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Protein

45 kDa calcium-binding protein

Gene

SDF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.By similarity
Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi111 – 1221PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi150 – 1612PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi209 – 2203PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi246 – 2574PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi291 – 3025PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi327 – 3386PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: BHF-UCL
  • identical protein binding Source: BHF-UCL

GO - Biological processi

  • calcium ion regulated exocytosis Source: BHF-UCL
  • cerebellum development Source: BHF-UCL
  • fat cell differentiation Source: BHF-UCL
  • response to ethanol Source: BHF-UCL
  • UV protection Source: BHF-UCL
  • zymogen granule exocytosis Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000078808-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
45 kDa calcium-binding protein
Short name:
Cab45
Alternative name(s):
Stromal cell-derived factor 4
Short name:
SDF-4
Gene namesi
Name:SDF4
Synonyms:CAB45
ORF Names:PSEC0034
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:24188. SDF4.

Subcellular locationi

Isoform 1 :
  • Golgi apparatus lumen By similarity
Isoform 5 :

GO - Cellular componenti

  • bleb Source: UniProtKB-SubCell
  • cytoplasm Source: BHF-UCL
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: HPA
  • Golgi lumen Source: BHF-UCL
  • late endosome Source: BHF-UCL
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257E → Q: Does not affect calcium-binding. 1 Publication1
Mutagenesisi302E → Q: Inhibits calcium-binding. 1 Publication1
Mutagenesisi338E → Q: Does not affect calcium-binding. 1 Publication1

Organism-specific databases

DisGeNETi51150.
OpenTargetsiENSG00000078808.
PharmGKBiPA142670940.

Polymorphism and mutation databases

BioMutaiSDF4.
DMDMi21263447.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
ChainiPRO_000000415637 – 36245 kDa calcium-binding proteinAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...)Sequence analysis1
Modified residuei99PhosphoserineCombined sources1
Modified residuei193PhosphothreonineCombined sources1
Modified residuei217PhosphothreonineCombined sources1
Modified residuei265PhosphothreonineCombined sources1
Modified residuei299PhosphothreonineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9BRK5.
MaxQBiQ9BRK5.
PaxDbiQ9BRK5.
PeptideAtlasiQ9BRK5.
PRIDEiQ9BRK5.
TopDownProteomicsiQ9BRK5-4. [Q9BRK5-4]

PTM databases

iPTMnetiQ9BRK5.
PhosphoSitePlusiQ9BRK5.

Expressioni

Tissue specificityi

Ubiquitous. Isoform 5 is expressed in pancreas.1 Publication

Gene expression databases

BgeeiENSG00000078808.
CleanExiHS_SDF4.
ExpressionAtlasiQ9BRK5. baseline and differential.
GenevisibleiQ9BRK5. HS.

Organism-specific databases

HPAiCAB015227.
HPA011249.

Interactioni

Subunit structurei

Isoform 5 interacts with STXBP1; the interaction is enhanced in presence of calcium. Isoform 5 interacts with STX3.1 Publication

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi119334. 84 interactors.
IntActiQ9BRK5. 39 interactors.
MINTiMINT-1186958.
STRINGi9606.ENSP00000353094.

Structurei

3D structure databases

ProteinModelPortaliQ9BRK5.
SMRiQ9BRK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 133EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini137 – 172EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini197 – 232EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini233 – 268EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Domaini278 – 313EF-hand 5PROSITE-ProRule annotationAdd BLAST36
Domaini314 – 349EF-hand 6PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 362Necessary for intracellular retention in Golgi apparatus lumenBy similarityAdd BLAST54

Domaini

Binds calcium via its EF-hands (By similarity). Isoform 5 binds calcium.By similarity

Sequence similaritiesi

Belongs to the CREC family.Curated
Contains 6 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4251. Eukaryota.
ENOG410XSH7. LUCA.
GeneTreeiENSGT00550000074546.
HOVERGENiHBG097344.
InParanoidiQ9BRK5.
KOiK19934.
OMAiMNEHNAL.
OrthoDBiEOG091G0GSA.
PhylomeDBiQ9BRK5.
TreeFamiTF314849.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 3 hits.
InterProiIPR027240. CAB45.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10827:SF51. PTHR10827:SF51. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 5 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BRK5-1) [UniParc]FASTAAdd to basket
Also known as: Cab45a, Cab45-G

