Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase CPPED1

Gene

CPPED1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase that dephosphorylates AKT family kinase specifically at 'Ser-473', blocking cell cycle progression and promoting cell apoptosis. May play an inhibitory role in glucose uptake by adipocytes.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Divalent metal cation 1By similarity
Metal bindingi90 – 901Divalent metal cation 1By similarity
Metal bindingi90 – 901Divalent metal cation 2By similarity
Metal bindingi127 – 1271Divalent metal cation 2By similarity
Metal bindingi247 – 2471Divalent metal cation 2By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase CPPED1 (EC:3.1.3.16)
Alternative name(s):
Calcineurin-like phosphoesterase domain-containing protein 1
Complete S-transactivated protein 1
Gene namesi
Name:CPPED1
Synonyms:CSTP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:25632. CPPED1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164718110.

Polymorphism and mutation databases

BioMutaiCPPED1.
DMDMi317373448.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Serine/threonine-protein phosphatase CPPED1PRO_0000320556Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BRF8.
MaxQBiQ9BRF8.
PaxDbiQ9BRF8.
PeptideAtlasiQ9BRF8.
PRIDEiQ9BRF8.

PTM databases

iPTMnetiQ9BRF8.
PhosphoSiteiQ9BRF8.

Expressioni

Tissue specificityi

Expressed in subcutaneous adipose tissue.1 Publication

Developmental stagei

Expression levels are slightly decreased in subcutaneous adipose tissue following weight loss. Expression levels may be not affected by preadipocyte differentiation.1 Publication

Inductioni

Transactivated by the large envelope protein of the hepatitis B virus (HBV).1 Publication

Gene expression databases

BgeeiQ9BRF8.
GenevisibleiQ9BRF8. HS.

Organism-specific databases

HPAiHPA040938.

Interactioni

Protein-protein interaction databases

BioGridi120595. 4 interactions.
IntActiQ9BRF8. 1 interaction.
STRINGi9606.ENSP00000371193.

Structurei

3D structure databases

ProteinModelPortaliQ9BRF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni47 – 250204CatalyticAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IP70. Eukaryota.
ENOG410ZI1D. LUCA.
GeneTreeiENSGT00390000008676.
HOGENOMiHOG000291782.
InParanoidiQ9BRF8.
OMAiGCQLGED.
OrthoDBiEOG7QRQW4.
PhylomeDBiQ9BRF8.
TreeFamiTF329406.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BRF8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAAEAGGVF HRARGRTLAA FPAEKESEWK GPFYFILGAD PQFGLIKAWS
60 70 80 90 100
TGDCDNGGDE WEQEIRLTEQ AVQAINKLNP KPKFFVLCGD LIHAMPGKPW
110 120 130 140 150
RTEQTEDLKR VLRAVDRAIP LVLVSGNHDI GNTPTAETVE EFCRTWGDDY
160 170 180 190 200
FSFWVGGVLF LVLNSQFYEN PSKCPSLKQA QDQWLDEQLS IARQRHCQHA
210 220 230 240 250
IVFQHIPLFL ESIDEDDDYY FNLSKSTRKK LADKFIHAGV KVVFSGHYHR
260 270 280 290 300
NAGGTYQNLD MVVSSAIGCQ LGRDPHGLRV VVVTAEKIVH RYYSLDELSE
310
KGIEDDLMDL IKKK
Length:314
Mass (Da):35,548
Last modified:January 11, 2011 - v3
Checksum:iD2DC770103053DA7
GO
Isoform 2 (identifier: Q9BRF8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-238: Missing.

Note: No experimental confirmation available.
Show »
Length:172
Mass (Da):19,036
Checksum:i63E35E20517FFCF7
GO
Isoform 3 (identifier: Q9BRF8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-122: GKPWRTEQTEDLKRVLRAVDRAIPLV → AGQRPPRAPSRGGHRRENCSPILQSR
     123-314: Missing.

Note: No experimental confirmation available.
Show »
Length:122
Mass (Da):13,462
Checksum:iA884324ECCA5FAF3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471K → R in CAE45887 (PubMed:17974005).Curated
Sequence conflicti77 – 771K → E in BAA92034 (PubMed:14702039).Curated
Sequence conflicti148 – 1481D → Y in BAB14194 (PubMed:14702039).Curated
Sequence conflicti230 – 2301K → E in BAA92034 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti19 – 191A → D.3 Publications
Corresponds to variant rs3748976 [ dbSNP | Ensembl ].
VAR_039204
Natural varianti86 – 861V → I.
Corresponds to variant rs3748980 [ dbSNP | Ensembl ].
VAR_039205
Natural varianti241 – 2411K → R.Combined sources4 Publications
Corresponds to variant rs1713480 [ dbSNP | Ensembl ].
VAR_039206
Natural varianti290 – 2901H → P.
Corresponds to variant rs11645068 [ dbSNP | Ensembl ].
VAR_039207

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei97 – 238142Missing in isoform 2. 1 PublicationVSP_031658Add
BLAST
Alternative sequencei97 – 12226GKPWR…AIPLV → AGQRPPRAPSRGGHRRENCS PILQSR in isoform 3. 1 PublicationVSP_055218Add
BLAST
Alternative sequencei123 – 314192Missing in isoform 3. 1 PublicationVSP_055219Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY553877 mRNA. Translation: AAS64575.1.
AK002013 mRNA. Translation: BAA92034.1.
AK022710 mRNA. Translation: BAB14194.1.
AK298662 mRNA. Translation: BAG60830.1.
BX640805 mRNA. Translation: CAE45887.1.
AC010333 Genomic DNA. No translation available.
AC092324 Genomic DNA. No translation available.
AC109597 Genomic DNA. No translation available.
BC006289 mRNA. Translation: AAH06289.1.
CCDSiCCDS42119.1. [Q9BRF8-2]
CCDS42120.1. [Q9BRF8-1]
RefSeqiNP_001092925.1. NM_001099455.1. [Q9BRF8-2]
NP_060810.2. NM_018340.2. [Q9BRF8-1]
UniGeneiHs.460002.

