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Protein

Alpha-amylase B

Gene

Amy-d

Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116CalciumBy similarity1
Metal bindingi165Calcium; via carbonyl oxygenBy similarity1
Metal bindingi174CalciumBy similarity1
Binding sitei202ChlorideBy similarity1
Active sitei204NucleophileBy similarity1
Metal bindingi208Calcium; via carbonyl oxygenBy similarity1
Active sitei241Proton donorBy similarity1
Binding sitei304ChlorideBy similarity1
Sitei306Transition state stabilizerBy similarity1
Binding sitei343ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase B (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:Amy-d
ORF Names:GE11648
OrganismiDrosophila yakuba (Fruit fly)
Taxonomic identifieri7245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000002282 Componentsi: Chromosome 2R, Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0013165. Dyak\Amy-d.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000000136719 – 494Alpha-amylase BAdd BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi153 ↔ 167By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7245.FBpp0256658.

Structurei

3D structure databases

ProteinModelPortaliQ9BN01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
KOiK01176.
OMAiSQVRDCR.
OrthoDBiEOG091G07V6.
PhylomeDBiQ9BN01.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BN01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL
60 70 80 90 100
GPNGFAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEQQFASMVK
110 120 130 140 150
RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSTKSFP GVPYSSLDFN
160 170 180 190 200
PTCSINNYND ANQVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG
210 220 230 240 250
FRVDAAKHMW PADLAVIYGR LKTLNTDHGF NSGSKAYIVQ EVIDMGGEAI
260 270 280 290 300
SKSEYTGLGA VTEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV
310 320 330 340 350
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF
360 370 380 390 400
SDTDQGPPTT DGHNIASPVF NSDNSCSGGW VCEHRWRQIY NMVAFRNAVG
410 420 430 440 450
SDAIQNWWDN GSNQIAFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV
460 470 480 490
ISGSKSGSSC TGKTVSVGSD GRASIYLGSS EDDGVLAIHV NAKL
Length:494
Mass (Da):53,688
Last modified:March 29, 2004 - v2
Checksum:iEBDC615F3C0044B8
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti179 – 180NS → DP in strain: LO4. 2
Natural varianti195D → N in strain: LO4. 1
Natural varianti330A → V in strain: LO4. 1
Natural varianti381V → A in strain: LO4. 1
Natural varianti405Q → P in strain: LO4. 1
Natural varianti427F → Y in strain: LO4. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17737 Genomic DNA. Translation: BAA04590.1.
AF280886 Genomic DNA. Translation: AAG60011.1.
AF280887 Genomic DNA. Translation: AAG60012.1.
AF280888 Genomic DNA. Translation: AAG60013.1.
AF280889 Genomic DNA. Translation: AAG60014.1.
CM000158 Genomic DNA. Translation: EDW92184.1.
PIRiS58946.
RefSeqiXP_002092472.1. XM_002092436.2.

Genome annotation databases

EnsemblMetazoaiFBtr0258166; FBpp0256658; FBgn0013165.
GeneIDi6531681.
KEGGidya:Dyak_GE11648.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17737 Genomic DNA. Translation: BAA04590.1.
AF280886 Genomic DNA. Translation: AAG60011.1.
AF280887 Genomic DNA. Translation: AAG60012.1.
AF280888 Genomic DNA. Translation: AAG60013.1.
AF280889 Genomic DNA. Translation: AAG60014.1.
CM000158 Genomic DNA. Translation: EDW92184.1.
PIRiS58946.
RefSeqiXP_002092472.1. XM_002092436.2.

3D structure databases

ProteinModelPortaliQ9BN01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7245.FBpp0256658.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0258166; FBpp0256658; FBgn0013165.
GeneIDi6531681.
KEGGidya:Dyak_GE11648.

Organism-specific databases

FlyBaseiFBgn0013165. Dyak\Amy-d.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
KOiK01176.
OMAiSQVRDCR.
OrthoDBiEOG091G07V6.
PhylomeDBiQ9BN01.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYB_DROYA
AccessioniPrimary (citable) accession number: Q9BN01
Secondary accession number(s): B4P8G7, P51548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 29, 2004
Last modified: October 5, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.