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Protein

Methylosome protein 50

Gene

WDR77

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.2 Publications

GO - Molecular functioni

  • ligand-dependent nuclear receptor transcription coactivator activity Source: MGI
  • methyl-CpG binding Source: UniProtKB
  • protein-arginine N-methyltransferase activity Source: Reactome

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-191859. snRNP Assembly.
R-HSA-3214858. RMTs methylate histone arginines.
SignaLinkiQ9BQA1.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylosome protein 50
Short name:
MEP-50
Alternative name(s):
Androgen receptor cofactor p44
WD repeat-containing protein 77
p44/Mep50
Gene namesi
Name:WDR77
Synonyms:MEP50, WD45
ORF Names:HKMT1069, Nbla10071
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:29652. WDR77.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • Golgi apparatus Source: HPA
  • methylosome Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi79084.
OpenTargetsiENSG00000116455.
PharmGKBiPA142670581.

Chemistry databases

ChEMBLiCHEMBL3137261.

Polymorphism and mutation databases

BioMutaiWDR77.
DMDMi32171507.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510741 – 342Methylosome protein 50Add BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphothreonineCombined sources1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BQA1.
MaxQBiQ9BQA1.
PaxDbiQ9BQA1.
PeptideAtlasiQ9BQA1.
PRIDEiQ9BQA1.

2D gel databases

REPRODUCTION-2DPAGEIPI00012202.

PTM databases

iPTMnetiQ9BQA1.
PhosphoSitePlusiQ9BQA1.

Expressioni

Tissue specificityi

Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.2 Publications

Developmental stagei

Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.1 Publication

Gene expression databases

BgeeiENSG00000116455.
CleanExiHS_WDR77.
ExpressionAtlasiQ9BQA1. baseline and differential.
GenevisibleiQ9BQA1. HS.

Organism-specific databases

HPAiHPA026437.
HPA026448.
HPA027271.

Interactioni

Subunit structurei

Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1A, WDR77, PRMT1 and ERH (PubMed:25284789). Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a compact hetero-octamer with PRMT5, decorating the outer surface of a PRMT5 tetramer. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts with APEX1, AR and NKX3-1. Interacts with CHTOP (PubMed:25284789).9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NP034183EBI-1237307,EBI-6930799From a different organism.
PRMT5O147447EBI-1237307,EBI-351098

Protein-protein interaction databases

BioGridi122532. 115 interactors.
DIPiDIP-38172N.
IntActiQ9BQA1. 56 interactors.
MINTiMINT-1217135.
STRINGi9606.ENSP00000235090.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 36Combined sources8
Beta strandi42 – 47Combined sources6
Beta strandi50 – 52Combined sources3
Beta strandi56 – 63Combined sources8
Helixi64 – 66Combined sources3
Helixi70 – 72Combined sources3
Beta strandi74 – 81Combined sources8
Beta strandi83 – 89Combined sources7
Turni90 – 92Combined sources3
Beta strandi93 – 98Combined sources6
Beta strandi101 – 108Combined sources8
Beta strandi110 – 113Combined sources4
Beta strandi115 – 122Combined sources8
Beta strandi128 – 133Combined sources6
Beta strandi137 – 144Combined sources8
Beta strandi149 – 153Combined sources5
Turni154 – 157Combined sources4
Beta strandi158 – 163Combined sources6
Beta strandi170 – 175Combined sources6
Beta strandi182 – 187Combined sources6
Beta strandi192 – 196Combined sources5
Beta strandi199 – 201Combined sources3
Beta strandi203 – 205Combined sources3
Beta strandi208 – 211Combined sources4
Beta strandi215 – 220Combined sources6
Beta strandi222 – 224Combined sources3
Beta strandi227 – 232Combined sources6
Beta strandi235 – 242Combined sources8
Beta strandi249 – 252Combined sources4
Beta strandi258 – 263Combined sources6
Beta strandi265 – 268Combined sources4
Beta strandi271 – 275Combined sources5
Beta strandi280 – 283Combined sources4
Beta strandi289 – 293Combined sources5
Beta strandi300 – 305Combined sources6
Beta strandi307 – 309Combined sources3
Beta strandi312 – 317Combined sources6
Beta strandi322 – 326Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GQBX-ray2.06B2-342[»]
4X60X-ray2.35B2-342[»]
4X61X-ray2.85B2-342[»]
4X63X-ray3.05B2-342[»]
5C9ZX-ray2.36B2-342[»]
5EMJX-ray2.27B2-342[»]
5EMKX-ray2.52B2-342[»]
5EMLX-ray2.39B2-342[»]
5EMMX-ray2.37B2-342[»]
5FA5X-ray2.34B2-342[»]
ProteinModelPortaliQ9BQA1.
SMRiQ9BQA1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati22 – 75WD 1PROSITE-ProRule annotation1 PublicationAdd BLAST54
Repeati78 – 116WD 2PROSITE-ProRule annotation1 PublicationAdd BLAST39
Repeati123 – 162WD 3PROSITE-ProRule annotation1 PublicationAdd BLAST40
Repeati165 – 205WD 4PROSITE-ProRule annotation1 PublicationAdd BLAST41
Repeati209 – 250WD 5PROSITE-ProRule annotation1 PublicationAdd BLAST42
Repeati253 – 293WD 6PROSITE-ProRule annotation1 PublicationAdd BLAST41
Repeati295 – 330WD 7PROSITE-ProRule annotation1 PublicationAdd BLAST36

