Sentrin-specific protease 7 - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    Sentrin-specific protease 7
    EC=3.4.22.-
Alternative name(s):
    Sentrin/SUMO-specific protease SENP7
    SUMO-1-specific protease 2

Gene names

Name:	SENP7
Synonyms:	KIAA1707, SSP2, SUSP2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	984 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Protease that deconjugates SUMO2 and SUMO3 from targeted proteins, but not SUMO1. Catalyzes the deconjugation of poly-SUMO2 and poly-SUMO3 chains. Has very low efficiency in processing full-length SUMO proteins to their mature forms. Ref.7
Sequence similarities	Belongs to the peptidase C48 family.

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Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein sumoylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	cysteine-type peptidase activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select] Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q9BQF6-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BQF6-2) The sequence of this isoform differs from the canonical sequence as follows: 1-33: Missing. 95-95: R → RQLKVMLTNVLWTDLGRKFRKTLPRNDANLCDANKVQSDSLPSTSVDSLETCQKLEPLRQSLNLSER

Isoform 3 (identifier: Q9BQF6-3) The sequence of this isoform differs from the canonical sequence as follows: 1-746: Missing. 747-761: TRKENNLTEDNPNLS → MKKFLYIKSVFHTLR

Isoform 4 (identifier: Q9BQF6-4) The sequence of this isoform differs from the canonical sequence as follows: 1-33: Missing. 94-158: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 984

984

Sentrin-specific protease 7

PRO_0000101726

Regions

Region

694 – 984

291

Protease

Sites

Active site

794

1

By similarity

Active site

926

1

By similarity

Amino acid modifications

Modified residue

377

1

Phosphoserine Ref.6

Modified residue

378

1

Phosphoserine Ref.6

Natural variations

Alternative sequence

1 – 746

746

Missing in isoform 3.

VSP_005277

Alternative sequence

1 – 33

33

Missing in isoform 2 and isoform 4.

VSP_005275

Alternative sequence

94 – 158

65

Missing in isoform 4.

VSP_021945

Alternative sequence

95

1

R → RQLKVMLTNVLWTDLGRKFR KTLPRNDANLCDANKVQSDS LPSTSVDSLETCQKLEPLRQ SLNLSER in isoform 2.

VSP_005276

Alternative sequence

747 – 761

15

TRKEN…NPNLS → MKKFLYIKSVFHTLR in isoform 3.

VSP_005278

Natural variant

79

1

K → Q: dbSNP rs6809436.

