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Q9BJF5

- Q9BJF5_TOXGO

UniProt

Q9BJF5 - Q9BJF5_TOXGO

Protein
Submitted name:

Calmodulin-domain protein kinase 1

Gene

CDPK1

Organism
Toxoplasma gondii
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801ATPanalogImported
    Binding sitei112 – 1121ATPanalogImported
    Binding sitei195 – 1951ATPanalogImported
    Metal bindingi368 – 3681Calcium 1Imported
    Metal bindingi370 – 3701Calcium 1Imported
    Metal bindingi372 – 3721Calcium 1Imported
    Metal bindingi374 – 3741Calcium 1; via carbonyl oxygenImported
    Metal bindingi379 – 3791Calcium 1Imported
    Metal bindingi415 – 4151Calcium 2Imported
    Metal bindingi417 – 4171Calcium 2Imported
    Metal bindingi419 – 4191Calcium 2Imported
    Metal bindingi421 – 4211Calcium 2; via carbonyl oxygenImported
    Metal bindingi423 – 4231Calcium 2Imported
    Metal bindingi426 – 4261Calcium 2Imported
    Metal bindingi451 – 4511Calcium 3Imported
    Metal bindingi453 – 4531Calcium 3Imported
    Metal bindingi455 – 4551Calcium 3Imported
    Metal bindingi457 – 4571Calcium 3; via carbonyl oxygenImported
    Metal bindingi462 – 4621Calcium 3Imported
    Metal bindingi485 – 4851Calcium 4Imported
    Metal bindingi487 – 4871Calcium 4Imported
    Metal bindingi489 – 4891Calcium 4Imported
    Metal bindingi491 – 4911Calcium 4; via carbonyl oxygenImported
    Metal bindingi496 – 4961Calcium 4Imported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi59 – 635ATPanalogImported
    Nucleotide bindingi129 – 1313ATPanalogImported

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. calcium ion binding Source: InterPro
    3. protein serine/threonine kinase activity Source: InterPro

    Keywords - Molecular functioni

    KinaseImported, Transferase

    Keywords - Ligandi

    CalciumImported, Metal-bindingImported, Nucleotide-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Calmodulin-domain protein kinase 1Imported
    Gene namesi
    Name:CDPK1Imported
    OrganismiToxoplasma gondiiImported
    Taxonomic identifieri5811 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3HX4X-ray1.95A1-507[»]
    3I79X-ray2.04A30-507[»]
    3I7BX-ray1.99A30-507[»]
    3I7CX-ray1.98A30-507[»]
    3KU2X-ray2.30A1-507[»]
    3MA6X-ray2.50A/B36-315[»]
    3N51X-ray2.10A30-507[»]
    3NYVX-ray1.88A30-507[»]
    3SX9X-ray2.65A30-507[»]
    3SXFX-ray2.04A30-507[»]
    3T3UX-ray2.10A30-507[»]
    3T3VX-ray2.04A30-507[»]
    3UPXX-ray2.27A30-507[»]
    3UPZX-ray2.20A30-507[»]
    3V51X-ray1.95A30-507[»]
    3V5PX-ray2.10A30-507[»]
    3V5TX-ray2.13A30-507[»]
    4IEBX-ray2.05A1-507[»]
    4IFGX-ray2.11A1-507[»]
    4IH8X-ray2.88A1-507[»]
    4IHPX-ray2.27A1-507[»]
    4JBVX-ray1.95A27-507[»]
    4M7NX-ray2.05A1-507[»]
    4M84X-ray2.00A30-507[»]
    4ONAX-ray2.40A30-507[»]
    ProteinModelPortaliQ9BJF5.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9BJF5.

