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Protein

ATP-dependent RNA helicase DDX50

Gene

DDX50

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi181 – 188ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000107625-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX50 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 50
Gu-beta
Nucleolar protein Gu2
Gene namesi
Name:DDX50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:17906. DDX50.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • nucleolus Source: HPA
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi79009.
OpenTargetsiENSG00000107625.
PharmGKBiPA134948525.

Polymorphism and mutation databases

BioMutaiDDX50.
DMDMi55976580.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550541 – 737ATP-dependent RNA helicase DDX50Add BLAST737

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineCombined sources1
Modified residuei82PhosphoserineCombined sources1
Modified residuei86PhosphoserineCombined sources1
Modified residuei121PhosphoserineBy similarity1
Modified residuei122PhosphoserineBy similarity1
Modified residuei247PhosphothreonineBy similarity1
Modified residuei518PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BQ39.
MaxQBiQ9BQ39.
PaxDbiQ9BQ39.
PeptideAtlasiQ9BQ39.
PRIDEiQ9BQ39.

2D gel databases

SWISS-2DPAGEQ9BQ39.

PTM databases

iPTMnetiQ9BQ39.
PhosphoSitePlusiQ9BQ39.
SwissPalmiQ9BQ39.

Expressioni

Gene expression databases

BgeeiENSG00000107625.
CleanExiHS_DDX50.
ExpressionAtlasiQ9BQ39. baseline and differential.
GenevisibleiQ9BQ39. HS.

Organism-specific databases

HPAiHPA037388.
HPA037389.

Interactioni

Subunit structurei

Interacts with C1QBP.1 Publication

Protein-protein interaction databases

BioGridi122480. 33 interactors.
IntActiQ9BQ39. 17 interactors.
MINTiMINT-4727989.
STRINGi9606.ENSP00000362687.

Structurei

Secondary structure

1737
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi587 – 593Combined sources7
Helixi602 – 611Combined sources10
Helixi614 – 617Combined sources4
Beta strandi621 – 625Combined sources5
Beta strandi629 – 637Combined sources9
Helixi638 – 647Combined sources10
Beta strandi650 – 652Combined sources3
Beta strandi654 – 656Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E29NMR-A575-659[»]
ProteinModelPortaliQ9BQ39.
SMRiQ9BQ39.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BQ39.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 347Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST180
Domaini380 – 524Helicase C-terminalPROSITE-ProRule annotationAdd BLAST145

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 165Q motifAdd BLAST29
Motifi290 – 293DEVD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi677 – 728Arg-richAdd BLAST52

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00820000126976.
HOGENOMiHOG000268805.
HOVERGENiHBG051331.
InParanoidiQ9BQ39.
KOiK13183.
OMAiCEKKSKR.
OrthoDBiEOG091G04F8.
PhylomeDBiQ9BQ39.
TreeFamiTF328622.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR012562. GUCT.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF08152. GUCT. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BQ39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGKLLWGDI MELEAPLEES ESQKKERQKS DRRKSRHHYD SDEKSETREN
60 70 80 90 100
GVTDDLDAPK AKKSKMKEKL NGDTEEGFNR LSDEFSKSHK SRRKDLPNGD
110 120 130 140 150
IDEYEKKSKR VSSLDTSTHK SSDNKLEETL TREQKEGAFS NFPISEETIK
160 170 180 190 200
LLKGRGVTYL FPIQVKTFGP VYEGKDLIAQ ARTGTGKTFS FAIPLIERLQ
210 220 230 240 250
RNQETIKKSR SPKVLVLAPT RELANQVAKD FKDITRKLSV ACFYGGTSYQ
260 270 280 290 300
SQINHIRNGI DILVGTPGRI KDHLQSGRLD LSKLRHVVLD EVDQMLDLGF
310 320 330 340 350
AEQVEDIIHE SYKTDSEDNP QTLLFSATCP QWVYKVAKKY MKSRYEQVDL
360 370 380 390 400
VGKMTQKAAT TVEHLAIQCH WSQRPAVIGD VLQVYSGSEG RAIIFCETKK
410 420 430 440 450
NVTEMAMNPH IKQNAQCLHG DIAQSQREIT LKGFREGSFK VLVATNVAAR
460 470 480 490 500
GLDIPEVDLV IQSSPPQDVE SYIHRSGRTG RAGRTGICIC FYQPRERGQL
510 520 530 540 550
RYVEQKAGIT FKRVGVPSTM DLVKSKSMDA IRSLASVSYA AVDFFRPSAQ
560 570 580 590 600
RLIEEKGAVD ALAAALAHIS GASSFEPRSL ITSDKGFVTM TLESLEEIQD
610 620 630 640 650
VSCAWKELNR KLSSNAVSQI TRMCLLKGNM GVCFDVPTTE SERLQAEWHD
660 670 680 690 700
SDWILSVPAK LPEIEEYYDG NTSSNSRQRS GWSSGRSGRS GRSGGRSGGR
710 720 730
SGRQSRQGSR SGSRQDGRRR SGNRNRSRSG GHKRSFD
Length:737
Mass (Da):82,565
Last modified:June 1, 2001 - v1
Checksum:i9B7EF72EEC7C504E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti680S → I in AAH18637 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334103 mRNA. Translation: AAK29402.1.
AL359844 Genomic DNA. Translation: CAH72376.1.
CH471083 Genomic DNA. Translation: EAW54304.1.
BC000210 mRNA. Translation: AAH00210.1.
BC000272 mRNA. Translation: AAH00272.1.
BC018637 mRNA. Translation: AAH18637.2.
CCDSiCCDS7283.1.
RefSeqiNP_076950.1. NM_024045.1.
XP_016872115.1. XM_017016626.1.
UniGeneiHs.522984.

