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Protein

Serine/arginine-rich splicing factor 8

Gene

SRSF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA alternative splicing.1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 8
Alternative name(s):
Pre-mRNA-splicing factor SRP46
Short name:
Splicing factor SRp46
Splicing factor, arginine/serine-rich 2B
Gene namesi
Name:SRSF8
Synonyms:SFRS2B, SRP46
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:16988. SRSF8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165543687.

Polymorphism and mutation databases

DMDMi74761217.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 282281Serine/arginine-rich splicing factor 8PRO_0000304412Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei26 – 261PhosphoserineCombined sources
Modified residuei156 – 1561PhosphoserineCombined sources
Modified residuei158 – 1581PhosphoserineCombined sources
Modified residuei171 – 1711PhosphoserineCombined sources
Modified residuei173 – 1731PhosphoserineCombined sources
Modified residuei196 – 1961PhosphoserineCombined sources
Modified residuei273 – 2731PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BRL6.
MaxQBiQ9BRL6.
PeptideAtlasiQ9BRL6.
PRIDEiQ9BRL6.

PTM databases

iPTMnetiQ9BRL6.
PhosphoSiteiQ9BRL6.

Expressioni

Tissue specificityi

Strongly expressed in pancreas, spleen and prostate. Weakly expressed in lung, liver and thymus.1 Publication

Gene expression databases

CleanExiHS_SFRS2B.
ExpressionAtlasiQ9BRL6. baseline and differential.
GenevisibleiQ9BRL6. HS.

Organism-specific databases

HPAiHPA049905.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SRPK1Q96SB42EBI-6380719,EBI-539478
SRPK2P783622EBI-6380719,EBI-593303

Protein-protein interaction databases

BioGridi116132. 35 interactions.
IntActiQ9BRL6. 14 interactions.

Structurei

Secondary structure

1
282
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 206Combined sources
Helixi27 – 348Combined sources
Turni35 – 373Combined sources
Beta strandi40 – 445Combined sources
Beta strandi49 – 513Combined sources
Beta strandi58 – 669Combined sources
Helixi67 – 759Combined sources
Beta strandi86 – 894Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DNMNMR-A8-97[»]
ProteinModelPortaliQ9BRL6.
SMRiQ9BRL6. Positions 2-103.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BRL6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 9279RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi83 – 205123Arg-richAdd
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00700000104403.
InParanoidiQ9BRL6.
KOiK12891.
OMAiHRESRYS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BRL6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCGRPPPDV DGMITLKVDN LTYRTSPDSL RRVFEKYGRV GDVYIPREPH
60 70 80 90 100
TKAPRGFAFV RFHDRRDAQD AEAAMDGAEL DGRELRVQVA RYGRRDLPRS
110 120 130 140 150
RQGEPRGRSR GGGYGRRSRS YGRRSRSPRR RHRSRSRGPS CSRSRSRSRY
160 170 180 190 200
RGSRYSRSPY SRSPYSRSRY SRSPYSRSRY RESRYGGSHY SSSGYSNSRY
210 220 230 240 250
SRYHSSRSHS KSGSSTSSRS ASTSKSSSAR RSKSSSVSRS RSRSRSSSMT
260 270 280
RSPPRVSKRK SKSRSRSKRP PKSPEEEGQM SS
Length:282
Mass (Da):32,288
Last modified:June 1, 2001 - v1
Checksum:iA6FB97C88247EE0C
GO
Isoform 2 (identifier: Q9BRL6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-120: Missing.

Note: No experimental confirmation available.
Show »
Length:275
Mass (Da):31,425
Checksum:iEDD1E65F9959D80B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1561S → G in AAH57783 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei114 – 1207Missing in isoform 2. 1 PublicationVSP_028026

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031165 Genomic DNA. Translation: AAK54350.1.
AF031166 mRNA. Translation: AAK54351.1.
AK023379 mRNA. Translation: BAG51186.1.
AK312514 mRNA. Translation: BAG35415.1.
CH471065 Genomic DNA. Translation: EAW66954.1.
BC057783 mRNA. Translation: AAH57783.1.
CCDSiCCDS73370.1. [Q9BRL6-1]
RefSeqiNP_115285.1. NM_032102.3. [Q9BRL6-1]
UniGeneiHs.476680.
Hs.703293.

Genome annotation databases

EnsembliENST00000587424; ENSP00000480140; ENSG00000263465. [Q9BRL6-1]
GeneIDi10929.
KEGGihsa:10929.
UCSCiuc031ybj.2. human. [Q9BRL6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031165 Genomic DNA. Translation: AAK54350.1.
AF031166 mRNA. Translation: AAK54351.1.
AK023379 mRNA. Translation: BAG51186.1.
AK312514 mRNA. Translation: BAG35415.1.
CH471065 Genomic DNA. Translation: EAW66954.1.
BC057783 mRNA. Translation: AAH57783.1.
CCDSiCCDS73370.1. [Q9BRL6-1]
RefSeqiNP_115285.1. NM_032102.3. [Q9BRL6-1]
UniGeneiHs.476680.
Hs.703293.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DNMNMR-A8-97[»]
ProteinModelPortaliQ9BRL6.
SMRiQ9BRL6. Positions 2-103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116132. 35 interactions.
IntActiQ9BRL6. 14 interactions.

PTM databases

iPTMnetiQ9BRL6.
PhosphoSiteiQ9BRL6.

Polymorphism and mutation databases

DMDMi74761217.

Proteomic databases

EPDiQ9BRL6.
MaxQBiQ9BRL6.
PeptideAtlasiQ9BRL6.
PRIDEiQ9BRL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000587424; ENSP00000480140; ENSG00000263465. [Q9BRL6-1]
GeneIDi10929.
KEGGihsa:10929.
UCSCiuc031ybj.2. human. [Q9BRL6-1]

Organism-specific databases

CTDi10929.
GeneCardsiSRSF8.
H-InvDBHIX0010038.
HGNCiHGNC:16988. SRSF8.
HPAiHPA049905.
MIMi603269. gene.
neXtProtiNX_Q9BRL6.
PharmGKBiPA165543687.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00700000104403.
InParanoidiQ9BRL6.
KOiK12891.
OMAiHRESRYS.

Miscellaneous databases

EvolutionaryTraceiQ9BRL6.
GenomeRNAii10929.
PROiQ9BRL6.
SOURCEiSearch...

Gene expression databases

CleanExiHS_SFRS2B.
ExpressionAtlasiQ9BRL6. baseline and differential.
GenevisibleiQ9BRL6. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of SRp46, a novel human SR splicing factor encoded by a PR264/SC35 retropseudogene."
    Soret J., Gattoni R., Guyon C., Sureau A., Popielarz M., Le Rouzic E., Dumon S., Apiou F., Dutrillaux B., Voss H., Ansorge W., Stevenin J., Perbal B.
    Mol. Cell. Biol. 18:4924-4934(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Placenta and Thymus.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Ovary and Thalamus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-273, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-26; SER-156; SER-158; SER-171; SER-173 AND SER-196, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.
  10. "Solution structure of RNA-binding domain in SRP46 splicing factor."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 8-97.

Entry informationi

Entry nameiSRSF8_HUMAN
AccessioniPrimary (citable) accession number: Q9BRL6
Secondary accession number(s): B2R6B8, Q6PF01, Q96TA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.