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Q9BRG1

- VPS25_HUMAN

UniProt

Q9BRG1 - VPS25_HUMAN

Protein

Vacuolar protein-sorting-associated protein 25

Gene

VPS25

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProt
    2. protein homodimerization activity Source: UniProt

    GO - Biological processi

    1. endosomal transport Source: Reactome
    2. membrane organization Source: Reactome
    3. protein transport Source: UniProtKB-KW
    4. regulation of transcription, DNA-templated Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transcription, Transcription regulation, Transport

    Enzyme and pathway databases

    ReactomeiREACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein-sorting-associated protein 25
    Short name:
    hVps25
    Alternative name(s):
    Dermal papilla-derived protein 9
    ELL-associated protein of 20 kDa
    ESCRT-II complex subunit VPS25
    Gene namesi
    Name:VPS25
    Synonyms:DERP9, EAP20
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:28122. VPS25.

    Subcellular locationi

    Cytoplasm. Endosome membrane. Nucleusnucleoplasm
    Note: Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endosome membrane Source: UniProt
    3. extracellular vesicular exosome Source: UniProt
    4. mitochondrion Source: Ensembl
    5. nucleolus Source: HPA
    6. nucleoplasm Source: UniProtKB-SubCell
    7. nucleus Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi124 – 1241V → E: Abolishes binding to CHMP6. 1 Publication
    Mutagenesisi126 – 1261T → K: Abolishes binding to CHMP6. 1 Publication

    Organism-specific databases

    PharmGKBiPA142670614.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 176176Vacuolar protein-sorting-associated protein 25PRO_0000215216Add
    BLAST

    Proteomic databases

    MaxQBiQ9BRG1.
    PaxDbiQ9BRG1.
    PeptideAtlasiQ9BRG1.
    PRIDEiQ9BRG1.

    PTM databases

    PhosphoSiteiQ9BRG1.

    Expressioni

    Tissue specificityi

    Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.1 Publication

    Gene expression databases

    ArrayExpressiQ9BRG1.
    BgeeiQ9BRG1.
    CleanExiHS_VPS25.
    GenevestigatoriQ9BRG1.

    Organism-specific databases

    HPAiCAB004978.
    HPA052217.
    HPA057284.

    Interactioni

    Subunit structurei

    Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 By similarity. Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and 2 copies of VPS25. Interacts with CFTR; the interaction requires misfolded CFTR. Interacts (via C-terminal half) with the ESCRT-III subunit CHMP6 (via N-terminal half).By similarity7 Publications

    Protein-protein interaction databases

    BioGridi124039. 28 interactions.
    IntActiQ9BRG1. 4 interactions.
    MINTiMINT-5003482.
    STRINGi9606.ENSP00000253794.

    Structurei

    Secondary structure

    1
    176
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 124
    Helixi14 – 174
    Helixi23 – 4422
    Beta strandi48 – 503
    Helixi51 – 555
    Beta strandi60 – 623
    Turni63 – 664
    Helixi71 – 8414
    Beta strandi86 – 894
    Beta strandi91 – 999
    Helixi104 – 11613
    Turni117 – 1193
    Beta strandi123 – 1253
    Helixi127 – 1326
    Turni135 – 1384
    Turni140 – 1434
    Helixi146 – 15813
    Beta strandi161 – 1655
    Turni167 – 1693
    Beta strandi172 – 1754

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZMEX-ray2.90C/D1-102[»]
    3CUQX-ray2.61C/D1-176[»]
    3HTUX-ray2.00A/C/E/G102-176[»]
    ProteinModelPortaliQ9BRG1.
    SMRiQ9BRG1. Positions 4-176.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9BRG1.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the VPS25 family.Curated

    Phylogenomic databases

    eggNOGiNOG270942.
    HOGENOMiHOG000191978.
    HOVERGENiHBG080015.
    InParanoidiQ9BRG1.
    KOiK12189.
    OMAiPPFFTIQ.
    OrthoDBiEOG7BGHN9.
    PhylomeDBiQ9BRG1.
    TreeFamiTF317731.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.10.10.570. 1 hit.
    InterProiIPR008570. ESCRT-II_cplx_vps25-sub.
    IPR014041. ESCRT-II_cplx_Vps25-sub_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR13149. PTHR13149. 1 hit.
    PfamiPF05871. ESCRT-II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9BRG1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT    50
    VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM 100
    WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL 150
    RALQALQQEH KAEIITVSDG RGVKFF 176
    Length:176
    Mass (Da):20,748
    Last modified:June 1, 2001 - v1
    Checksum:i34963A53C3DA4DD5
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti76 – 761I → V.
    Corresponds to variant rs34494804 [ dbSNP | Ensembl ].
    VAR_048940

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB014763 mRNA. Translation: BAB87804.1.
    AK312092 mRNA. Translation: BAG35028.1.
    CH471152 Genomic DNA. Translation: EAW60879.1.
    BC006282 mRNA. Translation: AAH06282.1.
    CCDSiCCDS11438.1.
    RefSeqiNP_115729.1. NM_032353.3.
    UniGeneiHs.500165.

    Genome annotation databases

    EnsembliENST00000253794; ENSP00000253794; ENSG00000131475.
    GeneIDi84313.
    KEGGihsa:84313.
    UCSCiuc002ibi.3. human.

