Q9BRQ0 (PYGO2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pygopus homolog 2

Gene names

Name:	PYGO2
ORF Names:	PP7910

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in signal transduction through the Wnt pathway.
Subunit structure	Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex.
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 PHD-type zinc finger.
Sequence caution	The sequence AAL55782.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 406

405

Pygopus homolog 2

PRO_0000097123

Regions

Zinc finger

327 – 385

59

PHD-type

Motif

41 – 47

7

Nuclear localization signal Potential

Compositional bias

6 – 329

324

Pro-rich

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.8

Modified residue

97

1

Phosphoserine Ref.5 Ref.7

Modified residue

302

1

Phosphothreonine Ref.4 Ref.6 Ref.7 Ref.8 Ref.9

Experimental info

Sequence conflict

157

1

P → Q in AAL55782. Ref.3

Sequence conflict

170

1

P → L in AAL55782. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

406

Helix Strand Turn

Details...

Beta strand

343 – 345

3

Turn

347 – 350

4

Beta strand

353 – 355

3

Helix

356 – 358

3

Turn

363 – 365

3

Turn

369 – 371

3

Beta strand

375 – 377

3

Helix

380 – 384

5

Sequences

	Sequence		Length	Mass (Da)
	Q9BRQ0 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: CA002A5447767CD9	FASTA	406	41,244
	10 20 30 40 50 60 MAASAPPPPD KLEGGGGPAP PPAPPSTGRK QGKAGLQMKS PEKKRRKSNT QGPAYSHLTE 70 80 90 100 110 120 FAPPPTPMVD HLVASNPFED DFGAPKVGVA APPFLGSPVP FGGFRVQGGM AGQVPPGYST 130 140 150 160 170 180 GGGGGPQPLR RQPPPFPPNP MGPAFNMPPQ GPGYPPPGNM NFPSQPFNQP LGQNFSPPSG 190 200 210 220 230 240 QMMPGPVGGF GPMISPTMGQ PPRAELGPPS LSQRFAQPGA PFGPSPLQRP GQGLPSLPPN 250 260 270 280 290 300 TSPFPGPDPG FPGPGGEDGG KPLNPPASTA FPQEPHSGSP AAAVNGNQPS FPPNSSGRGG 310 320 330 340 350 360 GTPDANSLAP PGKAGGGSGP QPPPGLVYPC GACRSEVNDD QDAILCEASC QKWFHRECTG 370 380 390 400 MTESAYGLLT TEASAVWACD LCLKTKEIQS VYIREGMGQL VAANDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Wnt/wingless signaling requires BCL9/legless-mediated recruitment of pygopus to the nuclear beta-catenin-TCF complex." Kramps T., Peter O., Brunner E., Nellen D., Froesch B., Chatterjee S., Murone M., Zuellig S., Basler K. Cell 109:47-60(2002) [PubMed: 11955446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Ovary.
[3]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-406.
[4]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97 AND THR-302, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-302, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF457208 mRNA. Translation: AAL91371.1.
BC006132 mRNA. Translation: AAH06132.2.
BC013725 mRNA. Translation: AAH13725.1.
BC032099 mRNA. Translation: AAH32099.1.
AF289598 mRNA. Translation: AAL55782.1. Sequence problems.

IPI

IPI00042099.

RefSeq

NP_612157.1. NM_138300.3.

UniGene

Hs.533597.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2XB1	X-ray	1.90	A/C	325-387	[»]

ProteinModelPortal

Q9BRQ0.

SMR

Q9BRQ0. Positions 325-393.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9BRQ0. 2 interactions.

STRING

Q9BRQ0.

PTM databases

PhosphoSite

Q9BRQ0.

Polymorphism databases

DMDM

23396825.

Proteomic databases

PRIDE

Q9BRQ0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000368457; ENSP00000357442; ENSG00000163348.

GeneID

90780.

KEGG

hsa:90780.

UCSC

uc001fft.1. human.

Organism-specific databases

CTD

90780.

GeneCards

GC01M154929.

H-InvDB

HIX0001024.

HGNC

HGNC:30257. PYGO2.

HPA

HPA023689.

MIM

606903. gene.

neXtProt

NX_Q9BRQ0.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00530000063948.

HOGENOM

HBG714877.

HOVERGEN

HBG053774.

InParanoid

Q9BRQ0.

OMA

SPPSGQM.

OrthoDB

EOG4QZ7MB.

PhylomeDB

Q9BRQ0.

Gene expression databases

ArrayExpress

Q9BRQ0.

Bgee

Q9BRQ0.

CleanEx

HS_PYGO2.

Genevestigator

Q9BRQ0.

GermOnline

ENSG00000163348. Homo sapiens.

Family and domain databases

InterPro

IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

Pfam

PF00628. PHD. 1 hit.
[Graphical view]

SMART

SM00249. PHD. 1 hit.
[Graphical view]

SUPFAM

SSF57903. FYVE_PHD_ZnF. 1 hit.

PROSITE

PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

76956.

SOURCE

Search...

Entry information

Entry name

PYGO2_HUMAN

Accession

Primary (citable) accession number: Q9BRQ0
Secondary accession number(s): Q8WYZ4, Q96CY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	September 19, 2002
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.