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Protein

Structure-specific endonuclease subunit SLX1

Gene

SLX1A

more
Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products.UniRule annotation3 Publications

Caution

Found in a segmental duplication on p arm of chromosome 16 giving rise to two identical copies of this gene sharing exons with SULT1A3 and SULT1A4.Curated

Cofactori

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri186 – 238SLX1-typeUniRule annotationAdd BLAST53

GO - Molecular functioni

GO - Biological processi

  • DNA double-strand break processing involved in repair via single-strand annealing Source: UniProtKB
  • DNA repair Source: UniProtKB
  • double-strand break repair via homologous recombination Source: UniProtKB
  • interstrand cross-link repair Source: Reactome
  • negative regulation of telomere maintenance via telomere lengthening Source: BHF-UCL
  • positive regulation of t-circle formation Source: BHF-UCL
  • t-circle formation Source: BHF-UCL
  • telomere maintenance via telomere lengthening Source: BHF-UCL
  • telomeric D-loop disassembly Source: BHF-UCL

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-HSA-6783310 Fanconi Anemia Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Structure-specific endonuclease subunit SLX1UniRule annotation (EC:3.1.-.-UniRule annotation)
Alternative name(s):
GIY-YIG domain-containing protein 1UniRule annotation
Gene namesi
Name:SLX1AUniRule annotation
Synonyms:GIYD1UniRule annotation, SLX1
AND
Name:SLX1BUniRule annotation
Synonyms:GIYD2, SLX1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000132207.17
HostDB:ENSG00000181625.17
HGNCiHGNC:20922 SLX1A
HGNC:28748 SLX1B
MIMi615822 gene
615823 gene
neXtProtiNX_Q9BQ83

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41R → A: Abolishes endonucleolytic activity. 1 Publication1
Mutagenesisi82E → A: Abolishes endonucleolytic activity. 2 Publications1

Organism-specific databases

OpenTargetsiENSG00000132207
PharmGKBiPA142671738

Polymorphism and mutation databases

BioMutaiSLX1A
DMDMi74732820

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003321201 – 275Structure-specific endonuclease subunit SLX1Add BLAST275

Proteomic databases

PaxDbiQ9BQ83
PeptideAtlasiQ9BQ83
PRIDEiQ9BQ83
ProteomicsDBi78640
78641 [Q9BQ83-2]

PTM databases

iPTMnetiQ9BQ83
PhosphoSitePlusiQ9BQ83

Expressioni

Gene expression databases

BgeeiENSG00000132207
ExpressionAtlasiQ9BQ83 baseline and differential
GenevisibleiQ9BQ83 HS

Organism-specific databases

HPAiHPA047038

Interactioni

Subunit structurei

Forms a heterodimer with SLX4.

Binary interactionsi

WithEntry#Exp.IntActNotes
SLX4Q8IY9210EBI-2370858,EBI-2370740

Protein-protein interaction databases

BioGridi122479, 43 interactors
139225, 27 interactors
IntActiQ9BQ83, 55 interactors
STRINGi9606.ENSP00000328940

Structurei

3D structure databases

ProteinModelPortaliQ9BQ83
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 95GIY-YIGUniRule annotationAdd BLAST84

Sequence similaritiesi

Belongs to the SLX1 family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri186 – 238SLX1-typeUniRule annotationAdd BLAST53

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3005 Eukaryota
ENOG411266C LUCA
GeneTreeiENSGT00390000013368
HOGENOMiHOG000008012
InParanoidiQ9BQ83
KOiK15078
OMAiCHMLCLA
OrthoDBiEOG091G0J09
PhylomeDBiQ9BQ83
TreeFamiTF352344

Family and domain databases

Gene3Di3.30.40.10, 1 hit
3.40.1440.10, 1 hit
HAMAPiMF_03100 Endonuc_su_Slx1, 1 hit
InterProiView protein in InterPro
IPR000305 GIY-YIG_endonuc
IPR035901 GIY-YIG_endonuc_sf
IPR027520 Slx1
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01541 GIY-YIG, 1 hit
SUPFAMiSSF82771 SSF82771, 1 hit
PROSITEiView protein in PROSITE
PS50164 GIY_YIG, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BQ83-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPAGVAARP GRFFGVYLLY CLNPRYRGRV YVGFTVNTAR RVQQHNGGRK
60 70 80 90 100
KGGAWRTSGR GPWEMVLVVH GFPSSVAALR FEWAWQHPHA SRRLAHVGPR
110 120 130 140 150
LRGETAFAFH LRVLAHMLRA PPWARLPLTL RWVRPDLRQD LCLPPPPHVP
160 170 180 190 200
LAFGPPPPQA PAPRRRAGPF DDAEPEPDQG DPGACCSLCA QTIQDEEGPL
210 220 230 240 250
CCPHPGCLLR AHVICLAEEF LQEEPGQLLP LEGQCPCCEK SLLWGDLIWL
260 270
CQMDTEKEVE DSELEEAHWT DLLET
Length:275
Mass (Da):30,771
Last modified:June 1, 2001 - v1
Checksum:i57DF055F2E59CF70
GO
Isoform 2 (identifier: Q9BQ83-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-194: Missing.

Show »
Length:161
Mass (Da):18,012
Checksum:i4182E39967F1FDE9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03333181 – 194Missing in isoform 2. 1 PublicationAdd BLAST114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC106782 Genomic DNA No translation available.
AC133555 Genomic DNA No translation available.
BC000754 mRNA Translation: AAH00754.1
BC000803 mRNA Translation: AAH00803.1
BC015990 mRNA Translation: AAH15990.1
BC019306 mRNA Translation: AAH19306.1
BC069007 mRNA Translation: AAH69007.1
BC130545 mRNA Translation: AAI30546.1
BC130547 mRNA Translation: AAI30548.1
BC144462 mRNA Translation: AAI44463.1
CCDSiCCDS10648.1 [Q9BQ83-1]
CCDS10649.1 [Q9BQ83-2]
CCDS32431.1 [Q9BQ83-1]
CCDS32432.1 [Q9BQ83-2]
RefSeqiNP_001014999.1, NM_001014999.2 [Q9BQ83-1]
NP_001015000.1, NM_001015000.2 [Q9BQ83-2]
NP_076949.1, NM_024044.3 [Q9BQ83-1]
NP_835145.1, NM_178044.2 [Q9BQ83-2]
UniGeneiHs.728161
Hs.729791

Genome annotation databases

EnsembliENST00000251303; ENSP00000251303; ENSG00000132207 [Q9BQ83-1]
ENST00000330181; ENSP00000328940; ENSG00000181625 [Q9BQ83-1]
ENST00000345535; ENSP00000333945; ENSG00000132207 [Q9BQ83-2]
ENST00000351581; ENSP00000335316; ENSG00000181625 [Q9BQ83-2]
GeneIDi548593
79008
KEGGihsa:548593
hsa:79008
UCSCiuc002dsx.4 human [Q9BQ83-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSLX1_HUMAN
AccessioniPrimary (citable) accession number: Q9BQ83
Secondary accession number(s): B7ZME1, Q6NTG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2001
Last modified: June 20, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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