Q9BQQ3 (GORS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi reassembly-stacking protein 1

Alternative name(s):
Golgi peripheral membrane protein p65
Golgi phosphoprotein 5
Short name=GOLPH5
Golgi reassembly-stacking protein of 65 kDa
Short name=GRASP65

Gene names

Name:	GORASP1
Synonyms:	GOLPH5, GRASP65

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes.
Subunit structure	Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. The complex binds to the vesicle docking protein p115. Interacts with TMED2 and TMED3 By similarity. Ref.6
Subcellular location	Golgi apparatus › cis-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Note: Undergoes rapid exchange with the cytosol. Ref.5
Post-translational modification	Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking. Ref.6 Ref.7 Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis By similarity.
Sequence similarities	Belongs to the GORASP family. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitotic prophase Traceable author statement. Source: Reactome protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome cytosol Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BQQ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BQQ3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     176-440: VTVTPNAAWG...DSQAQISTTE → SGMWHWLWVS...FCSTTWCPTT

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9BQQ3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.
     306-319: GFLDVSGISLLDNS → AMPVCGPACPLPQN
     320-440: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Golgi reassembly-stacking protein 1		PRO_0000087570
Regions
	Domain	5 – 75	71	PDZ
	Region	190 – 202	13	Essential for the interaction with GM130 By similarity
	Compositional bias	203 – 298	96	Pro-rich
	Compositional bias	295 – 298	4	Poly-Pro
	Compositional bias	348 – 352	5	Poly-Ser
Amino acid modifications
	Modified residue	216	1	Phosphothreonine Ref.8 Ref.9 Ref.10
	Lipidation	2	1	N-myristoyl glycine Probable
Natural variations
	Alternative sequence	1 – 73	73	Missing in isoform 3.		VSP_011306
	Alternative sequence	176 – 440	265	VTVTP…ISTTE → SGMWHWLWVSTPDPNSAPQL PQEATWHPTTFCSTTWCPTT in isoform 2.		VSP_011307
	Alternative sequence	306 – 319	14	GFLDV…LLDNS → AMPVCGPACPLPQN in isoform 3.		VSP_011308
	Alternative sequence	320 – 440	121	Missing in isoform 3.		VSP_011309
	Natural variant	425	1	T → M. Corresponds to variant rs1109643 [ dbSNP | Ensembl ].		VAR_051016
Experimental info
	Sequence conflict	271	1	G → E in BAC03598. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1187C0356E16CE00	FASTA	440	46,482
	10 20 30 40 50 60 MGLGVSAEQP AGGAEGFHLH GVQENSPAQQ AGLEPYFDFI ITIGHSRLNK ENDTLKALLK 70 80 90 100 110 120 ANVEKPVKLE VFNMKTMRVR EVEVVPSNMW GGQGLLGASV RFCSFRRASE QVWHVLDVEP 130 140 150 160 170 180 SSPAALAGLR PYTDYVVGSD QILQESEDFF TLIESHEGKP LKLMVYNSKS DSCREVTVTP 190 200 210 220 230 240 NAAWGGEGSL GCGIGYGYLH RIPTQPPSYH KKPPGTPPPS ALPLGAPPPD ALPPGPTPED 250 260 270 280 290 300 SPSLETGSRQ SDYMEALLQA PGSSMEDPLP GPGSPSHSAP DPDGLPHFME TPLQPPPPVQ 310 320 330 340 350 360 RVMDPGFLDV SGISLLDNSN ASVWPSLPSS TELTTTAVST SGPEDICSSS SSHERGGEAT 370 380 390 400 410 420 WSGSEFEVSF LDSPGAQAQA DHLPQLTLPD SLTSAASPED GLSAELLEAQ AEEEPASTEG 430 440 LDTGTEAEGL DSQAQISTTE
	Isoform 2 [UniParc]. Checksum: 0EC77420BA924801	FASTA	215	23,906
	10 20 30 40 50 60 MGLGVSAEQP AGGAEGFHLH GVQENSPAQQ AGLEPYFDFI ITIGHSRLNK ENDTLKALLK 70 80 90 100 110 120 ANVEKPVKLE VFNMKTMRVR EVEVVPSNMW GGQGLLGASV RFCSFRRASE QVWHVLDVEP 130 140 150 160 170 180 SSPAALAGLR PYTDYVVGSD QILQESEDFF TLIESHEGKP LKLMVYNSKS DSCRESGMWH 190 200 210 WLWVSTPDPN SAPQLPQEAT WHPTTFCSTT WCPTT
	Isoform 3 [UniParc]. Checksum: 9EC9AC172F80403D	FASTA	246	26,237
