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Protein

Golgi reassembly-stacking protein 1

Gene

GORASP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (PubMed:16489344).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 1
Alternative name(s):
Golgi peripheral membrane protein p65
Golgi phosphoprotein 5
Short name:
GOLPH5
Golgi reassembly-stacking protein of 65 kDa
Short name:
GRASP65
Gene namesi
Name:GORASP1
Synonyms:GOLPH5, GRASP65
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:16769. GORASP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi64689.
OpenTargetsiENSG00000114745.
PharmGKBiPA28814.

Polymorphism and mutation databases

BioMutaiGORASP1.
DMDMi51316077.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00000875702 – 440Golgi reassembly-stacking protein 1Add BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineCurated1
Modified residuei216PhosphothreonineCombined sources1
Modified residuei362PhosphoserineBy similarity1
Modified residuei364PhosphoserineBy similarity1
Modified residuei373PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking.2 Publications
Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ9BQQ3.
MaxQBiQ9BQQ3.
PaxDbiQ9BQQ3.
PeptideAtlasiQ9BQQ3.
PRIDEiQ9BQQ3.

PTM databases

iPTMnetiQ9BQQ3.
PhosphoSitePlusiQ9BQQ3.

Miscellaneous databases

PMAP-CutDBQ9BQQ3.

Expressioni

Gene expression databases

BgeeiENSG00000114745.
CleanExiHS_GORASP1.
ExpressionAtlasiQ9BQQ3. baseline and differential.
GenevisibleiQ9BQQ3. HS.

Organism-specific databases

HPAiHPA056283.

Interactioni

Subunit structurei

Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae (By similarity). Interacts with the C-terminus of GOLGA2/GM130 under both mitotic and non-mitotic conditions (PubMed:16489344). The interaction is critical for the correct targeting of both proteins to the cis-Golgi (By similarity). The complex binds to the vesicle docking protein p115/USO1. Interacts with TMED2 and TMED3 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AGPSO001165EBI-2561458,EBI-2838732
LONRF1Q17RB85EBI-2561458,EBI-2341787
SCRN1Q127653EBI-2561458,EBI-2690712

Protein-protein interaction databases

BioGridi122232. 53 interactors.
IntActiQ9BQQ3. 10 interactors.
MINTiMINT-1185294.
STRINGi9606.ENSP00000313869.

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 22Combined sources9
Helixi27 – 30Combined sources4
Turni35 – 37Combined sources3
Beta strandi38 – 43Combined sources6
Beta strandi50 – 53Combined sources4
Helixi54 – 61Combined sources8
Turni62 – 64Combined sources3
Beta strandi67 – 73Combined sources7
Turni74 – 77Combined sources4
Beta strandi78 – 84Combined sources7
Beta strandi90 – 96Combined sources7
Beta strandi98 – 104Combined sources7
Helixi108 – 110Combined sources3
Beta strandi113 – 118Combined sources6
Helixi123 – 127Combined sources5
Turni131 – 133Combined sources3
Beta strandi134 – 141Combined sources8
Helixi149 – 155Combined sources7
Beta strandi161 – 167Combined sources7
Turni168 – 171Combined sources4
Beta strandi172 – 178Combined sources7
Beta strandi184 – 190Combined sources7
Beta strandi192 – 195Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4REYX-ray1.96A2-210[»]
ProteinModelPortaliQ9BQQ3.
SMRiQ9BQQ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 75PDZAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 202Essential for interaction with GOLGA2/GM130By similarityAdd BLAST13

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi203 – 298Pro-richAdd BLAST96
Compositional biasi295 – 298Poly-Pro4
Compositional biasi348 – 352Poly-Ser5

Sequence similaritiesi

Belongs to the GORASP family.Curated
Contains 1 PDZ (DHR) domain.Curated

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
GeneTreeiENSGT00390000008686.
HOGENOMiHOG000054196.
HOVERGENiHBG051826.
InParanoidiQ9BQQ3.
OMAiSGPEDVC.
OrthoDBiEOG091G0E90.
PhylomeDBiQ9BQQ3.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BQQ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLGVSAEQP AGGAEGFHLH GVQENSPAQQ AGLEPYFDFI ITIGHSRLNK
60 70 80 90 100
ENDTLKALLK ANVEKPVKLE VFNMKTMRVR EVEVVPSNMW GGQGLLGASV
110 120 130 140 150
RFCSFRRASE QVWHVLDVEP SSPAALAGLR PYTDYVVGSD QILQESEDFF
160 170 180 190 200
TLIESHEGKP LKLMVYNSKS DSCREVTVTP NAAWGGEGSL GCGIGYGYLH
210 220 230 240 250
RIPTQPPSYH KKPPGTPPPS ALPLGAPPPD ALPPGPTPED SPSLETGSRQ
260 270 280 290 300
SDYMEALLQA PGSSMEDPLP GPGSPSHSAP DPDGLPHFME TPLQPPPPVQ
310 320 330 340 350
RVMDPGFLDV SGISLLDNSN ASVWPSLPSS TELTTTAVST SGPEDICSSS
360 370 380 390 400
SSHERGGEAT WSGSEFEVSF LDSPGAQAQA DHLPQLTLPD SLTSAASPED
410 420 430 440
GLSAELLEAQ AEEEPASTEG LDTGTEAEGL DSQAQISTTE
Length:440
Mass (Da):46,482
Last modified:January 23, 2007 - v3
Checksum:i1187C0356E16CE00
GO
Isoform 2 (identifier: Q9BQQ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-440: VTVTPNAAWG...DSQAQISTTE → SGMWHWLWVS...FCSTTWCPTT

Note: No experimental confirmation available.
Show »
Length:215
Mass (Da):23,906
Checksum:i0EC77420BA924801
GO
Isoform 3 (identifier: Q9BQQ3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.
     306-319: GFLDVSGISLLDNS → AMPVCGPACPLPQN
     320-440: Missing.

