Q9BPV8 (P2Y13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2Y purinoceptor 13
Short name=P2Y13

Alternative name(s):
G-protein coupled receptor 86
G-protein coupled receptor 94

Gene names

Name:	P2RY13
Synonyms:	GPR86, GPR94
ORF Names:	FKSG77

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Strong expression in spleen and adult brain. Lower expression in placenta, lung, liver, spinal cord, thymus, small intestine, uterus, stomach, testis, fetal brain, and adrenal gland. Not detected in pancreas, heart, kidney, skeletal muscle, ovary or fetal aorta. Clearly detected in lymph node and bone marrow, weakly detected in peripheral blood mononuclear cells (PBMC) and in peripheral blood leukocytes (PBL), but not detected in polymorphonuclear cells (PMN). In the brain, detected in all brain regions examined.
Miscellaneous	Stimulation by ADP in stably transfected CHO cells resulted in inhibition of adenylyl cyclase and the phosphorylation of the MAP kinases MAPK3 and MAPK1 in a pertussis toxin-sensitive way. Inhibition of adenylyl cyclase and phosphorylation of the MAP kinases are transduction mechanisms that involve G(i) proteins.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence AAH29363.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH41116.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG36024.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Non-traceable author statement. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BPV8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BPV8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	P2Y purinoceptor 13		PRO_0000070040
Regions
	Topological domain	1 – 49	49	Extracellular Potential
	Transmembrane	50 – 70	21	Helical; Name=1; Potential
	Topological domain	71 – 77	7	Cytoplasmic Potential
	Transmembrane	78 – 98	21	Helical; Name=2; Potential
	Topological domain	99 – 117	19	Extracellular Potential
	Transmembrane	118 – 138	21	Helical; Name=3; Potential
	Topological domain	139 – 161	23	Cytoplasmic Potential
	Transmembrane	162 – 182	21	Helical; Name=4; Potential
	Topological domain	183 – 211	29	Extracellular Potential
	Transmembrane	212 – 232	21	Helical; Name=5; Potential
	Topological domain	233 – 252	20	Cytoplasmic Potential
	Transmembrane	253 – 273	21	Helical; Name=6; Potential
	Topological domain	274 – 300	27	Extracellular Potential
	Transmembrane	301 – 321	21	Helical; Name=7; Potential
	Topological domain	322 – 333	12	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	31	1	N-linked (GlcNAc...) Potential
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Disulfide bond	116 ↔ 194		By similarity
Natural variations
	Alternative sequence	1 – 21	21	Missing in isoform 2.		VSP_036226
	Natural variant	179	1	T → M. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.8 Ref.9 Ref.10 Corresponds to variant rs1466684 [ dbSNP | Ensembl ].		VAR_028299

