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Q9BJF5 (Q9BJF5_TOXGO) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
Gene names
Name:CDPK1 EMBL AAG53993.1
OrganismToxoplasma gondii EMBL AAG53993.1
Taxonomic identifier5811 [NCBI]
Taxonomic lineageEukaryotaAlveolataApicomplexaConoidasidaCoccidiaEucoccidioridaEimeriorinaSarcocystidaeToxoplasma

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region209 – 2102Sulfate 3 binding PDB 3HX4

Sites

Metal binding3681Calcium 1 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding3701Calcium 1 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding3721Calcium 1 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding3741Calcium 1; via carbonyl oxygen PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding3791Calcium 1 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4151Calcium 2 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4171Calcium 2 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4191Calcium 2 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4211Calcium 2; via carbonyl oxygen PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4231Calcium 2 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4261Calcium 2 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4511Calcium 3 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4531Calcium 3 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4551Calcium 3 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4571Calcium 3; via carbonyl oxygen PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4621Calcium 3 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4851Calcium 4 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4871Calcium 4 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4891Calcium 4 PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4911Calcium 4; via carbonyl oxygen PDB 3HX4 PDB 4M7N PDB 4IEB
Metal binding4961Calcium 4 PDB 3HX4 PDB 4M7N PDB 4IEB
Binding site1321Sulfate 2 PDB 3HX4
Binding site1731Sulfate 3 PDB 3HX4
Binding site1761Sulfate 4 PDB 3HX4
Binding site1851Sulfate 2; via amide nitrogen PDB 3HX4
Binding site3381Sulfate 1 PDB 3HX4
Binding site4731Sulfate 5 PDB 3HX4

Sequences

Sequence LengthMass (Da)Tools
Q9BJF5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 87008C2075A9CBA2

FASTA50757,253
        10         20         30         40         50         60 
MGQQESTLGG AAGEPRSRGH AAGTSGGPGD HLHATPGMFV QHSTAIFSDR YKGQRVLGKG 

        70         80         90        100        110        120 
SFGEVILCKD KITGQECAVK VISKRQVKQK TDKESLLREV QLLKQLDHPN IMKLYEFFED 

       130        140        150        160        170        180 
KGYFYLVGEV YTGGELFDEI ISRKRFSEVD AARIIRQVLS GITYMHKNKI VHRDLKPENL 

       190        200        210        220        230        240 
LLESKSKDAN IRIIDFGLST HFEASKKMKD KIGTAYYIAP EVLHGTYDEK CDVWSTGVIL 

       250        260        270        280        290        300 
YILLSGCPPF NGANEYDILK KVEKGKYTFE LPQWKKVSES AKDLIRKMLT YVPSMRISAR 

       310        320        330        340        350        360 
DALDHEWIQT YTKEQISVDV PSLDNAILNI RQFQGTQKLA QAALLYMGSK LTSQDETKEL 

       370        380        390        400        410        420 
TAIFHKMDKN GDGQLDRAEL IEGYKELMRM KGQDASMLDA SAVEHEVDQV LDAVDFDKNG 

       430        440        450        460        470        480 
YIEYSEFVTV AMDRKTLLSR ERLERAFRMF DSDNSGKISS TELATIFGVS DVDSETWKSV 

