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Q9BN01

- AMYB_DROYA

UniProt

Q9BN01 - AMYB_DROYA

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Protein

Alpha-amylase B

Gene

Amy-d

Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca(2+) ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl(-) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161CalciumBy similarity
Metal bindingi165 – 1651Calcium; via carbonyl oxygenBy similarity
Metal bindingi174 – 1741CalciumBy similarity
Binding sitei202 – 2021ChlorideBy similarity
Active sitei204 – 2041By similarity
Metal bindingi208 – 2081Calcium; via carbonyl oxygenBy similarity
Active sitei241 – 2411By similarity
Binding sitei304 – 3041ChlorideBy similarity
Sitei306 – 3061Transition state stabilizerBy similarity
Binding sitei343 – 3431ChlorideBy similarity

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase B (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy-d
ORF Names:GE11648
OrganismiDrosophila yakuba (Fruit fly)
Taxonomic identifieri7245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000002282: Chromosome 2R, UP000002282: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0013165. Dyak\Amy-d.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 494476Alpha-amylase BPRO_0000001367Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acidBy similarity
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi153 ↔ 167By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7245.FBpp0256658.

Structurei

3D structure databases

ProteinModelPortaliQ9BN01.
SMRiQ9BN01. Positions 21-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0366.
KOiK01176.
OrthoDBiEOG7RJPR2.
PhylomeDBiQ9BN01.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BN01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL
60 70 80 90 100
GPNGFAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEQQFASMVK
110 120 130 140 150
RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSTKSFP GVPYSSLDFN
160 170 180 190 200
PTCSINNYND ANQVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG
210 220 230 240 250
FRVDAAKHMW PADLAVIYGR LKTLNTDHGF NSGSKAYIVQ EVIDMGGEAI
260 270 280 290 300
SKSEYTGLGA VTEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV
310 320 330 340 350
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF
360 370 380 390 400
SDTDQGPPTT DGHNIASPVF NSDNSCSGGW VCEHRWRQIY NMVAFRNAVG
410 420 430 440 450
SDAIQNWWDN GSNQIAFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV
460 470 480 490
ISGSKSGSSC TGKTVSVGSD GRASIYLGSS EDDGVLAIHV NAKL
Length:494
Mass (Da):53,688
Last modified:March 29, 2004 - v2
Checksum:iEBDC615F3C0044B8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti179 – 1802NS → DP in strain: LO4.
Natural varianti195 – 1951D → N in strain: LO4.
Natural varianti330 – 3301A → V in strain: LO4.
Natural varianti381 – 3811V → A in strain: LO4.
Natural varianti405 – 4051Q → P in strain: LO4.
Natural varianti427 – 4271F → Y in strain: LO4.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17737 Genomic DNA. Translation: BAA04590.1.
AF280886 Genomic DNA. Translation: AAG60011.1.
AF280887 Genomic DNA. Translation: AAG60012.1.
AF280888 Genomic DNA. Translation: AAG60013.1.
AF280889 Genomic DNA. Translation: AAG60014.1.
CM000158 Genomic DNA. Translation: EDW92184.1.
PIRiS58946.
RefSeqiXP_002092472.1. XM_002092436.1.

Genome annotation databases

EnsemblMetazoaiFBtr0258166; FBpp0256658; FBgn0013165.
GeneIDi6531681.
KEGGidya:Dyak_GE11648.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17737 Genomic DNA. Translation: BAA04590.1 .
AF280886 Genomic DNA. Translation: AAG60011.1 .
AF280887 Genomic DNA. Translation: AAG60012.1 .
AF280888 Genomic DNA. Translation: AAG60013.1 .
AF280889 Genomic DNA. Translation: AAG60014.1 .
CM000158 Genomic DNA. Translation: EDW92184.1 .
PIRi S58946.
RefSeqi XP_002092472.1. XM_002092436.1.

3D structure databases

ProteinModelPortali Q9BN01.
SMRi Q9BN01. Positions 21-494.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7245.FBpp0256658.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0258166 ; FBpp0256658 ; FBgn0013165 .
GeneIDi 6531681.
KEGGi dya:Dyak_GE11648.

Organism-specific databases

FlyBasei FBgn0013165. Dyak\Amy-d.

Phylogenomic databases

eggNOGi COG0366.
KOi K01176.
OrthoDBi EOG7RJPR2.
PhylomeDBi Q9BN01.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view ]
PRINTSi PR00110. ALPHAAMYLASE.
SMARTi SM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular evolution of the duplicated Amy locus in the Drosophila melanogaster species subgroup: concerted evolution only in the coding region and an excess of nonsynonymous substitutions in speciation."
    Shibata H., Yamazaki T.
    Genetics 141:223-236(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Divergence between Drosophila santomea and allopatric or sympatric populations of D. yakuba using paralogous amylase genes and migration scenarios along the Cameroon volcanic line."
    Cariou M.-L., Silvain J.-F., Daubin V., Da Lage J.-L., Lachaise D.
    Mol. Ecol. 10:649-660(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LBV1, LO4, SA2 and SA3.
  3. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tai18E2 / Tucson 14021-0261.01.

Entry informationi

Entry nameiAMYB_DROYA
AccessioniPrimary (citable) accession number: Q9BN01
Secondary accession number(s): B4P8G7, P51548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 29, 2004
Last modified: November 26, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3