SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9BN01

- AMYB_DROYA

UniProt

Q9BN01 - AMYB_DROYA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Alpha-amylase B
Gene
Amy-d, GE11648
Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Binds 1 calcium ion per subunit By similarity.
Binds 1 chloride ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161Calcium By similarity
Metal bindingi165 – 1651Calcium; via carbonyl oxygen By similarity
Metal bindingi174 – 1741Calcium By similarity
Binding sitei202 – 2021Chloride By similarity
Active sitei204 – 2041 By similarity
Metal bindingi208 – 2081Calcium; via carbonyl oxygen By similarity
Active sitei241 – 2411 By similarity
Binding sitei304 – 3041Chloride By similarity
Sitei306 – 3061Transition state stabilizer By similarity
Binding sitei343 – 3431Chloride By similarity

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase B (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy-d
ORF Names:GE11648
OrganismiDrosophila yakuba (Fruit fly)
Taxonomic identifieri7245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000002282: Chromosome 2R, UP000002282: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0013165. Dyak\Amy-d.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 By similarity
Add
BLAST
Chaini19 – 494476Alpha-amylase B
PRO_0000001367Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acid By similarity
Disulfide bondi46 ↔ 102 By similarity
Disulfide bondi153 ↔ 167 By similarity
Disulfide bondi376 ↔ 382 By similarity
Disulfide bondi448 ↔ 460 By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi7245.FBpp0256658.

Structurei

3D structure databases

ProteinModelPortaliQ9BN01.
SMRiQ9BN01. Positions 21-494.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0366.
KOiK01176.
OrthoDBiEOG7RJPR2.
PhylomeDBiQ9BN01.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BN01-1 [UniParc]FASTAAdd to Basket

« Hide

MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL    50
GPNGFAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEQQFASMVK 100
RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSTKSFP GVPYSSLDFN 150
PTCSINNYND ANQVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG 200
FRVDAAKHMW PADLAVIYGR LKTLNTDHGF NSGSKAYIVQ EVIDMGGEAI 250
SKSEYTGLGA VTEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV 300
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF 350
SDTDQGPPTT DGHNIASPVF NSDNSCSGGW VCEHRWRQIY NMVAFRNAVG 400
SDAIQNWWDN GSNQIAFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV 450
ISGSKSGSSC TGKTVSVGSD GRASIYLGSS EDDGVLAIHV NAKL 494
Length:494
Mass (Da):53,688
Last modified:March 29, 2004 - v2
Checksum:iEBDC615F3C0044B8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti179 – 1802NS → DP in strain: LO4.
Natural varianti195 – 1951D → N in strain: LO4.
Natural varianti330 – 3301A → V in strain: LO4.
Natural varianti381 – 3811V → A in strain: LO4.
Natural varianti405 – 4051Q → P in strain: LO4.
Natural varianti427 – 4271F → Y in strain: LO4.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17737 Genomic DNA. Translation: BAA04590.1.
AF280886 Genomic DNA. Translation: AAG60011.1.
AF280887 Genomic DNA. Translation: AAG60012.1.
AF280888 Genomic DNA. Translation: AAG60013.1.
AF280889 Genomic DNA. Translation: AAG60014.1.
CM000158 Genomic DNA. Translation: EDW92184.1.
PIRiS58946.
RefSeqiXP_002092472.1. XM_002092436.1.

Genome annotation databases

EnsemblMetazoaiFBtr0258166; FBpp0256658; FBgn0013165.
GeneIDi6531681.
KEGGidya:Dyak_GE11648.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17737 Genomic DNA. Translation: BAA04590.1 .
AF280886 Genomic DNA. Translation: AAG60011.1 .
AF280887 Genomic DNA. Translation: AAG60012.1 .
AF280888 Genomic DNA. Translation: AAG60013.1 .
AF280889 Genomic DNA. Translation: AAG60014.1 .
CM000158 Genomic DNA. Translation: EDW92184.1 .
PIRi S58946.
RefSeqi XP_002092472.1. XM_002092436.1.

3D structure databases

ProteinModelPortali Q9BN01.
SMRi Q9BN01. Positions 21-494.
ModBasei Search...

Protein-protein interaction databases

STRINGi 7245.FBpp0256658.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0258166 ; FBpp0256658 ; FBgn0013165 .
GeneIDi 6531681.
KEGGi dya:Dyak_GE11648.

Organism-specific databases

FlyBasei FBgn0013165. Dyak\Amy-d.

Phylogenomic databases

eggNOGi COG0366.
KOi K01176.
OrthoDBi EOG7RJPR2.
PhylomeDBi Q9BN01.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view ]
PRINTSi PR00110. ALPHAAMYLASE.
SMARTi SM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular evolution of the duplicated Amy locus in the Drosophila melanogaster species subgroup: concerted evolution only in the coding region and an excess of nonsynonymous substitutions in speciation."
    Shibata H., Yamazaki T.
    Genetics 141:223-236(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Divergence between Drosophila santomea and allopatric or sympatric populations of D. yakuba using paralogous amylase genes and migration scenarios along the Cameroon volcanic line."
    Cariou M.-L., Silvain J.-F., Daubin V., Da Lage J.-L., Lachaise D.
    Mol. Ecol. 10:649-660(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LBV1, LO4, SA2 and SA3.
  3. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tai18E2 / Tucson 14021-0261.01.

Entry informationi

Entry nameiAMYB_DROYA
AccessioniPrimary (citable) accession number: Q9BN01
Secondary accession number(s): B4P8G7, P51548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 29, 2004
Last modified: July 9, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi