Q9BRR6 (ADPGK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP-dependent glucokinase
Short name=ADP-GK
Short name=ADPGK
EC=2.7.1.147

Alternative name(s):
RbBP-35

Gene names

Name:	ADPGK
ORF Names:	PSEC0260

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency By similarity.
Catalytic activity	ADP + D-glucose = AMP + D-glucose 6-phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Carbohydrate degradation; glycolysis.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the ADP-dependent glucokinase family. Contains 1 ADPK (ADP-dependent kinase) domain.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ADP-specific glucokinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BRR6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BRR6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     313-313: Missing.

Isoform 3 (identifier: Q9BRR6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-239: Missing.

Isoform 4 (identifier: Q9BRR6-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-274: Missing.
     275-280: RKRLLE → MAASCR
     313-313: Missing.

Isoform 5 (identifier: Q9BRR6-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.
     404-469: AAGARVAGTQ...ISFHFTPVLV → LGSSCGWDTG...ILPLHTSIGV
     470-497: Missing.

Isoform 6 (identifier: Q9BRR6-6)

The sequence of this isoform differs from the canonical sequence as follows:
     79-497: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 497	475	ADP-dependent glucokinase		PRO_0000184776
Regions
	Domain	52 – 497	446	ADPK
Sites
	Active site	481	1	Proton acceptor By similarity
	Metal binding	297	1	Magnesium By similarity
	Metal binding	328	1	Magnesium By similarity
	Metal binding	481	1	Magnesium By similarity
Natural variations
	Alternative sequence	1 – 274	274	Missing in isoform 4.		VSP_013550
	Alternative sequence	1 – 239	239	Missing in isoform 3.		VSP_013549
	Alternative sequence	1 – 122	122	Missing in isoform 5.		VSP_013548
	Alternative sequence	79 – 497	419	Missing in isoform 6.		VSP_035014
	Alternative sequence	275 – 280	6	RKRLLE → MAASCR in isoform 4.		VSP_013551
	Alternative sequence	313	1	Missing in isoform 2 and isoform 4.		VSP_013552
	Alternative sequence	404 – 469	66	AAGAR…TPVLV → LGSSCGWDTGLRHRNHRHQP SVSEGTPRVHDFPFGGRLQD CIKPKQASSRMAQRGNILPL HTSIGV in isoform 5.		VSP_013553
	Alternative sequence	470 – 497	28	Missing in isoform 5.		VSP_013554
	Natural variant	184	1	K → R. Corresponds to variant rs8024644 [ dbSNP | Ensembl ].		VAR_060085

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B758E977CDA88F8F	FASTA	497	54,089
	10 20 30 40 50 60 MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA 70 80 90 100 110 120 WDALIVRPVR RWRRVAVGVN ACVDVVLSGV KLLQALGLSP GNGKDHSILH SRNDLEEAFI 130 140 150 160 170 180 HFMGKGAAAE RFFSDKETFH DIAQVASEFP GAQHYVGGNA ALIGQKFAAN SDLKVLLCGP 190 200 210 220 230 240 VGPKLHELLD DNVFVPPESL QEVDEFHLIL EYQAGEEWGQ LKAPHANRFI FSHDLSNGAM 250 260 270 280 290 300 NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTSISDIPT GIPVHLELAS 310 320 330 340 350 360 MTNRELMSSI VHQQVFPAVT SLGLNEQELL FLTQSASGPH SSLSSWNGVP DVGMVSDILF 370 380 390 400 410 420 WILKEHGRSK SRASDLTRIH FHTLVYHILA TVDGHWANQL AAVAAGARVA GTQACATETI 430 440 450 460 470 480 DTSRVSLRAP QEFMTSHSEA GSRIVLNPNK PVVEWHREGI SFHFTPVLVC KDPIRTVGLG 490 DAISAEGLFY SEVHPHY
	Isoform 2 [UniParc]. Checksum: 5ACBE523E6EE8828	FASTA	496	53,961
	10 20 30 40 50 60 MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA 70 80 90 100 110 120 WDALIVRPVR RWRRVAVGVN ACVDVVLSGV KLLQALGLSP GNGKDHSILH SRNDLEEAFI 130 140 150 160 170 180 HFMGKGAAAE RFFSDKETFH DIAQVASEFP GAQHYVGGNA ALIGQKFAAN SDLKVLLCGP 190 200 210 220 230 240 VGPKLHELLD DNVFVPPESL QEVDEFHLIL EYQAGEEWGQ LKAPHANRFI FSHDLSNGAM 250 260 270 280 290 300 NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTSISDIPT GIPVHLELAS 310 320 330 340 350 360 MTNRELMSSI VHQVFPAVTS LGLNEQELLF LTQSASGPHS SLSSWNGVPD VGMVSDILFW 370 380 390 400 410 420 ILKEHGRSKS RASDLTRIHF HTLVYHILAT VDGHWANQLA AVAAGARVAG TQACATETID 430 440 450 460 470 480 TSRVSLRAPQ EFMTSHSEAG SRIVLNPNKP VVEWHREGIS FHFTPVLVCK DPIRTVGLGD 490 AISAEGLFYS EVHPHY
	Isoform 3 [UniParc]. Checksum: 14A1E87AB18DDB1B	FASTA	258	28,413
	10 20 30 40 50 60 MNMLEVFVSS LEEFQPDLVV LSGLHMMEGQ SKELQRKRLL EVVTSISDIP TGIPVHLELA 70 80 90 100 110 120 SMTNRELMSS IVHQQVFPAV TSLGLNEQEL LFLTQSASGP HSSLSSWNGV PDVGMVSDIL 130 140 150 160 170 180 FWILKEHGRS KSRASDLTRI HFHTLVYHIL ATVDGHWANQ LAAVAAGARV AGTQACATET 190 200 210 220 230 240 IDTSRVSLRA PQEFMTSHSE AGSRIVLNPN KPVVEWHREG ISFHFTPVLV CKDPIRTVGL 250 GDAISAEGLF YSEVHPHY
	Isoform 4 [UniParc]. Checksum: F387E8FABA303003	FASTA	222	24,130
	10 20 30 40 50 60 MAASCRVVTS ISDIPTGIPV HLELASMTNR ELMSSIVHQV FPAVTSLGLN EQELLFLTQS 70 80 90 100 110 120 ASGPHSSLSS WNGVPDVGMV SDILFWILKE HGRSKSRASD LTRIHFHTLV YHILATVDGH 130 140 150 160 170 180 WANQLAAVAA GARVAGTQAC ATETIDTSRV SLRAPQEFMT SHSEAGSRIV LNPNKPVVEW 190 200 210 220 HREGISFHFT PVLVCKDPIR TVGLGDAISA EGLFYSEVHP HY
	Isoform 5 [UniParc]. Checksum: 6D5F512F495A60F6	FASTA	347	38,254
	10 20 30 40 50 60 MGKGAAAERF FSDKETFHDI AQVASEFPGA QHYVGGNAAL IGQKFAANSD LKVLLCGPVG 70 80 90 100 110 120 PKLHELLDDN VFVPPESLQE VDEFHLILEY QAGEEWGQLK APHANRFIFS HDLSNGAMNM 130 140 150 160 170 180 LEVFVSSLEE FQPDLVVLSG LHMMEGQSKE LQRKRLLEVV TSISDIPTGI PVHLELASMT 190 200 210 220 230 240 NRELMSSIVH QQVFPAVTSL GLNEQELLFL TQSASGPHSS LSSWNGVPDV GMVSDILFWI 250 260 270 280 290 300 LKEHGRSKSR ASDLTRIHFH TLVYHILATV DGHWANQLAA VLGSSCGWDT GLRHRNHRHQ 310 320 330 340 PSVSEGTPRV HDFPFGGRLQ DCIKPKQASS RMAQRGNILP LHTSIGV
	Isoform 6 [UniParc]. Checksum: CE817CAB68D45EB9	FASTA	78	8,252
	10 20 30 40 50 60 MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA 70 WDALIVRPVR RWRRVAVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RbBP-35 interacted with pRb." Fan Z.S., Ao S.Z. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5). Tissue: Lymph node.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Heart.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
[5]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Teratocarcinoma.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 6). Tissue: Brain and Kidney.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF204270 mRNA. Translation: AAL31473.1. AL136873 mRNA. Translation: CAB66807.1. AK055526 mRNA. Translation: BAB70941.1. CR533447 mRNA. Translation: CAG38478.1. AK075560 mRNA. Translation: BAC11699.1. BC006112 mRNA. Translation: AAH06112.1. BC018074 mRNA. Translation: AAH18074.1.
IPI	IPI00103892. IPI00329593. IPI00550060. IPI00556385. IPI00903303. IPI00940629.
RefSeq	NP_112574.3. NM_031284.4.
UniGene	Hs.654636.
3D structure databases
ProteinModelPortal	Q9BRR6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BRR6. 5 interactions.
MINT	MINT-1404876.
STRING	9606.ENSP00000312250.
PTM databases
PhosphoSite	Q9BRR6.
Polymorphism databases
DMDM	62899887.
Proteomic databases
PaxDb	Q9BRR6.
PRIDE	Q9BRR6.
Protocols and materials databases
DNASU	83440.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000311669; ENSP00000312250; ENSG00000159322. ENST00000456471; ENSP00000397694; ENSG00000159322. ENST00000562823; ENSP00000454367; ENSG00000159322. ENST00000567941; ENSP00000458102; ENSG00000159322. ENST00000569517; ENSP00000454304; ENSG00000159322.
GeneID	83440.
KEGG	hsa:83440.
UCSC	uc002ave.4. human. uc002avf.4. human. uc002avg.4. human.
Organism-specific databases
CTD	83440.
GeneCards	GC15M073043.
H-InvDB	HIX0026868.
HGNC	HGNC:25250. ADPGK.
HPA	HPA045194.
MIM	611861. gene.
neXtProt	NX_Q9BRR6.
PharmGKB	PA134971928.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG4809.
HOGENOM	HOG000233731.
HOVERGEN	HBG050461.
InParanoid	Q9BRR6.
KO	K08074.
OMA	MMEGQSK.
OrthoDB	EOG47WNNN.
Enzyme and pathway databases
UniPathway	UPA00109.
Gene expression databases
ArrayExpress	Q9BRR6.
Bgee	Q9BRR6.
CleanEx	HS_ADPGK.
Genevestigator	Q9BRR6.
GermOnline	ENSG00000159322. Homo sapiens.
Family and domain databases
InterPro	IPR007666. ADP_PFK/GK. [Graphical view]
PANTHER	PTHR21208. PTHR21208. 1 hit.
Pfam	PF04587. ADP_PFK_GK. 1 hit. [Graphical view]
PROSITE	PS51255. ADPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	83440.
NextBio	72318.
SOURCE	Search...

Entry information

Entry name

ADPGK_HUMAN

Accession

Primary (citable) accession number: Q9BRR6
Secondary accession number(s): Q49AU7 , Q8NBI1, Q8WZ90, Q96NF8, Q9H0A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.