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Q9BQ39 (DDX50_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent RNA helicase DDX50

EC=3.6.4.13
Alternative name(s):
DEAD box protein 50
Gu-beta
Nucleolar protein Gu2
Gene names
Name:DDX50
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length737 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with C1QBP. Ref.9

Subcellular location

Nucleusnucleolus Ref.5.

Sequence similarities

Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 737737ATP-dependent RNA helicase DDX50
PRO_0000055054

Regions

Domain168 – 347180Helicase ATP-binding
Domain380 – 524145Helicase C-terminal
Nucleotide binding181 – 1888ATP By similarity
Motif137 – 16529Q motif
Motif290 – 2934DEVD box
Compositional bias677 – 72852Arg-rich

Amino acid modifications

Modified residue411Phosphoserine Ref.10
Modified residue821Phosphoserine Ref.6 Ref.7
Modified residue861Phosphoserine Ref.7

Experimental info

Sequence conflict6801S → I in AAH18637. Ref.4

Secondary structure

................ 737
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9BQ39 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 9B7EF72EEC7C504E

FASTA73782,565
        10         20         30         40         50         60 
MPGKLLWGDI MELEAPLEES ESQKKERQKS DRRKSRHHYD SDEKSETREN GVTDDLDAPK 

        70         80         90        100        110        120 
AKKSKMKEKL NGDTEEGFNR LSDEFSKSHK SRRKDLPNGD IDEYEKKSKR VSSLDTSTHK 

       130        140        150        160        170        180 
SSDNKLEETL TREQKEGAFS NFPISEETIK LLKGRGVTYL FPIQVKTFGP VYEGKDLIAQ 

       190        200        210        220        230        240 
ARTGTGKTFS FAIPLIERLQ RNQETIKKSR SPKVLVLAPT RELANQVAKD FKDITRKLSV 

       250        260        270        280        290        300 
ACFYGGTSYQ SQINHIRNGI DILVGTPGRI KDHLQSGRLD LSKLRHVVLD EVDQMLDLGF 

       310        320        330        340        350        360 
AEQVEDIIHE SYKTDSEDNP QTLLFSATCP QWVYKVAKKY MKSRYEQVDL VGKMTQKAAT 

       370        380        390        400        410        420 
TVEHLAIQCH WSQRPAVIGD VLQVYSGSEG RAIIFCETKK NVTEMAMNPH IKQNAQCLHG 

       430        440        450        460        470        480 
DIAQSQREIT LKGFREGSFK VLVATNVAAR GLDIPEVDLV IQSSPPQDVE SYIHRSGRTG 

       490        500        510        520        530        540 
RAGRTGICIC FYQPRERGQL RYVEQKAGIT FKRVGVPSTM DLVKSKSMDA IRSLASVSYA 

       550        560        570        580        590        600 
AVDFFRPSAQ RLIEEKGAVD ALAAALAHIS GASSFEPRSL ITSDKGFVTM TLESLEEIQD 

       610        620        630        640        650        660 
VSCAWKELNR KLSSNAVSQI TRMCLLKGNM GVCFDVPTTE SERLQAEWHD SDWILSVPAK 

       670        680        690        700        710        720 
LPEIEEYYDG NTSSNSRQRS GWSSGRSGRS GRSGGRSGGR SGRQSRQGSR SGSRQDGRRR 

       730 
SGNRNRSRSG GHKRSFD 

« Hide

References

« Hide 'large scale' references
[1]"Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAs."
Valdez B.C., Yang H., Hong E., Sequitin A.M.
Gene 284:53-61(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye and Skin.
[5]"Functional proteomic analysis of human nucleolus."
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82 AND SER-86, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Splicing factor 2-associated protein p32 participates in ribosome biogenesis by regulating the binding of Nop52 and fibrillarin to preribosome particles."
Yoshikawa H., Komatsu W., Hayano T., Miura Y., Homma K., Izumikawa K., Ishikawa H., Miyazawa N., Tachikawa H., Yamauchi Y., Isobe T., Takahashi N.
Mol. Cell. Proteomics 10:0-0(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH C1QBP.
[10]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Solution structure of the GUCT domain from human RNA helicase II/Gu beta reveals the RRM fold, but implausible RNA interactions."
Ohnishi S., Paakkonen K., Koshiba S., Tochio N., Sato M., Kobayashi N., Harada T., Watanabe S., Muto Y., Guntert P., Tanaka A., Kigawa T., Yokoyama S.
Proteins 74:133-144(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 570-659.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF334103 mRNA. Translation: AAK29402.1.
AL359844 Genomic DNA. Translation: CAH72376.1.
CH471083 Genomic DNA. Translation: EAW54304.1.
BC000210 mRNA. Translation: AAH00210.1.
BC000272 mRNA. Translation: AAH00272.1.
BC018637 mRNA. Translation: AAH18637.2.
CCDSCCDS7283.1.
RefSeqNP_076950.1. NM_024045.1.
UniGeneHs.522984.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E29NMR-A575-659[»]
ProteinModelPortalQ9BQ39.
SMRQ9BQ39. Positions 123-659.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122480. 15 interactions.
IntActQ9BQ39. 9 interactions.
MINTMINT-4727989.
STRING9606.ENSP00000362687.

PTM databases

PhosphoSiteQ9BQ39.

Polymorphism databases

DMDM55976580.

2D gel databases

SWISS-2DPAGEQ9BQ39.

Proteomic databases

MaxQBQ9BQ39.
PaxDbQ9BQ39.
PeptideAtlasQ9BQ39.
PRIDEQ9BQ39.

Protocols and materials databases

DNASU79009.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000373585; ENSP00000362687; ENSG00000107625.
GeneID79009.
KEGGhsa:79009.
UCSCuc001jou.3. human.

Organism-specific databases

CTD79009.
GeneCardsGC10P070661.
HGNCHGNC:17906. DDX50.
HPAHPA037388.
MIM610373. gene.
neXtProtNX_Q9BQ39.
PharmGKBPA134948525.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0513.
HOGENOMHOG000268805.
HOVERGENHBG051331.
InParanoidQ9BQ39.
KOK13183.
OMANSHKSSD.
OrthoDBEOG77HDD6.
PhylomeDBQ9BQ39.
TreeFamTF328622.

Gene expression databases

BgeeQ9BQ39.
CleanExHS_DDX50.
GenevestigatorQ9BQ39.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR012562. GUCT.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF08152. GUCT. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDDX50. human.
EvolutionaryTraceQ9BQ39.
GeneWikiDDX50.
GenomeRNAi79009.
NextBio67657.
PROQ9BQ39.
SOURCESearch...

Entry information

Entry nameDDX50_HUMAN
AccessionPrimary (citable) accession number: Q9BQ39
Secondary accession number(s): Q5VX37, Q8WV76, Q9BWI8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM