Reviewed, UniProtKB/Swiss-Prot Q9BGI2 (PRDX4_BOVIN)

Names and origin

Protein names

Recommended name:
    Peroxiredoxin-4
    EC=1.11.1.15
Alternative name(s):
    Prx-IV

Gene names

Name:

PRDX4

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Homodimer or heterodimer with PRDX1; disulfide-linked, upon oxidation By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is the redox-active Cys-127 oxidized to Cys-SOH. Cys-SOH rapidly reacts with Cys-248-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin By similarity. Irreversibly inactivated by overoxidation of Cys-127 (to Cys-SO3H) upon oxidative stress By similarity.
Sequence similarities	Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Peroxiredoxin-4		PRO_0000135097
Regions
	Domain	82 – 240	159	Thioredoxin
Sites
	Active site	127	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	127		Interchain (with C-248); in linked form By similarity
	Disulfide bond	248		Interchain (with C-127); in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9BGI2-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: AE14176CF1C70E37	FASTA	274	30,741
	10 20 30 40 50 60 MEAPPPPPPL PATTLAPGRS RKLLLLPLLL FLLRAEAVRG FEAEERPRTR EEECHFYAGG 70 80 90 100 110 120 QVYPGEVSRV SVAEHSLHLS KAKISKPAPY WEGTAVINGE FKELKLTDYR GKYLVFFFYP 130 140 150 160 170 180 LDFTFVCPTE IIAFGDRIDE FRSINTEVVA CSVDSQFTHL AWINTPRRQG GLGSINIPLL 190 200 210 220 230 240 ADLNHQISKD YGVYLEDSGH TLRGLFIIDD KGILRQITLN DLPVGRSVDE TLRLVQAFQY 250 260 270 TDKHGEVCPA GWKPGSETII PDPAGKLKYF DKLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of 4 new bovine peroxiredoxins, and screening of the complete peroxiredoxin family in different bovine tissues." Leyens G., Donnay I., Knoops B. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF305563 mRNA. Translation: AAG53660.1. BC109824 mRNA. Translation: AAI09825.1.
IPI	IPI00691994.
RefSeq	NP_776858.1.
UniGene	Bt.5203
3D structure databases
HSSP	HSSP built from PDB template 1QQ2 based on UniProtKB Q63716.
ModBase	Search...
Protein family/group databases
PeroxiBase	4531. Bt2CysPrx04.
Genome annotation databases
Ensembl	ENSBTAG00000006165. Bos taurus. [Contig view]
GeneID	281999.
KEGG	bta:281999.
Phylogenomic databases
HOVERGEN	Q9BGI2.
OMA	Q9BGI2. AINTEVV.
Enzyme and pathway databases
BRENDA	1.11.1.15. 251.
Family and domain databases
InterPro	IPR000866. Alkyl_hydroperoxide_Rdtase. IPR019479. Peroxiredoxin_C. IPR017936. Thioredoxin-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF10417. 1-cysPrx_C. 1 hit. PF00578. AhpC-TSA. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRDX4_BOVIN

Accession

Primary (citable) accession number: Q9BGI2
Secondary accession number(s): Q32L06

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)