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Protein

Serine/arginine-rich splicing factor 8

Gene

SRSF8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA alternative splicing.1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000180771-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 8
Alternative name(s):
Pre-mRNA-splicing factor SRP46
Short name:
Splicing factor SRp46
Splicing factor, arginine/serine-rich 2B
Gene namesi
Name:SRSF8
Synonyms:SFRS2B, SRP46
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:16988. SRSF8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000263465.
PharmGKBiPA165543687.

Polymorphism and mutation databases

DMDMi74761217.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003044122 – 282Serine/arginine-rich splicing factor 8Add BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei26PhosphoserineCombined sources1
Modified residuei156PhosphoserineCombined sources1
Modified residuei158PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei196PhosphoserineCombined sources1
Modified residuei273PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BRL6.
MaxQBiQ9BRL6.
PeptideAtlasiQ9BRL6.
PRIDEiQ9BRL6.

PTM databases

iPTMnetiQ9BRL6.
PhosphoSitePlusiQ9BRL6.

Expressioni

Tissue specificityi

Strongly expressed in pancreas, spleen and prostate. Weakly expressed in lung, liver and thymus.1 Publication

Gene expression databases

CleanExiHS_SFRS2B.
ExpressionAtlasiQ9BRL6. baseline and differential.
GenevisibleiQ9BRL6. HS.

Organism-specific databases

HPAiHPA049905.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SRPK1Q96SB42EBI-6380719,EBI-539478
SRPK2P783622EBI-6380719,EBI-593303

Protein-protein interaction databases

BioGridi116132. 35 interactors.
IntActiQ9BRL6. 18 interactors.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 20Combined sources6
Helixi27 – 34Combined sources8
Turni35 – 37Combined sources3
Beta strandi40 – 44Combined sources5
Beta strandi49 – 51Combined sources3
Beta strandi58 – 66Combined sources9
Helixi67 – 75Combined sources9
Beta strandi86 – 89Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DNMNMR-A8-97[»]
ProteinModelPortaliQ9BRL6.
SMRiQ9BRL6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BRL6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 92RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi83 – 205Arg-richAdd BLAST123

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00700000104403.
InParanoidiQ9BRL6.
KOiK12891.
OMAiHRESRYS.
OrthoDBiEOG091G13K0.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BRL6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCGRPPPDV DGMITLKVDN LTYRTSPDSL RRVFEKYGRV GDVYIPREPH
60 70 80 90 100
TKAPRGFAFV RFHDRRDAQD AEAAMDGAEL DGRELRVQVA RYGRRDLPRS
110 120 130 140 150
RQGEPRGRSR GGGYGRRSRS YGRRSRSPRR RHRSRSRGPS CSRSRSRSRY
160 170 180 190 200
RGSRYSRSPY SRSPYSRSRY SRSPYSRSRY RESRYGGSHY SSSGYSNSRY
210 220 230 240 250
SRYHSSRSHS KSGSSTSSRS ASTSKSSSAR RSKSSSVSRS RSRSRSSSMT
260 270 280
RSPPRVSKRK SKSRSRSKRP PKSPEEEGQM SS
Length:282
Mass (Da):32,288
Last modified:June 1, 2001 - v1
Checksum:iA6FB97C88247EE0C
GO
Isoform 2 (identifier: Q9BRL6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-120: Missing.

Note: No experimental confirmation available.
Show »
Length:275
Mass (Da):31,425
Checksum:iEDD1E65F9959D80B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156S → G in AAH57783 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028026114 – 120Missing in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031165 Genomic DNA. Translation: AAK54350.1.
AF031166 mRNA. Translation: AAK54351.1.
AK023379 mRNA. Translation: BAG51186.1.
AK312514 mRNA. Translation: BAG35415.1.
CH471065 Genomic DNA. Translation: EAW66954.1.
BC057783 mRNA. Translation: AAH57783.1.
CCDSiCCDS73370.1. [Q9BRL6-1]
RefSeqiNP_115285.1. NM_032102.3. [Q9BRL6-1]
XP_016872631.1. XM_017017142.1. [Q9BRL6-1]
UniGeneiHs.476680.
Hs.703293.

Genome annotation databases

EnsembliENST00000587424; ENSP00000480140; ENSG00000263465. [Q9BRL6-1]
GeneIDi10929.
KEGGihsa:10929.
UCSCiuc031ybj.2. human. [Q9BRL6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031165 Genomic DNA. Translation: AAK54350.1.
AF031166 mRNA. Translation: AAK54351.1.
AK023379 mRNA. Translation: BAG51186.1.
AK312514 mRNA. Translation: BAG35415.1.
CH471065 Genomic DNA. Translation: EAW66954.1.
BC057783 mRNA. Translation: AAH57783.1.
CCDSiCCDS73370.1. [Q9BRL6-1]
RefSeqiNP_115285.1. NM_032102.3. [Q9BRL6-1]
XP_016872631.1. XM_017017142.1. [Q9BRL6-1]
UniGeneiHs.476680.
Hs.703293.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DNMNMR-A8-97[»]
ProteinModelPortaliQ9BRL6.
SMRiQ9BRL6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116132. 35 interactors.
IntActiQ9BRL6. 18 interactors.

PTM databases

iPTMnetiQ9BRL6.
PhosphoSitePlusiQ9BRL6.

Polymorphism and mutation databases

DMDMi74761217.

Proteomic databases

EPDiQ9BRL6.
MaxQBiQ9BRL6.
PeptideAtlasiQ9BRL6.
PRIDEiQ9BRL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000587424; ENSP00000480140; ENSG00000263465. [Q9BRL6-1]
GeneIDi10929.
KEGGihsa:10929.
UCSCiuc031ybj.2. human. [Q9BRL6-1]

Organism-specific databases

CTDi10929.
GeneCardsiSRSF8.
H-InvDBHIX0010038.
HGNCiHGNC:16988. SRSF8.
HPAiHPA049905.
MIMi603269. gene.
neXtProtiNX_Q9BRL6.
OpenTargetsiENSG00000263465.
PharmGKBiPA165543687.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00700000104403.
InParanoidiQ9BRL6.
KOiK12891.
OMAiHRESRYS.
OrthoDBiEOG091G13K0.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000180771-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9BRL6.
GenomeRNAii10929.
PROiQ9BRL6.
SOURCEiSearch...

Gene expression databases

CleanExiHS_SFRS2B.
ExpressionAtlasiQ9BRL6. baseline and differential.
GenevisibleiQ9BRL6. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF8_HUMAN
AccessioniPrimary (citable) accession number: Q9BRL6
Secondary accession number(s): B2R6B8, Q6PF01, Q96TA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.