Sequence similaritiesi

Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IP8S. Eukaryota.
ENOG4111F0R. LUCA.
GeneTreeiENSGT00390000010711.
HOGENOMiHOG000035094.
HOVERGENiHBG052458.
InParanoidiQ9BQA1.
KOiK13221.
OMAiSGSKDIC.
OrthoDBiEOG091G0T6F.
PhylomeDBiQ9BQA1.
TreeFamiTF325967.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BQA1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKETPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG ALLLGASSLS
60 70 80 90 100
GRCWAGSLWL FKDPCAAPNE GFCSAGVQTE AGVADLTWVG ERGILVASDS
110 120 130 140 150
GAVELWELDE NETLIVSKFC KYEHDDIVST VSVLSSGTQA VSGSKDICIK
160 170 180 190 200
VWDLAQQVVL SSYRAHAAQV TCVAASPHKD SVFLSCSEDN RILLWDTRCP
210 220 230 240 250
KPASQIGCSA PGYLPTSLAW HPQQSEVFVF GDENGTVSLV DTKSTSCVLS
260 270 280 290 300
SAVHSQCVTG LVFSPHSVPF LASLSEDCSL AVLDSSLSEL FRSQAHRDFV
310 320 330 340
RDATWSPLNH SLLTTVGWDH QVVHHVVPTE PLPAPGPASV TE
Length:342
Mass (Da):36,724
Last modified:June 1, 2001 - v1
Checksum:i3D355AEC68491ECB
GO
Isoform 2 (identifier: Q9BQA1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     101-164: Missing.

Note: No experimental confirmation available.
Show »
Length:278
Mass (Da):29,651
Checksum:i1A8FE679CD05CB24
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244S → N in BAD96909 (PubMed:12972618).Curated1
Sequence conflicti313 – 342LTTVG…ASVTE → DLQVLLSRLDLRQKASPP in AAH09411 (PubMed:15489334).CuratedAdd BLAST30

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04290348S → I.Corresponds to variant rs7416672dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056166101 – 164Missing in isoform 2. 1 PublicationAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF478464 mRNA. Translation: AAL79917.1.
AK022860 mRNA. Translation: BAG51128.1.
AK298179 mRNA. Translation: BAG60450.1.
AK223189 mRNA. Translation: BAD96909.1.
AY225316 mRNA. Translation: AAP79114.1.
AB073603 mRNA. Translation: BAD38641.1.
AL390195 Genomic DNA. Translation: CAC36041.1.
CH471122 Genomic DNA. Translation: EAW56493.1.
BC001679 mRNA. Translation: AAH01679.1.
BC006477 mRNA. Translation: AAH06477.1.
BC009411 mRNA. Translation: AAH09411.1.
BC011778 mRNA. Translation: AAH11778.1.
BC016946 mRNA. Translation: AAH16946.1.
AB074171 mRNA. Translation: BAE45736.1.
CCDSiCCDS835.1. [Q9BQA1-1]
RefSeqiNP_001303991.1. NM_001317062.1.
NP_001303992.1. NM_001317063.1.
NP_001303993.1. NM_001317064.1. [Q9BQA1-2]
NP_077007.1. NM_024102.3. [Q9BQA1-1]
UniGeneiHs.204773.

Genome annotation databases

EnsembliENST00000235090; ENSP00000235090; ENSG00000116455. [Q9BQA1-1]
GeneIDi79084.
KEGGihsa:79084.
UCSCiuc001ebb.4. human. [Q9BQA1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF478464 mRNA. Translation: AAL79917.1.
AK022860 mRNA. Translation: BAG51128.1.
AK298179 mRNA. Translation: BAG60450.1.
AK223189 mRNA. Translation: BAD96909.1.
AY225316 mRNA. Translation: AAP79114.1.
AB073603 mRNA. Translation: BAD38641.1.
AL390195 Genomic DNA. Translation: CAC36041.1.
CH471122 Genomic DNA. Translation: EAW56493.1.
BC001679 mRNA. Translation: AAH01679.1.
BC006477 mRNA. Translation: AAH06477.1.
BC009411 mRNA. Translation: AAH09411.1.
BC011778 mRNA. Translation: AAH11778.1.
BC016946 mRNA. Translation: AAH16946.1.
AB074171 mRNA. Translation: BAE45736.1.
CCDSiCCDS835.1. [Q9BQA1-1]
RefSeqiNP_001303991.1. NM_001317062.1.
NP_001303992.1. NM_001317063.1.
NP_001303993.1. NM_001317064.1. [Q9BQA1-2]
NP_077007.1. NM_024102.3. [Q9BQA1-1]
UniGeneiHs.204773.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GQBX-ray2.06B2-342[»]
4X60X-ray2.35B2-342[»]
4X61X-ray2.85B2-342[»]
4X63X-ray3.05B2-342[»]
5C9ZX-ray2.36B2-342[»]
5EMJX-ray2.27B2-342[»]
5EMKX-ray2.52B2-342[»]
5EMLX-ray2.39B2-342[»]
5EMMX-ray2.37B2-342[»]
5FA5X-ray2.34B2-342[»]
ProteinModelPortaliQ9BQA1.
SMRiQ9BQA1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122532. 115 interactors.
DIPiDIP-38172N.
IntActiQ9BQA1. 56 interactors.
MINTiMINT-1217135.
STRINGi9606.ENSP00000235090.

Chemistry databases

ChEMBLiCHEMBL3137261.

PTM databases

iPTMnetiQ9BQA1.
PhosphoSitePlusiQ9BQA1.

Polymorphism and mutation databases

BioMutaiWDR77.
DMDMi32171507.

2D gel databases

REPRODUCTION-2DPAGEIPI00012202.

Proteomic databases

EPDiQ9BQA1.
MaxQBiQ9BQA1.
PaxDbiQ9BQA1.
PeptideAtlasiQ9BQA1.
PRIDEiQ9BQA1.

Protocols and materials databases

DNASUi79084.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000235090; ENSP00000235090; ENSG00000116455. [Q9BQA1-1]
GeneIDi79084.
KEGGihsa:79084.
UCSCiuc001ebb.4. human. [Q9BQA1-1]

Organism-specific databases

CTDi79084.
DisGeNETi79084.
GeneCardsiWDR77.
HGNCiHGNC:29652. WDR77.
HPAiHPA026437.
HPA026448.
HPA027271.
MIMi611734. gene.
neXtProtiNX_Q9BQA1.
OpenTargetsiENSG00000116455.
PharmGKBiPA142670581.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IP8S. Eukaryota.
ENOG4111F0R. LUCA.
GeneTreeiENSGT00390000010711.
HOGENOMiHOG000035094.
HOVERGENiHBG052458.
InParanoidiQ9BQA1.
KOiK13221.
OMAiSGSKDIC.
OrthoDBiEOG091G0T6F.
PhylomeDBiQ9BQA1.
TreeFamiTF325967.

Enzyme and pathway databases

ReactomeiR-HSA-191859. snRNP Assembly.
R-HSA-3214858. RMTs methylate histone arginines.
SignaLinkiQ9BQA1.

Miscellaneous databases

ChiTaRSiWDR77. human.
GeneWikiiWD_repeat-containing_protein_77.
GenomeRNAii79084.
PROiQ9BQA1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116455.
CleanExiHS_WDR77.
ExpressionAtlasiQ9BQA1. baseline and differential.
GenevisibleiQ9BQA1. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEP50_HUMAN
AccessioniPrimary (citable) accession number: Q9BQA1
Secondary accession number(s): B3KMW6
, B4DP38, Q3LID2, Q53FU2, Q6JZZ5, Q96GK4, Q9BWY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.