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Protein

Rho GTPase-activating protein 9

Gene

ARHGAP9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. Binds phosphoinositides, and has the highest affinity for phosphatidylinositol 3,4,5-trisphosphate, followed by phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: MGI
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000123329-MONOMER.
ReactomeiR-HSA-194840. Rho GTPase cycle.
R-HSA-6798695. Neutrophil degranulation.
SignaLinkiQ9BRR9.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 9
Alternative name(s):
Rho-type GTPase-activating protein 9
Gene namesi
Name:ARHGAP9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:14130. ARHGAP9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343K → A: Strongly reduced affinity for phosphoinositides. 1 Publication1
Mutagenesisi399R → A: Reduced affinity for phosphoinositides. 1 Publication1

Organism-specific databases

DisGeNETi64333.
OpenTargetsiENSG00000123329.
PharmGKBiPA24962.

Polymorphism and mutation databases

BioMutaiARHGAP9.
DMDMi47117294.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000567121 – 750Rho GTPase-activating protein 9Add BLAST750

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei475PhosphoserineCombined sources1
Modified residuei500PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9BRR9.
PeptideAtlasiQ9BRR9.
PRIDEiQ9BRR9.

PTM databases

iPTMnetiQ9BRR9.
PhosphoSitePlusiQ9BRR9.

Expressioni

Tissue specificityi

Predominantly expressed in peripheral blood leukocytes, spleen, and thymus.1 Publication

Gene expression databases

BgeeiENSG00000123329.
CleanExiHS_ARHGAP9.
ExpressionAtlasiQ9BRR9. baseline and differential.
GenevisibleiQ9BRR9. HS.

Organism-specific databases

HPAiHPA051447.

Interactioni

Subunit structurei

Interacts with FASLG.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
FHL2Q141923EBI-750254,EBI-701903
GRB2P629933EBI-750254,EBI-401755

Protein-protein interaction databases

BioGridi122140. 9 interactors.
IntActiQ9BRR9. 10 interactors.
MINTiMINT-1190172.
STRINGi9606.ENSP00000377380.

Structurei

Secondary structure

1750
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi322 – 336Combined sources15
Beta strandi346 – 361Combined sources16
Helixi368 – 370Combined sources3
Beta strandi380 – 384Combined sources5
Beta strandi389 – 392Combined sources4
Turni394 – 396Combined sources3
Beta strandi398 – 406Combined sources9
Beta strandi412 – 416Combined sources5
Helixi420 – 439Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P0DX-ray1.81A321-440[»]
2P0FX-ray1.91A321-440[»]
2P0HX-ray1.90A321-440[»]
ProteinModelPortaliQ9BRR9.
SMRiQ9BRR9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BRR9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 88SH3PROSITE-ProRule annotationAdd BLAST67
Domaini213 – 247WWPROSITE-ProRule annotationAdd BLAST35
Domaini322 – 435PHPROSITE-ProRule annotationAdd BLAST114
Domaini542 – 749Rho-GAPPROSITE-ProRule annotationAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni342 – 345Lipid binding4
Regioni397 – 399Lipid binding3
Regioni432 – 669Lipid bindingAdd BLAST238

Domaini

A region including the PH domain and partially overlapping with the Rho-GAP domain mediates interaction with phosphoinositides.1 Publication

