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Protein

Vacuolar protein-sorting-associated protein 25

Gene

VPS25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein N-terminus binding Source: UniProtKB
  • structural molecule activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131475-MONOMER.
ReactomeiR-HSA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 25
Short name:
hVps25
Alternative name(s):
Dermal papilla-derived protein 9
ELL-associated protein of 20 kDa
ESCRT-II complex subunit VPS25
Gene namesi
Name:VPS25
Synonyms:DERP9, EAP20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:28122. VPS25.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • endosome membrane Source: UniProtKB
  • ESCRT II complex Source: ParkinsonsUK-UCL
  • extracellular exosome Source: UniProtKB
  • nucleolus Source: HPA
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124V → E: Abolishes binding to CHMP6. 1 Publication1
Mutagenesisi126T → K: Abolishes binding to CHMP6. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000131475.
PharmGKBiPA142670614.

Polymorphism and mutation databases

DMDMi73920459.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152161 – 176Vacuolar protein-sorting-associated protein 25Add BLAST176

Proteomic databases

EPDiQ9BRG1.
MaxQBiQ9BRG1.
PaxDbiQ9BRG1.
PeptideAtlasiQ9BRG1.
PRIDEiQ9BRG1.

PTM databases

iPTMnetiQ9BRG1.
PhosphoSitePlusiQ9BRG1.

Expressioni

Tissue specificityi

Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.1 Publication

Gene expression databases

BgeeiENSG00000131475.
CleanExiHS_VPS25.
ExpressionAtlasiQ9BRG1. baseline and differential.
GenevisibleiQ9BRG1. HS.

Organism-specific databases

HPAiCAB004978.
HPA052217.
HPA057284.

Interactioni

Subunit structurei

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and 2 copies of VPS25. Interacts with CFTR; the interaction requires misfolded CFTR. Interacts (via C-terminal half) with the ESCRT-III subunit CHMP6 (via N-terminal half).By similarity7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BEND5Q7L4P63EBI-741945,EBI-724373
CHMP6Q96FZ74EBI-741945,EBI-1049648
CRLF3Q8IUI85EBI-741945,EBI-2872414
RELQ048643EBI-741945,EBI-307352
SNF8Q96H2011EBI-741945,EBI-747719
TRIM27P143735EBI-741945,EBI-719493

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124039. 32 interactors.
IntActiQ9BRG1. 13 interactors.
MINTiMINT-5003482.
STRINGi9606.ENSP00000253794.

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 12Combined sources4
Helixi14 – 17Combined sources4
Helixi23 – 44Combined sources22
Beta strandi48 – 50Combined sources3
Helixi51 – 55Combined sources5
Beta strandi60 – 62Combined sources3
Turni63 – 66Combined sources4
Helixi71 – 84Combined sources14
Beta strandi86 – 89Combined sources4
Beta strandi91 – 99Combined sources9
Helixi104 – 116Combined sources13
Turni117 – 119Combined sources3
Beta strandi123 – 125Combined sources3
Helixi127 – 132Combined sources6
Turni135 – 138Combined sources4
Turni140 – 143Combined sources4
Helixi146 – 158Combined sources13
Beta strandi161 – 165Combined sources5
Turni167 – 169Combined sources3
Beta strandi172 – 175Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZMEX-ray2.90C/D1-102[»]
3CUQX-ray2.61C/D1-176[»]
3HTUX-ray2.00A/C/E/G102-176[»]
ProteinModelPortaliQ9BRG1.
SMRiQ9BRG1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BRG1.

