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Protein

Diphthamide biosynthesis protein 2

Gene

DPH2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).By similarity

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthamide biosynthesis protein 2
Alternative name(s):
DPH2 homolog
Short name:
HsDph2
Diphthamide biosynthesis protein 2 homolog-like 2
Short name:
DPH2-like 2
Gene namesi
Name:DPH2
Synonyms:DPH2L2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:3004. DPH2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi1802.
OpenTargetsiENSG00000132768.
PharmGKBiPA27462.

Polymorphism and mutation databases

BioMutaiDPH2.
DMDMi74761201.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003078891 – 489Diphthamide biosynthesis protein 2Add BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei435PhosphothreonineCombined sources1
Modified residuei446PhosphoserineCombined sources1
Modified residuei456PhosphoserineCombined sources1
Modified residuei467PhosphothreonineCombined sources1
Modified residuei488PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BQC3.
MaxQBiQ9BQC3.
PaxDbiQ9BQC3.
PeptideAtlasiQ9BQC3.
PRIDEiQ9BQC3.

PTM databases

iPTMnetiQ9BQC3.
PhosphoSitePlusiQ9BQC3.

Expressioni

Tissue specificityi

Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes.1 Publication

Gene expression databases

BgeeiENSG00000132768.
CleanExiHS_DPH2.
ExpressionAtlasiQ9BQC3. baseline and differential.
GenevisibleiQ9BQC3. HS.

Organism-specific databases

HPAiHPA045796.

Interactioni

Subunit structurei

Interacts with DPH1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
IL17AQ165523EBI-10237931,EBI-10237926

Protein-protein interaction databases

BioGridi108136. 21 interactors.
IntActiQ9BQC3. 3 interactors.
MINTiMINT-2815901.
STRINGi9606.ENSP00000255108.

Structurei

3D structure databases

ProteinModelPortaliQ9BQC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DPH1/DPH2 family. DPH2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2648. Eukaryota.
COG1736. LUCA.
GeneTreeiENSGT00550000075105.
HOGENOMiHOG000015311.
HOVERGENiHBG107823.
InParanoidiQ9BQC3.
KOiK17866.
OMAiCCPDTGQ.
OrthoDBiEOG091G09K9.
PhylomeDBiQ9BQC3.
TreeFamiTF313832.

Family and domain databases

InterProiIPR010014. DHP2_eu.
IPR016435. DPH1/DPH2.
[Graphical view]
PANTHERiPTHR10762. PTHR10762. 1 hit.
PfamiPF01866. Diphthamide_syn. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00322. diphth2_R. 1 hit.
TIGR00272. DPH2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BQC3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESMFSSPAE AALQRETGVP GLLTPLPDLD GVYELERVAG FVRDLGCERV
60 70 80 90 100
ALQFPDQLLG DAVAVAARLE ETTGSKMFIL GDTAYGSCCV DVLGAEQAGA
110 120 130 140 150
QALIHFGPAC LSPPARPLPV AFVLRQRSVA LELCVKAFEA QNPDPKAPVV
160 170 180 190 200
LLSEPACAHA LEALATLLRP RYLDLLVSSP AFPQPVGSLS PEPMPLERFG
210 220 230 240 250
RRFPLAPGRR LEEYGAFYVG GSKASPDPDL DPDLSRLLLG WAPGQPFSSC
260 270 280 290 300
CPDTGKTQDE GARAGRLRAR RRYLVERARD ARVVGLLAGT LGVAQHREAL
310 320 330 340 350
AHLRNLTQAA GKRSYVLALG RPTPAKLANF PEVDVFVLLA CPLGALAPQL
360 370 380 390 400
SGSFFQPILA PCELEAACNP AWPPPGLAPH LTHYADLLPG SPFHVALPPP
410 420 430 440 450
ESELWETPDV SLITGDLRPP PAWKSSNDHG SLALTPRPQL ELAESSPAAS
460 470 480
FLSSRSWQGL EPRLGQTPVT EAVSGRRGIA IAYEDEGSG
Length:489
Mass (Da):52,083
Last modified:June 1, 2001 - v1
Checksum:i2908FB7E816E3AEF
GO
Isoform 2 (identifier: Q9BQC3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     162-389: Missing.

Note: Gene prediction based on EST data.
Show »
Length:261
Mass (Da):27,529
Checksum:iCC4F41D6B2CE16BA
GO
Isoform 3 (identifier: Q9BQC3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MESMFSSPAEAALQRETGVPGLLTPL → MLWLWLHDWRRRQGQRCSFWVTQPTA
     27-161: Missing.

