Reviewed, UniProtKB/Swiss-Prot Q9BRQ0 (PYGO2_HUMAN)

Names and origin

Protein names

Recommended name:
    Pygopus homolog 2

Gene names

Name:	PYGO2
ORF Names:	PP7910

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in signal transduction through the Wnt pathway.
Subunit structure	Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex.
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 PHD-type zinc finger.
Sequence caution	The sequence AAL55782.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL9	O00512	1	EBI-932471,EBI-533127

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 406	405	Pygopus homolog 2		PRO_0000097123
Regions
	Zinc finger	327 – 385	59	PHD-type
	Motif	41 – 47	7	Nuclear localization signal Potential
	Compositional bias	6 – 329	324	Pro-rich
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.8
	Modified residue	97	1	Phosphoserine Ref.5 Ref.7
	Modified residue	302	1	Phosphothreonine Ref.8 Ref.7 Ref.4 Ref.6 Ref.9
Experimental info
	Sequence conflict	157	1	P → Q in AAL55782. Ref.3
	Sequence conflict	170	1	P → L in AAL55782. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9BRQ0-1 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: CA002A5447767CD9	FASTA	406	41,244
	10 20 30 40 50 60 MAASAPPPPD KLEGGGGPAP PPAPPSTGRK QGKAGLQMKS PEKKRRKSNT QGPAYSHLTE 70 80 90 100 110 120 FAPPPTPMVD HLVASNPFED DFGAPKVGVA APPFLGSPVP FGGFRVQGGM AGQVPPGYST 130 140 150 160 170 180 GGGGGPQPLR RQPPPFPPNP MGPAFNMPPQ GPGYPPPGNM NFPSQPFNQP LGQNFSPPSG 190 200 210 220 230 240 QMMPGPVGGF GPMISPTMGQ PPRAELGPPS LSQRFAQPGA PFGPSPLQRP GQGLPSLPPN 250 260 270 280 290 300 TSPFPGPDPG FPGPGGEDGG KPLNPPASTA FPQEPHSGSP AAAVNGNQPS FPPNSSGRGG 310 320 330 340 350 360 GTPDANSLAP PGKAGGGSGP QPPPGLVYPC GACRSEVNDD QDAILCEASC QKWFHRECTG 370 380 390 400 MTESAYGLLT TEASAVWACD LCLKTKEIQS VYIREGMGQL VAANDG

References

[1]	"Wnt/wingless signaling requires BCL9/legless-mediated recruitment of pygopus to the nuclear beta-catenin-TCF complex." Kramps T., Peter O., Brunner E., Nellen D., Froesch B., Chatterjee S., Murone M., Zuellig S., Basler K. Cell 109:47-60(2002) [PubMed: 11955446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Ovary.
[3]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-406.
[4]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. Tissue: Epithelium.
[5]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97 AND THR-302, MASS SPECTROMETRY.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. Tissue: T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF457208 mRNA. Translation: AAL91371.1. BC006132 mRNA. Translation: AAH06132.2. BC013725 mRNA. Translation: AAH13725.1. BC032099 mRNA. Translation: AAH32099.1. AF289598 mRNA. Translation: AAL55782.1. Sequence problems.
IPI	IPI00042099.
RefSeq	NP_612157.1.
UniGene	Hs.533597
3D structure databases
SMR	Q9BRQ0. Positions 327-383.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BRQ0. 2 interactions.
STRING	Q9BRQ0.
PTM databases
PhosphoSite	Q9BRQ0.
Proteomic databases
PRIDE	Q9BRQ0.
Genome annotation databases
Ensembl	ENST00000368457; ENSP00000357442; ENSG00000163348; Homo sapiens. [Genome view]
GeneID	90780.
KEGG	hsa:90780.
UCSC	uc001fft.1. human.
Organism-specific databases
CTD	90780.
GeneCards	GC01M153196.
H-InvDB	HIX0001024.
HGNC	HGNC:30257. PYGO2.
HPA	HPA023689.
MIM	606903. gene.
PharmGKB	PA134881185.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG714877.
HOVERGEN	Q9BRQ0.
InParanoid	Q9BRQ0.
OMA	SPPSGQM.
OrthoDB	EOG9PZMSP.
PhylomeDB	Q9BRQ0.
Gene expression databases
ArrayExpress	Q9BRQ0.
Bgee	Q9BRQ0.
CleanEx	HS_PYGO2.
Genevestigator	Q9BRQ0.
GermOnline	ENSG00000163348. Homo sapiens.
Family and domain databases
InterPro	IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. [Graphical view]
Pfam	PF00628. PHD. 1 hit. [Graphical view]
SMART	SM00249. PHD. 1 hit. [Graphical view]
PROSITE	PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	76956.
SOURCE	Search...

Entry information

Entry name

PYGO2_HUMAN

Accession

Primary (citable) accession number: Q9BRQ0
Secondary accession number(s): Q8WYZ4, Q96CY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	September 19, 2002
Last modified:	January 19, 2010
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.