Q9BRR9 (RHG09_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho GTPase-activating protein 9

Alternative name(s):
Rho-type GTPase-activating protein 9

Gene names

Name:

ARHGAP9

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. Ref.1
Subunit structure	Interacts with FASLG. Ref.6
Tissue specificity	Predominantly expressed in peripheral blood leukocytes, spleen, and thymus. Ref.1
Sequence similarities	Contains 1 PH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain. Contains 1 WW domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	SH3 domain
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	GTPase activator activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BRR9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BRR9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     438-456: Missing.

Isoform 3 (identifier: Q9BRR9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-184: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 750

750

Rho GTPase-activating protein 9

PRO_0000056712

Regions

Domain

22 – 88

67

SH3

Domain

213 – 247

35

WW

Domain

322 – 435

114

PH

Domain

542 – 749

208

Rho-GAP

Amino acid modifications

Modified residue

475

1

Phosphoserine Ref.5 Ref.7

Natural variations

Alternative sequence

1 – 184

184

Missing in isoform 3.

VSP_010340

Alternative sequence

438 – 456

19

Missing in isoform 2.

VSP_010325

Natural variant

50

1

R → G.
Corresponds to variant rs33927108 [ dbSNP | Ensembl ].

VAR_055830

Natural variant

137

1

R → C.
Corresponds to variant rs3802989 [ dbSNP | Ensembl ].

VAR_055831

Natural variant

370

1

S → A. Ref.2
Corresponds to variant rs11544238 [ dbSNP | Ensembl ].

VAR_055832

Experimental info

Sequence conflict

152

1

L → F in AAH06107. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

750

Helix Strand Turn

Details...