VAR_029651

Natural variant

546

1

Q → H: dbSNP rs2433031. Ref.5

VAR_029652

Experimental info

Mutagenesis

709

1

F → W: Slightly increased deconjugation activity. Ref.7

Mutagenesis

713

1

V → E: Reduces deconjugation activity. Ref.7

Sequence conflict

90

1

K → R in CAB66534. Ref.3

Sequence conflict

275

1

K → R in CAB66534. Ref.3

Sequence conflict

330

1

N → Y in CAB66534. Ref.3

Sequence conflict

719

1

L → H in AAL25651. Ref.1

Sequence conflict

828

1

N → S in CAB66534. Ref.3

Sequence conflict

881

1

Q → R in CAB66534. Ref.3

Sequence conflict

940

1

D → N in AAL25651. Ref.1

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified December 12, 2006. Version 3. Checksum: E5EBB74CAC3E39C5 Show »	FASTA	984	112,159
10 20 30 40 50 60 MDKRKLGRRP SSSEIITEGK RKKSSSDLSE IRKMLNAKPE DVHVQSPLSK FRSSERWTLP 70 80 90 100 110 120 LQWERSLRNK VISLDHKNKK HIRGCPVTSK SSPERIPRVI LTNVLGTELG RKYIRTPPVT 130 140 150 160 170 180 EGSLSDTDNL QSEQLSSSSD GSLESYQNLN PHKSCYLSER GSQRSKTVDD NSAKQTAHNK 190 200 210 220 230 240 EKRRKDDGIS LLISDTQPED LNSGSRGCDH LEQESRNKDV KYSDSKVELT LISRKTKRRL 250 260 270 280 290 300 RNNLPDSQYC TSLDKSTEQT KKQEDDSTIS TEFEKPSENY HQDPKLPEEI TTKPTKSDFT 310 320 330 340 350 360 KLSSLNSQEL TLSNATKSAS AGSTTETVEN SNSIDIVGIS SLVEKDENEL NTIEKPILRG 370 380 390 400 410 420 HNEGNQSLIS AEPIVVSSDE EGPVEHKSSE ILKLQSKQDR ETTNENESTS ESALLELPLI 430 440 450 460 470 480 TCESVQMSSE LCPYNPVMEN ISSIMPSNEM DLQLDFIFTS VYIGKIKGAS KGCVTITKKY 490 500 510 520 530 540 IKIPFQVSLN EISLLVDTTH LKRFGLWKSK DDNHSKRSHA ILFFWVSSDY LQEIQTQLEH 550 560 570 580 590 600 SVLSQQSKSS EFIFLELHNP VSQREELKLK DIMTEISIIS GELELSYPLS WVQAFPLFQN 610 620 630 640 650 660 LSSKESSFIH YYCVSTCSFP AGVAVAEEMK LKSVSQPSNT DAAKPTYTFL QKQSSGCYSL 670 680 690 700 710 720 SITSNPDEEW REVRHTGLVQ KLIVYPPPPT KGGLGVTNED LECLEEGEFL NDVIIDFYLK 730 740 750 760 770 780 YLILEKASDE LVERSHIFSS FFYKCLTRKE NNLTEDNPNL SMAQRRHKRV RTWTRHINIF 790 800 810 820 830 840 NKDYIFVPVN ESSHWYLAVI CFPWLEEAVY EDFPQTVSQQ SQAQQSQNDN KTIDNDLRTT 850 860 870 880 890 900 STLSLSAEDS QSTESNMSVP KKMCKRPCIL ILDSLKAASV QNTVQNLREY LEVEWEVKLK 910 920 930 940 950 960 THRQFSKTNM VDLCPKVPKQ DNSSDCGVYL LQYVESFFKD PIVNFELPIH LEKWFPRHVI 970 980 KTKREDIREL ILKLHLQQQK GSSS « Hide
Isoform 2. Checksum: F0C8278BCC440AA8 Show »	FASTA	1,017	115,871
10 20 30 40 50 60 MLNAKPEDVH VQSPLSKFRS SERWTLPLQW ERSLRNKVIS LDHKNKKHIR GCPVTSKSSP 70 80 90 100 110 120 ERQLKVMLTN VLWTDLGRKF RKTLPRNDAN LCDANKVQSD SLPSTSVDSL ETCQKLEPLR 130 140 150 160 170 180 QSLNLSERIP RVILTNVLGT ELGRKYIRTP PVTEGSLSDT DNLQSEQLSS SSDGSLESYQ 190 200 210 220 230 240 NLNPHKSCYL SERGSQRSKT VDDNSAKQTA HNKEKRRKDD GISLLISDTQ PEDLNSGSRG 250 260 270 280 290 300 CDHLEQESRN KDVKYSDSKV ELTLISRKTK RRLRNNLPDS QYCTSLDKST EQTKKQEDDS 310 320 330 340 350 360 TISTEFEKPS ENYHQDPKLP EEITTKPTKS DFTKLSSLNS QELTLSNATK SASAGSTTET 370 380 390 400 410 420 VENSNSIDIV GISSLVEKDE NELNTIEKPI LRGHNEGNQS LISAEPIVVS SDEEGPVEHK 430 440 450 460 470 480 SSEILKLQSK QDRETTNENE STSESALLEL PLITCESVQM SSELCPYNPV MENISSIMPS 490 500 510 520 530 540 NEMDLQLDFI FTSVYIGKIK GASKGCVTIT KKYIKIPFQV SLNEISLLVD TTHLKRFGLW 550 560 570 580 590 600 KSKDDNHSKR SHAILFFWVS SDYLQEIQTQ LEHSVLSQQS KSSEFIFLEL HNPVSQREEL 610 620 630 640 650 660 KLKDIMTEIS IISGELELSY PLSWVQAFPL FQNLSSKESS FIHYYCVSTC SFPAGVAVAE 670 680 690 700 710 720 EMKLKSVSQP SNTDAAKPTY TFLQKQSSGC YSLSITSNPD EEWREVRHTG LVQKLIVYPP 730 740 750 760 770 780 PPTKGGLGVT NEDLECLEEG EFLNDVIIDF YLKYLILEKA SDELVERSHI FSSFFYKCLT 790 800 810 820 830 840 RKENNLTEDN PNLSMAQRRH KRVRTWTRHI NIFNKDYIFV PVNESSHWYL AVICFPWLEE 850 860 870 880 890 900 AVYEDFPQTV SQQSQAQQSQ NDNKTIDNDL RTTSTLSLSA EDSQSTESNM SVPKKMCKRP 910 920 930 940 950 960 CILILDSLKA ASVQNTVQNL REYLEVEWEV KLKTHRQFSK TNMVDLCPKV PKQDNSSDCG 970 980 990 1000 1010 VYLLQYVESF FKDPIVNFEL PIHLEKWFPR HVIKTKREDI RELILKLHLQ QQKGSSS « Hide