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 WW domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG1450. Eukaryota.
ENOG410ZP6T. LUCA.
GeneTreeiENSGT00840000129684.
HOGENOMiHOG000294167.
HOVERGENiHBG005328.
InParanoidiQ9BRR9.
KOiK20634.
PhylomeDBiQ9BRR9.
TreeFamiTF329345.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 1 hit.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51045. SSF51045. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BRR9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSSRWWPSS WGILGLGPRS PPRGSQLCAL YAFTYTGADG QQVSLAEGDR
60 70 80 90 100
FLLLRKTNSD WWLARRLEAP STSRPIFVPA AYMIEESIPS QSPTTVIPGQ
110 120 130 140 150
LLWTPGPKLF HGSLEELSQA LPSRAQASSE QPPPLPRKMC RSVSTDNLSP
160 170 180 190 200
SLLKPFQEGP SGRSLSQEDL PSEASASTAG PQPLMSEPPV YCNLVDLRRC
210 220 230 240 250
PRSPPPGPAC PLLQRLDAWE QHLDPNSGRC FYINSLTGCK SWKPPRRSRS
260 270 280 290 300
ETNPGSMEGT QTLKRNNDVL QPQAKGFRSD TGTPEPLDPQ GSLSLSQRTS
310 320 330 340 350
QLDPPALQAP RPLPQLLDDP HEVEKSGLLN MTKIAQGGRK LRKNWGPSWV
360 370 380 390 400
VLTGNSLVFY REPPPTAPSS GWGPAGSRPE SSVDLRGAAL AHGRHLSSRR
410 420 430 440 450
NVLHIRTIPG HEFLLQSDHE TELRAWHRAL RTVIERLVRW VEARREAPTG
460 470 480 490 500
RDQGSGDREN PLELRLSGSG PAELSAGEDE EEESELVSKP LLRLSSRRSS
510 520 530 540 550
IRGPEGTEQN RVRNKLKRLI AKRPPLQSLQ ERGLLRDQVF GCQLESLCQR
560 570 580 590 600
EGDTVPSFLR LCIAAVDKRG LDVDGIYRVS GNLAVVQKLR FLVDRERAVT
610 620 630 640 650
SDGRYVFPEQ PGQEGRLDLD STEWDDIHVV TGALKLFLRE LPQPLVPPLL
660 670 680 690 700
LPHFRAALAL SESEQCLSQI QELIGSMPKP NHDTLRYLLE HLCRVIAHSD
710 720 730 740 750
KNRMTPHNLG IVFGPTLFRP EQETSDPAAH ALYPGQLVQL MLTNFTSLFP
Length:750
Mass (Da):83,260
Last modified:May 10, 2004 - v2
Checksum:i7E769DBC3678DB49
GO
Isoform 2 (identifier: Q9BRR9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     438-456: Missing.

Show »
Length:731
Mass (Da):81,150
Checksum:iB63901EACA109D02
GO
Isoform 3 (identifier: Q9BRR9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-184: Missing.

Show »
Length:566
Mass (Da):63,292
Checksum:i68FC77CAF2B9321E
GO
Isoform 4 (identifier: Q9BRR9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-184: Missing.
     438-456: Missing.

Show »
Length:547
Mass (Da):61,183
Checksum:iBA337CC08F1B433B
GO
Isoform 5 (identifier: Q9BRR9-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     438-456: Missing.
     659-750: ALSESEQCLS...MLTNFTSLFP → G

Note: No experimental confirmation available.
Show »
Length:640
Mass (Da):70,869
Checksum:i163BA91817EE36F2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152L → F in AAH06107 (Ref. 2) Curated1
Sequence conflicti195V → A in BAC03969 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05583050R → G.Corresponds to variant rs33927108dbSNPEnsembl.1
Natural variantiVAR_055831137R → C.Corresponds to variant rs3802989dbSNPEnsembl.1
Natural variantiVAR_055832370S → A.2 PublicationsCorresponds to variant rs11544238dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0103401 – 184Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST184
Alternative sequenceiVSP_010325438 – 456Missing in isoform 2, isoform 4 and isoform 5. 4 PublicationsAdd BLAST19
Alternative sequenceiVSP_046391659 – 750ALSES…TSLFP → G in isoform 5. 1 PublicationAdd BLAST92

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051853 mRNA. Translation: BAB56159.1.
AB030239 mRNA. Translation: BAB83128.1.
AK092763 mRNA. Translation: BAC03969.1.
AK301095 mRNA. Translation: BAG62695.1.
AC022506 Genomic DNA. No translation available.
BC006107 mRNA. Translation: AAH06107.1.
BC011820 mRNA. Translation: AAH11820.1.
AL713803 mRNA. Translation: CAD28552.1.
CCDSiCCDS44928.1. [Q9BRR9-4]
CCDS44929.1. [Q9BRR9-5]
CCDS8941.2. [Q9BRR9-2]
PIRiJC7701.
RefSeqiNP_001073625.1. NM_001080156.2. [Q9BRR9-4]
NP_001073626.1. NM_001080157.1. [Q9BRR9-5]
NP_001306779.1. NM_001319850.1.
NP_001306780.1. NM_001319851.1.
NP_001306781.1. NM_001319852.1. [Q9BRR9-3]
NP_115885.2. NM_032496.3. [Q9BRR9-2]
XP_005269140.1. XM_005269083.2. [Q9BRR9-3]
XP_011536958.1. XM_011538656.2. [Q9BRR9-2]
XP_011536961.1. XM_011538659.2. [Q9BRR9-5]
XP_016875289.1. XM_017019800.1. [Q9BRR9-3]
XP_016875290.1. XM_017019801.1. [Q9BRR9-4]
UniGeneiHs.437126.

Genome annotation databases

EnsembliENST00000393791; ENSP00000377380; ENSG00000123329. [Q9BRR9-2]
ENST00000424809; ENSP00000394307; ENSG00000123329. [Q9BRR9-5]
ENST00000430041; ENSP00000397950; ENSG00000123329. [Q9BRR9-4]
GeneIDi64333.
KEGGihsa:64333.
UCSCiuc001snz.4. human. [Q9BRR9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051853 mRNA. Translation: BAB56159.1.
AB030239 mRNA. Translation: BAB83128.1.
AK092763 mRNA. Translation: BAC03969.1.
AK301095 mRNA. Translation: BAG62695.1.
AC022506 Genomic DNA. No translation available.
BC006107 mRNA. Translation: AAH06107.1.
BC011820 mRNA. Translation: AAH11820.1.
AL713803 mRNA. Translation: CAD28552.1.
CCDSiCCDS44928.1. [Q9BRR9-4]
CCDS44929.1. [Q9BRR9-5]
CCDS8941.2. [Q9BRR9-2]
PIRiJC7701.
RefSeqiNP_001073625.1. NM_001080156.2. [Q9BRR9-4]
NP_001073626.1. NM_001080157.1. [Q9BRR9-5]
NP_001306779.1. NM_001319850.1.
NP_001306780.1. NM_001319851.1.
NP_001306781.1. NM_001319852.1. [Q9BRR9-3]
NP_115885.2. NM_032496.3. [Q9BRR9-2]
XP_005269140.1. XM_005269083.2. [Q9BRR9-3]
XP_011536958.1. XM_011538656.2. [Q9BRR9-2]
XP_011536961.1. XM_011538659.2. [Q9BRR9-5]
XP_016875289.1. XM_017019800.1. [Q9BRR9-3]
XP_016875290.1. XM_017019801.1. [Q9BRR9-4]
UniGeneiHs.437126.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P0DX-ray1.81A321-440[»]
2P0FX-ray1.91A321-440[»]
2P0HX-ray1.90A321-440[»]
ProteinModelPortaliQ9BRR9.
SMRiQ9BRR9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122140. 9 interactors.
IntActiQ9BRR9. 10 interactors.
MINTiMINT-1190172.
STRINGi9606.ENSP00000377380.

PTM databases

iPTMnetiQ9BRR9.
PhosphoSitePlusiQ9BRR9.

Polymorphism and mutation databases

BioMutaiARHGAP9.
DMDMi47117294.

Proteomic databases

PaxDbiQ9BRR9.
PeptideAtlasiQ9BRR9.
PRIDEiQ9BRR9.

Protocols and materials databases

DNASUi64333.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393791; ENSP00000377380; ENSG00000123329. [Q9BRR9-2]
ENST00000424809; ENSP00000394307; ENSG00000123329. [Q9BRR9-5]
ENST00000430041; ENSP00000397950; ENSG00000123329. [Q9BRR9-4]
GeneIDi64333.
KEGGihsa:64333.
UCSCiuc001snz.4. human. [Q9BRR9-1]

Organism-specific databases

CTDi64333.
DisGeNETi64333.
GeneCardsiARHGAP9.
H-InvDBHIX0010768.
HGNCiHGNC:14130. ARHGAP9.
HPAiHPA051447.
MIMi610576. gene.
neXtProtiNX_Q9BRR9.
OpenTargetsiENSG00000123329.
PharmGKBiPA24962.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1450. Eukaryota.
ENOG410ZP6T. LUCA.
GeneTreeiENSGT00840000129684.
HOGENOMiHOG000294167.
HOVERGENiHBG005328.
InParanoidiQ9BRR9.
KOiK20634.
PhylomeDBiQ9BRR9.
TreeFamiTF329345.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000123329-MONOMER.
ReactomeiR-HSA-194840. Rho GTPase cycle.
R-HSA-6798695. Neutrophil degranulation.
SignaLinkiQ9BRR9.

Miscellaneous databases

ChiTaRSiARHGAP9. human.
EvolutionaryTraceiQ9BRR9.
GeneWikiiARHGAP9.
GenomeRNAii64333.
PROiQ9BRR9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123329.
CleanExiHS_ARHGAP9.
ExpressionAtlasiQ9BRR9. baseline and differential.
GenevisibleiQ9BRR9. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR015767. Rho_GTPase_act.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERiPTHR23181. PTHR23181. 1 hit.
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51045. SSF51045. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS50020. WW_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG09_HUMAN
AccessioniPrimary (citable) accession number: Q9BRR9
Secondary accession number(s): B4DVI3
, E9PDX9, Q8NAF3, Q8TCJ3, Q8WYR0, Q96EZ2, Q96S74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.