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Protein

Matrix-remodeling-associated protein 8

Gene

MXRA8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the maturation and maintenance of blood-brain barrier.By similarity

GO - Biological processi

  • establishment of glial blood-brain barrier Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix-remodeling-associated protein 8
Alternative name(s):
Limitrin
Gene namesi
Name:MXRA8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:7542. MXRA8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 341322ExtracellularSequence analysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence analysisAdd
BLAST
Topological domaini363 – 44280CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31343.

Polymorphism and mutation databases

DMDMi74761214.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 442423Matrix-remodeling-associated protein 8PRO_0000298665Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 137PROSITE-ProRule annotation
Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence analysis
Disulfide bondi186 ↔ 272PROSITE-ProRule annotation
Modified residuei228 – 2281Phosphoserine; by FAM20C1 Publication
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9BRK3.
MaxQBiQ9BRK3.
PaxDbiQ9BRK3.
PRIDEiQ9BRK3.

PTM databases

iPTMnetiQ9BRK3.
PhosphoSiteiQ9BRK3.

Expressioni

Gene expression databases

BgeeiQ9BRK3.
CleanExiHS_MXRA8.
GenevisibleiQ9BRK3. HS.

Organism-specific databases

HPAiHPA053958.
HPA055780.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000307887.

Structurei

3D structure databases

ProteinModelPortaliQ9BRK3.
SMRiQ9BRK3. Positions 41-141, 174-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 157129Ig-like V-type 1Add
BLAST
Domaini160 – 288129Ig-like V-type 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi364 – 3674Poly-Arg

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJVY. Eukaryota.
ENOG41113W9. LUCA.
GeneTreeiENSGT00390000001509.
HOGENOMiHOG000065729.
HOVERGENiHBG058527.
InParanoidiQ9BRK3.
OMAiLLTCVNR.
OrthoDBiEOG71RXJW.
PhylomeDBiQ9BRK3.
TreeFamiTF332884.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 2 hits.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BRK3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALPSRILLW KLVLLQSSAV LLHSGSSVPA AAGSSVVSES AVSWEAGARA
60 70 80 90 100
VLRCQSPRMV WTQDRLHDRQ RVLHWDLRGP GGGPARRLLD LYSAGEQRVY
110 120 130 140 150
EARDRGRLEL SASAFDDGNF SLLIRAVEET DAGLYTCNLH HHYCHLYESL
160 170 180 190 200
AVRLEVTDGP PATPAYWDGE KEVLAVARGA PALLTCVNRG HVWTDRHVEE
210 220 230 240 250
AQQVVHWDRQ PPGVPHDRAD RLLDLYASGE RRAYGPLFLR DRVAVGADAF
260 270 280 290 300
ERGDFSLRIE PLEVADEGTY SCHLHHHYCG LHERRVFHLT VAEPHAEPPP
310 320 330 340 350
RGSPGNGSSH SGAPGPDPTL ARGHNVINVI VPESRAHFFQ QLGYVLATLL
360 370 380 390 400
LFILLLVTVL LAARRRRGGY EYSDQKSGKS KGKDVNLAEF AVAAGDQMLY
410 420 430 440
RSEDIQLDYK NNILKERAEL AHSPLPAKYI DLDKGFRKEN CK
Length:442
Mass (Da):49,132
Last modified:June 1, 2001 - v1
Checksum:iB08F89D726222CA1
GO
Isoform 2 (identifier: Q9BRK3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     407-407: L → LASSPPT
     435-442: GFRKENCK → DPSGLCPLGA

Show »
Length:450
Mass (Da):49,620
Checksum:i93865CBB68AC1C04
GO
Isoform 3 (identifier: Q9BRK3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MALPSRILLWKLVLLQS → MIRCAATG

Note: No experimental confirmation available.
Show »
Length:433
Mass (Da):47,972
Checksum:iAFECF654C299D6D6
GO
Isoform 4 (identifier: Q9BRK3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     25-125: Missing.

Note: No experimental confirmation available.
Show »
Length:341
Mass (Da):38,070
Checksum:i788EDD861A10CCF5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4051I → V in BAB55010 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti396 – 3961D → N.1 Publication
Corresponds to variant rs150058708 [ dbSNP | Ensembl ].
VAR_070893

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1717MALPS…VLLQS → MIRCAATG in isoform 3. 1 PublicationVSP_054528Add
BLAST
Alternative sequencei25 – 125101Missing in isoform 4. 1 PublicationVSP_054529Add
BLAST
Alternative sequencei407 – 4071L → LASSPPT in isoform 2. 1 PublicationVSP_027448
Alternative sequencei435 – 4428GFRKENCK → DPSGLCPLGA in isoform 2. 1 PublicationVSP_027449

