Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9BRC7

- PLCD4_HUMAN

UniProt

Q9BRC7 - PLCD4_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4

Gene

PLCD4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Binds 3 calcium ions per subunit. Two of the calcium ions are bound to the C2 domain By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei305 – 3051PROSITE-ProRule annotation
Metal bindingi306 – 3061Calcium 1; catalyticBy similarity
Metal bindingi335 – 3351Calcium 1; catalyticBy similarity
Metal bindingi337 – 3371Calcium 1; catalyticBy similarity
Active sitei350 – 3501PROSITE-ProRule annotation
Metal bindingi384 – 3841Calcium 1; catalyticBy similarity
Binding sitei433 – 4331SubstrateBy similarity
Binding sitei435 – 4351SubstrateBy similarity
Binding sitei522 – 5221SubstrateBy similarity
Binding sitei549 – 5491SubstrateBy similarity
Metal bindingi650 – 6501Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi652 – 6521Calcium 2By similarity
Metal bindingi676 – 6761Calcium 2By similarity
Metal bindingi705 – 7051Calcium 3By similarity
Metal bindingi706 – 7061Calcium 3; via carbonyl oxygenBy similarity
Metal bindingi707 – 7071Calcium 3By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi147 – 158121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi183 – 194122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. acrosome reaction Source: Ensembl
  2. intracellular signal transduction Source: InterPro
  3. lipid catabolic process Source: UniProtKB-KW
  4. phosphatidylinositol metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (EC:3.1.4.11)
Short name:
hPLCD4
Alternative name(s):
Phosphoinositide phospholipase C-delta-4
Phospholipase C-delta-4
Short name:
PLC-delta-4
Gene namesi
Name:PLCD4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:9062. PLCD4.

Subcellular locationi

Membrane By similarity; Peripheral membrane protein By similarity. Nucleus By similarity. Cytoplasm 1 Publication. Endoplasmic reticulum By similarity
Note: Localizes primarily to intracellular membranes mostly to the endoplasmic reticulum.

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33390.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7627621-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4PRO_0000306824Add
BLAST

Proteomic databases

PaxDbiQ9BRC7.
PRIDEiQ9BRC7.

PTM databases

PhosphoSiteiQ9BRC7.

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle and kidney tissues, and at moderate level in intestinal tissue. Expressed in corneal epithelial cells.2 Publications

Gene expression databases

BgeeiQ9BRC7.
CleanExiHS_PLCD4.
ExpressionAtlasiQ9BRC7. baseline and differential.
GenevestigatoriQ9BRC7.

Organism-specific databases

HPAiCAB009914.

Interactioni

Subunit structurei

Interacts with GRIP1.By similarity

Protein-protein interaction databases

BioGridi124273. 3 interactions.
IntActiQ9BRC7. 2 interactions.
MINTiMINT-1482361.
STRINGi9606.ENSP00000388631.

Structurei

3D structure databases

ProteinModelPortaliQ9BRC7.
SMRiQ9BRC7. Positions 9-123, 149-755.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 124109PHPROSITE-ProRule annotationAdd
BLAST
Domaini134 – 16936EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini170 – 20536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini203 – 23735EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini290 – 435146PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini493 – 609117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini614 – 719106C2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni26 – 5328Substrate bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi731 – 7344PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi440 – 47334Glu-richAdd
BLAST
Compositional biasi479 – 4868Poly-Lys

Domaini

The PDZ-binding motif mediates the interaction with GRIP1.
The C2 domain mediates pre-localization to the membrane prior to Ca2+ import and non-selective Ca2+-mediated targeting to various cellular membranes.
The PH domain is not a critical determinant of the membrane localization.

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG149692.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
InParanoidiQ9BRC7.
KOiK05857.
OrthoDBiEOG7V49XT.
PhylomeDBiQ9BRC7.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028387. PLC-delta4.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF31. PTHR10336:SF31. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 3 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9BRC7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLQDQLT TDQDLLLMQE GMPMRKVRSK SWKKLRYFRL QNDGMTVWHA
60 70 80 90 100
RQARGSAKPS FSISDVETIR NGHDSELLRS LAEELPLEQG FTIVFHGRRS
110 120 130 140 150
NLDLMANSVE EAQIWMRGLQ LLVDLVTSMD HQERLDQWLS DWFQRGDKNQ
160 170 180 190 200
DGKMSFQEVQ RLLHLMNVEM DQEYAFSLFQ AADTSQSGTL EGEEFVQFYK
210 220 230 240 250
ALTKRAEVQE LFESFSADGQ KLTLLEFLDF LQEEQKERDC TSELALELID
260 270 280 290 300
RYEPSDSGKL RHVLSMDGFL SYLCSKDGDI FNPACLPIYQ DMTQPLNHYF
310 320 330 340 350
ICSSHNTYLV GDQLCGQSSV EGYIRALKRG CRCVEVDVWD GPSGEPVVYH
360 370 380 390 400
GHTLTSRILF KDVVATVAQY AFQTSDYPVI LSLETHCSWE QQQTMARHLT
410 420 430 440 450
EILGEQLLST TLDGVLPTQL PSPEELRRKI LVKGKKLTLE EDLEYEEEEA
460 470 480 490 500
EPELEESELA LESQFETEPE PQEQNLQNKD KKKKSKPILC PALSSLVIYL
510 520 530 540 550
KSVSFRSFTH SKEHYHFYEI SSFSETKAKR LIKEAGNEFV QHNTWQLSRV
560 570 580 590 600
YPSGLRTDSS NYNPQELWNA GCQMVAMNMQ TAGLEMDICD GHFRQNGGCG
610 620 630 640 650
YVLKPDFLRD IQSSFHPEKP ISPFKAQTLL IQVISGQQLP KVDKTKEGSI
660 670 680 690 700
VDPLVKVQIF GVRLDTARQE TNYVENNGFN PYWGQTLCFR VLVPELAMLR
710 720 730 740 750
FVVMDYDWKS RNDFIGQYTL PWTCMQQGYR HIHLLSKDGI SLRPASIFVY
760
ICIQEGLEGD ES

Note: Acceptor splice site between exons 4 and 5 is non-canonical but conserved through species for that particular gene.

Length:762
Mass (Da):87,585
Last modified:June 1, 2001 - v1
Checksum:i5444BE5CE2AEA3EF
GO
Isoform 2 (identifier: Q9BRC7-2) [UniParc]FASTAAdd to Basket

Also known as: PLCD4b

The sequence of this isoform differs from the canonical sequence as follows:
     259-272: KLRHVLSMDGFLSY → ASEEDPGEGEEVNT
     273-762: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:272
Mass (Da):31,448
Checksum:iC3A83C9C8E411D88
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981R → H in BAD96923. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei259 – 27214KLRHV…GFLSY → ASEEDPGEGEEVNT in isoform 2. 1 PublicationVSP_028501Add
BLAST
Alternative sequencei273 – 762490Missing in isoform 2. 1 PublicationVSP_028502Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY512961 mRNA. Translation: AAS82574.1.
AK223203 mRNA. Translation: BAD96923.1.
BC006355 mRNA. Translation: AAH06355.1.
CCDSiCCDS46516.1. [Q9BRC7-1]
RefSeqiNP_116115.1. NM_032726.3. [Q9BRC7-1]
UniGeneiHs.632528.

Genome annotation databases

EnsembliENST00000417849; ENSP00000396942; ENSG00000115556. [Q9BRC7-1]
ENST00000450993; ENSP00000388631; ENSG00000115556. [Q9BRC7-1]
GeneIDi84812.
KEGGihsa:84812.
UCSCiuc021vwx.1. human. [Q9BRC7-1]

Polymorphism databases

DMDMi74732863.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY512961 mRNA. Translation: AAS82574.1 .
AK223203 mRNA. Translation: BAD96923.1 .
BC006355 mRNA. Translation: AAH06355.1 .
CCDSi CCDS46516.1. [Q9BRC7-1 ]
RefSeqi NP_116115.1. NM_032726.3. [Q9BRC7-1 ]
UniGenei Hs.632528.

3D structure databases

ProteinModelPortali Q9BRC7.
SMRi Q9BRC7. Positions 9-123, 149-755.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 124273. 3 interactions.
IntActi Q9BRC7. 2 interactions.
MINTi MINT-1482361.
STRINGi 9606.ENSP00000388631.

PTM databases

PhosphoSitei Q9BRC7.

Polymorphism databases

DMDMi 74732863.

Proteomic databases

PaxDbi Q9BRC7.
PRIDEi Q9BRC7.

Protocols and materials databases

DNASUi 84812.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000417849 ; ENSP00000396942 ; ENSG00000115556 . [Q9BRC7-1 ]
ENST00000450993 ; ENSP00000388631 ; ENSG00000115556 . [Q9BRC7-1 ]
GeneIDi 84812.
KEGGi hsa:84812.
UCSCi uc021vwx.1. human. [Q9BRC7-1 ]

Organism-specific databases

CTDi 84812.
GeneCardsi GC02P219473.
H-InvDB HIX0002832.
HIX0030357.
HGNCi HGNC:9062. PLCD4.
HPAi CAB009914.
MIMi 605939. gene.
neXtProti NX_Q9BRC7.
PharmGKBi PA33390.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG149692.
GeneTreei ENSGT00760000118936.
HOGENOMi HOG000006871.
HOVERGENi HBG053610.
InParanoidi Q9BRC7.
KOi K05857.
OrthoDBi EOG7V49XT.
PhylomeDBi Q9BRC7.
TreeFami TF313216.

Miscellaneous databases

ChiTaRSi PLCD4. human.
GeneWikii PLCD4.
GenomeRNAii 84812.
NextBioi 74997.
PROi Q9BRC7.
SOURCEi Search...

Gene expression databases

Bgeei Q9BRC7.
CleanExi HS_PLCD4.
ExpressionAtlasi Q9BRC7. baseline and differential.
Genevestigatori Q9BRC7.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR028387. PLC-delta4.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF31. PTHR10336:SF31. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00054. EFh. 3 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Phospholipase C delta-4 overexpression upregulates ErbB1/2 expression, Erk signaling pathway, and proliferation in MCF-7 cells."
    Leung D.W., Tompkins C., Brewer J., Ball A., Coon M., Morris V., Waggoner D., Singer J.W.
    Mol. Cancer 3:15-15(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney proximal tubule.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. "Assignment of the human PLC delta4 gene (PLCD4) to human chromosome band 2q35 by fluorescence in situ hybridization."
    Kim H., Suh P.-G., Ryu S.H., Park S.H.
    Cytogenet. Cell Genet. 87:254-255(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "Expression of phospholipases A2 and C in human corneal epithelial cells."
    Landreville S., Coulombe S., Carrier P., Gelb M.H., Guerin S.L., Salesse C.
    Invest. Ophthalmol. Vis. Sci. 45:3997-4003(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLCD4_HUMAN
AccessioniPrimary (citable) accession number: Q9BRC7
Secondary accession number(s): Q53FS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3