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Protein

Three prime repair exonuclease 2

Gene

TREX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exonuclease with a preference for double-stranded DNA with mismatched 3' termini. May play a role in DNA repair.1 Publication

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ per subunit. The second magnesium ion interacts with only one residue. Substitution with Mn2+ results in partial activity.By similarity

pH dependencei

Optimum pH is 7.5-8.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57Magnesium 1By similarity1
Metal bindingi57Magnesium 2By similarity1
Metal bindingi59Magnesium 1By similarity1
Binding sitei165SubstrateBy similarity1
Active sitei231Proton donor/acceptorBy similarity1
Metal bindingi236Magnesium 1By similarity1
Binding sitei236SubstrateBy similarity1

GO - Molecular functioni

  • 3'-5'-exodeoxyribonuclease activity Source: UniProtKB
  • exodeoxyribonuclease III activity Source: UniProtKB-EC
  • magnesium ion binding Source: UniProtKB
  • nucleic acid binding Source: InterPro
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • DNA metabolic process Source: UniProtKB
  • DNA repair Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:HS00004-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Three prime repair exonuclease 2 (EC:3.1.11.2)
Alternative name(s):
3'-5' exonuclease TREX2
Gene namesi
Name:TREX2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:12270. TREX2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57D → A: Loss of enzyme activity; when associated with A-59. Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi59E → A: Loss of enzyme activity; when associated with A-57. Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi166D → A: Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi206R → A: Strongly reduces DNA-binding; when associated with A-208 and A-210. 1 Publication1
Mutagenesisi208R → A: Strongly reduces DNA-binding; when associated with A-206 and A-210. 1 Publication1
Mutagenesisi210R → A: Strongly reduces DNA-binding; when associated with A-208 and A-208. 1 Publication1
Mutagenesisi231H → A: Loss of enzyme activity; when associated with A-236. Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi236D → A: Loss of enzyme activity; when associated with A-231. Almost abolishes enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi11219.
OpenTargetsiENSG00000183479.
PharmGKBiPA36950.

Polymorphism and mutation databases

BioMutaiTREX2.
DMDMi47606206.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098701 – 279Three prime repair exonuclease 2Add BLAST279

Proteomic databases

PaxDbiQ9BQ50.
PeptideAtlasiQ9BQ50.
PRIDEiQ9BQ50.

PTM databases

PhosphoSitePlusiQ9BQ50.

Expressioni

Tissue specificityi

Detected in heart, breast, prostate, skeletal muscle, testis, uterus, bone marrow, colon, small intestine, stomach and thymus.1 Publication

Gene expression databases

BgeeiENSG00000183479.
CleanExiHS_TREX2.
GenevisibleiQ9BQ50. HS.

Organism-specific databases

HPAiHPA054060.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi116388. 10 interactors.
MINTiMINT-1440359.
STRINGi9606.ENSP00000333441.

Structurei

Secondary structure

1279
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi51 – 62Combined sources12
Helixi64 – 66Combined sources3
Beta strandi70 – 79Combined sources10
Helixi80 – 83Combined sources4
Beta strandi101 – 106Combined sources6
Helixi115 – 121Combined sources7
Helixi125 – 130Combined sources6
Helixi138 – 148Combined sources11
Beta strandi153 – 159Combined sources7
Turni160 – 165Combined sources6
Helixi166 – 176Combined sources11
Beta strandi186 – 189Combined sources4
Helixi190 – 200Combined sources11
Helixi215 – 223Combined sources9
Helixi233 – 246Combined sources14
Helixi248 – 258Combined sources11
Helixi262 – 264Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y97X-ray2.50A/B44-279[»]
ProteinModelPortaliQ9BQ50.
SMRiQ9BQ50.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BQ50.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 60Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the exonuclease superfamily. TREX family.Curated

Phylogenomic databases

eggNOGiKOG4793. Eukaryota.
ENOG4111YSP. LUCA.
GeneTreeiENSGT00390000012715.
HOGENOMiHOG000118119.
HOVERGENiHBG079278.
InParanoidiQ9BQ50.
KOiK10791.
OMAiDVHTLLM.
OrthoDBiEOG091G0LSM.
PhylomeDBiQ9BQ50.
TreeFamiTF323333.

Family and domain databases

Gene3Di3.30.420.10. 2 hits.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BQ50-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRAGSPLPR SSWPRMDDCG SRSRCSPTLC SSLRTCYPRG NITMSEAPRA
60 70 80 90 100
ETFVFLDLEA TGLPSVEPEI AELSLFAVHR SSLENPEHDE SGALVLPRVL
110 120 130 140 150
DKLTLCMCPE RPFTAKASEI TGLSSEGLAR CRKAGFDGAV VRTLQAFLSR
160 170 180 190 200
QAGPICLVAH NGFDYDFPLL CAELRRLGAR LPRDTVCLDT LPALRGLDRA
210 220 230 240 250
HSHGTRARGR QGYSLGSLFH RYFRAEPSAA HSAEGDVHTL LLIFLHRAAE
260 270
LLAWADEQAR GWAHIEPMYL PPDDPSLEA
Length:279
Mass (Da):30,621
Last modified:June 1, 2001 - v1
Checksum:i91387E045960C1EA
GO
Isoform 2 (identifier: Q9BQ50-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Show »
Length:236
Mass (Da):25,922
Checksum:i3363BE40CFCC2300
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025211180R → C.1 PublicationCorresponds to variant rs35132777dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0104471 – 43Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151107 mRNA. Translation: AAD48776.1.
AF319570 mRNA. Translation: AAK07617.1.
AF319571 mRNA. Translation: AAK07618.1.
AF319572 mRNA. Translation: AAK07619.1.
AF319573 mRNA. Translation: AAK07620.1.
DQ145722 Genomic DNA. Translation: AAZ38719.1.
CCDSiCCDS35437.1. [Q9BQ50-2]
RefSeqiNP_542432.2. NM_080701.3. [Q9BQ50-2]
UniGeneiHs.644635.

