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Protein

Peroxisomal NADH pyrophosphatase NUDT12

Gene

NUDT12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)+, ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle.1 Publication

Catalytic activityi

NAD(H) + H2O = AMP + NMN(H).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Kineticsi

  1. KM=11 µM for NADH1 Publication
  2. KM=16 µM for NADPH1 Publication
  3. KM=190 µM for NAD1 Publication

    pH dependencei

    Optimum pH is 8-9.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi370Magnesium or manganeseBy similarity1
    Metal bindingi374Magnesium or manganeseBy similarity1

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • NAD+ diphosphatase activity Source: UniProtKB
    • NADH pyrophosphatase activity Source: UniProtKB

    GO - Biological processi

    • NAD catabolic process Source: UniProtKB
    • NADP catabolic process Source: UniProtKB
    • nicotinamide metabolic process Source: Reactome
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD, NADP

    Enzyme and pathway databases

    BioCyciZFISH:HS12784-MONOMER.
    ReactomeiR-HSA-197264. Nicotinamide salvaging.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal NADH pyrophosphatase NUDT12 (EC:3.6.1.22)
    Alternative name(s):
    Nucleoside diphosphate-linked moiety X motif 12
    Short name:
    Nudix motif 12
    Gene namesi
    Name:NUDT12
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:18826. NUDT12.

    Subcellular locationi

    • Peroxisome 1 Publication

    GO - Cellular componenti

    • nucleus Source: LIFEdb
    • peroxisomal matrix Source: Reactome
    • peroxisome Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi460 – 462Missing : Abolishes localization to peroxisomes. 1 Publication3

    Organism-specific databases

    DisGeNETi83594.
    OpenTargetsiENSG00000112874.
    PharmGKBiPA38699.

    Polymorphism and mutation databases

    BioMutaiNUDT12.
    DMDMi68565930.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000569561 – 462Peroxisomal NADH pyrophosphatase NUDT12Add BLAST462

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei185N6-succinyllysineBy similarity1
    Modified residuei292N6-succinyllysineBy similarity1

    Proteomic databases

    EPDiQ9BQG2.
    MaxQBiQ9BQG2.
    PaxDbiQ9BQG2.
    PeptideAtlasiQ9BQG2.
    PRIDEiQ9BQG2.

    PTM databases

    iPTMnetiQ9BQG2.
    PhosphoSitePlusiQ9BQG2.

    Expressioni

    Gene expression databases

    BgeeiENSG00000112874.
    CleanExiHS_NUDT12.
    ExpressionAtlasiQ9BQG2. baseline and differential.
    GenevisibleiQ9BQG2. HS.

    Organism-specific databases

    HPAiHPA045449.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BLMHQ138673EBI-10230612,EBI-718504

    Protein-protein interaction databases

    BioGridi123690. 12 interactors.
    IntActiQ9BQG2. 1 interactor.
    MINTiMINT-3060539.
    STRINGi9606.ENSP00000230792.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BQG2.
    SMRiQ9BQG2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Repeati11 – 40ANK 1Add BLAST30
    Repeati45 – 74ANK 2Add BLAST30
    Repeati78 – 98ANK 3Add BLAST21
    Domaini319 – 453Nudix hydrolasePROSITE-ProRule annotationAdd BLAST135

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi355 – 376Nudix boxAdd BLAST22
    Motifi460 – 462Microbody targeting signalCurated3

    Sequence similaritiesi

    Belongs to the Nudix hydrolase family. NudC subfamily.Curated
    Contains 3 ANK repeats.PROSITE-ProRule annotation
    Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiKOG0504. Eukaryota.
    KOG3084. Eukaryota.
    COG2816. LUCA.
    GeneTreeiENSGT00530000063600.
    HOGENOMiHOG000247937.
    HOVERGENiHBG054394.
    InParanoidiQ9BQG2.
    KOiK03426.
    OMAiQPEVRLC.
    OrthoDBiEOG091G0SLC.
    PhylomeDBiQ9BQG2.
    TreeFamiTF106352.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.90.79.10. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR015375. NADH_PPase-like_N.
    IPR020084. NUDIX_hydrolase_CS.
    IPR000086. NUDIX_hydrolase_dom.
    IPR015797. NUDIX_hydrolase_dom-like.
    IPR015376. Znr_NADH_PPase.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF00293. NUDIX. 1 hit.
    PF09296. NUDIX-like. 1 hit.
    PF09297. zf-NADH-PPase. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 3 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF55811. SSF55811. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS51462. NUDIX. 1 hit.
    PS00893. NUDIX_BOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9BQG2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSVKRSLKQ EIVTQFHCSA AEGDIAKLTG ILSHSPSLLN ETSENGWTAL
    60 70 80 90 100
    MYAARNGHPE IVQFLLEKGC DRSIVNKSRQ TALDIAVFWG YKHIANLLAT
    110 120 130 140 150
    AKGGKKPWFL TNEVEECENY FSKTLLDRKS EKRNNSDWLL AKESHPATVF
    160 170 180 190 200
    ILFSDLNPLV TLGGNKESFQ QPEVRLCQLN YTDIKDYLAQ PEKITLIFLG
    210 220 230 240 250
    VELEIKDKLL NYAGEVPREE EDGLVAWFAL GIDPIAAEEF KQRHENCYFL
    260 270 280 290 300
    HPPMPALLQL KEKEAGVVAQ ARSVLAWHSR YKFCPTCGNA TKIEEGGYKR
    310 320 330 340 350
    LCLKEDCPSL NGVHNTSYPR VDPVVIMQVI HPDGTKCLLG RQKRFPPGMF
    360 370 380 390 400
    TCLAGFIEPG ETIEDAVRRE VEEESGVKVG HVQYVACQPW PMPSSLMIGC
    410 420 430 440 450
    LALAVSTEIK VDKNEIEDAR WFTREQVLDV LTKGKQQAFF VPPSRAIAHQ
    460
    LIKHWIRINP NL
    Length:462
    Mass (Da):52,076
    Last modified:June 1, 2001 - v1
    Checksum:i1A470B93707DB1D5
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_034157129K → E.Corresponds to variant rs35903418dbSNPEnsembl.1
    Natural variantiVAR_034158235I → V.Corresponds to variant rs34468716dbSNPEnsembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL136592 mRNA. Translation: CAB66527.1.
    AK098066 mRNA. Translation: BAG53574.1.
    CH471086 Genomic DNA. Translation: EAW49073.1.
    BC026748 mRNA. Translation: AAH26748.1.
    BC041099 mRNA. Translation: AAH41099.1.
    CCDSiCCDS4096.1.
    RefSeqiNP_001287670.1. NM_001300741.1.
    NP_113626.1. NM_031438.3.
    XP_005272152.1. XM_005272095.1.
    UniGeneiHs.434289.

