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Protein

Alpha-amylase B

Gene

Amy-d

Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116CalciumBy similarity1
Metal bindingi165Calcium; via carbonyl oxygenBy similarity1
Metal bindingi174CalciumBy similarity1
Binding sitei202ChlorideBy similarity1
Active sitei204NucleophileBy similarity1
Metal bindingi208Calcium; via carbonyl oxygenBy similarity1
Active sitei241Proton donorBy similarity1
Binding sitei304ChlorideBy similarity1
Sitei306Transition state stabilizerBy similarity1
Binding sitei343ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase B (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy-d
ORF Names:GE11648
OrganismiDrosophila yakuba (Fruit fly)
Taxonomic identifieri7245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000002282 Componentsi: Chromosome 2R, Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0013165 Dyak\Amy-d

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000000136719 – 494Alpha-amylase BAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi153 ↔ 167By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Gene expression databases

BgeeiFBgn0013165

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7245.FBpp0256658

Structurei

3D structure databases

ProteinModelPortaliQ9BN01
SMRiQ9BN01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
KOiK01176
OMAiWKCQHAW
OrthoDBiEOG091G07V6
PhylomeDBiQ9BN01

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BN01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL
60 70 80 90 100
GPNGFAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEQQFASMVK
110 120 130 140 150
RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSTKSFP GVPYSSLDFN
160 170 180 190 200
PTCSINNYND ANQVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG
210 220 230 240 250
FRVDAAKHMW PADLAVIYGR LKTLNTDHGF NSGSKAYIVQ EVIDMGGEAI
260 270 280 290 300
SKSEYTGLGA VTEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV
310 320 330 340 350
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF
360 370 380 390 400
SDTDQGPPTT DGHNIASPVF NSDNSCSGGW VCEHRWRQIY NMVAFRNAVG
410 420 430 440 450
SDAIQNWWDN GSNQIAFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV
460 470 480 490
ISGSKSGSSC TGKTVSVGSD GRASIYLGSS EDDGVLAIHV NAKL
Length:494
Mass (Da):53,688
Last modified:March 29, 2004 - v2
Checksum:iEBDC615F3C0044B8
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti179 – 180NS → DP in strain: LO4. 2
Natural varianti195D → N in strain: LO4. 1
Natural varianti330A → V in strain: LO4. 1
Natural varianti381V → A in strain: LO4. 1
Natural varianti405Q → P in strain: LO4. 1
Natural varianti427F → Y in strain: LO4. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17737 Genomic DNA Translation: BAA04590.1
AF280886 Genomic DNA Translation: AAG60011.1
AF280887 Genomic DNA Translation: AAG60012.1
AF280888 Genomic DNA Translation: AAG60013.1
AF280889 Genomic DNA Translation: AAG60014.1
CM000158 Genomic DNA Translation: EDW92184.1
PIRiS58946
RefSeqiXP_002092472.1, XM_002092436.2

Genome annotation databases

EnsemblMetazoaiFBtr0258166; FBpp0256658; FBgn0013165
GeneIDi6531681
KEGGidya:Dyak_GE11648

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiAMYB_DROYA
AccessioniPrimary (citable) accession number: Q9BN01
Secondary accession number(s): B4P8G7, P51548
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 29, 2004
Last modified: February 28, 2018
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health