Isoform 3. Checksum: 9661C4384D6A30D7 Show »	FASTA	238	28,124
10 20 30 40 50 60 MKKFLYIKSV FHTLRMAQRR HKRVRTWTRH INIFNKDYIF VPVNESSHWY LAVICFPWLE 70 80 90 100 110 120 EAVYEDFPQT VSQQSQAQQS QNDNKTIDND LRTTSTLSLS AEDSQSTESN MSVPKKMCKR 130 140 150 160 170 180 PCILILDSLK AASVQNTVQN LREYLEVEWE VKLKTHRQFS KTNMVDLCPK VPKQDNSSDC 190 200 210 220 230 GVYLLQYVES FFKDPIVNFE LPIHLEKWFP RHVIKTKRED IRELILKLHL QQQKGSSS « Hide
Isoform 4. Checksum: B03DA49EB00C0007 Show »	FASTA	886	101,195
10 20 30 40 50 60 MLNAKPEDVH VQSPLSKFRS SERWTLPLQW ERSLRNKVIS LDHKNKKHIR GCPVTSKSSP 70 80 90 100 110 120 ERGSQRSKTV DDNSAKQTAH NKEKRRKDDG ISLLISDTQP EDLNSGSRGC DHLEQESRNK 130 140 150 160 170 180 DVKYSDSKVE LTLISRKTKR RLRNNLPDSQ YCTSLDKSTE QTKKQEDDST ISTEFEKPSE 190 200 210 220 230 240 NYHQDPKLPE EITTKPTKSD FTKLSSLNSQ ELTLSNATKS ASAGSTTETV ENSNSIDIVG 250 260 270 280 290 300 ISSLVEKDEN ELNTIEKPIL RGHNEGNQSL ISAEPIVVSS DEEGPVEHKS SEILKLQSKQ 310 320 330 340 350 360 DRETTNENES TSESALLELP LITCESVQMS SELCPYNPVM ENISSIMPSN EMDLQLDFIF 370 380 390 400 410 420 TSVYIGKIKG ASKGCVTITK KYIKIPFQVS LNEISLLVDT THLKRFGLWK SKDDNHSKRS 430 440 450 460 470 480 HAILFFWVSS DYLQEIQTQL EHSVLSQQSK SSEFIFLELH NPVSQREELK LKDIMTEISI 490 500 510 520 530 540 ISGELELSYP LSWVQAFPLF QNLSSKESSF IHYYCVSTCS FPAGVAVAEE MKLKSVSQPS 550 560 570 580 590 600 NTDAAKPTYT FLQKQSSGCY SLSITSNPDE EWREVRHTGL VQKLIVYPPP PTKGGLGVTN 610 620 630 640 650 660 EDLECLEEGE FLNDVIIDFY LKYLILEKAS DELVERSHIF SSFFYKCLTR KENNLTEDNP 670 680 690 700 710 720 NLSMAQRRHK RVRTWTRHIN IFNKDYIFVP VNESSHWYLA VICFPWLEEA VYEDFPQTVS 730 740 750 760 770 780 QQSQAQQSQN DNKTIDNDLR TTSTLSLSAE DSQSTESNMS VPKKMCKRPC ILILDSLKAA 790 800 810 820 830 840 SVQNTVQNLR EYLEVEWEVK LKTHRQFSKT NMVDLCPKVP KQDNSSDCGV YLLQYVESFF 850 860 870 880 KDPIVNFELP IHLEKWFPRH VIKTKREDIR ELILKLHLQQ QKGSSS « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	Choi S.J., Jeon Y.-J., Kim K.I., Nishimori S., Suzuki T., Uchida S., Shimbara N., Tanaka K., Chung C.H. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"SENP7, a novel human sentrin-specific protease." Gong L., Yeh E.T.H. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Placenta.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Fetal kidney.
[5]	"Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O. DNA Res. 7:347-355(2000) [PubMed: 11214970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 229-984 (ISOFORM 1), VARIANT HIS-546. Tissue: Brain.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377 AND SER-378, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Structure of the human SENP7 catalytic domain and poly-SUMO deconjugation activities for SENP6 and SENP7." Lima C.D., Reverter D. J. Biol. Chem. 283:32045-32055(2008) [PubMed: 18799455] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 662-984, FUNCTION, MUTAGENESIS OF PHE-709 AND VAL-713.

