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Q9BJF5

- Q9BJF5_TOXGO

UniProt

Q9BJF5 - Q9BJF5_TOXGO

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Protein
Submitted name:

Calmodulin-domain protein kinase 1

Gene

CDPK1

Organism
Toxoplasma gondii
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801ATP analogImported
Binding sitei112 – 1121ATP analogImported
Binding sitei195 – 1951ATP analogImported
Metal bindingi368 – 3681Calcium 1Imported
Metal bindingi370 – 3701Calcium 1Imported
Metal bindingi372 – 3721Calcium 1Imported
Metal bindingi374 – 3741Calcium 1; via carbonyl oxygenImported
Metal bindingi379 – 3791Calcium 1Imported
Metal bindingi415 – 4151Calcium 2Imported
Metal bindingi417 – 4171Calcium 2Imported
Metal bindingi419 – 4191Calcium 2Imported
Metal bindingi421 – 4211Calcium 2; via carbonyl oxygenImported
Metal bindingi423 – 4231Calcium 2Imported
Metal bindingi426 – 4261Calcium 2Imported
Metal bindingi451 – 4511Calcium 3Imported
Metal bindingi453 – 4531Calcium 3Imported
Metal bindingi455 – 4551Calcium 3Imported
Metal bindingi457 – 4571Calcium 3; via carbonyl oxygenImported
Metal bindingi462 – 4621Calcium 3Imported
Metal bindingi485 – 4851Calcium 4Imported
Metal bindingi487 – 4871Calcium 4Imported
Metal bindingi489 – 4891Calcium 4Imported
Metal bindingi491 – 4911Calcium 4; via carbonyl oxygenImported
Metal bindingi496 – 4961Calcium 4Imported

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi59 – 635ATP analogImported
Nucleotide bindingi129 – 1313ATP analogImported

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. calcium ion binding Source: InterPro
  3. protein serine/threonine kinase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

KinaseImported, Transferase

Keywords - Ligandi

CalciumImported, Metal-bindingImported, Nucleotide-bindingImported

Names & Taxonomyi

Protein namesi
Submitted name:
Calmodulin-domain protein kinase 1Imported
Gene namesi
Name:CDPK1Imported
OrganismiToxoplasma gondiiImported
Taxonomic identifieri5811 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HX4X-ray1.95A1-507[»]
3I79X-ray2.04A30-507[»]
3I7BX-ray1.99A30-507[»]
3I7CX-ray1.98A30-507[»]
3KU2X-ray2.30A1-507[»]
3MA6X-ray2.50A/B36-315[»]
3N51X-ray2.10A30-507[»]
3NYVX-ray1.88A30-507[»]
3SX9X-ray2.65A30-507[»]
3SXFX-ray2.04A30-507[»]
3T3UX-ray2.10A30-507[»]
3T3VX-ray2.04A30-507[»]
3UPXX-ray2.27A30-507[»]
3UPZX-ray2.20A30-507[»]
3V51X-ray1.95A30-507[»]
3V5PX-ray2.10A30-507[»]
3V5TX-ray2.13A30-507[»]
4IEBX-ray2.05A1-507[»]
4IFGX-ray2.11A1-507[»]
4IH8X-ray2.88A1-507[»]
4IHPX-ray2.27A1-507[»]
4JBVX-ray1.95A27-507[»]
4M84X-ray2.00A30-507[»]
4ONAX-ray2.40A30-507[»]
4TZRX-ray2.00A30-507[»]
ProteinModelPortaliQ9BJF5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BJF5.

Family & Domainsi

Phylogenomic databases

InParanoidiQ9BJF5.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BJF5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGQQESTLGG AAGEPRSRGH AAGTSGGPGD HLHATPGMFV QHSTAIFSDR
60 70 80 90 100
YKGQRVLGKG SFGEVILCKD KITGQECAVK VISKRQVKQK TDKESLLREV
110 120 130 140 150
QLLKQLDHPN IMKLYEFFED KGYFYLVGEV YTGGELFDEI ISRKRFSEVD
160 170 180 190 200
AARIIRQVLS GITYMHKNKI VHRDLKPENL LLESKSKDAN IRIIDFGLST
210 220 230 240 250
HFEASKKMKD KIGTAYYIAP EVLHGTYDEK CDVWSTGVIL YILLSGCPPF
260 270 280 290 300
NGANEYDILK KVEKGKYTFE LPQWKKVSES AKDLIRKMLT YVPSMRISAR
310 320 330 340 350
DALDHEWIQT YTKEQISVDV PSLDNAILNI RQFQGTQKLA QAALLYMGSK
360 370 380 390 400
LTSQDETKEL TAIFHKMDKN GDGQLDRAEL IEGYKELMRM KGQDASMLDA
410 420 430 440 450
SAVEHEVDQV LDAVDFDKNG YIEYSEFVTV AMDRKTLLSR ERLERAFRMF
460 470 480 490 500
DSDNSGKISS TELATIFGVS DVDSETWKSV LSEVDKNNDG EVDFDEFQQM

