Q9BQI0 (AIF1L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Allograft inflammatory factor 1-like

Alternative name(s):
Ionized calcium-binding adapter molecule 2

Gene names

Name:	AIF1L
Synonyms:	C9orf58, IBA2
ORF Names:	UNQ672/PRO1306

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Actin-binding protein that promotes actin bundling. May neither bind calcium nor depend on calcium for function. Ref.10
Subunit structure	Homodimer Potential. Monomer. Ref.10
Subcellular location	Cytoplasm › cytoskeleton. Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with F-actin. Partially relocates to membrane ruffles in response to invading bacteria. Ref.10
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Actin-binding Calcium Metal-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	actin cytoskeleton Inferred from direct assay. Source: LIFEdb cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW focal adhesion Inferred from direct assay. Source: LIFEdb protein complex Inferred from electronic annotation. Source: Compara ruffle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	actin filament binding Inferred from direct assay Ref.10. Source: UniProtKB calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BQI0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BQI0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     31-31: R → RYCAGREPQLQRISCSQHSCLLALLFQ

Isoform 3 (identifier: Q9BQI0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     60-67: Missing.

Isoform 4 (identifier: Q9BQI0-4)

The sequence of this isoform differs from the canonical sequence as follows:
     99-102: VSDT → SHDV
     103-150: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.9

Chain

2 – 150

149

Allograft inflammatory factor 1-like

PRO_0000073870

Regions

Domain

47 – 82

36

EF-hand 1

Domain

83 – 117

35

EF-hand 2; degenerate

Calcium binding

60 – 71

12

1 Potential

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.9

Modified residue

2

1

Phosphoserine Ref.9

Natural variations

Alternative sequence

31

1

R → RYCAGREPQLQRISCSQHSC LLALLFQ in isoform 2.

VSP_017150

Alternative sequence

60 – 67

8

Missing in isoform 3.

VSP_017151

Alternative sequence

99 – 102

4

VSDT → SHDV in isoform 4.

VSP_017152

Alternative sequence

103 – 150

48

Missing in isoform 4.

VSP_017153

Experimental info

Sequence conflict

33

1

F → L in BAB14269. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

150

Helix Strand Turn

Details...

Helix

17 – 34

18

Helix

37 – 39

3

Helix

45 – 56

12

Helix

69 – 78

10

Helix

85 – 96

12

Beta strand

101 – 103

3

Helix

105 – 113

9

Beta strand

114 – 116

3

Helix

119 – 124

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 74614436B9DEB5F6	FASTA	150	17,068
	10 20 30 40 50 60 MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD 70 80 90 100 110 120 LNNEGEIDLM SLKRMMEKLG VPKTHLEMKK MISEVTGGVS DTISYRDFVN MMLGKRSAVL 130 140 150 KLVMMFEGKA NESSPKPVGP PPERDIASLP
	Isoform 2 [UniParc]. Checksum: 1F6AE2733802261E	FASTA	176	20,015
	10 20 30 40 50 60 MSGELSNRFQ GGKAFGLLKA RQERRLAEIN RYCAGREPQL QRISCSQHSC LLALLFQEFL 70 80 90 100 110 120 CDQKYSDEEN LPEKLTAFKE KYMEFDLNNE GEIDLMSLKR MMEKLGVPKT HLEMKKMISE 130 140 150 160 170 VTGGVSDTIS YRDFVNMMLG KRSAVLKLVM MFEGKANESS PKPVGPPPER DIASLP
	Isoform 3 [UniParc]. Checksum: F3A22B50146CBE82	FASTA	142	16,183
	10 20 30 40 50 60 MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD 70 80 90 100 110 120 LMSLKRMMEK LGVPKTHLEM KKMISEVTGG VSDTISYRDF VNMMLGKRSA VLKLVMMFEG 130 140 KANESSPKPV GPPPERDIAS LP
	Isoform 4 [UniParc]. Checksum: 5B0A3F4AE73E042D	FASTA	102	11,817
	10 20 30 40 50 60 MSGELSNRFQ GGKAFGLLKA RQERRLAEIN REFLCDQKYS DEENLPEKLT AFKEKYMEFD 70 80 90 100 LNNEGEIDLM SLKRMMEKLG VPKTHLEMKK MISEVTGGSH DV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Kidney, Placenta, Teratocarcinoma and Trachea.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Amygdala.
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Placenta.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
[10]	"Structural and functional characterization of human Iba proteins." Schulze J.O., Quedenau C., Roske Y., Adam T., Schueler H., Behlke J., Turnbull A.P., Sievert V., Scheich C., Mueller U., Heinemann U., Buessow K. FEBS J. 275:4627-4640(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS), FUNCTION, SUBCELLULAR LOCATION, LACK OF CALCIUM-BINDING, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL136566 mRNA. Translation: CAB66501.1.
AY359067 mRNA. Translation: AAQ89426.1.
AK022845 mRNA. Translation: BAB14269.1.
AK091912 mRNA. Translation: BAC03770.1.
AK128526 mRNA. Translation: BAC87480.1.
AK314600 mRNA. Translation: BAG37171.1.
AL833896 mRNA. Translation: CAD38752.1.
AL157938 Genomic DNA. Translation: CAI41102.1.
AL157938 Genomic DNA. Translation: CAI41103.1.
AL157938 Genomic DNA. Translation: CAI41104.1.
CH471090 Genomic DNA. Translation: EAW87958.1.
BC021253 mRNA. Translation: AAH21253.1.

IPI

IPI00034205.
IPI00549277.
IPI00553214.
IPI00643837.

RefSeq

NP_001172024.1. NM_001185095.1.
NP_001172025.1. NM_001185096.1.
NP_113614.1. NM_031426.3.

UniGene

Hs.4944.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JJZ	X-ray	2.15	A/B/C/D	1-150	[»]
2VTG	X-ray	2.45	A	1-150	[»]

ProteinModelPortal

Q9BQI0.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-1137255.

PTM databases

PhosphoSite

Q9BQI0.

Polymorphism databases

DMDM

74752240.

Proteomic databases

PaxDb

Q9BQI0.

PRIDE

Q9BQI0.

Protocols and materials databases

DNASU

83543.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000247291; ENSP00000247291; ENSG00000126878.
ENST00000372300; ENSP00000361374; ENSG00000126878.
ENST00000372302; ENSP00000361376; ENSG00000126878.
ENST00000372309; ENSP00000361383; ENSG00000126878.

GeneID

83543.

KEGG

hsa:83543.

UCSC

uc004cab.2. human.
uc004cad.2. human.
uc004cae.2. human.

Organism-specific databases

CTD

83543.

GeneCards

GC09P133971.

HGNC

HGNC:28904. AIF1L.

neXtProt

NX_Q9BQI0.

PharmGKB

PA164715250.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG262998.

HOGENOM

HOG000231928.

HOVERGEN

HBG004002.

Gene expression databases

ArrayExpress

Q9BQI0.

Bgee

Q9BQI0.

CleanEx

HS_AIF1L.

Genevestigator

Q9BQI0.

GermOnline

ENSG00000126878. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR002048. EF_hand_dom.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. False negative.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

AIF1L. human.

EvolutionaryTrace

Q9BQI0.

GenomeRNAi

83543.

NextBio

72467.

Entry information

Entry name

AIF1L_HUMAN

Accession

Primary (citable) accession number: Q9BQI0
Secondary accession number(s): B2RBC4 , Q6ZR40, Q8NAX7, Q8WU47, Q9H9G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	June 1, 2001
Last modified:	May 29, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.