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS25 family.Curated

Phylogenomic databases

eggNOGiKOG4068. Eukaryota.
ENOG4111F6Y. LUCA.
GeneTreeiENSGT00390000014892.
HOGENOMiHOG000191978.
HOVERGENiHBG080015.
InParanoidiQ9BRG1.
KOiK12189.
OMAiKYLRHTN.
OrthoDBiEOG091G0PIU.
PhylomeDBiQ9BRG1.
TreeFamiTF317731.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.570. 1 hit.
InterProiIPR008570. ESCRT-II_cplx_vps25-sub.
IPR014041. ESCRT-II_cplx_Vps25-sub_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13149. PTHR13149. 1 hit.
PfamiPF05871. ESCRT-II. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9BRG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT
60 70 80 90 100
VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM
110 120 130 140 150
WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL
160 170
RALQALQQEH KAEIITVSDG RGVKFF
Length:176
Mass (Da):20,748
Last modified:June 1, 2001 - v1
Checksum:i34963A53C3DA4DD5
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04894076I → V.Corresponds to variant rs34494804dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014763 mRNA. Translation: BAB87804.1.
AK312092 mRNA. Translation: BAG35028.1.
CH471152 Genomic DNA. Translation: EAW60879.1.
BC006282 mRNA. Translation: AAH06282.1.
CCDSiCCDS11438.1.
RefSeqiNP_115729.1. NM_032353.3.
UniGeneiHs.500165.

Genome annotation databases

EnsembliENST00000253794; ENSP00000253794; ENSG00000131475.
GeneIDi84313.
KEGGihsa:84313.
UCSCiuc002ibi.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014763 mRNA. Translation: BAB87804.1.
AK312092 mRNA. Translation: BAG35028.1.
CH471152 Genomic DNA. Translation: EAW60879.1.
BC006282 mRNA. Translation: AAH06282.1.
CCDSiCCDS11438.1.
RefSeqiNP_115729.1. NM_032353.3.
UniGeneiHs.500165.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZMEX-ray2.90C/D1-102[»]
3CUQX-ray2.61C/D1-176[»]
3HTUX-ray2.00A/C/E/G102-176[»]
ProteinModelPortaliQ9BRG1.
SMRiQ9BRG1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124039. 32 interactors.
IntActiQ9BRG1. 13 interactors.
MINTiMINT-5003482.
STRINGi9606.ENSP00000253794.

PTM databases

iPTMnetiQ9BRG1.
PhosphoSitePlusiQ9BRG1.

Polymorphism and mutation databases

DMDMi73920459.

Proteomic databases

EPDiQ9BRG1.
MaxQBiQ9BRG1.
PaxDbiQ9BRG1.
PeptideAtlasiQ9BRG1.
PRIDEiQ9BRG1.

Protocols and materials databases

DNASUi84313.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253794; ENSP00000253794; ENSG00000131475.
GeneIDi84313.
KEGGihsa:84313.
UCSCiuc002ibi.5. human.

Organism-specific databases

CTDi84313.
GeneCardsiVPS25.
HGNCiHGNC:28122. VPS25.
HPAiCAB004978.
HPA052217.
HPA057284.
MIMi610907. gene.
neXtProtiNX_Q9BRG1.
OpenTargetsiENSG00000131475.
PharmGKBiPA142670614.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4068. Eukaryota.
ENOG4111F6Y. LUCA.
GeneTreeiENSGT00390000014892.
HOGENOMiHOG000191978.
HOVERGENiHBG080015.
InParanoidiQ9BRG1.
KOiK12189.
OMAiKYLRHTN.
OrthoDBiEOG091G0PIU.
PhylomeDBiQ9BRG1.
TreeFamiTF317731.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131475-MONOMER.
ReactomeiR-HSA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

ChiTaRSiVPS25. human.
EvolutionaryTraceiQ9BRG1.
GeneWikiiVPS25.
GenomeRNAii84313.
PROiQ9BRG1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131475.
CleanExiHS_VPS25.
ExpressionAtlasiQ9BRG1. baseline and differential.
GenevisibleiQ9BRG1. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.570. 1 hit.
InterProiIPR008570. ESCRT-II_cplx_vps25-sub.
IPR014041. ESCRT-II_cplx_Vps25-sub_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13149. PTHR13149. 1 hit.
PfamiPF05871. ESCRT-II. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiVPS25_HUMAN
AccessioniPrimary (citable) accession number: Q9BRG1
Secondary accession number(s): B2R581
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.