Genome annotation databases

EnsembliENST00000261660; ENSP00000261660; ENSG00000103381. [Q9BRF8-3]
ENST00000381774; ENSP00000371193; ENSG00000103381. [Q9BRF8-1]
ENST00000433677; ENSP00000411127; ENSG00000103381. [Q9BRF8-2]
GeneIDi55313.
KEGGihsa:55313.
UCSCiuc002dca.5. human. [Q9BRF8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY553877 mRNA. Translation: AAS64575.1.
AK002013 mRNA. Translation: BAA92034.1.
AK022710 mRNA. Translation: BAB14194.1.
AK298662 mRNA. Translation: BAG60830.1.
BX640805 mRNA. Translation: CAE45887.1.
AC010333 Genomic DNA. No translation available.
AC092324 Genomic DNA. No translation available.
AC109597 Genomic DNA. No translation available.
BC006289 mRNA. Translation: AAH06289.1.
CCDSiCCDS42119.1. [Q9BRF8-2]
CCDS42120.1. [Q9BRF8-1]
RefSeqiNP_001092925.1. NM_001099455.1. [Q9BRF8-2]
NP_060810.2. NM_018340.2. [Q9BRF8-1]
UniGeneiHs.460002.

3D structure databases

ProteinModelPortaliQ9BRF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120595. 4 interactions.
IntActiQ9BRF8. 1 interaction.
STRINGi9606.ENSP00000371193.

PTM databases

iPTMnetiQ9BRF8.
PhosphoSiteiQ9BRF8.

Polymorphism and mutation databases

BioMutaiCPPED1.
DMDMi317373448.

Proteomic databases

EPDiQ9BRF8.
MaxQBiQ9BRF8.
PaxDbiQ9BRF8.
PeptideAtlasiQ9BRF8.
PRIDEiQ9BRF8.

Protocols and materials databases

DNASUi55313.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261660; ENSP00000261660; ENSG00000103381. [Q9BRF8-3]
ENST00000381774; ENSP00000371193; ENSG00000103381. [Q9BRF8-1]
ENST00000433677; ENSP00000411127; ENSG00000103381. [Q9BRF8-2]
GeneIDi55313.
KEGGihsa:55313.
UCSCiuc002dca.5. human. [Q9BRF8-1]

Organism-specific databases

CTDi55313.
GeneCardsiCPPED1.
H-InvDBHIX0012827.
HGNCiHGNC:25632. CPPED1.
HPAiHPA040938.
MIMi615603. gene.
neXtProtiNX_Q9BRF8.
PharmGKBiPA164718110.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IP70. Eukaryota.
ENOG410ZI1D. LUCA.
GeneTreeiENSGT00390000008676.
HOGENOMiHOG000291782.
InParanoidiQ9BRF8.
OMAiGCQLGED.
OrthoDBiEOG7QRQW4.
PhylomeDBiQ9BRF8.
TreeFamiTF329406.

Miscellaneous databases

GenomeRNAii55313.
NextBioi35474056.
PROiQ9BRF8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BRF8.
GenevisibleiQ9BRF8. HS.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Transactivating effect of complete S protein of hepatitis B virus and cloning of genes transactivated by complete S protein using suppression subtractive hybridization technique."
    Bai G.-Q., Liu Y., Cheng J., Zhang S.-L., Yue Y.-F., Huang Y.-P., Zhang L.-Y.
    World J. Gastroenterol. 11:3893-3898(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ASP-19 AND ARG-241, INDUCTION.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANTS ASP-19 AND ARG-241.
    Tissue: Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ARG-241.
    Tissue: Small intestine.
  4. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ASP-19 AND ARG-241.
    Tissue: Placenta.
  6. "Downregulation of CPPED1 expression improves glucose metabolism in vitro in adipocytes."
    Vaittinen M., Kaminska D., Kakela P., Eskelinen M., Kolehmainen M., Pihlajamaki J., Uusitupa M., Pulkkinen L.
    Diabetes 62:3747-3750(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "CSTP1, a novel protein phosphatase, blocks cell cycle, promotes cell apoptosis, and suppresses tumor growth of bladder cancer by directly dephosphorylating Akt at Ser473 site."
    Zhuo D.X., Zhang X.W., Jin B., Zhang Z., Xie B.S., Wu C.L., Gong K., Mao Z.B.
    PLoS ONE 8:E65679-E65679(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCPPED_HUMAN
AccessioniPrimary (citable) accession number: Q9BRF8
Secondary accession number(s): B4DQ68
, Q6MZY9, Q9H9M9, Q9NUT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 11, 2011
Last modified: March 16, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.