Calmodulin-domain protein kinase 1

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Q9BJF5 (Q9BJF5_TOXGO) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Submitted name:
Calmodulin-domain protein kinase 1 EMBL AAG53993.1

Submitted name:
Calmodulin-domain protein kinase, putative EMBL EEE19357.1
EC=2.7.11.17 EMBL EEE19357.1

Gene names

Name:	CDPK1 EMBL AAG53993.1
ORF Names:	TGGT1_059880 EMBL EEE19357.1, TGVEG_042030 EMBL EEE27562.1

Organism

Toxoplasma gondii [Reference proteome] EMBL AAG53993.1

Taxonomic identifier

5811 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Conoidasida › Coccidia › Eucoccidiorida › Eimeriorina › Sarcocystidae › Toxoplasma

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	ATP-binding RuleBase RU000304 Calcium RuleBase RU000331 PDB 3HX4 Metal-binding PDB 3HX4 Nucleotide-binding
Molecular function	Kinase EMBL AAG53993.1 Transferase
Technical term	3D-structure PDB 3I79 PDB 3N51 PDB 3KU2 PDB 3HX4 PDB 3V51 PDB 3V5P PDB 3UPX PDB 3V5T PDB 3SX9 PDB 3UPZ PDB 3MA6 PDB 3T3V PDB 3NYV PDB 3SXF PDB 3I7B PDB 3T3U PDB 3I7C Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: InterPro calmodulin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

368

Calcium 1 PDB 3HX4

Metal binding

370

Calcium 1 PDB 3HX4

Metal binding

372

Calcium 1 PDB 3HX4

Metal binding

374

Calcium 1; via carbonyl oxygen PDB 3HX4

Metal binding

379

Calcium 1 PDB 3HX4

Metal binding

415

Calcium 2 PDB 3HX4

Metal binding

417

Calcium 2 PDB 3HX4

Metal binding

419

Calcium 2 PDB 3HX4

Metal binding

421

Calcium 2; via carbonyl oxygen PDB 3HX4

Metal binding

423

Calcium 2 PDB 3HX4

Metal binding

426

Calcium 2 PDB 3HX4

Metal binding

451

Calcium 3 PDB 3HX4

Metal binding

453

Calcium 3 PDB 3HX4

Metal binding

455

Calcium 3 PDB 3HX4

Metal binding

457

Calcium 3; via carbonyl oxygen PDB 3HX4

Metal binding

462

Calcium 3 PDB 3HX4

Metal binding

485

Calcium 4 PDB 3HX4

Metal binding

487

Calcium 4 PDB 3HX4

Metal binding

489

Calcium 4 PDB 3HX4

Metal binding

491

Calcium 4; via carbonyl oxygen PDB 3HX4

Metal binding

496

Calcium 4 PDB 3HX4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9BJF5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 87008C2075A9CBA2
Show »

FASTA

507

57,253

        10         20         30         40         50         60 
MGQQESTLGG AAGEPRSRGH AAGTSGGPGD HLHATPGMFV QHSTAIFSDR YKGQRVLGKG 

        70         80         90        100        110        120 
SFGEVILCKD KITGQECAVK VISKRQVKQK TDKESLLREV QLLKQLDHPN IMKLYEFFED 

       130        140        150        160        170        180 
KGYFYLVGEV YTGGELFDEI ISRKRFSEVD AARIIRQVLS GITYMHKNKI VHRDLKPENL 

       190        200        210        220        230        240 
LLESKSKDAN IRIIDFGLST HFEASKKMKD KIGTAYYIAP EVLHGTYDEK CDVWSTGVIL 

       250        260        270        280        290        300 
YILLSGCPPF NGANEYDILK KVEKGKYTFE LPQWKKVSES AKDLIRKMLT YVPSMRISAR 

       310        320        330        340        350        360 
DALDHEWIQT YTKEQISVDV PSLDNAILNI RQFQGTQKLA QAALLYMGSK LTSQDETKEL 

       370        380        390        400        410        420 
TAIFHKMDKN GDGQLDRAEL IEGYKELMRM KGQDASMLDA SAVEHEVDQV LDAVDFDKNG 

       430        440        450        460        470        480 
YIEYSEFVTV AMDRKTLLSR ERLERAFRMF DSDNSGKISS TELATIFGVS DVDSETWKSV 

