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Protein

Potassium voltage-gated channel subfamily S member 3

Gene

KCNS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1; modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1 (PubMed:10484328). Heterotetrameric channel activity formed with KCNB1 show increased current amplitude with the threshold for action potential activation shifted towards more negative values in hypoxic-treated pulmonary artery smooth muscle cells (By similarity).By similarity1 Publication

GO - Molecular functioni

  • delayed rectifier potassium channel activity Source: ProtInc
  • potassium channel regulator activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_75770. Voltage gated Potassium channels.

Protein family/group databases

TCDBi1.A.1.2.15. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily S member 3
Alternative name(s):
Delayed-rectifier K(+) channel alpha subunit 3
Voltage-gated potassium channel subunit Kv9.3
Gene namesi
Name:KCNS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:6302. KCNS3.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein Curated

  • Note: May not reach the plasma membrane but remain in an intracellular compartment in the absence of KCNB1 (PubMed:10484328).1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 182182CytoplasmicBy similarityAdd
BLAST
Transmembranei183 – 20422Helical; Name=Segment S1By similarityAdd
BLAST
Topological domaini205 – 22016ExtracellularBy similarityAdd
BLAST
Transmembranei221 – 24323Helical; Name=Segment S2By similarityAdd
BLAST
Topological domaini244 – 25411CytoplasmicBy similarityAdd
BLAST
Transmembranei255 – 27521Helical; Name=Segment S3By similarityAdd
BLAST
Topological domaini276 – 28510ExtracellularBy similarity
Transmembranei286 – 30621Helical; Voltage-sensor; Name=Segment S4By similarityAdd
BLAST
Topological domaini307 – 32115CytoplasmicBy similarityAdd
BLAST
Transmembranei322 – 34322Helical; Name=Segment S5By similarityAdd
BLAST
Topological domaini344 – 35714ExtracellularBy similarityAdd
BLAST
Intramembranei358 – 36912Helical; Name=Pore helixBy similarityAdd
BLAST
Intramembranei370 – 3778By similarity
Topological domaini378 – 3847ExtracellularBy similarity
Transmembranei385 – 41329Helical; Name=Segment S6By similarityAdd
BLAST
Topological domaini414 – 49178CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30080.

Polymorphism and mutation databases

BioMutaiKCNS3.
DMDMi311033434.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Potassium voltage-gated channel subfamily S member 3PRO_0000054087Add
BLAST

Proteomic databases

PaxDbiQ9BQ31.
PRIDEiQ9BQ31.

Expressioni

Tissue specificityi

Detected in whole normal term placental and placental chorionic plate arteries and veins. Detected in syncytiotrophoblast and in blood vessel endothelium within intermediate villi and chorionic plate (at protein level) (PubMed:22943705). Detected in most tissues, but not in peripheral blood lymphocytes. The highest levels of expression are in lung (PubMed:10484328).2 Publications

Gene expression databases

BgeeiQ9BQ31.
CleanExiHS_KCNS3.
ExpressionAtlasiQ9BQ31. baseline and differential.
GenevisibleiQ9BQ31. HS.

Organism-specific databases

HPAiHPA014864.

Interactioni

Subunit structurei

Heterotetramer with KCNB1 (PubMed:10484328). Does not form homomultimers (PubMed:10484328).1 Publication

Protein-protein interaction databases

BioGridi109991. 2 interactions.
IntActiQ9BQ31. 1 interaction.
STRINGi9606.ENSP00000305824.

Structurei

3D structure databases

ProteinModelPortaliQ9BQ31.
SMRiQ9BQ31. Positions 14-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi370 – 3756Selectivity filterBy similarity

Domaini

The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118981.
HOGENOMiHOG000231016.
HOVERGENiHBG052230.
InParanoidiQ9BQ31.
KOiK04933.
OMAiVCWWWAT.
OrthoDBiEOG7CRTPP.
PhylomeDBiQ9BQ31.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BQ31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFGEFFHRP GQDEELVNLN VGGFKQSVDQ STLLRFPHTR LGKLLTCHSE
60 70 80 90 100
EAILELCDDY SVADKEYYFD RNPSLFRYVL NFYYTGKLHV MEELCVFSFC
110 120 130 140 150
QEIEYWGINE LFIDSCCSNR YQERKEENHE KDWDQKSHDV STDSSFEESS
160 170 180 190 200
LFEKELEKFD TLRFGQLRKK IWIRMENPAY CLSAKLIAIS SLSVVLASIV
210 220 230 240 250
AMCVHSMSEF QNEDGEVDDP VLEGVEIACI AWFTGELAVR LAAAPCQKKF
260 270 280 290 300
WKNPLNIIDF VSIIPFYATL AVDTKEEESE DIENMGKVVQ ILRLMRIFRI
310 320 330 340 350
LKLARHSVGL RSLGATLRHS YHEVGLLLLF LSVGISIFSV LIYSVEKDDH
360 370 380 390 400
TSSLTSIPIC WWWATISMTT VGYGDTHPVT LAGKLIASTC IICGILVVAL
410 420 430 440 450
PITIIFNKFS KYYQKQKDID VDQCSEDAPE KCHELPYFNI RDIYAQRMHT
460 470 480 490
FITSLSSVGI VVSDPDSTDA SSIEDNEDIC NTTSLENCTA K
Length:491
Mass (Da):56,001
Last modified:November 2, 2010 - v3
Checksum:iFFE02CA3DCA50185
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271S → Y in AAH15947 (PubMed:15815621).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti225 – 2251V → L.1 Publication
Corresponds to variant rs17856097 [ dbSNP | Ensembl ].
VAR_036986
Natural varianti450 – 4501T → A.3 Publications
Corresponds to variant rs4832524 [ dbSNP | Ensembl ].
VAR_014200

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043472 mRNA. Translation: AAC13164.1.
AK314451 mRNA. Translation: BAG37059.1.
AC093731 Genomic DNA. Translation: AAX88969.1.
CH471053 Genomic DNA. Translation: EAX00861.1.
CH471053 Genomic DNA. Translation: EAX00862.1.
BC004148 mRNA. Translation: AAH04148.1.
BC004987 mRNA. Translation: AAH04987.1.
BC015947 mRNA. Translation: AAH15947.1.
CCDSiCCDS1692.1.
RefSeqiNP_001269357.1. NM_001282428.1.
NP_002243.3. NM_002252.4.
XP_011531127.1. XM_011532825.1.
UniGeneiHs.414489.

Genome annotation databases

EnsembliENST00000304101; ENSP00000305824; ENSG00000170745.
ENST00000403915; ENSP00000385968; ENSG00000170745.
GeneIDi3790.
KEGGihsa:3790.
UCSCiuc002rcv.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043472 mRNA. Translation: AAC13164.1.
AK314451 mRNA. Translation: BAG37059.1.
AC093731 Genomic DNA. Translation: AAX88969.1.
CH471053 Genomic DNA. Translation: EAX00861.1.
CH471053 Genomic DNA. Translation: EAX00862.1.
BC004148 mRNA. Translation: AAH04148.1.
BC004987 mRNA. Translation: AAH04987.1.
BC015947 mRNA. Translation: AAH15947.1.
CCDSiCCDS1692.1.
RefSeqiNP_001269357.1. NM_001282428.1.
NP_002243.3. NM_002252.4.
XP_011531127.1. XM_011532825.1.
UniGeneiHs.414489.

3D structure databases

ProteinModelPortaliQ9BQ31.
SMRiQ9BQ31. Positions 14-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109991. 2 interactions.
IntActiQ9BQ31. 1 interaction.
STRINGi9606.ENSP00000305824.

Chemistry

ChEMBLiCHEMBL2362996.

Protein family/group databases

TCDBi1.A.1.2.15. the voltage-gated ion channel (vic) superfamily.

Polymorphism and mutation databases

BioMutaiKCNS3.
DMDMi311033434.

Proteomic databases

PaxDbiQ9BQ31.
PRIDEiQ9BQ31.

Protocols and materials databases

DNASUi3790.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304101; ENSP00000305824; ENSG00000170745.
ENST00000403915; ENSP00000385968; ENSG00000170745.
GeneIDi3790.
KEGGihsa:3790.
UCSCiuc002rcv.3. human.

Organism-specific databases

CTDi3790.
GeneCardsiGC02P018059.
H-InvDBHIX0001848.
HGNCiHGNC:6302. KCNS3.
HPAiHPA014864.
MIMi603888. gene.
neXtProtiNX_Q9BQ31.
PharmGKBiPA30080.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118981.
HOGENOMiHOG000231016.
HOVERGENiHBG052230.
InParanoidiQ9BQ31.
KOiK04933.
OMAiVCWWWAT.
OrthoDBiEOG7CRTPP.
PhylomeDBiQ9BQ31.
TreeFamiTF313103.

Enzyme and pathway databases

ReactomeiREACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_75770. Voltage gated Potassium channels.

Miscellaneous databases

GeneWikiiKCNS3.
GenomeRNAii3790.
NextBioi14883.
PROiQ9BQ31.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BQ31.
CleanExiHS_KCNS3.
ExpressionAtlasiQ9BQ31. baseline and differential.
GenevisibleiQ9BQ31. HS.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003971. K_chnl_volt-dep_Kv9.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01494. KV9CHANNEL.
PR01491. KVCHANNEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Electrically silent potassium channel subunits from human lens epithelium."
    Shepard A.R., Rae J.L.
    Am. J. Physiol. 277:C412-C424(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, VARIANT ALA-450.
    Tissue: Lens epithelium.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-450.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-225 AND ALA-450.
    Tissue: Kidney and Skin.
  6. "Expression of an electrically silent voltage-gated potassium channel in the human placenta."
    Fyfe G.K., Panicker S., Jones R.L., Wareing M.
    J. Obstet. Gynaecol. 32:624-629(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiKCNS3_HUMAN
AccessioniPrimary (citable) accession number: Q9BQ31
Secondary accession number(s): D6W520
, O43651, Q4ZFY1, Q96B56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: November 2, 2010
Last modified: July 22, 2015
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.