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Q9BN01

- AMYB_DROYA

UniProt

Q9BN01 - AMYB_DROYA

Protein

Alpha-amylase B

Gene

Amy-d

Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (29 Mar 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity
    Binds 1 chloride ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi116 – 1161CalciumBy similarity
    Metal bindingi165 – 1651Calcium; via carbonyl oxygenBy similarity
    Metal bindingi174 – 1741CalciumBy similarity
    Binding sitei202 – 2021ChlorideBy similarity
    Active sitei204 – 2041By similarity
    Metal bindingi208 – 2081Calcium; via carbonyl oxygenBy similarity
    Active sitei241 – 2411By similarity
    Binding sitei304 – 3041ChlorideBy similarity
    Sitei306 – 3061Transition state stabilizerBy similarity
    Binding sitei343 – 3431ChlorideBy similarity

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Calcium, Chloride, Metal-binding

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-amylase B (EC:3.2.1.1)
    Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Gene namesi
    Name:Amy-d
    ORF Names:GE11648
    OrganismiDrosophila yakuba (Fruit fly)
    Taxonomic identifieri7245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000002282: Chromosome 2R, UP000002282: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0013165. Dyak\Amy-d.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818By similarityAdd
    BLAST
    Chaini19 – 494476Alpha-amylase BPRO_0000001367Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Pyrrolidone carboxylic acidBy similarity
    Disulfide bondi46 ↔ 102By similarity
    Disulfide bondi153 ↔ 167By similarity
    Disulfide bondi376 ↔ 382By similarity
    Disulfide bondi448 ↔ 460By similarity

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi7245.FBpp0256658.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BN01.
    SMRiQ9BN01. Positions 21-494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0366.
    KOiK01176.
    OrthoDBiEOG7RJPR2.
    PhylomeDBiQ9BN01.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00110. ALPHAAMYLASE.
    SMARTiSM00642. Aamy. 1 hit.
    SM00632. Aamy_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9BN01-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLAKSIVCL ALLAVANAQF DTNYASGRSG MVHLFEWKWD DIAAECENFL    50
    GPNGFAGVQV SPVNENAVKD SRPWWERYQP ISYKLETRSG NEQQFASMVK 100
    RCNAVGVRTY VDVVFNHMAA DGGTYGTGGS TASPSTKSFP GVPYSSLDFN 150
    PTCSINNYND ANQVRNCELV GLRDLNQGNS YVQDKVVEFL DHLIDLGVAG 200
    FRVDAAKHMW PADLAVIYGR LKTLNTDHGF NSGSKAYIVQ EVIDMGGEAI 250
    SKSEYTGLGA VTEFRHSDSI GKVFRGKDQL QYLTNWGTAW GFAASDRSLV 300
    FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF 350
    SDTDQGPPTT DGHNIASPVF NSDNSCSGGW VCEHRWRQIY NMVAFRNAVG 400
    SDAIQNWWDN GSNQIAFSRG SRGFVAFNND NYDLNSSLQT GLPAGTYCDV 450
    ISGSKSGSSC TGKTVSVGSD GRASIYLGSS EDDGVLAIHV NAKL 494
    Length:494
    Mass (Da):53,688
    Last modified:March 29, 2004 - v2
    Checksum:iEBDC615F3C0044B8
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti179 – 1802NS → DP in strain: LO4.
    Natural varianti195 – 1951D → N in strain: LO4.
    Natural varianti330 – 3301A → V in strain: LO4.
    Natural varianti381 – 3811V → A in strain: LO4.
    Natural varianti405 – 4051Q → P in strain: LO4.
    Natural varianti427 – 4271F → Y in strain: LO4.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17737 Genomic DNA. Translation: BAA04590.1.
    AF280886 Genomic DNA. Translation: AAG60011.1.
    AF280887 Genomic DNA. Translation: AAG60012.1.
    AF280888 Genomic DNA. Translation: AAG60013.1.
    AF280889 Genomic DNA. Translation: AAG60014.1.
    CM000158 Genomic DNA. Translation: EDW92184.1.
    PIRiS58946.
    RefSeqiXP_002092472.1. XM_002092436.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0258166; FBpp0256658; FBgn0013165.
    GeneIDi6531681.
    KEGGidya:Dyak_GE11648.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17737 Genomic DNA. Translation: BAA04590.1 .
    AF280886 Genomic DNA. Translation: AAG60011.1 .
    AF280887 Genomic DNA. Translation: AAG60012.1 .
    AF280888 Genomic DNA. Translation: AAG60013.1 .
    AF280889 Genomic DNA. Translation: AAG60014.1 .
    CM000158 Genomic DNA. Translation: EDW92184.1 .
    PIRi S58946.
    RefSeqi XP_002092472.1. XM_002092436.1.

    3D structure databases

    ProteinModelPortali Q9BN01.
    SMRi Q9BN01. Positions 21-494.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7245.FBpp0256658.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0258166 ; FBpp0256658 ; FBgn0013165 .
    GeneIDi 6531681.
    KEGGi dya:Dyak_GE11648.

    Organism-specific databases

    FlyBasei FBgn0013165. Dyak\Amy-d.

    Phylogenomic databases

    eggNOGi COG0366.
    KOi K01176.
    OrthoDBi EOG7RJPR2.
    PhylomeDBi Q9BN01.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00110. ALPHAAMYLASE.
    SMARTi SM00642. Aamy. 1 hit.
    SM00632. Aamy_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular evolution of the duplicated Amy locus in the Drosophila melanogaster species subgroup: concerted evolution only in the coding region and an excess of nonsynonymous substitutions in speciation."
      Shibata H., Yamazaki T.
      Genetics 141:223-236(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Divergence between Drosophila santomea and allopatric or sympatric populations of D. yakuba using paralogous amylase genes and migration scenarios along the Cameroon volcanic line."
      Cariou M.-L., Silvain J.-F., Daubin V., Da Lage J.-L., Lachaise D.
      Mol. Ecol. 10:649-660(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LBV1, LO4, SA2 and SA3.
    3. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tai18E2 / Tucson 14021-0261.01.

    Entry informationi

    Entry nameiAMYB_DROYA
    AccessioniPrimary (citable) accession number: Q9BN01
    Secondary accession number(s): B4P8G7, P51548
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: March 29, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3