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Q9BQI0 (AIF1L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Allograft inflammatory factor 1-like
Alternative name(s):
Ionized calcium-binding adapter molecule 2
Gene names
Name:AIF1L
Synonyms:C9orf58, IBA2
ORF Names:UNQ672/PRO1306
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length150 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Actin-binding protein that promotes actin bundling. May neither bind calcium nor depend on calcium for function. Ref.11

Subunit structure

Homodimer Potential. Monomer. Ref.11

Subcellular location

Cytoplasmcytoskeleton. Cell projectionruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with F-actin. Partially relocates to membrane ruffles in response to invading bacteria. Ref.11

Sequence similarities

Contains 2 EF-hand domains.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BQI0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BQI0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: R → RYCAGREPQLQRISCSQHSCLLALLFQ
Isoform 3 (identifier: Q9BQI0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     60-67: Missing.
Isoform 4 (identifier: Q9BQI0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     99-102: VSDT → SHDV
     103-150: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 150149Allograft inflammatory factor 1-like
PRO_0000073870

Regions

Domain47 – 8236EF-hand 1
Domain83 – 11735EF-hand 2; degenerate
Calcium binding60 – 71121 Potential

Amino acid modifications

Modified residue21N-acetylserine Ref.9 Ref.10
Modified residue21Phosphoserine Ref.10

Natural variations

Alternative sequence311R → RYCAGREPQLQRISCSQHSC LLALLFQ in isoform 2.
VSP_017150
Alternative sequence60 – 678Missing in isoform 3.
VSP_017151
Alternative sequence99 – 1024VSDT → SHDV in isoform 4.
VSP_017152
Alternative sequence103 – 15048Missing in isoform 4.
VSP_017153

Experimental info

Sequence conflict331F → L in BAB14269. Ref.3

Secondary structure

.................. 150
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 74614436B9DEB5F6

FASTA15017,068
        10         20         30         40         50         60 
MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD 

        70         80         90        100        110        120 
LNNEGEIDLM SLKRMMEKLG VPKTHLEMKK MISEVTGGVS DTISYRDFVN MMLGKRSAVL 

       130        140        150 
KLVMMFEGKA NESSPKPVGP PPERDIASLP 

« Hide

Isoform 2 [UniParc].

Checksum: 1F6AE2733802261E
Show »

FASTA17620,015
Isoform 3 [UniParc].

Checksum: F3A22B50146CBE82
Show »

FASTA14216,183
Isoform 4 [UniParc].

Checksum: 5B0A3F4AE73E042D
Show »

FASTA10211,817

References

« Hide 'large scale' references
[1]"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. expand/collapse author list , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Amygdala.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Kidney, Placenta, Teratocarcinoma and Trachea.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Amygdala.
[5]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Placenta.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[10]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Structural and functional characterization of human Iba proteins."
Schulze J.O., Quedenau C., Roske Y., Adam T., Schueler H., Behlke J., Turnbull A.P., Sievert V., Scheich C., Mueller U., Heinemann U., Buessow K.
FEBS J. 275:4627-4640(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS), FUNCTION, SUBCELLULAR LOCATION, LACK OF CALCIUM-BINDING, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL136566 mRNA. Translation: CAB66501.1.
AY359067 mRNA. Translation: AAQ89426.1.
AK022845 mRNA. Translation: BAB14269.1.
AK091912 mRNA. Translation: BAC03770.1.
AK128526 mRNA. Translation: BAC87480.1.
AK314600 mRNA. Translation: BAG37171.1.
AL833896 mRNA. Translation: CAD38752.1.
AL157938 Genomic DNA. Translation: CAI41102.1.
AL157938 Genomic DNA. Translation: CAI41103.1.
AL157938 Genomic DNA. Translation: CAI41104.1.
CH471090 Genomic DNA. Translation: EAW87958.1.
BC021253 mRNA. Translation: AAH21253.1.
CCDSCCDS55348.1. [Q9BQI0-2]
CCDS55349.1. [Q9BQI0-4]
CCDS6939.1. [Q9BQI0-1]
RefSeqNP_001172024.1. NM_001185095.1. [Q9BQI0-2]
NP_001172025.1. NM_001185096.1. [Q9BQI0-4]
NP_113614.1. NM_031426.3. [Q9BQI0-1]
UniGeneHs.4944.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JJZX-ray2.15B/C/D1-150[»]
2VTGX-ray2.45A1-150[»]
ProteinModelPortalQ9BQI0.
SMRQ9BQI0. Positions 13-127.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123676. 4 interactions.
MINTMINT-1137255.

PTM databases

PhosphoSiteQ9BQI0.

Proteomic databases

MaxQBQ9BQI0.
PaxDbQ9BQI0.
PRIDEQ9BQI0.

Protocols and materials databases

DNASU83543.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000247291; ENSP00000247291; ENSG00000126878. [Q9BQI0-1]
ENST00000372300; ENSP00000361374; ENSG00000126878. [Q9BQI0-4]
ENST00000372302; ENSP00000361376; ENSG00000126878. [Q9BQI0-3]
ENST00000372309; ENSP00000361383; ENSG00000126878. [Q9BQI0-2]
GeneID83543.
KEGGhsa:83543.
UCSCuc004cab.2. human. [Q9BQI0-1]
uc004cad.2. human. [Q9BQI0-2]
uc004cae.2. human. [Q9BQI0-4]

Organism-specific databases

CTD83543.
GeneCardsGC09P133971.
HGNCHGNC:28904. AIF1L.
HPAHPA020522.
neXtProtNX_Q9BQI0.
PharmGKBPA164715250.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG262998.
HOGENOMHOG000231928.
HOVERGENHBG004002.
PhylomeDBQ9BQI0.
TreeFamTF320736.

Gene expression databases

ArrayExpressQ9BQI0.
BgeeQ9BQI0.
CleanExHS_AIF1L.
GenevestigatorQ9BQI0.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR028453. Aif1_like.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERPTHR10356:SF3. PTHR10356:SF3. 1 hit.
PROSITEPS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAIF1L. human.
EvolutionaryTraceQ9BQI0.
GeneWikiC9orf58.
GenomeRNAi83543.
NextBio72467.
PROQ9BQI0.

Entry information

Entry nameAIF1L_HUMAN
AccessionPrimary (citable) accession number: Q9BQI0
Secondary accession number(s): B2RBC4 expand/collapse secondary AC list , Q6ZR40, Q8NAX7, Q8WU47, Q9H9G0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM