Q9BQI6 (ANR32_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ankyrin repeat domain-containing protein 32

Alternative name(s):
BRCT domain-containing protein 1

Gene names

Name:	ANKRD32
Synonyms:	BRCTD1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1058 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Contains 3 ANK repeats. Contains 2 BRCT domains.

Sequence caution

The sequence AAH54885.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAH54885.2 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH70332.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAH70332.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAI07572.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB66495.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	ANK repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1058	1058	Ankyrin repeat domain-containing protein 32		PRO_0000243906
Regions
	Domain	12 – 77	66	BRCT 1
	Domain	119 – 196	78	BRCT 2
	Repeat	806 – 836	31	ANK 1
	Repeat	840 – 869	30	ANK 2
	Repeat	874 – 903	30	ANK 3
Amino acid modifications
	Modified residue	44	1	Phosphothreonine Ref.8
	Modified residue	161	1	Phosphoserine Ref.7
	Modified residue	916	1	Phosphotyrosine Ref.6
Natural variations
	Natural variant	288	1	S → R. Corresponds to variant rs6891545 [ dbSNP | Ensembl ].		VAR_059120
Experimental info
	Sequence conflict	24	1	V → A in CAB43397. Ref.3
	Sequence conflict	103	1	Q → R in CAB43397. Ref.3
	Sequence conflict	182	1	F → S in AAH54885. Ref.2
	Sequence conflict	221	1	T → A in CAB43397. Ref.3
	Sequence conflict	328	1	H → Q in AAH70332. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9BQI6 [UniParc]. Last modified July 1, 2008. Version 2. Checksum: C624008C7CC25FE6	FASTA	1,058	121,050
	10 20 30 40 50 60 MEDGTPKHII QMTGFKMEEK EALVKLLLKL DCTFIKSEKY KNCTHLIAER LCKSEKFLAA 70 80 90 100 110 120 CAAGKWILTK DYIIHSAKSG RWLDETTYEW GYKIEKDSRY SPQMQSAPKR WREELKRTGA 130 140 150 160 170 180 PGAFHRWKVV LLVRTDKRSD SLIRVLEAGK ANVILPKSSP SGITHVIASN ARIKAEKEKD 190 200 210 220 230 240 NFKAPFYPIQ YLGDFLLEKE IQNDEDSQTN SVWTEHSNEE TNKDFRKDAG FLEMKGALRE 250 260 270 280 290 300 TMYRTQKEMQ NHEDVNVGSI LIQHHKKEKF SGSSKDLKFV KMRNTFGSHT YENQKEIKKK 310 320 330 340 350 360 DEDIQRSYTL RRKRKKGKES NCKKGVEHEK IKSTLRRHIY NRDQKEMKNS IFAEYAKESK 370 380 390 400 410 420 AMAIKTDVDV VEIKNTLRKH IYRAQAVRYN CIRIDKQPVY NVEVKNAEFP RGVLNLIESL 430 440 450 460 470 480 IEGHFFKEAI EELSTLQAHY IPPVCVLHAL LENVLQDNID TFSGRYFHIL SALLHLHPPW 490 500 510 520 530 540 KSPAMSRYYL ELFQCPTCMK GAWSLVEVLI RSCLFNESFC HQISENIGSK VLHLTLLKFF 550 560 570 580 590 600 FNLIESEVQH LSQKLYDWSD SQNLKITGKA MLLEIFWSGS ETSGLLTKPV NMLLEWTIYS 610 620 630 640 650 660 HKEKFKSNDV FKHELAYLLA GILGAAIDYW IFLGLKMGRN VMRHMSDDLG SYVSLSCDDF 670 680 690 700 710 720 SSQELEIFIC SFSSSWLQMF VAEAVFKKLC LQSSGSVSSE PLSLQKMVYS YLPALGKTGV 730 740 750 760 770 780 LGSGKIQVSK KIGQRPCFDS QRTLLMLNGT KQKQVEGLPE LLDLNLAKCS SSLKKLKKKS 790 800 810 820 830 840 EGELSCSKEN CPSVVKKMNF HKTNLKGETA LHRACINNQV EKLILLLSLP GIDINVKDNA 850 860 870 880 890 900 GWTPLHEACN YGNTVCVQEI LQRCPEVDLL TQVDGVTPLH DALSNGHVEI GKLLLQHGGP 910 920 930 940 950 960 VLLQQRNAKG ELPLDYVVSP QIKEELFAIT KIEDTVENFH AQAEKHFHYQ QLEFGSFLLS 970 980 990 1000 1010 1020 RMLLNFCSIF DLSSEFILAS KGLTHLNELL MACKSHKETT SVHTDWLLDL YAGNIKTLQK 1030 1040 1050 LPHILKELPE NLKVCPGVHT EALMITLEMM CRSVMEFS

References

[1]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-328. Tissue: Testis.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-267. Tissue: Brain.
[4]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 631-1058. Tissue: Amygdala.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 637-1058.
[6]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-916, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-161, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-44, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC008534 Genomic DNA. No translation available. AC025766 Genomic DNA. No translation available. BC054885 mRNA. Translation: AAH54885.2. Sequence problems. BC070332 mRNA. Translation: AAH70332.1. Sequence problems. BC107571 mRNA. Translation: AAI07572.1. Different initiation. AL050298 mRNA. Translation: CAB43397.2. AL136560 mRNA. Translation: CAB66495.1. Different initiation. CR533539 mRNA. Translation: CAG38570.1.
IPI	IPI00419518.
PIR	T08704.
RefSeq	NP_115666.2. NM_032290.3.
UniGene	Hs.657315.
3D structure databases
ProteinModelPortal	Q9BQI6.
SMR	Q9BQI6. Positions 6-209, 797-1046.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9BQI6.
PTM databases
PhosphoSite	Q9BQI6.
Polymorphism databases
DMDM	193806371.
Proteomic databases
PRIDE	Q9BQI6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265140; ENSP00000265140; ENSG00000133302.
GeneID	84250.
KEGG	hsa:84250.
UCSC	uc003kkr.2. human.
Organism-specific databases
CTD	84250.
GeneCards	GC05P094039.
H-InvDB	HIX0005038.
HGNC	HGNC:25408. ANKRD32.
HPA	HPA040793.
neXtProt	NX_Q9BQI6.
PharmGKB	PA134911523.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12184.
GeneTree	ENSGT00550000074487.
HOVERGEN	HBG103143.
InParanoid	Q9BQI6.
OMA	CPTCMKG.
OrthoDB	EOG4Z62PS.
PhylomeDB	Q9BQI6.
Gene expression databases
ArrayExpress	Q9BQI6.
Bgee	Q9BQI6.
CleanEx	HS_ANKRD32.
Genevestigator	Q9BQI6.
GermOnline	ENSG00000133302. Homo sapiens.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001357. BRCT. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 1 hit. PF12796. Ank_2. 1 hit. [Graphical view]
SMART	SM00248. ANK. 3 hits. SM00292. BRCT. 2 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF52113. BRCT. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 3 hits. PS50172. BRCT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	73744.

Entry information

Entry name

ANR32_HUMAN

Accession

Primary (citable) accession number: Q9BQI6
Secondary accession number(s): Q3B7K4 , Q6NSA5, Q6PHW9, Q9Y402

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.