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052096 mRNA. Translation: BAD05132.1.
AK027269 mRNA. Translation: BAB55010.1.
AK095966 mRNA. Translation: BAG53178.1.
AK293442 mRNA. Translation: BAG56945.1.
AL139287 Genomic DNA. Translation: CAI23188.1.
AL139287 Genomic DNA. Translation: CAI23189.1.
BC006213 mRNA. Translation: AAH06213.1.
BC017312 mRNA. Translation: AAH17312.1.
CCDSiCCDS24.1. [Q9BRK3-1]
CCDS59950.1. [Q9BRK3-4]
CCDS59951.1. [Q9BRK3-2]
CCDS59952.1. [Q9BRK3-3]
RefSeqiNP_001269511.1. NM_001282582.1. [Q9BRK3-1]
NP_001269512.1. NM_001282583.1. [Q9BRK3-3]
NP_001269513.1. NM_001282584.1. [Q9BRK3-4]
NP_001269514.1. NM_001282585.1. [Q9BRK3-2]
NP_115724.1. NM_032348.3. [Q9BRK3-1]
UniGeneiHs.515687.

Genome annotation databases

EnsembliENST00000309212; ENSP00000307887; ENSG00000162576. [Q9BRK3-1]
ENST00000342753; ENSP00000344998; ENSG00000162576. [Q9BRK3-4]
ENST00000445648; ENSP00000399229; ENSG00000162576. [Q9BRK3-2]
ENST00000477278; ENSP00000436135; ENSG00000162576. [Q9BRK3-3]
GeneIDi54587.
KEGGihsa:54587.
UCSCiuc001aew.5. human. [Q9BRK3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052096 mRNA. Translation: BAD05132.1.
AK027269 mRNA. Translation: BAB55010.1.
AK095966 mRNA. Translation: BAG53178.1.
AK293442 mRNA. Translation: BAG56945.1.
AL139287 Genomic DNA. Translation: CAI23188.1.
AL139287 Genomic DNA. Translation: CAI23189.1.
BC006213 mRNA. Translation: AAH06213.1.
BC017312 mRNA. Translation: AAH17312.1.
CCDSiCCDS24.1. [Q9BRK3-1]
CCDS59950.1. [Q9BRK3-4]
CCDS59951.1. [Q9BRK3-2]
CCDS59952.1. [Q9BRK3-3]
RefSeqiNP_001269511.1. NM_001282582.1. [Q9BRK3-1]
NP_001269512.1. NM_001282583.1. [Q9BRK3-3]
NP_001269513.1. NM_001282584.1. [Q9BRK3-4]
NP_001269514.1. NM_001282585.1. [Q9BRK3-2]
NP_115724.1. NM_032348.3. [Q9BRK3-1]
UniGeneiHs.515687.

3D structure databases

ProteinModelPortaliQ9BRK3.
SMRiQ9BRK3. Positions 41-141, 174-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000307887.

PTM databases

iPTMnetiQ9BRK3.
PhosphoSiteiQ9BRK3.

Polymorphism and mutation databases

DMDMi74761214.

Proteomic databases

EPDiQ9BRK3.
MaxQBiQ9BRK3.
PaxDbiQ9BRK3.
PRIDEiQ9BRK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309212; ENSP00000307887; ENSG00000162576. [Q9BRK3-1]
ENST00000342753; ENSP00000344998; ENSG00000162576. [Q9BRK3-4]
ENST00000445648; ENSP00000399229; ENSG00000162576. [Q9BRK3-2]
ENST00000477278; ENSP00000436135; ENSG00000162576. [Q9BRK3-3]
GeneIDi54587.
KEGGihsa:54587.
UCSCiuc001aew.5. human. [Q9BRK3-1]

Organism-specific databases

CTDi54587.
GeneCardsiMXRA8.
HGNCiHGNC:7542. MXRA8.
HPAiHPA053958.
HPA055780.
neXtProtiNX_Q9BRK3.
PharmGKBiPA31343.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJVY. Eukaryota.
ENOG41113W9. LUCA.
GeneTreeiENSGT00390000001509.
HOGENOMiHOG000065729.
HOVERGENiHBG058527.
InParanoidiQ9BRK3.
OMAiLLTCVNR.
OrthoDBiEOG71RXJW.
PhylomeDBiQ9BRK3.
TreeFamiTF332884.

Miscellaneous databases

GenomeRNAii54587.
NextBioi35469956.
PROiQ9BRK3.

Gene expression databases

BgeeiQ9BRK3.
CleanExiHS_MXRA8.
GenevisibleiQ9BRK3. HS.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 2 hits.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Limitrin, a novel immunoglobulin superfamily protein localized to glia limitans formed by astrocyte endfeet."
    Yonezawa T., Ohtsuka A., Yoshitaka T., Hirano S., Nomoto H., Yamamoto K., Ninomiya Y.
    Glia 44:190-204(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4), VARIANT ASN-396.
    Tissue: Embryo.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye and Pancreas.
  5. Cited for: PHOSPHORYLATION AT SER-228.

Entry informationi

Entry nameiMXRA8_HUMAN
AccessioniPrimary (citable) accession number: Q9BRK3
Secondary accession number(s): B3KTR6
, B4DE34, Q5TA39, Q96KC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.