Q9BPZ3 (PAIP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyadenylate-binding protein-interacting protein 2
Short name=PABP-interacting protein 2
Short name=PAIP-2
Short name=Poly(A)-binding protein-interacting protein 2

Gene names

Name:	PAIP2
Synonyms:	PAIP2A
ORF Names:	HSPC218

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a repressor in the regulation of translation initiation of poly(A)-containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. Displaces the interaction of PABPC1 with poly(A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly(A) RNP structure organization. Ref.1
Subunit structure	Interacts with the second and third RRM domains and C-terminus regions of PABPC1 in a 2:1 stoichiometry. Ref.1 Ref.7
Subcellular location	Cytoplasm Ref.1.
Domain	Only the PABPC1-interacting motif-1 (PAM1) interferes with the binding of PABPC1 to poly(A) RNA and translation initiation.
Sequence similarities	Belongs to the PAIP2 family.

Ontologies

Keywords
Biological process	Translation regulation
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of translational initiation Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB translation repressor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 127

127

Polyadenylate-binding protein-interacting protein 2

PRO_0000058179

Regions

Region

22 – 75

54

PABPC1-interacting motif-1 (PAM1)

Region

105 – 120

16

PABPC1-interacting motif-2 (PAM2)

Compositional bias

33 – 72

40

Glu-rich

Experimental info

Sequence conflict

98

1

D → E in CAB66575. Ref.3

Sequence conflict

116

1

K → T in AAF36138. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

127

Helix Strand Turn

Details...

Turn

113 – 114

2

Turn

120 – 121

2

Turn

124 – 125

2

Sequences

	Sequence		Length	Mass (Da)
	Q9BPZ3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CAC27C537E5C6E22	FASTA	127	14,984
	10 20 30 40 50 60 MKDPSRSSTS PSIINEDVII NGHSHEDDNP FAEYMWMENE EEFNRQIEEE LWEEEFIERC 70 80 90 100 110 120 FQEMLEEEEE HEWFIPARDL PQTMDQIQDQ FNDLVISDGS SLEDLVVKSN LNPNAKEFVP GVKYGNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Translational repression by a novel partner of human poly(A) binding protein, Paip2." Khaleghpour K., Svitkin Y.V., Craig A.W.B., DeMaria C.T., Deo R.C., Burley S.K., Sonenberg N. Mol. Cell 7:205-216(2001) [PubMed: 11172725] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION IN TRANSLATION INHIBITION, INTERACTION WITH PABPC1. Tissue: Placenta.
[2]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[3]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye and Testis.
[7]	"Dual interactions of the translational repressor Paip2 with poly(A) binding protein." Khaleghpour K., Kahvejian A., De Crescenzo G., Roy G., Svitkin Y.V., Imataka H., O'Connor-McCourt M., Sonenberg N. Mol. Cell. Biol. 21:5200-5213(2001) [PubMed: 11438674] [Abstract] Cited for: INTERACTION WITH PABPC1.
[8]	"Structure and function of the C-terminal PABC domain of human poly(A)-binding protein." Kozlov G., Trempe J.F., Khaleghpour K., Kahvejian A., Ekiel I., Gehring K. Proc. Natl. Acad. Sci. U.S.A. 98:4409-4413(2001) [PubMed: 11287632] [Abstract] Cited for: STRUCTURE BY NMR OF 106-127.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF317675 mRNA. Translation: AAK11562.1.
AF151052 mRNA. Translation: AAF36138.1.
AL136640 mRNA. Translation: CAB66575.1.
AK314672 mRNA. Translation: BAG37228.1.
CH471062 Genomic DNA. Translation: EAW62107.1.
CH471062 Genomic DNA. Translation: EAW62108.1.
CH471062 Genomic DNA. Translation: EAW62111.1.
CH471062 Genomic DNA. Translation: EAW62112.1.
BC001716 mRNA. Translation: AAH01716.1.
BC048106 mRNA. Translation: AAH48106.1.
BC062718 mRNA. Translation: AAH62718.1.

IPI

IPI00304483.

RefSeq

NP_001028284.1. NM_001033112.1.
NP_057564.3. NM_016480.3.

UniGene

Hs.396644.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JGN	NMR	-	B	106-127	[»]
3KUS	X-ray	1.40	C/D	109-123	[»]
3KUT	X-ray	1.50	C/D	109-125	[»]

ProteinModelPortal

Q9BPZ3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9BPZ3. 2 interactions.

MINT

MINT-1899106.

STRING

Q9BPZ3.

Polymorphism databases

DMDM

46397013.

Proteomic databases

PeptideAtlas

Q9BPZ3.

PRIDE

Q9BPZ3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000265192; ENSP00000265192; ENSG00000120727.
ENST00000394795; ENSP00000378275; ENSG00000120727.

GeneID

51247.

KEGG

hsa:51247.

UCSC

uc003led.1. human.

Organism-specific databases

CTD

51247.

GeneCards

GC05P138706.

H-InvDB

HIX0005220.

HGNC

HGNC:17970. PAIP2.

HPA

HPA035945.

MIM

605604. gene.

neXtProt

NX_Q9BPZ3.

PharmGKB

PA134897284.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21079.

GeneTree

ENSGT00390000017284.

HOGENOM

HBG717342.

HOVERGEN

HBG053493.

InParanoid

Q9BPZ3.

OMA

PGVKYLN.

PhylomeDB

Q9BPZ3.

Gene expression databases

ArrayExpress

Q9BPZ3.

Bgee

Q9BPZ3.

CleanEx

HS_PAIP2.

Genevestigator

Q9BPZ3.

GermOnline

ENSG00000120727. Homo sapiens.

Family and domain databases

InterPro

IPR009818. Ataxin-2_C.
[Graphical view]

Pfam

PF07145. PAM2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

54373.

SOURCE

Search...

Entry information

Entry name

PAIP2_HUMAN

Accession

Primary (citable) accession number: Q9BPZ3
Secondary accession number(s): B2RBI1 , D3DQC6, Q49A06, Q9H0Y5, Q9P0Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.