Reviewed, UniProtKB/Swiss-Prot Q9BGI0 (GABT_BOVIN)

Names and origin

Protein names

Recommended name:
    4-aminobutyrate aminotransferase, mitochondrial
    EC=2.6.1.19
Alternative name(s):
    Gamma-amino-N-butyrate transaminase
      Short name=GABA transaminase
      Short name=GABA-T
    GABA aminotransferase
      Short name=GABA-AT
    L-AIBAT
    (S)-3-amino-2-methylpropionate transaminase
    EC=2.6.1.22

Gene names

Name:	ABAT
Synonyms:	GABAT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine By similarity.
Catalytic activity	4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate. (S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	behavioral response to cocaine Inferred from sequence or structural similarity. Source: UniProtKB gamma-aminobutyric acid catabolic process Ref.1 Inferred by curator. Source: UniProtKB neurotransmitter catabolic process Ref.1 Inferred by curator. Source: UniProtKB
Cellular component	4-aminobutyrate transaminase complex Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	(S)-3-amino-2-methylpropionate transaminase activity Inferred from electronic annotation. Source: EC 4-aminobutyrate transaminase activity Ref.1 Inferred from electronic annotation. Source: EC protein homodimerization activity Ref.1 Non-traceable author statement. Source: UniProtKB pyridoxal phosphate binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion By similarity
	Chain	29 – 500	472	4-aminobutyrate aminotransferase, mitochondrial		PRO_0000001248
Amino acid modifications
	Modified residue	318	1	N6-acetyllysine By similarity
	Modified residue	357	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9BGI0-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: DC622157B9326CBA	FASTA	500	56,731
	10 20 30 40 50 60 MASMLVAQRL ACSFQHSYRL LVPGSRHISQ AAAKVDVEFD YDGRLMKTEV PGLRCQELMK 70 80 90 100 110 120 QLNIIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI SSVPIGYSHP GLLKLIQQPQ 130 140 150 160 170 180 NASMFVNRPA LGILLPENFV EKLRQSLLSV APKGMSQLIT MACGSCSNEN GLKTIFMWYR 190 200 210 220 230 240 SKERGQRGFP QEELETCMIN QAPWCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS 250 260 270 280 290 300 FDWPIAPFPR LKYPLEEFVK ENQQEEARCL EEVEDLIVKY RKKKKTVAGI IVEPIQSEGG 310 320 330 340 350 360 DNHASDDFFR KLRDIPRKQC CAFLVDVVQT GGGCTGKFWA HEHWARDDPE DVMTSSKKMM 370 380 390 400 410 420 TGGFFHKEEF RPNAPYRIFN TWLGDPSKNL LLAEVINIIK REDLLNNAAH AGKALLTGLL 430 440 450 460 470 480 DLQARYPQFI SRVRGRGTFC SFDTPDDSIR NKLILIARNK GVVLGGCGDK SIRFRPTLVF 490 500 RDHHAHLFLN IFSDILADFK

References

[1]

"Molecular cloning and functional expression of bovine brain GABA transaminase." Jeon S.G., Bahn J.H., Jang J.S., Jang S.H., Lee B.R., Lee K.S., Park J., Kang T.C., Won M.H., Kim H.B., Kwo O.S., Cho S.W., Choi S.Y. Mol. Cells 12:91-96(2001) [PubMed: 11561735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF305692 mRNA. Translation: AAG59861.1.
IPI	IPI00691472.
RefSeq	NP_803458.1.
UniGene	Bt.7219
3D structure databases
SMR	Q9BGI0. Positions 39-499.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9BGI0.
Genome annotation databases
Ensembl	ENSBTAT00000005280; ENSBTAP00000005280; ENSBTAG00000004038; Bos taurus. [Genome view]
GeneID	280969.
KEGG	bta:280969.
Organism-specific databases
CTD	280969.
Phylogenomic databases
eggNOG	maNOG04984.
HOVERGEN	Q9BGI0.
InParanoid	Q9BGI0.
PhylomeDB	Q9BGI0.
Enzyme and pathway databases
BRENDA	2.6.1.19. 251. 2.6.1.22. 251.
Family and domain databases
InterPro	IPR004631. 4NH2But_aminotransferase_euk. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF6. GABAtrns_euk. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00699. GABAtrns_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GABT_BOVIN

Accession

Primary (citable) accession number: Q9BGI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)