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Protein

Enolase 2

Gene

ENO2

Organism
Toxoplasma gondii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

While ENO1 and ENO2 display similar K(m) values, the pure tachyzoite-specific enzyme (ENO2) has a threefold specific activity at V(max) compared with that of the bradyzoite-specific enolase (ENO1).

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase 1 (ENO1), Enolase 2 (ENO2)
  5. Pyruvate kinase (pk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei165SubstrateBy similarity1
Binding sitei174SubstrateBy similarity1
Active sitei217Proton donorBy similarity1
Metal bindingi252MagnesiumBy similarity1
Metal bindingi303MagnesiumBy similarity1
Binding sitei303SubstrateBy similarity1
Metal bindingi330MagnesiumBy similarity1
Binding sitei330SubstrateBy similarity1
Active sitei355Proton acceptorBy similarity1
Binding sitei406SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

SABIO-RKiQ9BPL7.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 2 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 2
2-phosphoglycerate dehydratase 2
Gene namesi
Name:ENO2
OrganismiToxoplasma gondii
Taxonomic identifieri5811 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340941 – 444Enolase 2Add BLAST444

Proteomic databases

PRIDEiQ9BPL7.

Expressioni

Developmental stagei

Expressed preferentially in the tachyzoite stage.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9BPL7.
SMRiQ9BPL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni382 – 385Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BPL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAIKDITAR QILDSRGNPT VEVDLLTDGG CFRAAVPSGA STGIYEALEL
60 70 80 90 100
RDKDQTKFMG KGVMKAVENI HKIIKPALIG KDPCDQKGID KLMVEELDGT
110 120 130 140 150
KNEWGWCKSK LGANAILAVS MACCRAGAAA KGMPLYKYIA TLAGNPTDKM
160 170 180 190 200
VMPVPFFNVI NGGSHAGNKV AMQEFMIAPV GASTIQEAIQ IGAEVYQHLK
210 220 230 240 250
VVIKKKYGLD ATNVGDEGGF APNISGATEA LDLLMEAIKV SGHEGKVKIA
260 270 280 290 300
ADVAASEFFL QDDKVYDLDF KTPNNDKSQR KTGEELRNLY KDLCQKYPFV
310 320 330 340 350
SIEDPFDQDD FHSYAQLTNE VGEKVQIVGD DLLVTNPTRI EKAVQEKACN
360 370 380 390 400
GLLLKVNQIG TVSESIEACQ LAQKNKWGVM VSHRSGETED SFIADLVVGL
410 420 430 440
RTGQIKTGAP CRSERLCKYN QLMRIEESLG SDCQYAGAGF RHPN
Length:444
Mass (Da):48,290
Last modified:June 1, 2001 - v1
Checksum:i4029B1EA9024D1A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123457 mRNA. Translation: AAG60329.1.
AY155668 Genomic DNA. Translation: AAP24057.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO2_TOXGO
AccessioniPrimary (citable) accession number: Q9BPL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: July 5, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families