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Cross-references

Sequence databases

AF199458 mRNA. Translation: AAL25651.1.
AF217504 mRNA. Translation: AAG09703.1.
AL136599 mRNA. Translation: CAB66534.1.
BX537943 mRNA. Translation: CAD97911.1.
AB051494 mRNA. Translation: BAB21798.1.

IPI

IPI00218776.
IPI00218777.
IPI00296449.
IPI00816671.

RefSeq

NP_001070671.1.
NP_065705.3.

UniGene

Hs.529551

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EAY	X-ray	2.40	A	662-984	[»]

ModBase

Search...

Protein family/group databases

MEROPS

C48.009.

PTM databases

PhosphoSite

Q9BQF6.

Genome annotation databases

Ensembl

ENSG00000138468. Homo sapiens. [Contig view]

GeneID

57337.

KEGG

hsa:57337.

NMPDR

fig|9606.3.peg.22897.

Organism-specific databases

GeneCards

GC03M102525.

H-InvDB

HIX0003507.

HGNC

HGNC:30402. SENP7.

PharmGKB

PA134925171.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q9BQF6.

Gene expression databases

ArrayExpress

Q9BQF6.

Bgee

Q9BQF6.

CleanEx

HS_SENP7.

GermOnline

ENSG00000138468. Homo sapiens.

Family and domain databases

InterPro

IPR003653. Peptidase_C48.
[Graphical view]

Pfam

PF02902. Peptidase_C48. 1 hit.
[Graphical view]

PROSITE

PS50600. ULP_PROTEASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

63454.

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Entry information

Entry name

SENP7_HUMAN

Accession

Primary (citable) accession number: Q9BQF6
Secondary accession number(s): Q7Z3F4

Q9HBT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 63 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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