Note: No experimental confirmation available.
Show »
Length:246
Mass (Da):26,237
Checksum:i9EC9AC172F80403D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271G → E in BAC03598 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051016425T → M.Corresponds to variant rs1109643dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0113061 – 73Missing in isoform 3. 1 PublicationAdd BLAST73
Alternative sequenceiVSP_011307176 – 440VTVTP…ISTTE → SGMWHWLWVSTPDPNSAPQL PQEATWHPTTFCSTTWCPTT in isoform 2. 1 PublicationAdd BLAST265
Alternative sequenceiVSP_011308306 – 319GFLDV…LLDNS → AMPVCGPACPLPQN in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_011309320 – 440Missing in isoform 3. 1 PublicationAdd BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ409349 mRNA. Translation: CAC35160.1.
AK091168 mRNA. Translation: BAC03598.1.
AK124755 mRNA. Translation: BAG54090.1.
CH471055 Genomic DNA. Translation: EAW64554.1.
BC008928 mRNA. Translation: AAH08928.1.
BC075854 mRNA. Translation: AAH75854.1.
BC103826 mRNA. Translation: AAI03827.1.
BC103827 mRNA. Translation: AAI03828.1.
CCDSiCCDS2681.1. [Q9BQQ3-1]
RefSeqiNP_001265718.1. NM_001278789.1.
NP_001265719.1. NM_001278790.1.
NP_114105.1. NM_031899.3. [Q9BQQ3-1]
UniGeneiHs.721946.

Genome annotation databases

EnsembliENST00000319283; ENSP00000313869; ENSG00000114745. [Q9BQQ3-1]
ENST00000431601; ENSP00000403552; ENSG00000114745. [Q9BQQ3-3]
ENST00000452389; ENSP00000403167; ENSG00000114745. [Q9BQQ3-2]
GeneIDi64689.
KEGGihsa:64689.
UCSCiuc003ciw.3. human. [Q9BQQ3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ409349 mRNA. Translation: CAC35160.1.
AK091168 mRNA. Translation: BAC03598.1.
AK124755 mRNA. Translation: BAG54090.1.
CH471055 Genomic DNA. Translation: EAW64554.1.
BC008928 mRNA. Translation: AAH08928.1.
BC075854 mRNA. Translation: AAH75854.1.
BC103826 mRNA. Translation: AAI03827.1.
BC103827 mRNA. Translation: AAI03828.1.
CCDSiCCDS2681.1. [Q9BQQ3-1]
RefSeqiNP_001265718.1. NM_001278789.1.
NP_001265719.1. NM_001278790.1.
NP_114105.1. NM_031899.3. [Q9BQQ3-1]
UniGeneiHs.721946.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4REYX-ray1.96A2-210[»]
ProteinModelPortaliQ9BQQ3.
SMRiQ9BQQ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122232. 53 interactors.
IntActiQ9BQQ3. 10 interactors.
MINTiMINT-1185294.
STRINGi9606.ENSP00000313869.

PTM databases

iPTMnetiQ9BQQ3.
PhosphoSitePlusiQ9BQQ3.

Polymorphism and mutation databases

BioMutaiGORASP1.
DMDMi51316077.

Proteomic databases

EPDiQ9BQQ3.
MaxQBiQ9BQQ3.
PaxDbiQ9BQQ3.
PeptideAtlasiQ9BQQ3.
PRIDEiQ9BQQ3.

Protocols and materials databases

DNASUi64689.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319283; ENSP00000313869; ENSG00000114745. [Q9BQQ3-1]
ENST00000431601; ENSP00000403552; ENSG00000114745. [Q9BQQ3-3]
ENST00000452389; ENSP00000403167; ENSG00000114745. [Q9BQQ3-2]
GeneIDi64689.
KEGGihsa:64689.
UCSCiuc003ciw.3. human. [Q9BQQ3-1]

Organism-specific databases

CTDi64689.
DisGeNETi64689.
GeneCardsiGORASP1.
HGNCiHGNC:16769. GORASP1.
HPAiHPA056283.
MIMi606867. gene.
neXtProtiNX_Q9BQQ3.
OpenTargetsiENSG00000114745.
PharmGKBiPA28814.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3834. Eukaryota.
COG5233. LUCA.
GeneTreeiENSGT00390000008686.
HOGENOMiHOG000054196.
HOVERGENiHBG051826.
InParanoidiQ9BQQ3.
OMAiSGPEDVC.
OrthoDBiEOG091G0E90.
PhylomeDBiQ9BQQ3.

Enzyme and pathway databases

ReactomeiR-HSA-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-HSA-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-HSA-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

ChiTaRSiGORASP1. human.
GeneWikiiGORASP1.
GenomeRNAii64689.
PMAP-CutDBQ9BQQ3.
PROiQ9BQQ3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114745.
CleanExiHS_GORASP1.
ExpressionAtlasiQ9BQQ3. baseline and differential.
GenevisibleiQ9BQQ3. HS.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR024958. GRASP55/65_PDZ.
IPR007583. GRASP55_65.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR12893. PTHR12893. 1 hit.
PfamiPF04495. GRASP55_65. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGORS1_HUMAN
AccessioniPrimary (citable) accession number: Q9BQQ3
Secondary accession number(s): B3KWC8
, Q3SYG7, Q8N272, Q96H42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.