Reviewed, UniProtKB/Swiss-Prot Q9BQI0 (AIF1L_HUMAN)

Names and origin

Protein names

Recommended name:
    Allograft inflammatory factor 1-like
Alternative name(s):
    Ionized calcium-binding adapter molecule 2

Gene names

Name:	AIF1L
Synonyms:	C9orf58, IBA2
ORF Names:	UNQ672/PRO1306

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Actin-binding protein that promotes actin bundling. May neither bind calcium nor depend on calcium for function. Ref.8
Subunit structure	Homodimer Potential. Monomer.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with F-actin. Partially relocates to membrane ruffles in response to invading bacteria. Ref.8
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Actin-binding Calcium
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular component	actin cytoskeleton Inferred from direct assay. Source: LIFEdb focal adhesion Inferred from direct assay. Source: LIFEdb
Molecular function	actin filament binding Ref.8 Inferred from direct assay. Source: UniProtKB calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BQI0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BQI0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: R → RYCAGREPQLQRISCSQHSCLLALLFQ

Isoform 3 (identifier: Q9BQI0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     60-67: Missing.

Isoform 4 (identifier: Q9BQI0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     99-102: VSDT → SHDV
     103-150: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 150	150	Allograft inflammatory factor 1-like		PRO_0000073870
Regions
	Domain	47 – 82	36	EF-hand 1
	Domain	83 – 117	35	EF-hand 2; degenerate
	Calcium binding	60 – 71	12	1 Potential
Natural variations
	Alternative sequence	31	1	R → RYCAGREPQLQRISCSQHSC LLALLFQ in isoform 2.		VSP_017150
	Alternative sequence	60 – 67	8	Missing in isoform 3.		VSP_017151
	Alternative sequence	99 – 102	4	VSDT → SHDV in isoform 4.		VSP_017152
	Alternative sequence	103 – 150	48	Missing in isoform 4.		VSP_017153
Experimental info
	Sequence conflict	33	1	F → L in BAB14269. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 74614436B9DEB5F6	FASTA	150	17,068
	10 20 30 40 50 60 MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD 70 80 90 100 110 120 LNNEGEIDLM SLKRMMEKLG VPKTHLEMKK MISEVTGGVS DTISYRDFVN MMLGKRSAVL 130 140 150 KLVMMFEGKA NESSPKPVGP PPERDIASLP
	Isoform 2. Checksum: 1F6AE2733802261E	FASTA	176	20,015
	10 20 30 40 50 60 MSGELSNRFQ GGKAFGLLKA RQERRLAEIN RYCAGREPQL QRISCSQHSC LLALLFQEFL 70 80 90 100 110 120 CDQKYSDEEN LPEKLTAFKE KYMEFDLNNE GEIDLMSLKR MMEKLGVPKT HLEMKKMISE 130 140 150 160 170 VTGGVSDTIS YRDFVNMMLG KRSAVLKLVM MFEGKANESS PKPVGPPPER DIASLP
	Isoform 3. Checksum: F3A22B50146CBE82	FASTA	142	16,183
	10 20 30 40 50 60 MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD 70 80 90 100 110 120 LMSLKRMMEK LGVPKTHLEM KKMISEVTGG VSDTISYRDF VNMMLGKRSA VLKLVMMFEG 130 140 KANESSPKPV GPPPERDIAS LP
	Isoform 4. Checksum: 5B0A3F4AE73E042D	FASTA	102	11,817
	10 20 30 40 50 60 MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD 70 80 90 100 LNNEGEIDLM SLKRMMEKLG VPKTHLEMKK MISEVTGGSH DV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Kidney, Placenta, Teratocarcinoma and Trachea.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala.
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Placenta.
[8]	"Structural and functional characterization of human Iba proteins." Schulze J.O., Quedenau C., Roske Y., Adam T., Schueler H., Behlke J., Turnbull A.P., Sievert V., Scheich C., Mueller U., Heinemann U., Buessow K. FEBS J. 275:4627-4640(2008) [PubMed: 18699778] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS), FUNCTION, SUBCELLULAR LOCATION, LACK OF CALCIUM-BINDING, SUBUNIT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AL136566 mRNA. Translation: CAB66501.1.
AY359067 mRNA. Translation: AAQ89426.1.
AK022845 mRNA. Translation: BAB14269.1.
AK091912 mRNA. Translation: BAC03770.1.
AK128526 mRNA. Translation: BAC87480.1.
AK314600 mRNA. Translation: BAG37171.1.
AL833896 mRNA. Translation: CAD38752.1.
AL157938 Genomic DNA. Translation: CAI41102.1.
AL157938 Genomic DNA. Translation: CAI41103.1.
AL157938 Genomic DNA. Translation: CAI41104.1.
CH471090 Genomic DNA. Translation: EAW87958.1.
BC021253 mRNA. Translation: AAH21253.1.

IPI

IPI00034205.
IPI00549277.
IPI00553214.
IPI00643837.

RefSeq

NP_113614.1.

UniGene

Hs.4944

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VTG	X-ray	2.45	A	1-150	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9BQI0. 1 interaction.

PTM databases

PhosphoSite

Q9BQI0.

Proteomic databases

PRIDE

Q9BQI0.

Genome annotation databases

Ensembl

ENST00000247291; ENSP00000247291; ENSG00000126878; Homo sapiens. [Genome view]

GeneID

83543.

KEGG

hsa:83543.

UCSC

uc004cab.1. human.
uc004cae.1. human.

Organism-specific databases

CTD

83543.

GeneCards

GC09P132962.

H-InvDB

HIX0008478.

HGNC

HGNC:28904. AIF1L.

PharmGKB

PA128394733.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q9BQI0.

OrthoDB

EOG9V722Z.

Gene expression databases

ArrayExpress

Q9BQI0.

Bgee

Q9BQI0.

CleanEx

HS_AIF1L.

Genevestigator

Q9BQI0.

GermOnline

ENSG00000126878. Homo sapiens.

Family and domain databases

InterPro

IPR011992. EF-Hand_type.
IPR018249. EF_HAND_2.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

PROSITE

PS00018. EF_HAND_1. False negative.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

72467.

Entry information

Entry name

AIF1L_HUMAN

Accession

Primary (citable) accession number: Q9BQI0
Secondary accession number(s): B2RBC4 , Q6ZR40, Q8NAX7, Q8WU47, Q9H9G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	June 1, 2001
Last modified:	November 24, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.