Three prime repair exonuclease 2 - Homo sapiens (Human)

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Protein attributes

General annotation (Comments)

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Reviewed, UniProtKB/Swiss-Prot Q9BQ50 (TREX2_HUMAN)

Last modified November 3, 2009. Version 66. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Protein names

Recommended name:
    Three prime repair exonuclease 2
    EC=3.1.11.2
Alternative name(s):
    3'-5' exonuclease TREX2

Gene names

Name:

TREX2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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Function	Exonuclease with a preference for double stranded DNA with mismatched 3' termini. May play a role in DNA repair. Ref.4
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Cofactor	Magnesium. Required for activity. Substitution with Mn²⁺ results in partial activity.
Subunit structure	Homodimer. Ref.4
Subcellular location	Nucleus Probable.
Tissue specificity	Detected in heart, breast, prostate, skeletal muscle, testis, uterus, bone marrow, colon, small intestine, stomach and thymus. Ref.3
Sequence similarities	Belongs to the exonuclease superfamily. TREX family.
Caution	The gene for this protein is either identical to or adjacent to that of UCHL5IP. Most mRNAs that encode UCHL5IP also include the N-terminal part of TREX2.
Biophysicochemical properties	pH dependence: Optimum pH is 7.5-8.0.

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Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Magnesium
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exodeoxyribonuclease activity Ref.1 Traceable author statement. Source: ProtInc exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 279

279

Three prime repair exonuclease 2

PRO_0000109870

Alternative sequence

1 – 43

Missing in isoform 2.

VSP_010447

Natural variant

180

R → C

VAR_025211

279

Helix Strand Turn

Details...

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Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 91387E045960C1EA Show »	FASTA	279	30,621
10 20 30 40 50 60 MGRAGSPLPR SSWPRMDDCG SRSRCSPTLC SSLRTCYPRG NITMSEAPRA ETFVFLDLEA 70 80 90 100 110 120 TGLPSVEPEI AELSLFAVHR SSLENPEHDE SGALVLPRVL DKLTLCMCPE RPFTAKASEI 130 140 150 160 170 180 TGLSSEGLAR CRKAGFDGAV VRTLQAFLSR QAGPICLVAH NGFDYDFPLL CAELRRLGAR 190 200 210 220 230 240 LPRDTVCLDT LPALRGLDRA HSHGTRARGR QGYSLGSLFH RYFRAEPSAA HSAEGDVHTL 250 260 270 LLIFLHRAAE LLAWADEQAR GWAHIEPMYL PPDDPSLEA « Hide
Isoform 2. Checksum: 3363BE40CFCC2300 Show »	FASTA	236	25,922
10 20 30 40 50 60 MSEAPRAETF VFLDLEATGL PSVEPEIAEL SLFAVHRSSL ENPEHDESGA LVLPRVLDKL 70 80 90 100 110 120 TLCMCPERPF TAKASEITGL SSEGLARCRK AGFDGAVVRT LQAFLSRQAG PICLVAHNGF 130 140 150 160 170 180 DYDFPLLCAE LRRLGARLPR DTVCLDTLPA LRGLDRAHSH GTRARGRQGY SLGSLFHRYF 190 200 210 220 230 RAEPSAAHSA EGDVHTLLLI FLHRAAELLA WADEQARGWA HIEPMYLPPD DPSLEA « Hide

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[1]	"Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3'-->5' exonucleases." Mazur D.J., Perrino F.W. J. Biol. Chem. 274:19655-19660(1999) [PubMed: 10391904] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]	NIEHS SNPs program Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT CYS-180.
[3]	"Structure and expression of the TREX1 and TREX2 3'-->5' exonuclease genes." Mazur D.J., Perrino F.W. J. Biol. Chem. 276:14718-14727(2001) [PubMed: 11278605] [Abstract] Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[4]	"Excision of 3' termini by the Trex1 and TREX2 3'-->5' exonucleases. Characterization of the recombinant proteins." Mazur D.J., Perrino F.W. J. Biol. Chem. 276:17022-17029(2001) [PubMed: 11279105] [Abstract] Cited for: FUNCTION, CHARACTERIZATION, HOMODIMERIZATION.
[5]	"The human TREX2 3' ->5'-exonuclease structure suggests a mechanism for efficient nonprocessive DNA catalysis." Perrino F.W., Harvey S., McMillin S., Hollis T. J. Biol. Chem. 280:15212-15218(2005) [PubMed: 15661738] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 44-279.
+	Additional computationally mapped references.

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NIEHS-SNPs

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AF151107 mRNA. Translation: AAD48776.1.
AF319570 mRNA. Translation: AAK07617.1.
AF319571 mRNA. Translation: AAK07618.1.
AF319572 mRNA. Translation: AAK07619.1.
AF319573 mRNA. Translation: AAK07620.1.
DQ145722 Genomic DNA. Translation: AAZ38719.1.

IPI

IPI00383247.
IPI00939474.

RefSeq

NP_542432.2.

UniGene

Hs.644635

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y97	X-ray	2.50	A/B	44-279	[»]

ModBase

Search...

STRING

Q9BQ50.

PRIDE

Q9BQ50.

Ensembl

GeneID

11219.

KEGG

hsa:11219.

UCSC

uc004fhj.1. human.

CTD

11219.

GeneCards

GC0XM152363.

H-InvDB

HIX0017129.

HGNC

HGNC:12270. TREX2.

MIM

300370. gene.

PharmGKB

PA36950.

GenAtlas

Search...

HOVERGEN

Q9BQ50.

OMA

AETFVFL.

BRENDA

3.1.11.2. 247.

ArrayExpress

Q9BQ50.

Bgee

Q9BQ50.

CleanEx

HS_TREX2.

Genevestigator

Q9BQ50.

GermOnline

ENSG00000183479. Homo sapiens.

InterPro

IPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
[Graphical view]

Pfam

PF00929. Exonuc_X-T. 1 hit.
[Graphical view]

SMART

SM00479. EXOIII. 1 hit.
[Graphical view]

ProtoNet

Search...

NextBio

42703.

SOURCE

Search...

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This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BQ50-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BQ50-2)

The sequence of this isoform differs from the canonical sequence as follows:
1-43: Missing.

Entry name

TREX2_HUMAN

Accession

Primary (citable) accession number: Q9BQ50
Secondary accession number(s): Q45F08, Q9UN77

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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