Beta strand

322 – 336

15

Beta strand

346 – 361

16

Beta strand

380 – 384

5

Beta strand

389 – 392

4

Beta strand

398 – 406

9

Beta strand

412 – 416

5

Helix

420 – 439

20

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 7E769DBC3678DB49	FASTA	750	83,260
	10 20 30 40 50 60 MLSSRWWPSS WGILGLGPRS PPRGSQLCAL YAFTYTGADG QQVSLAEGDR FLLLRKTNSD 70 80 90 100 110 120 WWLARRLEAP STSRPIFVPA AYMIEESIPS QSPTTVIPGQ LLWTPGPKLF HGSLEELSQA 130 140 150 160 170 180 LPSRAQASSE QPPPLPRKMC RSVSTDNLSP SLLKPFQEGP SGRSLSQEDL PSEASASTAG 190 200 210 220 230 240 PQPLMSEPPV YCNLVDLRRC PRSPPPGPAC PLLQRLDAWE QHLDPNSGRC FYINSLTGCK 250 260 270 280 290 300 SWKPPRRSRS ETNPGSMEGT QTLKRNNDVL QPQAKGFRSD TGTPEPLDPQ GSLSLSQRTS 310 320 330 340 350 360 QLDPPALQAP RPLPQLLDDP HEVEKSGLLN MTKIAQGGRK LRKNWGPSWV VLTGNSLVFY 370 380 390 400 410 420 REPPPTAPSS GWGPAGSRPE SSVDLRGAAL AHGRHLSSRR NVLHIRTIPG HEFLLQSDHE 430 440 450 460 470 480 TELRAWHRAL RTVIERLVRW VEARREAPTG RDQGSGDREN PLELRLSGSG PAELSAGEDE 490 500 510 520 530 540 EEESELVSKP LLRLSSRRSS IRGPEGTEQN RVRNKLKRLI AKRPPLQSLQ ERGLLRDQVF 550 560 570 580 590 600 GCQLESLCQR EGDTVPSFLR LCIAAVDKRG LDVDGIYRVS GNLAVVQKLR FLVDRERAVT 610 620 630 640 650 660 SDGRYVFPEQ PGQEGRLDLD STEWDDIHVV TGALKLFLRE LPQPLVPPLL LPHFRAALAL 670 680 690 700 710 720 SESEQCLSQI QELIGSMPKP NHDTLRYLLE HLCRVIAHSD KNRMTPHNLG IVFGPTLFRP 730 740 750 EQETSDPAAH ALYPGQLVQL MLTNFTSLFP
	Isoform 2 [UniParc]. Checksum: B63901EACA109D02	FASTA	731	81,150
	10 20 30 40 50 60 MLSSRWWPSS WGILGLGPRS PPRGSQLCAL YAFTYTGADG QQVSLAEGDR FLLLRKTNSD 70 80 90 100 110 120 WWLARRLEAP STSRPIFVPA AYMIEESIPS QSPTTVIPGQ LLWTPGPKLF HGSLEELSQA 130 140 150 160 170 180 LPSRAQASSE QPPPLPRKMC RSVSTDNLSP SLLKPFQEGP SGRSLSQEDL PSEASASTAG 190 200 210 220 230 240 PQPLMSEPPV YCNLVDLRRC PRSPPPGPAC PLLQRLDAWE QHLDPNSGRC FYINSLTGCK 250 260 270 280 290 300 SWKPPRRSRS ETNPGSMEGT QTLKRNNDVL QPQAKGFRSD TGTPEPLDPQ GSLSLSQRTS 310 320 330 340 350 360 QLDPPALQAP RPLPQLLDDP HEVEKSGLLN MTKIAQGGRK LRKNWGPSWV VLTGNSLVFY 370 380 390 400 410 420 REPPPTAPSS GWGPAGSRPE SSVDLRGAAL AHGRHLSSRR NVLHIRTIPG HEFLLQSDHE 430 440 450 460 470 480 TELRAWHRAL RTVIERLDRE NPLELRLSGS GPAELSAGED EEEESELVSK PLLRLSSRRS 490 500 510 520 530 540 SIRGPEGTEQ NRVRNKLKRL IAKRPPLQSL QERGLLRDQV FGCQLESLCQ REGDTVPSFL 550 560 570 580 590 600 RLCIAAVDKR GLDVDGIYRV SGNLAVVQKL RFLVDRERAV TSDGRYVFPE QPGQEGRLDL 610 620 630 640 650 660 DSTEWDDIHV VTGALKLFLR ELPQPLVPPL LLPHFRAALA LSESEQCLSQ IQELIGSMPK 670 680 690 700 710 720 PNHDTLRYLL EHLCRVIAHS DKNRMTPHNL GIVFGPTLFR PEQETSDPAA HALYPGQLVQ 730 LMLTNFTSLF P
	Isoform 3 [UniParc]. Checksum: 68FC77CAF2B9321E	FASTA	566	63,292
	10 20 30 40 50 60 MSEPPVYCNL VDLRRCPRSP PPGPACPLLQ RLDAWEQHLD PNSGRCFYIN SLTGCKSWKP 70 80 90 100 110 120 PRRSRSETNP GSMEGTQTLK RNNDVLQPQA KGFRSDTGTP EPLDPQGSLS LSQRTSQLDP 130 140 150 160 170 180 PALQAPRPLP QLLDDPHEVE KSGLLNMTKI AQGGRKLRKN WGPSWVVLTG NSLVFYREPP 190 200 210 220 230 240 PTAPSSGWGP AGSRPESSVD LRGAALAHGR HLSSRRNVLH IRTIPGHEFL LQSDHETELR 250 260 270 280 290 300 AWHRALRTVI ERLVRWVEAR REAPTGRDQG SGDRENPLEL RLSGSGPAEL SAGEDEEEES 310 320 330 340 350 360 ELVSKPLLRL SSRRSSIRGP EGTEQNRVRN KLKRLIAKRP PLQSLQERGL LRDQVFGCQL 370 380 390 400 410 420 ESLCQREGDT VPSFLRLCIA AVDKRGLDVD GIYRVSGNLA VVQKLRFLVD RERAVTSDGR 430 440 450 460 470 480 YVFPEQPGQE GRLDLDSTEW DDIHVVTGAL KLFLRELPQP LVPPLLLPHF RAALALSESE 490 500 510 520 530 540 QCLSQIQELI GSMPKPNHDT LRYLLEHLCR VIAHSDKNRM TPHNLGIVFG PTLFRPEQET 550 560 SDPAAHALYP GQLVQLMLTN FTSLFP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a novel human gene, ARHGAP9, encoding a rho-GTPase activating protein." Furukawa Y., Kawasoe T., Daigo Y., Nishiwaki T., Ishiguro H., Takahashi M., Kitayama J., Nakamura Y. Biochem. Biophys. Res. Commun. 284:643-649(2001) [PubMed: 11396949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ALA-370. Tissue: Lymph and Muscle.
[3]	"Isolation, mapping, and characterization of a novel member of human rho-GAP family." Kawasoe T., Furukawa Y., Daigo Y., Nishiwaki T., Ishiguro H., Fujita M., Ogawa M., Nakamura Y. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 384-750 (ISOFORM 2). Tissue: Lymph node.
[5]	"Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment." Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J. J. Proteome Res. 7:5167-5176(2008) [PubMed: 19367720] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-475, MASS SPECTROMETRY. Tissue: T-cell.
[6]	"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." Voss M., Lettau M., Janssen O. BMC Immunol. 10:53-53(2009) [PubMed: 19807924] [Abstract] Cited for: INTERACTION WITH FASLG.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-475, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB051853 mRNA. Translation: BAB56159.1.
BC006107 mRNA. Translation: AAH06107.1.
BC011820 mRNA. Translation: AAH11820.1.
AB030239 mRNA. Translation: BAB83128.1.
AL713803 mRNA. Translation: CAD28552.1.