	10 20 30 40 50 60 MKTMRVREVE VVPSNMWGGQ GLLGASVRFC SFRRASEQVW HVLDVEPSSP AALAGLRPYT 70 80 90 100 110 120 DYVVGSDQIL QESEDFFTLI ESHEGKPLKL MVYNSKSDSC REVTVTPNAA WGGEGSLGCG 130 140 150 160 170 180 IGYGYLHRIP TQPPSYHKKP PGTPPPSALP LGAPPPDALP PGPTPEDSPS LETGSRQSDY 190 200 210 220 230 240 MEALLQAPGS SMEDPLPGPG SPSHSAPDPD GLPHFMETPL QPPPPVQRVM DPAMPVCGPA CPLPQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Refined physical mapping and genomic structure of a 4-Mb region (AP-20) on human chromosome 3p22-p21.33 implicated in lung and kidney cancerogenesis." Protopopov A., Kashuba V., Kvasha S., Klein G., Zabarovsky E. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Lung.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain and Tongue.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Placenta.
[5]	"Maintenance of Golgi structure and function depends on the integrity of ER export." Ward T.H., Polishchuk R.S., Caplan S., Hirschberg K., Lippincott-Schwartz J. J. Cell Biol. 155:557-570(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION IN THE CYTOSOL.
[6]	"A direct role for GRASP65 as a mitotically regulated Golgi stacking factor." Wang Y., Seemann J., Pypaert M., Shorter J., Warren G. EMBO J. 22:3279-3290(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, OLIGOMERIZATION.
[7]	"Peripheral Golgi protein GRASP65 is a target of mitotic polo-like kinase (Plk) and Cdc2." Lin C.-Y., Madsen M.L., Yarm F.R., Jang Y.-J., Liu X., Erikson R.L. Proc. Natl. Acad. Sci. U.S.A. 97:12589-12594(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216, MASS SPECTROMETRY.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ409349 mRNA. Translation: CAC35160.1. AK091168 mRNA. Translation: BAC03598.1. AK124755 mRNA. Translation: BAG54090.1. CH471055 Genomic DNA. Translation: EAW64554.1. BC008928 mRNA. Translation: AAH08928.1. BC075854 mRNA. Translation: AAH75854.1. BC103826 mRNA. Translation: AAI03827.1. BC103827 mRNA. Translation: AAI03828.1.
IPI	IPI00011074. IPI00456615. IPI00456616.
RefSeq	NP_114105.1. NM_031899.2.
UniGene	Hs.721946.
3D structure databases
ProteinModelPortal	Q9BQQ3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BQQ3. 1 interaction.
MINT	MINT-1185294.
STRING	9606.ENSP00000313869.
PTM databases
PhosphoSite	Q9BQQ3.
Polymorphism databases
DMDM	51316077.
Proteomic databases
PaxDb	Q9BQQ3.
PRIDE	Q9BQQ3.
Protocols and materials databases
DNASU	64689.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000319283; ENSP00000313869; ENSG00000114745. ENST00000431601; ENSP00000403552; ENSG00000114745. ENST00000452389; ENSP00000403167; ENSG00000114745.
GeneID	64689.
KEGG	hsa:64689.
UCSC	uc003ciw.1. human.
Organism-specific databases
CTD	64689.
GeneCards	GC03M039113.
HGNC	HGNC:16769. GORASP1.
HPA	HPA056283.
MIM	606867. gene.
neXtProt	NX_Q9BQQ3.
PharmGKB	PA28814.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5233.
HOGENOM	HOG000054196.
HOVERGEN	HBG051826.
InParanoid	Q9BQQ3.
OMA	SGPEDVC.
OrthoDB	EOG40P46V.
PhylomeDB	Q9BQQ3.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases.
Gene expression databases
ArrayExpress	Q9BQQ3.
Bgee	Q9BQQ3.
CleanEx	HS_GORASP1.
Genevestigator	Q9BQQ3.
GermOnline	ENSG00000114745. Homo sapiens.
Family and domain databases
InterPro	IPR024958. GRASP55/65_PDZ. IPR007583. GRASP55_65. IPR001478. PDZ. [Graphical view]
PANTHER	PTHR12893. PTHR12893. 1 hit.
Pfam	PF04495. GRASP55_65. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 2 hits.
PROSITE	PS50106. PDZ. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	64689.
NextBio	66611.
PMAP-CutDB	Q9BQQ3.
SOURCE	Search...

Entry information

Entry name

GORS1_HUMAN

Accession

Primary (citable) accession number: Q9BQQ3
Secondary accession number(s): B3KWC8 , Q3SYG7, Q8N272, Q96H42

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.