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Protein

Peroxiredoxin-2

Gene

PRDX2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.By similarity

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei52Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-BTA-3299685. Detoxification of Reactive Oxygen Species.
R-BTA-5628897. TP53 Regulates Metabolic Genes.

Protein family/group databases

PeroxiBasei4473. Bt2CysPrx02.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-2 (EC:1.11.1.15)
Gene namesi
Name:PRDX2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001350791 – 199Peroxiredoxin-2Add BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52Interchain (with C-173); in linked formBy similarity
Modified residuei113PhosphoserineBy similarity1
Disulfide bondi173Interchain (with C-52); in linked formBy similarity
Modified residuei183PhosphothreonineBy similarity1

Post-translational modificationi

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9BGI3.
PeptideAtlasiQ9BGI3.
PRIDEiQ9BGI3.

Expressioni

Gene expression databases

BgeeiENSBTAG00000012062.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer. Interacts with TIPIN.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000015996.

Structurei

3D structure databases

ProteinModelPortaliQ9BGI3.
SMRiQ9BGI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 165ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
GeneTreeiENSGT00390000004653.
HOGENOMiHOG000022343.
HOVERGENiHBG000286.
InParanoidiQ9BGI3.
KOiK03386.
OMAiFKEVKLC.
OrthoDBiEOG091G0IE5.
TreeFamiTF105181.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR036249. Thioredoxin-like_sf.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BGI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACVCKAHVG KPAPEFQATA VVDGAFKEVK LSDYKGKYVV LFFYPLDFTF
60 70 80 90 100
VCPTEIVAFS DRAAEFHKLN CEVLGVSVDS QFTHLAWINT PRKEGGLGPL
110 120 130 140 150
NIPLLADVTR KLSSDYGVLK EDEGIAYRGL FVIDGKGVLR QVTINDLPVG
160 170 180 190
RSVDEALRLV QAFQYTDEHG EVCPAGWTPG SDTIKPNVDD SKEYFSKHN
Length:199
Mass (Da):21,946
Last modified:June 1, 2001 - v1
Checksum:i5F256CE54090E2DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305562 mRNA. Translation: AAG53659.1.
BC102351 mRNA. Translation: AAI02352.1.
RefSeqiNP_777188.1. NM_174763.2.
XP_005208710.1. XM_005208653.1.
XP_010805021.1. XM_010806719.2.
UniGeneiBt.2689.

Genome annotation databases

EnsembliENSBTAT00000015996; ENSBTAP00000015996; ENSBTAG00000012062.
GeneIDi286793.
KEGGibta:286793.

Similar proteinsi

Entry informationi

Entry nameiPRDX2_BOVIN
AccessioniPrimary (citable) accession number: Q9BGI3
Secondary accession number(s): Q3T0L0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2001
Last modified: October 25, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families