IPI

IPI00414939.
IPI00442053.
IPI00793722.

PIR

JC7701.

RefSeq

NP_001073625.1. NM_001080156.1.
NP_001073626.1. NM_001080157.1.
NP_115885.2. NM_032496.2.

UniGene

Hs.437126.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P0D	X-ray	1.81	A	321-440	[»]
2P0F	X-ray	1.91	A	321-440	[»]
2P0H	X-ray	1.90	A	321-440	[»]

ProteinModelPortal

Q9BRR9.

SMR

Q9BRR9. Positions 38-88, 321-438, 523-749.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9BRR9. 7 interactions.

MINT

MINT-1190172.

STRING

Q9BRR9.

PTM databases

PhosphoSite

Q9BRR9.

Polymorphism databases

DMDM

47117294.

Proteomic databases

PRIDE

Q9BRR9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000356411; ENSP00000348782; ENSG00000123329.
ENST00000393797; ENSP00000377386; ENSG00000123329.

GeneID

64333.

KEGG

hsa:64333.

UCSC

uc001snz.1. human.
uc001soc.1. human.

Organism-specific databases

CTD

64333.

GeneCards

GC12M057866.

H-InvDB

HIX0010768.

HGNC

HGNC:14130. ARHGAP9.

MIM

610576. gene.

neXtProt

NX_Q9BRR9.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00600000084329.

HOGENOM

HBG505851.

HOVERGEN

HBG005328.

InParanoid

Q9BRR9.

PhylomeDB

Q9BRR9.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

Q9BRR9.

Bgee

Q9BRR9.

CleanEx

HS_ARHGAP9.

Genevestigator

Q9BRR9.

GermOnline

ENSG00000123329. Homo sapiens.

Family and domain databases

InterPro

IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR015767. Rho_GTPase_activating.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_Rsp5_WWP.
[Graphical view]

Gene3D

G3DSA:2.20.70.10. G3DSA:2.20.70.10. 1 hit.
G3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:1.10.555.10. RhoGAP. 1 hit.

PANTHER

PTHR23181. Rho_GTPase_activating. 1 hit.

Pfam

PF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF48350. Rho_GAP. 1 hit.
SSF50044. SH3. 1 hit.

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS01159. WW_DOMAIN_1. False negative.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

66267.

SOURCE

Search...

Entry information

Entry name

RHG09_HUMAN

Accession

Primary (citable) accession number: Q9BRR9
Secondary accession number(s): Q8TCJ3 , Q8WYR0, Q96EZ2, Q96S74

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	January 25, 2012
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.