Genome annotation databases

EnsembliENST00000330912; ENSP00000333441; ENSG00000183479. [Q9BQ50-2]
ENST00000334497; ENSP00000334993; ENSG00000183479. [Q9BQ50-1]
ENST00000338525; ENSP00000345218; ENSG00000183479. [Q9BQ50-2]
ENST00000370212; ENSP00000359231; ENSG00000183479. [Q9BQ50-1]
ENST00000370231; ENSP00000359251; ENSG00000183479. [Q9BQ50-2]
ENST00000370232; ENSP00000359252; ENSG00000183479. [Q9BQ50-1]
ENST00000393862; ENSP00000377442; ENSG00000183479. [Q9BQ50-2]
GeneIDi11219.
KEGGihsa:11219.
UCSCiuc011myp.2. human. [Q9BQ50-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151107 mRNA. Translation: AAD48776.1.
AF319570 mRNA. Translation: AAK07617.1.
AF319571 mRNA. Translation: AAK07618.1.
AF319572 mRNA. Translation: AAK07619.1.
AF319573 mRNA. Translation: AAK07620.1.
DQ145722 Genomic DNA. Translation: AAZ38719.1.
CCDSiCCDS35437.1. [Q9BQ50-2]
RefSeqiNP_542432.2. NM_080701.3. [Q9BQ50-2]
UniGeneiHs.644635.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y97X-ray2.50A/B44-279[»]
ProteinModelPortaliQ9BQ50.
SMRiQ9BQ50.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116388. 10 interactors.
MINTiMINT-1440359.
STRINGi9606.ENSP00000333441.

PTM databases

PhosphoSitePlusiQ9BQ50.

Polymorphism and mutation databases

BioMutaiTREX2.
DMDMi47606206.

Proteomic databases

PaxDbiQ9BQ50.
PeptideAtlasiQ9BQ50.
PRIDEiQ9BQ50.

Protocols and materials databases

DNASUi11219.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330912; ENSP00000333441; ENSG00000183479. [Q9BQ50-2]
ENST00000334497; ENSP00000334993; ENSG00000183479. [Q9BQ50-1]
ENST00000338525; ENSP00000345218; ENSG00000183479. [Q9BQ50-2]
ENST00000370212; ENSP00000359231; ENSG00000183479. [Q9BQ50-1]
ENST00000370231; ENSP00000359251; ENSG00000183479. [Q9BQ50-2]
ENST00000370232; ENSP00000359252; ENSG00000183479. [Q9BQ50-1]
ENST00000393862; ENSP00000377442; ENSG00000183479. [Q9BQ50-2]
GeneIDi11219.
KEGGihsa:11219.
UCSCiuc011myp.2. human. [Q9BQ50-1]

Organism-specific databases

CTDi11219.
DisGeNETi11219.
GeneCardsiTREX2.
H-InvDBHIX0017129.
HGNCiHGNC:12270. TREX2.
HPAiHPA054060.
MIMi300370. gene.
neXtProtiNX_Q9BQ50.
OpenTargetsiENSG00000183479.
PharmGKBiPA36950.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4793. Eukaryota.
ENOG4111YSP. LUCA.
GeneTreeiENSGT00390000012715.
HOGENOMiHOG000118119.
HOVERGENiHBG079278.
InParanoidiQ9BQ50.
KOiK10791.
OMAiDVHTLLM.
OrthoDBiEOG091G0LSM.
PhylomeDBiQ9BQ50.
TreeFamiTF323333.

Enzyme and pathway databases

BioCyciZFISH:HS00004-MONOMER.

Miscellaneous databases

ChiTaRSiTREX2. human.
EvolutionaryTraceiQ9BQ50.
GeneWikiiTREX2.
GenomeRNAii11219.
PROiQ9BQ50.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000183479.
CleanExiHS_TREX2.
GenevisibleiQ9BQ50. HS.

Family and domain databases

Gene3Di3.30.420.10. 2 hits.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTREX2_HUMAN
AccessioniPrimary (citable) accession number: Q9BQ50
Secondary accession number(s): Q45F08, Q9UN77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

The gene for this protein is either identical to or adjacent to that of UCHL5IP. Most mRNAs that encode UCHL5IP also include the N-terminal part of TREX2.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.