Genome annotation databases

EnsembliENST00000373585; ENSP00000362687; ENSG00000107625.
GeneIDi79009.
KEGGihsa:79009.
UCSCiuc001jou.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334103 mRNA. Translation: AAK29402.1.
AL359844 Genomic DNA. Translation: CAH72376.1.
CH471083 Genomic DNA. Translation: EAW54304.1.
BC000210 mRNA. Translation: AAH00210.1.
BC000272 mRNA. Translation: AAH00272.1.
BC018637 mRNA. Translation: AAH18637.2.
CCDSiCCDS7283.1.
RefSeqiNP_076950.1. NM_024045.1.
XP_016872115.1. XM_017016626.1.
UniGeneiHs.522984.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E29NMR-A575-659[»]
ProteinModelPortaliQ9BQ39.
SMRiQ9BQ39.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122480. 33 interactors.
IntActiQ9BQ39. 17 interactors.
MINTiMINT-4727989.
STRINGi9606.ENSP00000362687.

PTM databases

iPTMnetiQ9BQ39.
PhosphoSitePlusiQ9BQ39.
SwissPalmiQ9BQ39.

Polymorphism and mutation databases

BioMutaiDDX50.
DMDMi55976580.

2D gel databases

SWISS-2DPAGEQ9BQ39.

Proteomic databases

EPDiQ9BQ39.
MaxQBiQ9BQ39.
PaxDbiQ9BQ39.
PeptideAtlasiQ9BQ39.
PRIDEiQ9BQ39.

Protocols and materials databases

DNASUi79009.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373585; ENSP00000362687; ENSG00000107625.
GeneIDi79009.
KEGGihsa:79009.
UCSCiuc001jou.3. human.

Organism-specific databases

CTDi79009.
DisGeNETi79009.
GeneCardsiDDX50.
HGNCiHGNC:17906. DDX50.
HPAiHPA037388.
HPA037389.
MIMi610373. gene.
neXtProtiNX_Q9BQ39.
OpenTargetsiENSG00000107625.
PharmGKBiPA134948525.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00820000126976.
HOGENOMiHOG000268805.
HOVERGENiHBG051331.
InParanoidiQ9BQ39.
KOiK13183.
OMAiCEKKSKR.
OrthoDBiEOG091G04F8.
PhylomeDBiQ9BQ39.
TreeFamiTF328622.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000107625-MONOMER.

Miscellaneous databases

ChiTaRSiDDX50. human.
EvolutionaryTraceiQ9BQ39.
GeneWikiiDDX50.
GenomeRNAii79009.
PROiQ9BQ39.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107625.
CleanExiHS_DDX50.
ExpressionAtlasiQ9BQ39. baseline and differential.
GenevisibleiQ9BQ39. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR012562. GUCT.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF08152. GUCT. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX50_HUMAN
AccessioniPrimary (citable) accession number: Q9BQ39
Secondary accession number(s): Q5VX37, Q8WV76, Q9BWI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.