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Q9BQQ3

- GORS1_HUMAN

UniProt

Q9BQQ3 - GORS1_HUMAN

Protein

Golgi reassembly-stacking protein 1

Gene

GORASP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.

    GO - Biological processi

    1. Golgi organization Source: UniProtKB
    2. mitotic cell cycle Source: Reactome
    3. negative regulation of dendrite morphogenesis Source: UniProtKB
    4. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_1100. Golgi Cisternae Pericentriolar Stack Reorganization.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Golgi reassembly-stacking protein 1
    Alternative name(s):
    Golgi peripheral membrane protein p65
    Golgi phosphoprotein 5
    Short name:
    GOLPH5
    Golgi reassembly-stacking protein of 65 kDa
    Short name:
    GRASP65
    Gene namesi
    Name:GORASP1
    Synonyms:GOLPH5, GRASP65
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:16769. GORASP1.

    Subcellular locationi

    Golgi apparatuscis-Golgi network membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
    Note: Undergoes rapid exchange with the cytosol.

    GO - Cellular componenti

    1. Golgi membrane Source: Reactome

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28814.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedCurated
    Chaini2 – 440439Golgi reassembly-stacking protein 1PRO_0000087570Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineCurated
    Modified residuei216 – 2161Phosphothreonine3 Publications
    Modified residuei373 – 3731PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking.5 Publications
    Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Proteomic databases

    MaxQBiQ9BQQ3.
    PaxDbiQ9BQQ3.
    PRIDEiQ9BQQ3.

    PTM databases

    PhosphoSiteiQ9BQQ3.

    Miscellaneous databases

    PMAP-CutDBQ9BQQ3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9BQQ3.
    BgeeiQ9BQQ3.
    CleanExiHS_GORASP1.
    GenevestigatoriQ9BQQ3.

    Organism-specific databases

    HPAiHPA056283.

    Interactioni

    Subunit structurei

    Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. The complex binds to the vesicle docking protein p115. Interacts with TMED2 and TMED3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi122232. 13 interactions.
    IntActiQ9BQQ3. 4 interactions.
    MINTiMINT-1185294.
    STRINGi9606.ENSP00000313869.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BQQ3.
    SMRiQ9BQQ3. Positions 13-211.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 7571PDZAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni190 – 20213Essential for the interaction with GM130By similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi203 – 29896Pro-richAdd
    BLAST
    Compositional biasi295 – 2984Poly-Pro
    Compositional biasi348 – 3525Poly-Ser

    Sequence similaritiesi

    Belongs to the GORASP family.Curated
    Contains 1 PDZ (DHR) domain.Curated

    Phylogenomic databases

    eggNOGiCOG5233.
    HOGENOMiHOG000054196.
    HOVERGENiHBG051826.
    InParanoidiQ9BQQ3.
    OMAiSGPEDVC.
    OrthoDBiEOG7BS4B1.
    PhylomeDBiQ9BQQ3.

    Family and domain databases

    Gene3Di2.30.42.10. 1 hit.
    InterProiIPR024958. GRASP55/65_PDZ.
    IPR007583. GRASP55_65.
    IPR001478. PDZ.
    [Graphical view]
    PANTHERiPTHR12893. PTHR12893. 1 hit.
    PfamiPF04495. GRASP55_65. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 2 hits.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9BQQ3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGLGVSAEQP AGGAEGFHLH GVQENSPAQQ AGLEPYFDFI ITIGHSRLNK    50
    ENDTLKALLK ANVEKPVKLE VFNMKTMRVR EVEVVPSNMW GGQGLLGASV 100
    RFCSFRRASE QVWHVLDVEP SSPAALAGLR PYTDYVVGSD QILQESEDFF 150
    TLIESHEGKP LKLMVYNSKS DSCREVTVTP NAAWGGEGSL GCGIGYGYLH 200
    RIPTQPPSYH KKPPGTPPPS ALPLGAPPPD ALPPGPTPED SPSLETGSRQ 250
    SDYMEALLQA PGSSMEDPLP GPGSPSHSAP DPDGLPHFME TPLQPPPPVQ 300
    RVMDPGFLDV SGISLLDNSN ASVWPSLPSS TELTTTAVST SGPEDICSSS 350
    SSHERGGEAT WSGSEFEVSF LDSPGAQAQA DHLPQLTLPD SLTSAASPED 400
    GLSAELLEAQ AEEEPASTEG LDTGTEAEGL DSQAQISTTE 440
    Length:440
    Mass (Da):46,482
    Last modified:January 23, 2007 - v3
    Checksum:i1187C0356E16CE00
    GO
    Isoform 2 (identifier: Q9BQQ3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         176-440: VTVTPNAAWG...DSQAQISTTE → SGMWHWLWVS...FCSTTWCPTT