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 8, 2011. Version 3. Checksum: A2CE5F8ED84C4CAF	FASTA	354	40,789
	10 20 30 40 50 60 MTAAIRRQRE LSILPKVTLE AMNTTVMQGF NRSERCPRDT RIVQLVFPAL YTVVFLTGIL 70 80 90 100 110 120 LNTLALWVFV HIPSSSTFII YLKNTLVADL IMTLMLPFKI LSDSHLAPWQ LRAFVCRFSS 130 140 150 160 170 180 VIFYETMYVG IVLLGLIAFD RFLKIIRPLR NIFLKKPVFA KTVSIFIWFF LFFISLPNTI 190 200 210 220 230 240 LSNKEATPSS VKKCASLKGP LGLKWHQMVN NICQFIFWTV FILMLVFYVV IAKKVYDSYR 250 260 270 280 290 300 KSKSKDRKNN KKLEGKVFVV VAVFFVCFAP FHFARVPYTH SQTNNKTDCR LQNQLFIAKE 310 320 330 340 350 TTLFLAATNI CMDPLIYIFL CKKFTEKLPC MQGRKTTASS QENHSSQTDN ITLG
	Isoform 2 [UniParc]. Checksum: 3F88E7EAC8F5428F	FASTA	333	38,410
	10 20 30 40 50 60 MNTTVMQGFN RSERCPRDTR IVQLVFPALY TVVFLTGILL NTLALWVFVH IPSSSTFIIY 70 80 90 100 110 120 LKNTLVADLI MTLMLPFKIL SDSHLAPWQL RAFVCRFSSV IFYETMYVGI VLLGLIAFDR 130 140 150 160 170 180 FLKIIRPLRN IFLKKPVFAK TVSIFIWFFL FFISLPNTIL SNKEATPSSV KKCASLKGPL 190 200 210 220 230 240 GLKWHQMVNN ICQFIFWTVF ILMLVFYVVI AKKVYDSYRK SKSKDRKNNK KLEGKVFVVV 250 260 270 280 290 300 AVFFVCFAPF HFARVPYTHS QTNNKTDCRL QNQLFIAKET TLFLAATNIC MDPLIYIFLC 310 320 330 KKFTEKLPCM QGRKTTASSQ ENHSSQTDNI TLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Discovery and mapping of ten novel G protein-coupled receptor genes." Lee D.K., Nguyen T., Lynch K.R., Cheng R., Vanti W.B., Arkhitko O., Lewis T., Evans J.F., George S.R., O'Dowd B.F. Gene 275:83-91(2001) [PubMed: 11574155] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 2), VARIANT MET-179.
[2]	"Identification of a novel human ADP receptor coupled to Gi." Communi D., Gonzalez N.S., Detheux M., Brezillon S., Lannoy V., Parmentier M., Boeynaems J.-M. J. Biol. Chem. 276:41479-41485(2001) [PubMed: 11546776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, VARIANT MET-179. Tissue: Spleen.
[3]	"An expressed sequence tag (EST) data mining strategy succeeding in the discovery of new G-protein coupled receptors." Wittenberger T., Schaller H.C., Hellebrand S. J. Mol. Biol. 307:799-813(2001) [PubMed: 11273702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT MET-179. Tissue: Amygdala, Caudate nucleus, Fetal brain and Placenta.
[4]	"Molecular cloning of FKSG77, a novel gene encoding a putative G-protein-coupled receptor." Wang Y.-G., Gong L. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT MET-179. Tissue: Heart.
[5]	"Identification of G protein-coupled receptor genes from the human genome sequence." Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S. FEBS Lett. 520:97-101(2002) [PubMed: 12044878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT MET-179.
[7]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed: 16641997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT MET-179.
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT MET-179. Tissue: Brain and Placenta.
[10]	"Molecular cloning of a probable G protein-coupled receptor with three transmembrane domains." Zhang W., Wan T., Cao X. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 172-354, VARIANT MET-179.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF411113 Genomic DNA. Translation: AAL26484.1.
AF406692 mRNA. Translation: AAL01038.1.
AF295368 mRNA. Translation: AAK01864.1.
AF345565 mRNA. Translation: AAK29068.1.
AB083597 Genomic DNA. Translation: BAB89310.1.
AK313208 mRNA. Translation: BAG36024.1. Different initiation.
AC024886 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78805.1.
BC029363 mRNA. Translation: AAH29363.1. Sequence problems.
BC041116 mRNA. Translation: AAH41116.2. Different initiation.
BC066887 mRNA. Translation: AAH66887.1.
AF178982 mRNA. Translation: AAK18752.1.

IPI

IPI00216851.
IPI00789314.

RefSeq

NP_795713.2. NM_176894.2.

UniGene

Hs.546396.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B6U	model	-	A	22-354	[»]

ProteinModelPortal

Q9BPV8.

SMR

Q9BPV8. Positions 128-159, 301-334.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9BPV8.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

Q9BPV8.

Polymorphism databases

DMDM

221222470.

Proteomic databases

PRIDE

Q9BPV8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000325602; ENSP00000320376; ENSG00000181631.

GeneID

53829.

KEGG

hsa:53829.

Organism-specific databases

CTD

53829.

GeneCards

GC03M151044.

H-InvDB

HIX0200553.

HGNC

HGNC:4537. P2RY13.

MIM

606380. gene.

neXtProt

NX_Q9BPV8.

GenAtlas

Search...

Phylogenomic databases

eggNOG

maNOG11114.

GeneTree

ENSGT00550000074295.

HOVERGEN

HBG108228.

InParanoid

Q9BPV8.

OMA

AFVCRFS.

OrthoDB

EOG49S679.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

Q9BPV8.

Bgee

Q9BPV8.

CleanEx

HS_P2RY13.

Genevestigator

Q9BPV8.

GermOnline

ENSG00000181631. Homo sapiens.

Family and domain databases

InterPro

IPR000276. 7TM_GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_supfam.
IPR002286. P2_purnocptor.
IPR008109. P2Y13_purnocptor.
[Graphical view]

KO

K08388.

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00237. GPCRRHODOPSN.
PR01735. P2Y13PRNCPTR.
PR01157. P2YPURNOCPTR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

56152.

SOURCE

Search...

Entry information

Entry name

P2Y13_HUMAN

Accession

Primary (citable) accession number: Q9BPV8
Secondary accession number(s): B2R827 , Q05C50, Q6DKN4, Q8IUT5, Q8TDU7, Q9BY61

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2003
Last sequence update:	February 8, 2011
Last modified:	January 25, 2012
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.