       490        500 
LSEVDKNNDG EVDFDEFQQM LLKLCGN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Toxoplasma gondii attachment to host cells is regulated by a calmodulin-like domain protein kinase." Kieschnick H., Wakefield T., Narducci C.A., Beckers C. J. Biol. Chem. 276:12369-12377(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Annotation of Toxoplasma gondii GT1." Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GT1.
[3]	"Annotation of Toxoplasma gondii VEG." Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 50861 / VEG.
[4]	Lorenzi H., Inman J., Amedeo P., Brunk B., Roos D., Caler E. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: GT1 EMBL EEE19357.1 and VEG EMBL EEE27562.1.
[5]	"Discovery of Potent and Selective Inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from C. parvum and T. gondii." Murphy R.C., Ojo K.K., Larson E.T., Castellanos-Gonzalez A., Perera B.G., Keyloun K.R., Kim J.E., Bhandari J.G., Muller N.R., Verlinde C.L., White A.C., Merritt E.A., Van Voorhis W.C., Maly D.J. ACS Med. Chem. Lett. 1:331-335(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 30-507.
[6]	"Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium." Wernimont A.K., Artz J.D., Finerty P., Lin Y.H., Amani M., Allali-Hassani A., Senisterra G., Vedadi M., Tempel W., Mackenzie F., Chau I., Lourido S., Sibley L.D., Hui R. Nat. Struct. Mol. Biol. 17:596-601(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH CALCIUM.
[7]	"Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors." Ojo K.K., Larson E.T., Keyloun K.R., Castaneda L.J., Derocher A.E., Inampudi K.K., Kim J.E., Arakaki T.L., Murphy R.C., Zhang L., Napuli A.J., Maly D.J., Verlinde C.L., Buckner F.S., Parsons M., Hol W.G., Merritt E.A., Van Voorhis W.C. Nat. Struct. Mol. Biol. 17:602-607(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS) OF 30-507.
[8]	"Crystal structure of kinase domain of TgCDPK1 in presence of 3BrB-PP1." Wernimont A.K., Qiu W., Amani M., Artz J.D., Hassani A.A., Senisterra G., Vedadi M., Sibley L.D., Lourido S., Shokat K., Zhang C., Arrowsmith C.H., Edwards A.M., Bountra C., Weigelt J., Bochkarev A., Hui R., Lin Y.H. Submitted (MAR-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 36-315.
[9]	"Binding modes of non-specific inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from T. gondii and C. parvum." Larson E.T., Ojo K.K., Kim J.E., Verlinde C.L.M.J., Buckner F.S., Hol W.G.J., Merritt E.A., Van Voorhis W.C. Submitted (JUL-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) OF 30-507.
[10]	"Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1." Larson E.T., Ojo K.K., Murphy R.C., Johnson S.M., Zhang Z., Kim J.E., Leibly D.J., Fox A.M., Reid M.C., Dale E.J., Perera B.G., Kim J., Hewitt S.N., Hol W.G., Verlinde C.L., Fan E., Van Voorhis W.C., Maly D.J., Merritt E.A. J. Med. Chem. 55:2803-2810(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 30-507.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF333958 mRNA. Translation: AAG53993.1.
EQ967506 Genomic DNA. Translation: EEE19357.1.
EQ970706 Genomic DNA. Translation: EEE27562.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HX4	X-ray	1.95	A	1-507	[»]
3I79	X-ray	2.04	A	30-507	[»]
3I7B	X-ray	1.99	A	30-507	[»]
3I7C	X-ray	1.98	A	30-507	[»]
3KU2	X-ray	2.30	A	1-507	[»]
3MA6	X-ray	2.50	A/B	36-315	[»]
3N51	X-ray	2.10	A	30-507	[»]
3NYV	X-ray	1.88	A	30-507	[»]
3SX9	X-ray	2.65	A	30-507	[»]
3SXF	X-ray	2.04	A	30-507	[»]
3T3U	X-ray	2.10	A	30-507	[»]
3T3V	X-ray	2.04	A	30-507	[»]
3UPX	X-ray	2.27	A	30-507	[»]
3UPZ	X-ray	2.20	A	30-507	[»]
3V51	X-ray	1.95	A	30-507	[»]
3V5P	X-ray	2.10	A	30-507	[»]
3V5T	X-ray	2.13	A	30-507	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.238.10. 3 hits.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

Pfam

PF13499. EF_hand_5. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]

SMART

SM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL1781862.

EvolutionaryTrace

Q9BJF5.

Entry information

Entry name

Q9BJF5_TOXGO

Accession

Primary (citable) accession number: Q9BJF5
Secondary accession number(s): B9Q301, B9QPZ6

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

Ontologies

Sequence annotation (Features)

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Other

Entry information