Q9BQ16 (TICN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testican-3

Alternative name(s):
SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 3

Gene names

Name:	SPOCK3
Synonyms:	TICN3
ORF Names:	UNQ409/PRO771

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May participate in diverse steps of neurogenesis. Inhibits the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. May interfere with tumor invasion.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Expressed in brain.
Post-translational modification	Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides By similarity.
Sequence similarities	Contains 1 Kazal-like domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
Ligand	Calcium
Molecular function	Metalloenzyme inhibitor Metalloprotease inhibitor
PTM	Disulfide bond Glycoprotein Heparan sulfate Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular matrix Inferred from direct assay Ref.2. Source: UniProtKB extracellular space Inferred from direct assay Ref.2. Source: UniProtKB proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro metalloendopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3 (identifier: Q9BQ16-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q9BQ16-1)

The sequence of this isoform differs from the canonical sequence as follows:
     64-66: Missing.

Isoform 2 (identifier: Q9BQ16-2)

Also known as: N-tes;

The sequence of this isoform differs from the canonical sequence as follows:
     314-316: DPP → GKR
     317-436: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 436	415	Testican-3		PRO_0000026703
Regions
	Domain	133 – 185	53	Kazal-like
	Domain	314 – 380	67	Thyroglobulin type-1
	Compositional bias	394 – 436	43	Asp-rich
Amino acid modifications
	Glycosylation	387	1	O-linked (Xyl...) (glycosaminoglycan) Potential
	Glycosylation	392	1	O-linked (Xyl...) (glycosaminoglycan) Potential
	Disulfide bond	90 ↔ 101		By similarity
	Disulfide bond	95 ↔ 111		By similarity
	Disulfide bond	139 ↔ 169		By similarity
	Disulfide bond	142 ↔ 162		By similarity
	Disulfide bond	151 ↔ 183		By similarity
	Disulfide bond	317 ↔ 341		By similarity
	Disulfide bond	352 ↔ 359		By similarity
	Disulfide bond	361 ↔ 380		By similarity
Natural variations
	Alternative sequence	64 – 66	3	Missing in isoform 1.		VSP_013598
	Alternative sequence	314 – 316	3	DPP → GKR in isoform 2.		VSP_005334
	Alternative sequence	317 – 436	120	Missing in isoform 2.		VSP_005335
	Natural variant	112	1	I → V. Corresponds to variant rs9685645 [ dbSNP | Ensembl ].		VAR_051562
Experimental info
	Sequence conflict	348	1	K → M in CAA04775. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: AAEDD1998C656FC0	FASTA	436	49,429
	10 20 30 40 50 60 MLKVSAVLCV CAAAWCSQSL AAAAAVAAAG GRSDGGNFLD DKQWLTTISQ YDKEVGQWNK 70 80 90 100 110 120 FRDEVEDDYF RTWSPGKPFD QALDPAKDPC LKMKCSRHKV CIAQDSQTAV CISHRRLTHR 130 140 150 160 170 180 MKEAGVDHRQ WRGPILSTCK QCPVVYPSPV CGSDGHTYSF QCKLEYQACV LGKQISVKCE 190 200 210 220 230 240 GHCPCPSDKP TSTSRNVKRA CSDLEFREVA NRLRDWFKAL HESGSQNKKT KTLLRPERSR 250 260 270 280 290 300 FDTSILPICK DSLGWMFNRL DTNYDLLLDQ SELRSIYLDK NEQCTKAFFN SCDTYKDSLI 310 320 330 340 350 360 SNNEWCYCFQ RQQDPPCQTE LSNIQKRQGV KKLLGQYIPL CDEDGYYKPT QCHGSVGQCW 370 380 390 400 410 420 CVDRYGNEVM GSRINGVADC AIDFEISGDF ASGDFHEWTD DEDDEDDIMN DEDEIEDDDE 430 DEGDDDDGGD DHDVYI
	Isoform 1 [UniParc]. Checksum: B697CDE58F7028AF	FASTA	433	49,072
	10 20 30 40 50 60 MLKVSAVLCV CAAAWCSQSL AAAAAVAAAG GRSDGGNFLD DKQWLTTISQ YDKEVGQWNK 70 80 90 100 110 120 FRDDDYFRTW SPGKPFDQAL DPAKDPCLKM KCSRHKVCIA QDSQTAVCIS HRRLTHRMKE 130 140 150 160 170 180 AGVDHRQWRG PILSTCKQCP VVYPSPVCGS DGHTYSFQCK LEYQACVLGK QISVKCEGHC 190 200 210 220 230 240 PCPSDKPTST SRNVKRACSD LEFREVANRL RDWFKALHES GSQNKKTKTL LRPERSRFDT 250 260 270 280 290 300 SILPICKDSL GWMFNRLDTN YDLLLDQSEL RSIYLDKNEQ CTKAFFNSCD TYKDSLISNN 310 320 330 340 350 360 EWCYCFQRQQ DPPCQTELSN IQKRQGVKKL LGQYIPLCDE DGYYKPTQCH GSVGQCWCVD 370 380 390 400 410 420 RYGNEVMGSR INGVADCAID FEISGDFASG DFHEWTDDED DEDDIMNDED EIEDDDEDEG 430 DDDDGGDDHD VYI
