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Protein

CD166 antigen

Gene

ALCAM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity). Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Cell adhesion, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
CD166 antigen
Alternative name(s):
Activated leukocyte cell adhesion molecule
CD_antigen: CD166
Gene namesi
Name:ALCAM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein By similarity
  • Cell projectionaxon By similarity
  • Cell projectiondendrite By similarity

  • Note: Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells. Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 527ExtracellularSequence analysisAdd BLAST500
Transmembranei528 – 549HelicalSequence analysisAdd BLAST22
Topological domaini550 – 583CytoplasmicSequence analysisAdd BLAST34

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001465828 – 583CD166 antigenAdd BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 113PROSITE-ProRule annotation
Glycosylationi91N-linked (GlcNAc...)Sequence analysis1
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi157 ↔ 220PROSITE-ProRule annotation
Glycosylationi167N-linked (GlcNAc...)Sequence analysis1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi270 ↔ 313PROSITE-ProRule annotation
Glycosylationi306N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi354 ↔ 392PROSITE-ProRule annotation
Glycosylationi361N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi435 ↔ 485PROSITE-ProRule annotation
Glycosylationi457N-linked (GlcNAc...)Sequence analysis1
Glycosylationi480N-linked (GlcNAc...)Sequence analysis1
Glycosylationi499N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9BH13.
PeptideAtlasiQ9BH13.
PRIDEiQ9BH13.

Expressioni

Tissue specificityi

Constitutively expressed in the autonomic nervous system. Sympathetic and parasympathetic nerve fibers but not myelinated nerve fibers in the spinal nerve.1 Publication

Interactioni

Subunit structurei

Homodimer. Interacts (via extracellular domain) with CD6 (via extracellular domain). Homodimerization and interaction with CD6 involve the same region and cannot occur simultaneously. The affinity for CD6 is much higher than the affinity for self-association. Interacts (via glycosylated extracellular domain) with LGALS1 and LGALS3. Interaction with LGALS1 or LGALS3 inhibits interaction with CD6.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000097.

Structurei

3D structure databases

ProteinModelPortaliQ9BH13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 120Ig-like V-type 1Add BLAST93
Domaini125 – 234Ig-like V-type 2Add BLAST110
Domaini245 – 328Ig-like C2-type 1Add BLAST84
Domaini333 – 409Ig-like C2-type 2Add BLAST77
Domaini416 – 501Ig-like C2-type 3Add BLAST86

Domaini

The CD6 binding site is located in the N-terminal Ig-like domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFQ2. Eukaryota.
ENOG410ZWU9. LUCA.
HOGENOMiHOG000070101.
HOVERGENiHBG050847.
InParanoidiQ9BH13.
KOiK06547.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiPS50835. IG_LIKE. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BH13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKAAPSCR LVFCLLISAT VLRPGLGWYT VNSAYGDTII MPCRVDVPQN
60 70 80 90 100
LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL NLSENYTLSI
110 120 130 140 150
SNAKISDEKR FVCMLVTEDD VFEAPTVVKV FKQPSKPEIV SKAPFLETDK
160 170 180 190 200
LKKLGECISK DSYPDGNITW YRNGKVLQAL EGAVVIIFRK QMDSVTQLYT
210 220 230 240 250
MTSSLEYKTT KADIQMPFTC SVTYYGPSGQ KTVYSEQAVF DIYYPTEQVT
260 270 280 290 300
IQVLPSKNAI KEGDNITLKC LGNGNPPPEE FLFYLPGQPE GIRSSNTYTL
310 320 330 340 350
TDVKRNATGD YKCSLVDKKS MIASAAITVH YLDLSLNPSG EVTKQIGDAL
360 370 380 390 400
PVSCTISASR NATVVWMKDN VKLRSSPSFS SLHYQDAGNY VCETALQEVE
410 420 430 440 450
GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIQWTIT
460 470 480 490 500
GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV
510 520 530 540 550
SAISIPEHDE ADEISDENKE KVNDQAKLIV GIVVGLLLAA LVAGVVYWLY
560 570 580
MKKSKTASKH VNKDLGNMEE NKKLEENNHK TEA
Length:583
Mass (Da):64,766
Last modified:June 1, 2001 - v1
Checksum:i56B69D0C135B5BA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039957 mRNA. Translation: BAB32790.1.
RefSeqiNP_776663.1. NM_174238.1.
UniGeneiBt.107510.

Genome annotation databases

GeneIDi281614.
KEGGibta:281614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039957 mRNA. Translation: BAB32790.1.
RefSeqiNP_776663.1. NM_174238.1.
UniGeneiBt.107510.

3D structure databases

ProteinModelPortaliQ9BH13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000097.

Proteomic databases

PaxDbiQ9BH13.
PeptideAtlasiQ9BH13.
PRIDEiQ9BH13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281614.
KEGGibta:281614.

Organism-specific databases

CTDi214.

Phylogenomic databases

eggNOGiENOG410IFQ2. Eukaryota.
ENOG410ZWU9. LUCA.
HOGENOMiHOG000070101.
HOVERGENiHBG050847.
InParanoidiQ9BH13.
KOiK06547.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiPS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD166_BOVIN
AccessioniPrimary (citable) accession number: Q9BH13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.