 180 190 200
DKADRATVEQ VLDPRTRMIL FKMLTRGIIT EINGCISTGK EANVYHASTA
210 220 230 240 250
NGESRAIKIY KTSILVFKDR DKYVSGEFRF RHGYCKGNPR KMVKTWAEKE
260 270 280 290 300
MRNLIRLNTA EIPCPEPIML RSHVLVMSFI GKDDMPAPLL KNVQLSESKA
310 320 330 340 350
RELYLQVIQY MRRMYQDARL VHADLSEFNM LYHGGGVYII DVSQSVEHDH
360 370 380 390 400
PHALEFLRKD CANVNDFFMR HSVAVMTVRE LFEFVTDPSI THENMDAYLS
410 420 430 440 450
KAMEIASQRT KEERSSQDHV DEEVFKRAYI PRTLNEVKNY ERDMDIIMKL
460 470 480 490 500
KEEDMAMNAQ QDNILYQTVT GLKKDLSGVQ KVPALLENQV EERTCSDSED
510 520 530 540 550
IGSSECSDTD SEEQGDHARP KKHTTDPDID KKERKKMVKE AQREKRKNKI
560
PKHVKKRKEK TAKTKKGK
Length:568
Mass (Da):65,583
Last modified:March 1, 2004 - v2
Checksum:i5730BFC3798F5190
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061777375V → I. Corresponds to variant dbSNP:rs56067778Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK054568 mRNA. Translation: BAB70761.1.
AK314467 mRNA. Translation: BAG37075.1.
CH471087 Genomic DNA. Translation: EAW55210.1.
BC006104 mRNA. Translation: AAH06104.2.
AL834277 mRNA. Translation: CAD38952.1.
CCDSiCCDS4500.1.
RefSeqiNP_113668.2. NM_031480.2.
UniGeneiHs.437474.

Genome annotation databases

EnsembliENST00000379834; ENSP00000369162; ENSG00000124784.
GeneIDi83732.
KEGGihsa:83732.
UCSCiuc003mxn.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK054568 mRNA. Translation: BAB70761.1.
AK314467 mRNA. Translation: BAG37075.1.
CH471087 Genomic DNA. Translation: EAW55210.1.
BC006104 mRNA. Translation: AAH06104.2.
AL834277 mRNA. Translation: CAD38952.1.
CCDSiCCDS4500.1.
RefSeqiNP_113668.2. NM_031480.2.
UniGeneiHs.437474.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OTPX-ray2.70A143-494[»]
ProteinModelPortaliQ9BRS2.
SMRiQ9BRS2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123743. 41 interactors.
IntActiQ9BRS2. 11 interactors.
MINTiMINT-1630868.
STRINGi9606.ENSP00000369162.

Chemistry databases

BindingDBiQ9BRS2.
ChEMBLiCHEMBL5975.
GuidetoPHARMACOLOGYi2186.

PTM databases

iPTMnetiQ9BRS2.
PhosphoSitePlusiQ9BRS2.

Polymorphism and mutation databases

BioMutaiRIOK1.
DMDMi56404949.

Proteomic databases

EPDiQ9BRS2.
MaxQBiQ9BRS2.
PaxDbiQ9BRS2.
PeptideAtlasiQ9BRS2.
PRIDEiQ9BRS2.

Protocols and materials databases

DNASUi83732.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379834; ENSP00000369162; ENSG00000124784.
GeneIDi83732.
KEGGihsa:83732.
UCSCiuc003mxn.4. human.

Organism-specific databases

CTDi83732.
DisGeNETi83732.
GeneCardsiRIOK1.
HGNCiHGNC:18656. RIOK1.
HPAiHPA017866.
HPA051446.
neXtProtiNX_Q9BRS2.
OpenTargetsiENSG00000124784.
PharmGKBiPA134928236.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2270. Eukaryota.
COG1718. LUCA.
GeneTreeiENSGT00390000004370.
HOGENOMiHOG000166501.
HOVERGENiHBG056997.
InParanoidiQ9BRS2.
KOiK07178.
OMAiRKEKMGS.
OrthoDBiEOG090705QY.
PhylomeDBiQ9BRS2.
TreeFamiTF105831.

Enzyme and pathway databases

ReactomeiR-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

ChiTaRSiRIOK1. human.
GenomeRNAii83732.
PROiQ9BRS2.

Gene expression databases

BgeeiENSG00000124784.
CleanExiHS_RIOK1.
ExpressionAtlasiQ9BRS2. baseline and differential.
GenevisibleiQ9BRS2. HS.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
IPR017407. Ser/Thr_kinase_Rio1.
PIRSFiPIRSF038147. Ser/Thr_PK_RIO1. 1 hit.
SMARTiView protein in SMART
SM00090. RIO. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01245. RIO1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIOK1_HUMAN
AccessioniPrimary (citable) accession number: Q9BRS2
Secondary accession number(s): B2RB28, Q8NDC8, Q96NV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2004
Last modified: February 15, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.