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVWPWVAMAS RWGPLIGLAP CCLWLLGAVL LMDASARPAN HSSTRERVAN
60 70 80 90 100
REENEILPPD HLNGVKLEMD GHLNRGFHQE VFLGKDLGGF DEDAEPRRSR
110 120 130 140 150
RKLMVIFSKV DVNTDRKISA KEMQRWIMEK TAEHFQEAME ESKTHFRAVD
160 170 180 190 200
PDGDGHVSWD EYKVKFLASK GHSEKEVADA IRLNEELKVD EETQEVLENL
210 220 230 240 250
KDRWYQADSP PADLLLTEEE FLSFLHPEHS RGMLRFMVKE IVRDLDQDGD
260 270 280 290 300
KQLSVPEFIS LPVGTVENQQ GQDIDDNWVK DRKKEFEELI DSNHDGIVTA
310 320 330 340 350
EELESYMDPM NEYNALNEAK QMIAVADENQ NHHLEPEEVL KYSEFFTGSK
360
LVDYARSVHE EF
Length:362
Mass (Da):41,807
Last modified:June 1, 2001 - v1
Checksum:i440C6990149AAE2C
GO
Isoform 2 (identifier: Q9BRK5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Show »
Length:259
Mass (Da):30,197
Checksum:i3745A23BB8FF6D40
GO
Isoform 3 (identifier: Q9BRK5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     193-254: TQEVLENLKD...LDQDGDKQLS → SCAPLSTGSP...GDRPGPGPGR
     255-362: Missing.

Note: No experimental confirmation available.
Show »
Length:254
Mass (Da):27,826
Checksum:i77FACCE59B472125
GO
Isoform 4 (identifier: Q9BRK5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     193-202: TQEVLENLKD → RHGPPGPRAL
     203-362: Missing.

Note: No experimental confirmation available.
Show »
Length:202
Mass (Da):23,031
Checksum:i5F208F14504756F7
GO
Isoform 5 (identifier: Q9BRK5-5) [UniParc]FASTAAdd to basket
Also known as: Cab45b, Cab45-C

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: Missing.

Show »
Length:130
Mass (Da):15,139
Checksum:i22545D121BB56827
GO
Isoform 6 (identifier: Q9BRK5-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     305-362: SYMDPMNEYN...DYARSVHEEF → NVPTLPLQPI...PLARRATWTP

Show »
Length:348
Mass (Da):39,608
Checksum:i1832BC4741A9506A
GO

Sequence cautioni

The sequence AAL75950 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78H → Q in AAL75950 (Ref. 2) Curated1
Sequence conflicti86D → N in AAL75950 (Ref. 2) Curated1
Sequence conflicti89G → C in AAL75950 (Ref. 2) Curated1
Sequence conflicti92E → R in AAL75950 (Ref. 2) Curated1
Sequence conflicti94A → T in AAL75950 (Ref. 2) Curated1
Sequence conflicti97R → P in AAL75950 (Ref. 2) Curated1
Sequence conflicti117K → R in BAC11563 (PubMed:16303743).Curated1
Sequence conflicti133E → G in BAC11563 (PubMed:16303743).Curated1
Sequence conflicti150D → G in AAL75950 (Ref. 2) Curated1
Sequence conflicti188K → R in BAG37898 (PubMed:14702039).Curated1
Sequence conflicti193T → A in BAD96241 (Ref. 7) Curated1
Sequence conflicti234L → P in BAD96799 (Ref. 7) Curated1
Sequence conflicti281 – 282DR → KK in AAL75950 (Ref. 2) Curated2
Sequence conflicti286F → L in AAL75950 (Ref. 2) Curated1
Sequence conflicti321Q → R in BAD96799 (Ref. 7) Curated1
Sequence conflicti356R → S in AAL75950 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04865950N → D.Corresponds to variant rs12745364dbSNPEnsembl.1
Natural variantiVAR_035461148A → T in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs766752243dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0374841 – 232Missing in isoform 5. CuratedAdd BLAST232
Alternative sequenceiVSP_0374851 – 103Missing in isoform 2. 1 PublicationAdd BLAST103
Alternative sequenceiVSP_037486193 – 254TQEVL…DKQLS → SCAPLSTGSPGEPEGPLVPG GQPPCRPAADGGGVPVGPPP RAQPGNAQVHGEGDRPGPGP GR in isoform 3. 1 PublicationAdd BLAST62
Alternative sequenceiVSP_037487193 – 202TQEVLENLKD → RHGPPGPRAL in isoform 4. 1 Publication10
Alternative sequenceiVSP_037488203 – 362Missing in isoform 4. 1 PublicationAdd BLAST160
Alternative sequenceiVSP_037489255 – 362Missing in isoform 3. 1 PublicationAdd BLAST108
Alternative sequenceiVSP_040559305 – 362SYMDP…VHEEF → NVPTLPLQPIGTLNSHFVRL AAELGGGKATLTCAPLARRA TWTP in isoform 6. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L79912 mRNA. Translation: AAL40084.1.
AF132749 mRNA. Translation: AAL75950.1. Frameshift.
AF153686 mRNA. Translation: AAD51612.1.
AF178986 mRNA. Translation: AAF44350.1.
AK027277 mRNA. Translation: BAB55012.1.
AK299810 mRNA. Translation: BAG61683.1.
AK315517 mRNA. Translation: BAG37898.1.
AK075352 mRNA. Translation: BAC11563.1.
AK222521 mRNA. Translation: BAD96241.1.
AK223079 mRNA. Translation: BAD96799.1.
AL162741 Genomic DNA. Translation: CAI23251.1.
AL162741 Genomic DNA. Translation: CAI23252.1.
CH471183 Genomic DNA. Translation: EAW56272.1.
CH471183 Genomic DNA. Translation: EAW56273.1.
BC006211 mRNA. Translation: AAH06211.1.
BC007625 mRNA. Translation: AAH07625.1.
BC008917 mRNA. Translation: AAH08917.1.
BC011244 mRNA. Translation: AAH11244.1.
BC022375 mRNA. Translation: AAH22375.1.
CCDSiCCDS12.1. [Q9BRK5-6]
CCDS30553.1. [Q9BRK5-1]
RefSeqiNP_057260.2. NM_016176.3. [Q9BRK5-1]
NP_057631.1. NM_016547.2. [Q9BRK5-6]
XP_011539858.1. XM_011541556.1. [Q9BRK5-4]
UniGeneiHs.42806.