    Family & Domainsi

    Family and domain databases

    Gene3Di1.10.238.10. 3 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9BJF5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGQQESTLGG AAGEPRSRGH AAGTSGGPGD HLHATPGMFV QHSTAIFSDR    50
    YKGQRVLGKG SFGEVILCKD KITGQECAVK VISKRQVKQK TDKESLLREV 100
    QLLKQLDHPN IMKLYEFFED KGYFYLVGEV YTGGELFDEI ISRKRFSEVD 150
    AARIIRQVLS GITYMHKNKI VHRDLKPENL LLESKSKDAN IRIIDFGLST 200
    HFEASKKMKD KIGTAYYIAP EVLHGTYDEK CDVWSTGVIL YILLSGCPPF 250
    NGANEYDILK KVEKGKYTFE LPQWKKVSES AKDLIRKMLT YVPSMRISAR 300
    DALDHEWIQT YTKEQISVDV PSLDNAILNI RQFQGTQKLA QAALLYMGSK 350
    LTSQDETKEL TAIFHKMDKN GDGQLDRAEL IEGYKELMRM KGQDASMLDA 400
    SAVEHEVDQV LDAVDFDKNG YIEYSEFVTV AMDRKTLLSR ERLERAFRMF 450
    DSDNSGKISS TELATIFGVS DVDSETWKSV LSEVDKNNDG EVDFDEFQQM 500
    LLKLCGN 507
    Length:507
    Mass (Da):57,253
    Last modified:June 1, 2001 - v1
    Checksum:i87008C2075A9CBA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF333958 mRNA. Translation: AAG53993.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF333958 mRNA. Translation: AAG53993.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3HX4 X-ray 1.95 A 1-507 [» ]
    3I79 X-ray 2.04 A 30-507 [» ]
    3I7B X-ray 1.99 A 30-507 [» ]
    3I7C X-ray 1.98 A 30-507 [» ]
    3KU2 X-ray 2.30 A 1-507 [» ]
    3MA6 X-ray 2.50 A/B 36-315 [» ]
    3N51 X-ray 2.10 A 30-507 [» ]
    3NYV X-ray 1.88 A 30-507 [» ]
    3SX9 X-ray 2.65 A 30-507 [» ]
    3SXF X-ray 2.04 A 30-507 [» ]
    3T3U X-ray 2.10 A 30-507 [» ]
    3T3V X-ray 2.04 A 30-507 [» ]
    3UPX X-ray 2.27 A 30-507 [» ]
    3UPZ X-ray 2.20 A 30-507 [» ]
    3V51 X-ray 1.95 A 30-507 [» ]
    3V5P X-ray 2.10 A 30-507 [» ]
    3V5T X-ray 2.13 A 30-507 [» ]
    4IEB X-ray 2.05 A 1-507 [» ]
    4IFG X-ray 2.11 A 1-507 [» ]
    4IH8 X-ray 2.88 A 1-507 [» ]
    4IHP X-ray 2.27 A 1-507 [» ]
    4JBV X-ray 1.95 A 27-507 [» ]
    4M7N X-ray 2.05 A 1-507 [» ]
    4M84 X-ray 2.00 A 30-507 [» ]
    4ONA X-ray 2.40 A 30-507 [» ]
    ProteinModelPortali Q9BJF5.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1781862.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q9BJF5.