    Polymorphism databases

    DMDMi73920459.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB014763 mRNA. Translation: BAB87804.1 .
    AK312092 mRNA. Translation: BAG35028.1 .
    CH471152 Genomic DNA. Translation: EAW60879.1 .
    BC006282 mRNA. Translation: AAH06282.1 .
    CCDSi CCDS11438.1.
    RefSeqi NP_115729.1. NM_032353.3.
    UniGenei Hs.500165.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZME X-ray 2.90 C/D 1-102 [» ]
    3CUQ X-ray 2.61 C/D 1-176 [» ]
    3HTU X-ray 2.00 A/C/E/G 102-176 [» ]
    ProteinModelPortali Q9BRG1.
    SMRi Q9BRG1. Positions 4-176.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124039. 28 interactions.
    IntActi Q9BRG1. 4 interactions.
    MINTi MINT-5003482.
    STRINGi 9606.ENSP00000253794.

    PTM databases

    PhosphoSitei Q9BRG1.

    Polymorphism databases

    DMDMi 73920459.

    Proteomic databases

    MaxQBi Q9BRG1.
    PaxDbi Q9BRG1.
    PeptideAtlasi Q9BRG1.
    PRIDEi Q9BRG1.

    Protocols and materials databases

    DNASUi 84313.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000253794 ; ENSP00000253794 ; ENSG00000131475 .
    GeneIDi 84313.
    KEGGi hsa:84313.
    UCSCi uc002ibi.3. human.

    Organism-specific databases

    CTDi 84313.
    GeneCardsi GC17P040925.
    HGNCi HGNC:28122. VPS25.
    HPAi CAB004978.
    HPA052217.
    HPA057284.
    MIMi 610907. gene.
    neXtProti NX_Q9BRG1.
    PharmGKBi PA142670614.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG270942.
    HOGENOMi HOG000191978.
    HOVERGENi HBG080015.
    InParanoidi Q9BRG1.
    KOi K12189.
    OMAi PPFFTIQ.
    OrthoDBi EOG7BGHN9.
    PhylomeDBi Q9BRG1.
    TreeFami TF317731.

    Enzyme and pathway databases

    Reactomei REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).

    Miscellaneous databases

    EvolutionaryTracei Q9BRG1.
    GeneWikii VPS25.
    GenomeRNAii 84313.
    NextBioi 74014.
    PROi Q9BRG1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BRG1.
    Bgeei Q9BRG1.
    CleanExi HS_VPS25.
    Genevestigatori Q9BRG1.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.10.10.570. 1 hit.
    InterProi IPR008570. ESCRT-II_cplx_vps25-sub.
    IPR014041. ESCRT-II_cplx_Vps25-sub_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR13149. PTHR13149. 1 hit.
    Pfami PF05871. ESCRT-II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a dermal papilla derived gene."
      Ikeda A., Ukai Y., Yamashita M., Yoshimoto M.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Hair follicle dermal papilla.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.
    5. Cited for: INTERACTION WITH SNF8; VPS36 AND CHMP6.
    6. "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins."
      Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D.
      Proc. Natl. Acad. Sci. U.S.A. 100:12414-12419(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ESCRT-II COMPLEX, INTERACTION WITH VPS36; SNF8 AND CHMP6.
    7. Erratum
      Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D.
      Proc. Natl. Acad. Sci. U.S.A. 100:152845-152845(2003)
    8. "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting."
      Yorikawa C., Shibata H., Waguri S., Hatta K., Horii M., Katoh K., Kobayashi T., Uchiyama Y., Maki M.
      Biochem. J. 387:17-26(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CHMP6.
    9. "Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes."
      Sharma M., Pampinella F., Nemes C., Benharouga M., So J., Du K., Bache K.G., Papsin B., Zerangue N., Stenmark H., Lukacs G.L.
      J. Cell Biol. 164:923-933(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MISFOLDED CFTR.
    10. "Genetic structure and evolution of the Vps25 family, a yeast ESCRT-II component."
      Slater R., Bishop N.E.
      BMC Evol. Biol. 6:59-59(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    11. "Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release."
      Langelier C., von Schwedler U.K., Fisher R.D., De Domenico I., White P.L., Hill C.P., Kaplan J., Ward D., Sundquist W.I.
      J. Virol. 80:9465-9480(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNF8; VPS36 AND CHMP6, SUBCELLULAR LOCATION.
    12. "Avian sarcoma virus and human immunodeficiency virus, type 1 use different subsets of ESCRT proteins to facilitate the budding process."
      Pincetic A., Medina G., Carter C., Leis J.
      J. Biol. Chem. 283:29822-29830(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex."
      Im Y.J., Hurley J.H.
      Dev. Cell 14:902-913(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.61 ANGSTROMS) IN COMPLEX WITH SNF8 AND VPS36.
    15. "Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis."
      Im Y.J., Wollert T., Boura E., Hurley J.H.
      Dev. Cell 17:234-243(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 102-176 IN COMPLEX WITH CHMP6, MUTAGENESIS OF VAL-124 AND THR-126.

    Entry informationi

    Entry nameiVPS25_HUMAN
    AccessioniPrimary (citable) accession number: Q9BRG1
    Secondary accession number(s): B2R581
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3