Genome annotation databases

EnsembliENST00000263741; ENSP00000263741; ENSG00000078808. [Q9BRK5-6]
ENST00000360001; ENSP00000353094; ENSG00000078808. [Q9BRK5-1]
GeneIDi51150.
KEGGihsa:51150.
UCSCiuc001adh.5. human. [Q9BRK5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L79912 mRNA. Translation: AAL40084.1.
AF132749 mRNA. Translation: AAL75950.1. Frameshift.
AF153686 mRNA. Translation: AAD51612.1.
AF178986 mRNA. Translation: AAF44350.1.
AK027277 mRNA. Translation: BAB55012.1.
AK299810 mRNA. Translation: BAG61683.1.
AK315517 mRNA. Translation: BAG37898.1.
AK075352 mRNA. Translation: BAC11563.1.
AK222521 mRNA. Translation: BAD96241.1.
AK223079 mRNA. Translation: BAD96799.1.
AL162741 Genomic DNA. Translation: CAI23251.1.
AL162741 Genomic DNA. Translation: CAI23252.1.
CH471183 Genomic DNA. Translation: EAW56272.1.
CH471183 Genomic DNA. Translation: EAW56273.1.
BC006211 mRNA. Translation: AAH06211.1.
BC007625 mRNA. Translation: AAH07625.1.
BC008917 mRNA. Translation: AAH08917.1.
BC011244 mRNA. Translation: AAH11244.1.
BC022375 mRNA. Translation: AAH22375.1.
CCDSiCCDS12.1. [Q9BRK5-6]
CCDS30553.1. [Q9BRK5-1]
RefSeqiNP_057260.2. NM_016176.3. [Q9BRK5-1]
NP_057631.1. NM_016547.2. [Q9BRK5-6]
XP_011539858.1. XM_011541556.1. [Q9BRK5-4]
UniGeneiHs.42806.

3D structure databases

ProteinModelPortaliQ9BRK5.
SMRiQ9BRK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119334. 84 interactors.
IntActiQ9BRK5. 39 interactors.
MINTiMINT-1186958.
STRINGi9606.ENSP00000353094.

PTM databases

iPTMnetiQ9BRK5.
PhosphoSitePlusiQ9BRK5.

Polymorphism and mutation databases

BioMutaiSDF4.
DMDMi21263447.

Proteomic databases

EPDiQ9BRK5.
MaxQBiQ9BRK5.
PaxDbiQ9BRK5.
PeptideAtlasiQ9BRK5.
PRIDEiQ9BRK5.
TopDownProteomicsiQ9BRK5-4. [Q9BRK5-4]

Protocols and materials databases

DNASUi51150.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263741; ENSP00000263741; ENSG00000078808. [Q9BRK5-6]
ENST00000360001; ENSP00000353094; ENSG00000078808. [Q9BRK5-1]
GeneIDi51150.
KEGGihsa:51150.
UCSCiuc001adh.5. human. [Q9BRK5-1]

Organism-specific databases

CTDi51150.
DisGeNETi51150.
GeneCardsiSDF4.
HGNCiHGNC:24188. SDF4.
HPAiCAB015227.
HPA011249.
MIMi614282. gene.
neXtProtiNX_Q9BRK5.
OpenTargetsiENSG00000078808.
PharmGKBiPA142670940.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4251. Eukaryota.
ENOG410XSH7. LUCA.
GeneTreeiENSGT00550000074546.
HOVERGENiHBG097344.
InParanoidiQ9BRK5.
KOiK19934.
OMAiMNEHNAL.
OrthoDBiEOG091G0GSA.
PhylomeDBiQ9BRK5.
TreeFamiTF314849.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000078808-MONOMER.

Miscellaneous databases

ChiTaRSiSDF4. human.
GeneWikiiSDF4.
GenomeRNAii51150.
PROiQ9BRK5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000078808.
CleanExiHS_SDF4.
ExpressionAtlasiQ9BRK5. baseline and differential.
GenevisibleiQ9BRK5. HS.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 3 hits.
InterProiIPR027240. CAB45.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10827:SF51. PTHR10827:SF51. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 5 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAB45_HUMAN
AccessioniPrimary (citable) accession number: Q9BRK5
Secondary accession number(s): B1AME5
, B1AME6, B2RDF1, B4DSM1, Q53G52, Q53HQ9, Q8NBQ3, Q96AA1, Q9NZP7, Q9UN53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.