       490        500 
LSEVDKNNDG EVDFDEFQQM LLKLCGN 

« Hide

References

[1]"Toxoplasma gondii attachment to host cells is regulated by a calmodulin-like domain protein kinase."
Kieschnick H., Wakefield T., Narducci C.A., Beckers C.
J. Biol. Chem. 276:12369-12377(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Discovery of Potent and Selective Inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from C. parvum and T. gondii."
Murphy R.C., Ojo K.K., Larson E.T., Castellanos-Gonzalez A., Perera B.G., Keyloun K.R., Kim J.E., Bhandari J.G., Muller N.R., Verlinde C.L., White A.C., Merritt E.A., Van Voorhis W.C., Maly D.J.
ACS Med. Chem. Lett. 1:331-335(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 30-507.
[3]"Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium."
Wernimont A.K., Artz J.D., Finerty P., Lin Y.H., Amani M., Allali-Hassani A., Senisterra G., Vedadi M., Tempel W., Mackenzie F., Chau I., Lourido S., Sibley L.D., Hui R.
Nat. Struct. Mol. Biol. 17:596-601(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH CALCIUM AND SULFATE.
[4]"Toxoplasma gondii calcium-dependent protein kinase 1 is a target for selective kinase inhibitors."
Ojo K.K., Larson E.T., Keyloun K.R., Castaneda L.J., Derocher A.E., Inampudi K.K., Kim J.E., Arakaki T.L., Murphy R.C., Zhang L., Napuli A.J., Maly D.J., Verlinde C.L., Buckner F.S., Parsons M., Hol W.G., Merritt E.A., Van Voorhis W.C.
Nat. Struct. Mol. Biol. 17:602-607(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS) OF 30-507.
[5]"Crystal structure of kinase domain of TgCDPK1 in presence of 3BrB-PP1."
Wernimont A.K., Qiu W., Amani M., Artz J.D., Hassani A.A., Senisterra G., Vedadi M., Sibley L.D., Lourido S., Shokat K., Zhang C., Arrowsmith C.H., Edwards A.M., Bountra C., Weigelt J., Bochkarev A., Hui R., Lin Y.H.
Submitted (MAR-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 36-315.
[6]"Binding modes of non-specific inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from T. gondii and C. parvum."
Larson E.T., Ojo K.K., Kim J.E., Verlinde C.L.M.J., Buckner F.S., Hol W.G.J., Merritt E.A., Van Voorhis W.C.
Submitted (JUL-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) OF 30-507.
[7]"Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1."
Larson E.T., Ojo K.K., Murphy R.C., Johnson S.M., Zhang Z., Kim J.E., Leibly D.J., Fox A.M., Reid M.C., Dale E.J., Perera B.G., Kim J., Hewitt S.N., Hol W.G., Verlinde C.L., Fan E., Van Voorhis W.C., Maly D.J., Merritt E.A.
J. Med. Chem. 55:2803-2810(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 30-507.
[8]"Crystal Structure of a Gly128Met mutant of the toxoplasma CDPK, TGME49_101440."
Wernimont A.K., Artz J.D., El Bakkouri M., Schapira M., Edwards A.M., Arrowsmith C.H., Bountra C., Hui R., Amani M.
Submitted (DEC-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH CALCIUM.
[9]"Crystal structure of TgCDPK1 with inhibitor bound."
Structural Genomics Consortium (SGC)
El Bakkouri M., Tempel W., Crandall I.E., Massad T., Loppnau P., Graslund S., Bountra C., Arrowsmith C.H., Edwards A.M., Kain C.K., Shokat K.M., Sibley L.D., Hui R.
Submitted (DEC-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.27 ANGSTROMS).
[10]"Crystal Structure of a Gly128Met mutant of the toxoplasma CDPK, TGME49_101440."
Wernimont A.K., Artz J.D., El Bakkouri M., Schapira M., Edwards A.M., Arrowsmith C.H., Bountra C., Hui R., Amani M.
Submitted (AUG-2013) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH CALCIUM.
[11]"Potent and selective inhibitors of CDPK1 from T. gondii and C. parvum based on a 5-aminopyrazole-4-carboxamide scaffold."
Zhang Z., Ojo K.K., Vidadala R., Huang W., Geiger J.A., Scheele S., Choi R., Reid M.C., Keyloun K.R., Rivas K., Siddaramaiah L.K., Comess K.M., Robinson K.P., Merta P.J., Kifle L., Hol W.G., Parsons M., Merritt E.A. expand/collapse author list , Maly D.J., Verlinde C.L., Van Voorhis W.C., Fan E.
ACS Med. Chem. Lett. 5:40-44(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 30-507.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF333958 mRNA. Translation: AAG53993.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3HX4X-ray1.95A1-507[»]
3I79X-ray2.04A30-507[»]
3I7BX-ray1.99A30-507[»]
3I7CX-ray1.98A30-507[»]
3KU2X-ray2.30A1-507[»]
3MA6X-ray2.50A/B36-315[»]
3N51X-ray2.10A30-507[»]
3NYVX-ray1.88A30-507[»]
3SX9X-ray2.65A30-507[»]
3SXFX-ray2.04A30-507[»]
3T3UX-ray2.10A30-507[»]
3T3VX-ray2.04A30-507[»]
3UPXX-ray2.27A30-507[»]
3UPZX-ray2.20A30-507[»]
3V51X-ray1.95A30-507[»]
3V5PX-ray2.10A30-507[»]
3V5TX-ray2.13A30-507[»]
4IEBX-ray2.05A1-507[»]
4IHPX-ray2.27A1-507[»]
4M7NX-ray2.05A1-507[»]
4M84X-ray2.00A30-507[»]
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL1781862.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.238.10. 3 hits.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9BJF5.

Entry information

Entry nameQ9BJF5_TOXGO
AccessionPrimary (citable) accession number: Q9BJF5
Secondary accession number(s): B9Q301, B9QPZ6
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)