LLKLCGN
Length:507
Mass (Da):57,253
Last modified:June 1, 2001 - v1
Checksum:i87008C2075A9CBA2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF333958 mRNA. Translation: AAG53993.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF333958 mRNA. Translation: AAG53993.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3HX4 X-ray 1.95 A 1-507 [» ]
3I79 X-ray 2.04 A 30-507 [» ]
3I7B X-ray 1.99 A 30-507 [» ]
3I7C X-ray 1.98 A 30-507 [» ]
3KU2 X-ray 2.30 A 1-507 [» ]
3MA6 X-ray 2.50 A/B 36-315 [» ]
3N51 X-ray 2.10 A 30-507 [» ]
3NYV X-ray 1.88 A 30-507 [» ]
3SX9 X-ray 2.65 A 30-507 [» ]
3SXF X-ray 2.04 A 30-507 [» ]
3T3U X-ray 2.10 A 30-507 [» ]
3T3V X-ray 2.04 A 30-507 [» ]
3UPX X-ray 2.27 A 30-507 [» ]
3UPZ X-ray 2.20 A 30-507 [» ]
3V51 X-ray 1.95 A 30-507 [» ]
3V5P X-ray 2.10 A 30-507 [» ]
3V5T X-ray 2.13 A 30-507 [» ]
4IEB X-ray 2.05 A 1-507 [» ]
4IFG X-ray 2.11 A 1-507 [» ]
4IH8 X-ray 2.88 A 1-507 [» ]
4IHP X-ray 2.27 A 1-507 [» ]
4JBV X-ray 1.95 A 27-507 [» ]
4M84 X-ray 2.00 A 30-507 [» ]
4ONA X-ray 2.40 A 30-507 [» ]
4TZR X-ray 2.00 A 30-507 [» ]
ProteinModelPortali Q9BJF5.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1781862.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

InParanoidi Q9BJF5.

Miscellaneous databases

EvolutionaryTracei Q9BJF5.

Family and domain databases

Gene3Di 1.10.238.10. 3 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Toxoplasma gondii attachment to host cells is regulated by a calmodulin-like domain protein kinase."
    Kieschnick H., Wakefield T., Narducci C.A., Beckers C.
    J. Biol. Chem. 276:12369-12377(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Discovery of Potent and Selective Inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from C. parvum and T. gondii."
    Murphy R.C., Ojo K.K., Larson E.T., Castellanos-Gonzalez A., Perera B.G., Keyloun K.R., Kim J.E., Bhandari J.G., Muller N.R., Verlinde C.L., White A.C., Merritt E.A., Van Voorhis W.C., Maly D.J.
    ACS Med. Chem. Lett. 1:331-335(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 30-507.
  3. "Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium."
    Wernimont A.K., Artz J.D., Finerty P., Lin Y.H., Amani M., Allali-Hassani A., Senisterra G., Vedadi M., Tempel W., Mackenzie F., Chau I., Lourido S., Sibley L.D., Hui R.
    Nat. Struct. Mol. Biol. 17:596-601(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH ATP ANALOG AND CALCIUM.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS) OF 30-507.
  5. "Crystal structure of kinase domain of TgCDPK1 in presence of 3BrB-PP1."
    Wernimont A.K., Qiu W., Amani M., Artz J.D., Hassani A.A., Senisterra G., Vedadi M., Sibley L.D., Lourido S., Shokat K., Zhang C., Arrowsmith C.H., Edwards A.M., Bountra C., Weigelt J., Bochkarev A., Hui R., Lin Y.H.
    Submitted (MAR-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 36-315.
  6. "Binding modes of non-specific inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from T. gondii and C. parvum."
    Larson E.T., Ojo K.K., Kim J.E., Verlinde C.L.M.J., Buckner F.S., Hol W.G.J., Merritt E.A., Van Voorhis W.C.
    Submitted (JUL-2010) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) OF 30-507.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 30-507.
  8. "Crystal Structure of a Gly128Met mutant of the toxoplasma CDPK, TGME49_101440."
    Wernimont A.K., Artz J.D., El Bakkouri M., Schapira M., Edwards A.M., Arrowsmith C.H., Bountra C., Hui R., Amani M.
    Submitted (DEC-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH ATP ANALOG AND CALCIUM.
  9. "Crystal structure of TgCDPK1 with inhibitor bound."
    Structural Genomics Consortium (SGC)
    El Bakkouri M., Tempel W., Crandall I., Massad T., Loppnau P., Graslund S., Bountra C., Arrowsmith C.H., Edwards A.M., Kain K., Hui R.
    Submitted (DEC-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.88 ANGSTROMS).
  10. Cited for: X-RAY CRYSTALLOGRAPHY (2.27 ANGSTROMS).
  11. "Potent and selective inhibitors of CDPK1 from T. gondii and C. parvum based on a 5-aminopyrazole-4-carboxamide scaffold."
    Zhang Z., Ojo K.K., Vidadala R., Huang W., Geiger J.A., Scheele S., Choi R., Reid M.C., Keyloun K.R., Rivas K., Siddaramaiah L.K., Comess K.M., Robinson K.P., Merta P.J., Kifle L., Hol W.G., Parsons M., Merritt E.A.
    , Maly D.J., Verlinde C.L., Van Voorhis W.C., Fan E.
    ACS Med. Chem. Lett. 5:40-44(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 30-507.
  12. "Development of potent and selective Plasmodium falciparum calcium-dependent protein kinase 4 (PfCDPK4) inhibitors that block the transmission of malaria to mosquitoes."
    Vidadala R.S., Ojo K.K., Johnson S.M., Zhang Z., Leonard S.E., Mitra A., Choi R., Reid M.C., Keyloun K.R., Fox A.M., Kennedy M., Silver-Brace T., Hume J.C., Kappe S., Verlinde C.L., Fan E., Merritt E.A., Van Voorhis W.C., Maly D.J.
    Eur. J. Med. Chem. 74:562-573(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 27-507.
  13. "Calcium-Dependent Protein Kinase 1 from Toxoplasma gondii (TgCDPK1) in complex with inhibitor UW1561."
    Merritt E.A., Maly D.J., Ojo K.K., Vidadala R.
    Submitted (JUL-2014) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 30-507.

Entry informationi

Entry nameiQ9BJF5_TOXGO
AccessioniPrimary (citable) accession number: Q9BJF5
Secondary accession number(s): B9Q301, B9QPZ6
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 2001
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3