	Isoform 2 (N-tes) [UniParc]. Checksum: 6607E14FC72D4F44	FASTA	316	35,950
	10 20 30 40 50 60 MLKVSAVLCV CAAAWCSQSL AAAAAVAAAG GRSDGGNFLD DKQWLTTISQ YDKEVGQWNK 70 80 90 100 110 120 FRDEVEDDYF RTWSPGKPFD QALDPAKDPC LKMKCSRHKV CIAQDSQTAV CISHRRLTHR 130 140 150 160 170 180 MKEAGVDHRQ WRGPILSTCK QCPVVYPSPV CGSDGHTYSF QCKLEYQACV LGKQISVKCE 190 200 210 220 230 240 GHCPCPSDKP TSTSRNVKRA CSDLEFREVA NRLRDWFKAL HESGSQNKKT KTLLRPERSR 250 260 270 280 290 300 FDTSILPICK DSLGWMFNRL DTNYDLLLDQ SELRSIYLDK NEQCTKAFFN SCDTYKDSLI 310 SNNEWCYCFQ RQQGKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of testican-3, a novel member of brain-specific, calcium-binding proteoglycans." Vannahme C., Hartmann U., Goesling S., Kohfeldt E., Timpl R., Paulsson M., Maurer P. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Brain.
[2]	"Suppression of membrane-type 1 matrix metalloproteinase (MMP)-mediated MMP-2 activation and tumor invasion by testican 3 and its splicing variant gene product, N-Tes." Nakada M., Yamada A., Takino T., Miyamori H., Takahashi T., Yamashita J., Sato H. Cancer Res. 61:8896-8902(2001) [PubMed: 11751414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION. Tissue: Fetal kidney.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain and Corpus callosum.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ001454 mRNA. Translation: CAA04775.1. AB056866 mRNA. Translation: BAB64908.1. AY358973 mRNA. Translation: AAQ89332.1. AK091078 mRNA. Translation: BAG52279.1. AK313042 mRNA. Translation: BAG35874.1. CH471056 Genomic DNA. Translation: EAX04811.1. BC000460 mRNA. Translation: AAH00460.1. BC003017 mRNA. Translation: AAH03017.1. BC013983 mRNA. Translation: AAH13983.1.
IPI	IPI00419590. IPI00478890. IPI00555693.
RefSeq	NP_001035249.1. NM_001040159.1. NP_001191283.1. NM_001204354.1. NP_001191287.1. NM_001204358.1. NP_058646.2. NM_016950.2.
UniGene	Hs.481133.
3D structure databases
ProteinModelPortal	Q9BQ16.
SMR	Q9BQ16. Positions 85-313, 315-382.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4830490.
STRING	Q9BQ16.
Protein family/group databases
MEROPS	I01.980.
PTM databases
PhosphoSite	Q9BQ16.
Polymorphism databases
DMDM	67473703.
Proteomic databases
PRIDE	Q9BQ16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000357154; ENSP00000349677; ENSG00000196104.
GeneID	50859.
KEGG	hsa:50859.
UCSC	uc003iri.1. human. uc003irj.1. human. uc003irk.2. human.
Organism-specific databases
CTD	50859.
GeneCards	GC04M167654.
H-InvDB	HIX0004621.
HGNC	HGNC:13565. SPOCK3.
MIM	607989. gene.
neXtProt	NX_Q9BQ16.
PharmGKB	PA134977377.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12207.
GeneTree	ENSGT00510000046429.
HOGENOM	HBG444198.
HOVERGEN	HBG026595.
InParanoid	Q9BQ16.
OMA	QCKLEYQ.
OrthoDB	EOG44MXSB.
PhylomeDB	Q9BQ16.
Gene expression databases
ArrayExpress	Q9BQ16.
Bgee	Q9BQ16.
CleanEx	HS_SPOCK3.
Genevestigator	Q9BQ16.
GermOnline	ENSG00000196104. Homo sapiens.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR002350. Prot_inh_Kazal. IPR011497. Prot_Inh_Kazal_2. IPR019577. SPARC/Testican_Ca-bd-dom. IPR000716. Thyroglobulin_1. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:4.10.800.10. Thyroglobulin_1. 1 hit.
KO	K08136.
Pfam	PF07648. Kazal_2. 1 hit. PF10591. SPARC_Ca_bdg. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
SMART	SM00280. KAZAL. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS51465. KAZAL_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	53356.
SOURCE	Search...

Entry information

Entry name

TICN3_HUMAN

Accession

Primary (citable) accession number: Q9BQ16
Secondary accession number(s): B2R7M7 , B3KR67, O75705, Q6UW53, Q96Q26

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	May 10, 2005
Last modified:	January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.