    Genome annotation databases

    EnsembliENST00000230792; ENSP00000230792; ENSG00000112874.
    GeneIDi83594.
    KEGGihsa:83594.
    UCSCiuc003koi.4. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL136592 mRNA. Translation: CAB66527.1.
    AK098066 mRNA. Translation: BAG53574.1.
    CH471086 Genomic DNA. Translation: EAW49073.1.
    BC026748 mRNA. Translation: AAH26748.1.
    BC041099 mRNA. Translation: AAH41099.1.
    CCDSiCCDS4096.1.
    RefSeqiNP_001287670.1. NM_001300741.1.
    NP_113626.1. NM_031438.3.
    XP_005272152.1. XM_005272095.1.
    UniGeneiHs.434289.

    3D structure databases

    ProteinModelPortaliQ9BQG2.
    SMRiQ9BQG2.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi123690. 12 interactors.
    IntActiQ9BQG2. 1 interactor.
    MINTiMINT-3060539.
    STRINGi9606.ENSP00000230792.

    PTM databases

    iPTMnetiQ9BQG2.
    PhosphoSitePlusiQ9BQG2.

    Polymorphism and mutation databases

    BioMutaiNUDT12.
    DMDMi68565930.

    Proteomic databases

    EPDiQ9BQG2.
    MaxQBiQ9BQG2.
    PaxDbiQ9BQG2.
    PeptideAtlasiQ9BQG2.
    PRIDEiQ9BQG2.

    Protocols and materials databases

    DNASUi83594.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000230792; ENSP00000230792; ENSG00000112874.
    GeneIDi83594.
    KEGGihsa:83594.
    UCSCiuc003koi.4. human.

    Organism-specific databases

    CTDi83594.
    DisGeNETi83594.
    GeneCardsiNUDT12.
    HGNCiHGNC:18826. NUDT12.
    HPAiHPA045449.
    MIMi609232. gene.
    neXtProtiNX_Q9BQG2.
    OpenTargetsiENSG00000112874.
    PharmGKBiPA38699.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG0504. Eukaryota.
    KOG3084. Eukaryota.
    COG2816. LUCA.
    GeneTreeiENSGT00530000063600.
    HOGENOMiHOG000247937.
    HOVERGENiHBG054394.
    InParanoidiQ9BQG2.
    KOiK03426.
    OMAiQPEVRLC.
    OrthoDBiEOG091G0SLC.
    PhylomeDBiQ9BQG2.
    TreeFamiTF106352.

    Enzyme and pathway databases

    BioCyciZFISH:HS12784-MONOMER.
    ReactomeiR-HSA-197264. Nicotinamide salvaging.

    Miscellaneous databases

    GeneWikiiNUDT12.
    GenomeRNAii83594.
    PROiQ9BQG2.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000112874.
    CleanExiHS_NUDT12.
    ExpressionAtlasiQ9BQG2. baseline and differential.
    GenevisibleiQ9BQG2. HS.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.90.79.10. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR015375. NADH_PPase-like_N.
    IPR020084. NUDIX_hydrolase_CS.
    IPR000086. NUDIX_hydrolase_dom.
    IPR015797. NUDIX_hydrolase_dom-like.
    IPR015376. Znr_NADH_PPase.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF00293. NUDIX. 1 hit.
    PF09296. NUDIX-like. 1 hit.
    PF09297. zf-NADH-PPase. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 3 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF55811. SSF55811. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS51462. NUDIX. 1 hit.
    PS00893. NUDIX_BOX. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNUD12_HUMAN
    AccessioniPrimary (citable) accession number: Q9BQG2
    Secondary accession number(s): B3KUW2, Q8TAL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: June 1, 2001
    Last modified: November 2, 2016
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.