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Protein

Peroxisomal NADH pyrophosphatase NUDT12

Gene

NUDT12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)+, ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle.1 Publication

Catalytic activityi

NAD+ + H2O = AMP + NMN.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Kineticsi

  1. KM=11 µM for NADH1 Publication
  2. KM=16 µM for NADPH1 Publication
  3. KM=190 µM for NAD1 Publication

    pH dependencei

    Optimum pH is 8-9.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi370 – 3701Magnesium or manganeseBy similarity
    Metal bindingi374 – 3741Magnesium or manganeseBy similarity

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • NAD+ diphosphatase activity Source: UniProtKB
    • NADH pyrophosphatase activity Source: UniProtKB

    GO - Biological processi

    • NAD catabolic process Source: UniProtKB
    • NADP catabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal NADH pyrophosphatase NUDT12 (EC:3.6.1.22)
    Alternative name(s):
    Nucleoside diphosphate-linked moiety X motif 12
    Short name:
    Nudix motif 12
    Gene namesi
    Name:NUDT12
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:18826. NUDT12.

    Subcellular locationi

    • Peroxisome 1 Publication

    GO - Cellular componenti

    • nucleus Source: LIFEdb
    • peroxisome Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi460 – 4623Missing : Abolishes localization to peroxisomes. 1 Publication

    Organism-specific databases

    PharmGKBiPA38699.

    Polymorphism and mutation databases

    BioMutaiNUDT12.
    DMDMi68565930.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462Peroxisomal NADH pyrophosphatase NUDT12PRO_0000056956Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei185 – 1851N6-succinyllysineBy similarity
    Modified residuei292 – 2921N6-succinyllysineBy similarity

    Proteomic databases

    MaxQBiQ9BQG2.
    PaxDbiQ9BQG2.
    PRIDEiQ9BQG2.

    PTM databases

    PhosphoSiteiQ9BQG2.

    Expressioni

    Gene expression databases

    BgeeiQ9BQG2.
    CleanExiHS_NUDT12.
    ExpressionAtlasiQ9BQG2. baseline and differential.
    GenevisibleiQ9BQG2. HS.

    Organism-specific databases

    HPAiHPA045449.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BLMHQ138673EBI-10230612,EBI-718504

    Protein-protein interaction databases

    BioGridi123690. 9 interactions.
    IntActiQ9BQG2. 1 interaction.
    MINTiMINT-3060539.
    STRINGi9606.ENSP00000230792.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BQG2.
    SMRiQ9BQG2. Positions 44-98, 206-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati11 – 4030ANK 1Add
    BLAST
    Repeati45 – 7430ANK 2Add
    BLAST
    Repeati78 – 9821ANK 3Add
    BLAST
    Domaini319 – 453135Nudix hydrolasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi355 – 37622Nudix boxAdd
    BLAST
    Motifi460 – 4623Microbody targeting signalCurated

    Sequence similaritiesi

    Belongs to the Nudix hydrolase family. NudC subfamily.Curated
    Contains 3 ANK repeats.PROSITE-ProRule annotation
    Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG2816.
    GeneTreeiENSGT00530000063600.
    HOGENOMiHOG000247937.
    HOVERGENiHBG054394.
    InParanoidiQ9BQG2.
    KOiK03426.
    OMAiEEGGYKR.
    OrthoDBiEOG7N0C55.
    PhylomeDBiQ9BQG2.
    TreeFamiTF106352.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.90.79.10. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR015375. NADH_PPase-like_N.
    IPR020084. NUDIX_hydrolase_CS.
    IPR000086. NUDIX_hydrolase_dom.
    IPR015797. NUDIX_hydrolase_dom-like.
    IPR015376. Znr_NADH_PPase.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF00293. NUDIX. 1 hit.
    PF09296. NUDIX-like. 1 hit.
    PF09297. zf-NADH-PPase. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 3 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF55811. SSF55811. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS51462. NUDIX. 1 hit.
    PS00893. NUDIX_BOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9BQG2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSVKRSLKQ EIVTQFHCSA AEGDIAKLTG ILSHSPSLLN ETSENGWTAL
    60 70 80 90 100
    MYAARNGHPE IVQFLLEKGC DRSIVNKSRQ TALDIAVFWG YKHIANLLAT
    110 120 130 140 150
    AKGGKKPWFL TNEVEECENY FSKTLLDRKS EKRNNSDWLL AKESHPATVF
    160 170 180 190 200
    ILFSDLNPLV TLGGNKESFQ QPEVRLCQLN YTDIKDYLAQ PEKITLIFLG
    210 220 230 240 250
    VELEIKDKLL NYAGEVPREE EDGLVAWFAL GIDPIAAEEF KQRHENCYFL
    260 270 280 290 300
    HPPMPALLQL KEKEAGVVAQ ARSVLAWHSR YKFCPTCGNA TKIEEGGYKR
    310 320 330 340 350
    LCLKEDCPSL NGVHNTSYPR VDPVVIMQVI HPDGTKCLLG RQKRFPPGMF
    360 370 380 390 400
    TCLAGFIEPG ETIEDAVRRE VEEESGVKVG HVQYVACQPW PMPSSLMIGC
    410 420 430 440 450
    LALAVSTEIK VDKNEIEDAR WFTREQVLDV LTKGKQQAFF VPPSRAIAHQ
    460
    LIKHWIRINP NL
    Length:462
    Mass (Da):52,076
    Last modified:June 1, 2001 - v1
    Checksum:i1A470B93707DB1D5
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti129 – 1291K → E.
    Corresponds to variant rs35903418 [ dbSNP | Ensembl ].
    VAR_034157
    Natural varianti235 – 2351I → V.
    Corresponds to variant rs34468716 [ dbSNP | Ensembl ].
    VAR_034158

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL136592 mRNA. Translation: CAB66527.1.
    AK098066 mRNA. Translation: BAG53574.1.
    CH471086 Genomic DNA. Translation: EAW49073.1.
    BC026748 mRNA. Translation: AAH26748.1.
    BC041099 mRNA. Translation: AAH41099.1.
    CCDSiCCDS4096.1.
    RefSeqiNP_001287670.1. NM_001300741.1.
    NP_113626.1. NM_031438.3.
    XP_005272152.1. XM_005272095.1.
    UniGeneiHs.434289.

    Genome annotation databases

    EnsembliENST00000230792; ENSP00000230792; ENSG00000112874.
    GeneIDi83594.
    KEGGihsa:83594.
    UCSCiuc003koi.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL136592 mRNA. Translation: CAB66527.1.
    AK098066 mRNA. Translation: BAG53574.1.
    CH471086 Genomic DNA. Translation: EAW49073.1.
    BC026748 mRNA. Translation: AAH26748.1.
    BC041099 mRNA. Translation: AAH41099.1.
    CCDSiCCDS4096.1.
    RefSeqiNP_001287670.1. NM_001300741.1.
    NP_113626.1. NM_031438.3.
    XP_005272152.1. XM_005272095.1.
    UniGeneiHs.434289.

    3D structure databases

    ProteinModelPortaliQ9BQG2.
    SMRiQ9BQG2. Positions 44-98, 206-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi123690. 9 interactions.
    IntActiQ9BQG2. 1 interaction.
    MINTiMINT-3060539.
    STRINGi9606.ENSP00000230792.

    PTM databases

    PhosphoSiteiQ9BQG2.

    Polymorphism and mutation databases

    BioMutaiNUDT12.
    DMDMi68565930.

    Proteomic databases

    MaxQBiQ9BQG2.
    PaxDbiQ9BQG2.
    PRIDEiQ9BQG2.

    Protocols and materials databases

    DNASUi83594.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000230792; ENSP00000230792; ENSG00000112874.
    GeneIDi83594.
    KEGGihsa:83594.
    UCSCiuc003koi.3. human.

    Organism-specific databases

    CTDi83594.
    GeneCardsiGC05M102884.
    HGNCiHGNC:18826. NUDT12.
    HPAiHPA045449.
    MIMi609232. gene.
    neXtProtiNX_Q9BQG2.
    PharmGKBiPA38699.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiCOG2816.
    GeneTreeiENSGT00530000063600.
    HOGENOMiHOG000247937.
    HOVERGENiHBG054394.
    InParanoidiQ9BQG2.
    KOiK03426.
    OMAiEEGGYKR.
    OrthoDBiEOG7N0C55.
    PhylomeDBiQ9BQG2.
    TreeFamiTF106352.

    Miscellaneous databases

    GeneWikiiNUDT12.
    GenomeRNAii83594.
    NextBioi72525.
    PROiQ9BQG2.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9BQG2.
    CleanExiHS_NUDT12.
    ExpressionAtlasiQ9BQG2. baseline and differential.
    GenevisibleiQ9BQG2. HS.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.90.79.10. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR015375. NADH_PPase-like_N.
    IPR020084. NUDIX_hydrolase_CS.
    IPR000086. NUDIX_hydrolase_dom.
    IPR015797. NUDIX_hydrolase_dom-like.
    IPR015376. Znr_NADH_PPase.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF00293. NUDIX. 1 hit.
    PF09296. NUDIX-like. 1 hit.
    PF09297. zf-NADH-PPase. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 3 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF55811. SSF55811. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS51462. NUDIX. 1 hit.
    PS00893. NUDIX_BOX. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Amygdala.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Trachea.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye and Ovary.
    5. "Mammalian NADH diphosphatases of the Nudix family: cloning and characterization of the human peroxisomal NUDT12 protein."
      Abdelraheim S.R., Spiller D.G., McLennan A.G.
      Biochem. J. 374:329-335(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MUTAGENESIS OF 460-PRO--LEU-462.

    Entry informationi

    Entry nameiNUD12_HUMAN
    AccessioniPrimary (citable) accession number: Q9BQG2
    Secondary accession number(s): B3KUW2, Q8TAL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: June 1, 2001
    Last modified: July 22, 2015
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.