    Note: No experimental confirmation available.

    Show »
    Length:215
    Mass (Da):23,906
    Checksum:i0EC77420BA924801
    GO
    Isoform 3 (identifier: Q9BQQ3-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-73: Missing.
         306-319: GFLDVSGISLLDNS → AMPVCGPACPLPQN
         320-440: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:246
    Mass (Da):26,237
    Checksum:i9EC9AC172F80403D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti271 – 2711G → E in BAC03598. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti425 – 4251T → M.
    Corresponds to variant rs1109643 [ dbSNP | Ensembl ].
    VAR_051016

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7373Missing in isoform 3. 1 PublicationVSP_011306Add
    BLAST
    Alternative sequencei176 – 440265VTVTP…ISTTE → SGMWHWLWVSTPDPNSAPQL PQEATWHPTTFCSTTWCPTT in isoform 2. 1 PublicationVSP_011307Add
    BLAST
    Alternative sequencei306 – 31914GFLDV…LLDNS → AMPVCGPACPLPQN in isoform 3. 1 PublicationVSP_011308Add
    BLAST
    Alternative sequencei320 – 440121Missing in isoform 3. 1 PublicationVSP_011309Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ409349 mRNA. Translation: CAC35160.1.
    AK091168 mRNA. Translation: BAC03598.1.
    AK124755 mRNA. Translation: BAG54090.1.
    CH471055 Genomic DNA. Translation: EAW64554.1.
    BC008928 mRNA. Translation: AAH08928.1.
    BC075854 mRNA. Translation: AAH75854.1.
    BC103826 mRNA. Translation: AAI03827.1.
    BC103827 mRNA. Translation: AAI03828.1.
    CCDSiCCDS2681.1. [Q9BQQ3-1]
    RefSeqiNP_001265718.1. NM_001278789.1.
    NP_001265719.1. NM_001278790.1.
    NP_114105.1. NM_031899.3. [Q9BQQ3-1]
    UniGeneiHs.721946.

    Genome annotation databases

    EnsembliENST00000319283; ENSP00000313869; ENSG00000114745. [Q9BQQ3-1]
    ENST00000431601; ENSP00000403552; ENSG00000114745. [Q9BQQ3-3]
    ENST00000452389; ENSP00000403167; ENSG00000114745. [Q9BQQ3-2]
    GeneIDi64689.
    KEGGihsa:64689.
    UCSCiuc003ciw.1. human. [Q9BQQ3-1]

    Polymorphism databases

    DMDMi51316077.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ409349 mRNA. Translation: CAC35160.1 .
    AK091168 mRNA. Translation: BAC03598.1 .
    AK124755 mRNA. Translation: BAG54090.1 .
    CH471055 Genomic DNA. Translation: EAW64554.1 .
    BC008928 mRNA. Translation: AAH08928.1 .
    BC075854 mRNA. Translation: AAH75854.1 .
    BC103826 mRNA. Translation: AAI03827.1 .
    BC103827 mRNA. Translation: AAI03828.1 .
    CCDSi CCDS2681.1. [Q9BQQ3-1 ]
    RefSeqi NP_001265718.1. NM_001278789.1.
    NP_001265719.1. NM_001278790.1.
    NP_114105.1. NM_031899.3. [Q9BQQ3-1 ]
    UniGenei Hs.721946.