Note: No experimental confirmation available.
Show »
Length:354
Mass (Da):38,510
Checksum:iBD0FBC0456CAC76F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125 – 153RQRSV…VVLLS → SSTFCGLGTLCQDLWGPKPR PQSACGAAG in AAC18086 (PubMed:9782084).CuratedAdd BLAST29
Sequence conflicti266R → G in AAC18086 (PubMed:9782084).Curated1
Sequence conflicti435T → N in AAC18086 (PubMed:9782084).Curated1
Sequence conflicti449A → V in AAC18086 (PubMed:9782084).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0560521 – 26MESMF…LLTPL → MLWLWLHDWRRRQGQRCSFW VTQPTA in isoform 3. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_05605327 – 161Missing in isoform 3. 1 PublicationAdd BLAST135
Alternative sequenceiVSP_047151162 – 389Missing in isoform 2. CuratedAdd BLAST228

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053003 mRNA. Translation: AAC18086.1.
AK297933 mRNA. Translation: BAG60250.1.
AK315506 mRNA. Translation: BAG37890.1.
BT007431 mRNA. Translation: AAP36099.1.
AL357079 Genomic DNA. Translation: CAI16800.1.
CH471059 Genomic DNA. Translation: EAX07070.1.
BC001389 mRNA. Translation: AAH01389.1.
BC003181 mRNA. Translation: AAH03181.1.
BC016956 mRNA. Translation: AAH16956.1.
CCDSiCCDS41314.1. [Q9BQC3-2]
CCDS504.1. [Q9BQC3-1]
RefSeqiNP_001034678.1. NM_001039589.1. [Q9BQC3-2]
NP_001306095.1. NM_001319166.1.
NP_001306098.1. NM_001319169.1. [Q9BQC3-3]
NP_001375.2. NM_001384.4. [Q9BQC3-1]
UniGeneiHs.632398.

Genome annotation databases

EnsembliENST00000255108; ENSP00000255108; ENSG00000132768. [Q9BQC3-1]
ENST00000396758; ENSP00000379981; ENSG00000132768. [Q9BQC3-2]
GeneIDi1802.
KEGGihsa:1802.
UCSCiuc001ckz.4. human. [Q9BQC3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053003 mRNA. Translation: AAC18086.1.
AK297933 mRNA. Translation: BAG60250.1.
AK315506 mRNA. Translation: BAG37890.1.
BT007431 mRNA. Translation: AAP36099.1.
AL357079 Genomic DNA. Translation: CAI16800.1.
CH471059 Genomic DNA. Translation: EAX07070.1.
BC001389 mRNA. Translation: AAH01389.1.
BC003181 mRNA. Translation: AAH03181.1.
BC016956 mRNA. Translation: AAH16956.1.
CCDSiCCDS41314.1. [Q9BQC3-2]
CCDS504.1. [Q9BQC3-1]
RefSeqiNP_001034678.1. NM_001039589.1. [Q9BQC3-2]
NP_001306095.1. NM_001319166.1.
NP_001306098.1. NM_001319169.1. [Q9BQC3-3]
NP_001375.2. NM_001384.4. [Q9BQC3-1]
UniGeneiHs.632398.

3D structure databases

ProteinModelPortaliQ9BQC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108136. 21 interactors.
IntActiQ9BQC3. 3 interactors.
MINTiMINT-2815901.
STRINGi9606.ENSP00000255108.

PTM databases

iPTMnetiQ9BQC3.
PhosphoSitePlusiQ9BQC3.

Polymorphism and mutation databases

BioMutaiDPH2.
DMDMi74761201.

Proteomic databases

EPDiQ9BQC3.
MaxQBiQ9BQC3.
PaxDbiQ9BQC3.
PeptideAtlasiQ9BQC3.
PRIDEiQ9BQC3.

Protocols and materials databases

DNASUi1802.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000255108; ENSP00000255108; ENSG00000132768. [Q9BQC3-1]
ENST00000396758; ENSP00000379981; ENSG00000132768. [Q9BQC3-2]
GeneIDi1802.
KEGGihsa:1802.
UCSCiuc001ckz.4. human. [Q9BQC3-1]

Organism-specific databases

CTDi1802.
DisGeNETi1802.
GeneCardsiDPH2.
HGNCiHGNC:3004. DPH2.
HPAiHPA045796.
MIMi603456. gene.
neXtProtiNX_Q9BQC3.
OpenTargetsiENSG00000132768.
PharmGKBiPA27462.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2648. Eukaryota.
COG1736. LUCA.
GeneTreeiENSGT00550000075105.
HOGENOMiHOG000015311.
HOVERGENiHBG107823.
InParanoidiQ9BQC3.
KOiK17866.
OMAiCCPDTGQ.
OrthoDBiEOG091G09K9.
PhylomeDBiQ9BQC3.
TreeFamiTF313832.

Enzyme and pathway databases

UniPathwayiUPA00559.
ReactomeiR-HSA-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

ChiTaRSiDPH2. human.
GenomeRNAii1802.
PROiQ9BQC3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132768.
CleanExiHS_DPH2.
ExpressionAtlasiQ9BQC3. baseline and differential.
GenevisibleiQ9BQC3. HS.

Family and domain databases

InterProiIPR010014. DHP2_eu.
IPR016435. DPH1/DPH2.
[Graphical view]
PANTHERiPTHR10762. PTHR10762. 1 hit.
PfamiPF01866. Diphthamide_syn. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00322. diphth2_R. 1 hit.
TIGR00272. DPH2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPH2_HUMAN
AccessioniPrimary (citable) accession number: Q9BQC3
Secondary accession number(s): A8MVC9
, B2RDE3, B4DNI8, O60623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.