    Family and domain databases

    Gene3Di 1.10.238.10. 3 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 4 hits.
    PS50222. EF_HAND_2. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Toxoplasma gondii attachment to host cells is regulated by a calmodulin-like domain protein kinase."
      Kieschnick H., Wakefield T., Narducci C.A., Beckers C.
      J. Biol. Chem. 276:12369-12377(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Discovery of Potent and Selective Inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from C. parvum and T. gondii."
      Murphy R.C., Ojo K.K., Larson E.T., Castellanos-Gonzalez A., Perera B.G., Keyloun K.R., Kim J.E., Bhandari J.G., Muller N.R., Verlinde C.L., White A.C., Merritt E.A., Van Voorhis W.C., Maly D.J.
      ACS Med. Chem. Lett. 1:331-335(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 30-507.
    3. "Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium."
      Wernimont A.K., Artz J.D., Finerty P., Lin Y.H., Amani M., Allali-Hassani A., Senisterra G., Vedadi M., Tempel W., Mackenzie F., Chau I., Lourido S., Sibley L.D., Hui R.
      Nat. Struct. Mol. Biol. 17:596-601(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH ATPANALOG AND CALCIUM.
    4. Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS) OF 30-507.
    5. "Crystal structure of kinase domain of TgCDPK1 in presence of 3BrB-PP1."
      Wernimont A.K., Qiu W., Amani M., Artz J.D., Hassani A.A., Senisterra G., Vedadi M., Sibley L.D., Lourido S., Shokat K., Zhang C., Arrowsmith C.H., Edwards A.M., Bountra C., Weigelt J., Bochkarev A., Hui R., Lin Y.H.
      Submitted (MAR-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 36-315.
    6. "Binding modes of non-specific inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from T. gondii and C. parvum."
      Larson E.T., Ojo K.K., Kim J.E., Verlinde C.L.M.J., Buckner F.S., Hol W.G.J., Merritt E.A., Van Voorhis W.C.
      Submitted (JUL-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) OF 30-507.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 30-507.
    8. "Crystal Structure of a Gly128Met mutant of the toxoplasma CDPK, TGME49_101440."
      Wernimont A.K., Artz J.D., El Bakkouri M., Schapira M., Edwards A.M., Arrowsmith C.H., Bountra C., Hui R., Amani M.
      Submitted (DEC-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH ATPANALOG AND CALCIUM.
    9. "Crystal structure of TgCDPK1 with inhibitor bound."
      Structural Genomics Consortium (SGC)
      El Bakkouri M., Tempel W., Crandall I., Massad T., Loppnau P., Graslund S., Bountra C., Arrowsmith C.H., Edwards A.M., Kain K., Hui R.
      Submitted (DEC-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.88 ANGSTROMS).
    10. Cited for: X-RAY CRYSTALLOGRAPHY (2.27 ANGSTROMS).
    11. "Crystal Structure of a Gly128Met mutant of the toxoplasma CDPK, TGME49_101440."
      Wernimont A.K., Artz J.D., El Bakkouri M., Schapira M., Edwards A.M., Arrowsmith C.H., Bountra C., Hui R., Amani M.
      Submitted (AUG-2013) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH ATPANALOG AND CALCIUM.
    12. "Potent and selective inhibitors of CDPK1 from T. gondii and C. parvum based on a 5-aminopyrazole-4-carboxamide scaffold."
      Zhang Z., Ojo K.K., Vidadala R., Huang W., Geiger J.A., Scheele S., Choi R., Reid M.C., Keyloun K.R., Rivas K., Siddaramaiah L.K., Comess K.M., Robinson K.P., Merta P.J., Kifle L., Hol W.G., Parsons M., Merritt E.A.
      , Maly D.J., Verlinde C.L., Van Voorhis W.C., Fan E.
      ACS Med. Chem. Lett. 5:40-44(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 30-507.
    13. "Development of potent and selective Plasmodium falciparum calcium-dependent protein kinase 4 (PfCDPK4) inhibitors that block the transmission of malaria to mosquitoes."
      Vidadala R.S., Ojo K.K., Johnson S.M., Zhang Z., Leonard S.E., Mitra A., Choi R., Reid M.C., Keyloun K.R., Fox A.M., Kennedy M., Silver-Brace T., Hume J.C., Kappe S., Verlinde C.L., Fan E., Merritt E.A., Van Voorhis W.C., Maly D.J.
      Eur. J. Med. Chem. 74:562-573(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 27-507.

    Entry informationi

    Entry nameiQ9BJF5_TOXGO
    AccessioniPrimary (citable) accession number: Q9BJF5
    Secondary accession number(s): B9Q301, B9QPZ6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3