    3D structure databases

    ProteinModelPortali Q9BQQ3.
    SMRi Q9BQQ3. Positions 13-211.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122232. 13 interactions.
    IntActi Q9BQQ3. 4 interactions.
    MINTi MINT-1185294.
    STRINGi 9606.ENSP00000313869.

    PTM databases

    PhosphoSitei Q9BQQ3.

    Polymorphism databases

    DMDMi 51316077.

    Proteomic databases

    MaxQBi Q9BQQ3.
    PaxDbi Q9BQQ3.
    PRIDEi Q9BQQ3.

    Protocols and materials databases

    DNASUi 64689.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000319283 ; ENSP00000313869 ; ENSG00000114745 . [Q9BQQ3-1 ]
    ENST00000431601 ; ENSP00000403552 ; ENSG00000114745 . [Q9BQQ3-3 ]
    ENST00000452389 ; ENSP00000403167 ; ENSG00000114745 . [Q9BQQ3-2 ]
    GeneIDi 64689.
    KEGGi hsa:64689.
    UCSCi uc003ciw.1. human. [Q9BQQ3-1 ]

    Organism-specific databases

    CTDi 64689.
    GeneCardsi GC03M039113.
    HGNCi HGNC:16769. GORASP1.
    HPAi HPA056283.
    MIMi 606867. gene.
    neXtProti NX_Q9BQQ3.
    PharmGKBi PA28814.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5233.
    HOGENOMi HOG000054196.
    HOVERGENi HBG051826.
    InParanoidi Q9BQQ3.
    OMAi SGPEDVC.
    OrthoDBi EOG7BS4B1.
    PhylomeDBi Q9BQQ3.

    Enzyme and pathway databases

    Reactomei REACT_1100. Golgi Cisternae Pericentriolar Stack Reorganization.

    Miscellaneous databases

    GeneWikii GORASP1.
    GenomeRNAii 64689.
    NextBioi 66611.
    PMAP-CutDB Q9BQQ3.
    PROi Q9BQQ3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BQQ3.
    Bgeei Q9BQQ3.
    CleanExi HS_GORASP1.
    Genevestigatori Q9BQQ3.

    Family and domain databases

    Gene3Di 2.30.42.10. 1 hit.
    InterProi IPR024958. GRASP55/65_PDZ.
    IPR007583. GRASP55_65.
    IPR001478. PDZ.
    [Graphical view ]
    PANTHERi PTHR12893. PTHR12893. 1 hit.
    Pfami PF04495. GRASP55_65. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Refined physical mapping and genomic structure of a 4-Mb region (AP-20) on human chromosome 3p22-p21.33 implicated in lung and kidney cancerogenesis."
      Protopopov A., Kashuba V., Kvasha S., Klein G., Zabarovsky E.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Lung.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Tissue: Brain and Tongue.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Eye and Placenta.
    5. "Maintenance of Golgi structure and function depends on the integrity of ER export."
      Ward T.H., Polishchuk R.S., Caplan S., Hirschberg K., Lippincott-Schwartz J.
      J. Cell Biol. 155:557-570(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION IN THE CYTOSOL.
    6. "A direct role for GRASP65 as a mitotically regulated Golgi stacking factor."
      Wang Y., Seemann J., Pypaert M., Shorter J., Warren G.
      EMBO J. 22:3279-3290(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, OLIGOMERIZATION.
    7. "Peripheral Golgi protein GRASP65 is a target of mitotic polo-like kinase (Plk) and Cdc2."
      Lin C.-Y., Madsen M.L., Yarm F.R., Jang Y.-J., Liu X., Erikson R.L.
      Proc. Natl. Acad. Sci. U.S.A. 97:12589-12594(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiGORS1_HUMAN
    AccessioniPrimary (citable) accession number: Q9BQQ3
    Secondary accession number(s): B3KWC8
    , Q3SYG7, Q8N272, Q96H42
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 108 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3