Reviewed,
UniProtKB/Swiss-Prot Q9BRQ0 (PYGO2_HUMAN)
Last modified
January 19, 2010.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Pygopus homolog 2 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 406 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in signal transduction through the Wnt pathway. |
| Subunit structure | Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex. |
| Subcellular location | Nucleus Probable. |
| Sequence similarities | Contains 1 PHD-type zinc finger. |
| Sequence caution | The sequence AAL55782.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Wnt signaling pathway |
| Cellular component | Nucleus |
| Domain | Zinc-finger |
| Ligand | Metal-binding Zinc |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 406 | 405 | Pygopus homolog 2 | PRO_0000097123 | |||||
Regions | |||||||||
| Zinc finger | 327 – 385 | 59 | PHD-type | ||||||
| Motif | 41 – 47 | 7 | Nuclear localization signal Potential | ||||||
| Compositional bias | 6 – 329 | 324 | Pro-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.8 | ||||||
| Modified residue | 97 | 1 | Phosphoserine Ref.5 Ref.7 | ||||||
| Modified residue | 302 | 1 | Phosphothreonine Ref.8 Ref.7 Ref.4 Ref.6 Ref.9 | ||||||
Experimental info | |||||||||
| Sequence conflict | 157 | 1 | P → Q in AAL55782. Ref.3 | ||||||
| Sequence conflict | 170 | 1 | P → L in AAL55782. Ref.3 | ||||||
Sequences
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References
| [1] | "Wnt/wingless signaling requires BCL9/legless-mediated recruitment of pygopus to the nuclear beta-catenin-TCF complex." Kramps T., Peter O., Brunner E., Nellen D., Froesch B., Chatterjee S., Murone M., Zuellig S., Basler K. Cell 109:47-60(2002) [PubMed: 11955446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Ovary. |
| [3] | "Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. Gu J.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-406. |
| [4] | "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. Tissue: Epithelium. |
| [5] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. |
| [7] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97 AND THR-302, MASS SPECTROMETRY. |
| [8] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. |
| [9] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF457208 mRNA. Translation: AAL91371.1. BC006132 mRNA. Translation: AAH06132.2. BC013725 mRNA. Translation: AAH13725.1. BC032099 mRNA. Translation: AAH32099.1. AF289598 mRNA. Translation: AAL55782.1. Sequence problems. |
| IPI | IPI00042099. |
| RefSeq | NP_612157.1. |
| UniGene | Hs.533597 |
3D structure databases | |
| SMR | Q9BRQ0. Positions 327-383. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9BRQ0. 2 interactions. |
| STRING | Q9BRQ0. |
PTM databases | |
| PhosphoSite | Q9BRQ0. |
Proteomic databases | |
| PRIDE | Q9BRQ0. |
Genome annotation databases | |
| Ensembl | ENST00000368457; ENSP00000357442; ENSG00000163348; Homo sapiens. [Genome view] |
| GeneID | 90780. |
| KEGG | hsa:90780. |
| UCSC | uc001fft.1. human. |
Organism-specific databases | |
| CTD | 90780. |
| GeneCards | GC01M153196. |
| H-InvDB | HIX0001024. |
| HGNC | HGNC:30257. PYGO2. |
| HPA | HPA023689. |
| MIM | 606903. gene. |
| PharmGKB | PA134881185. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HBG714877. |
| HOVERGEN | Q9BRQ0. |
| InParanoid | Q9BRQ0. |
| OMA | SPPSGQM. |
| OrthoDB | EOG9PZMSP. |
| PhylomeDB | Q9BRQ0. |
Gene expression databases | |
| ArrayExpress | Q9BRQ0. |
| Bgee | Q9BRQ0. |
| CleanEx | HS_PYGO2. |
| Genevestigator | Q9BRQ0. |
| GermOnline | ENSG00000163348. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. [Graphical view] |
| Pfam | PF00628. PHD. 1 hit. [Graphical view] |
| SMART | SM00249. PHD. 1 hit. [Graphical view] |
| PROSITE | PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 76956. |
| SOURCE | Search... |
Entry information
| Entry name | PYGO2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9BRQ0 Secondary accession number(s): Q8WYZ4, Q96CY2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


