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Q9BN21 (CLE1_HETGL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CLAVATA3/ESR (CLE)-related protein 1

Short name=CLE-like peptide 1
Short name=Hg-SYV46
Alternative name(s):
Esophageal gland cell secretory protein 1
Gene names
Name:CLE1
Synonyms:2B10, HSP1, SYV46
OrganismHeterodera glycines (Soybean cyst nematode worm)
Taxonomic identifier51029 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaTylenchidaTylenchinaTylenchoideaHeteroderidaeHeteroderinaeHeterodera

Protein attributes

Sequence length139 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mimics host plant CLE extracellular signal peptides that regulate cell fate. May play a role in the differentiation or division of feeding cells (syncytia) induced in plant roots during infection By similarity. Ref.2 Ref.4

Subcellular location

Secreted. Host cytoplasm. Host extracellular space. Secretedextracellular spaceapoplast. Note: Present in secretory granules within the dorsal esophageal gland secretory cell and in the dorsal gland ampulla (collecting reservoir) at the base of the nematode stylet. Secreted into host root cells via the nematode stylet to transform the recipient cells into enlarged multinucleate feeding cells called giant-cells or syncytia. Secreted to the host apoplasm from its cytoplasm via a plant secretory pathway. Ref.2 Ref.4

Tissue specificity

Highly expressed exclusively within the dorsal esophageal gland cell during syncytium formation in host plants (at protein level). Ref.1 Ref.2 Ref.3 Ref.4

Developmental stage

Strongly up-regulated during root colonization, from the onset of syncytium formation by parasitic second-stage juveniles (pJ2) through the J3?J4 molts of sedentary life stages that become adult females. Ref.2

Miscellaneous

Failed to trigger CLE-like cell fate regulation in Arabidopsis thaliana, a non-host plant.

Sequence similarities

Belongs to the CLV3/ESR signal peptide family.

Ontologies

Keywords
   Biological processDifferentiation
   Cellular componentApoplast
Host cytoplasm
Secreted
   DomainCoiled coil
Signal
   PTMGlycoprotein
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular space of host

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 139117CLAVATA3/ESR (CLE)-related protein 1
PRO_0000401215

Regions

Region23 – 9068Required for secretion from the host cytoplasm to the host apoplasm
Coiled coil100 – 12526 Potential
Motif128 – 13912CLE
Compositional bias92 – 965Poly-Pro

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation871N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9BN21 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: B29F9676CA3A73BC

FASTA13914,504
        10         20         30         40         50         60 
MPNIFKILLI VLLAVVSFRL SASTGDKKTA NDGSGNNSSA GIGTKIKRIV TAGLLFTSLA 

        70         80         90        100        110        120 
TGGAEAIGRS NAQGGNAAGL VPSHLTNRSM APPPPPAQFE KGAATRVEKM RAQLRELAEK 

       130 
MTDKDPKRLS PSGPDPHHH 

« Hide

References

[1]"Signal peptide-selection of cDNA cloned directly from the esophageal gland cells of the soybean cyst nematode Heterodera glycines."
Wang X., Allen R., Ding X., Goellner M., Maier T., de Boer J.M., Baum T.J., Hussey R.S., Davis E.L.
Mol. Plant Microbe Interact. 14:536-544(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Esophagus and Gland.
[2]"Dual roles for the variable domain in protein trafficking and host-specific recognition of Heterodera glycines CLE effector proteins."
Wang J., Lee C., Replogle A., Joshi S., Korkin D., Hussey R., Baum T.J., Davis E.L., Wang X., Mitchum M.G.
New Phytol. 187:1003-1017(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
[3]"The parasitome of the phytonematode Heterodera glycines."
Gao B., Allen R., Maier T., Davis E.L., Baum T.J., Hussey R.S.
Mol. Plant Microbe Interact. 16:720-726(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
Tissue: Gland.
[4]"A parasitism gene from a plant-parasitic nematode with function similar to CLAVATA3/ESR (CLE) of Arabidopsis thaliana."
Wang X., Mitchum M.G., Gao B., Li C., Diab H., Baum T.J., Hussey R.S., Davis E.L.
Mol. Plant Pathol. 6:187-191(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]Erratum
Davis E.L.
Mol. Plant Pathol. 10:151-151(2009)
[6]"Diverse and conserved roles of CLE peptides."
Mitchum M.G., Wang X., Davis E.L.
Curr. Opin. Plant Biol. 11:75-81(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Web resources

Protein Spotlight

the root of the problem - Issue 151 of July 2013

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF273728 mRNA. Translation: AAG21331.2.
FJ503004 Genomic DNA. Translation: ACT32609.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameCLE1_HETGL
AccessionPrimary (citable) accession number: Q9BN21
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: June 1, 2001
Last modified: October 16, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries